Download - Frontal Analysis or Displacement
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Frontal Analysis or DisplacementFrontal Analysis ConceptsContinuously add sample to front of column
Monitor components as they elute (exit from column)Provides a general measure of how compounds are retained on columnExample - Charcoal flitration
Frontal Analysis ProcessMaterials (solute) move down a column of packed material by being
displaced by a more highly retained solute
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Partitioning
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Theory of Partitioning
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W1/2
W
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Retention Factor
Selectivity Factor
A B
Resolution
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time
Detector response
Does N depend on the order of elution?
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A =
B/u =
C*u =
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What’s the optimum mobile phase velocity?
When an acceptable level of separation is achieved, what
choice does the experimenter have regarding optimum mobile
phase velocity?
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