frontal analysis or displacement
DESCRIPTION
Frontal Analysis or Displacement. Frontal Analysis Concepts Continuously add sample to front of column Monitor components as they elute (exit from column) Provides a general measure of how compounds are retained on column Example - Charcoal flitration. Frontal Analysis Process - PowerPoint PPT PresentationTRANSCRIPT
Frontal Analysis or DisplacementFrontal Analysis ConceptsContinuously add sample to front of column
Monitor components as they elute (exit from column)Provides a general measure of how compounds are retained on columnExample - Charcoal flitration
Frontal Analysis ProcessMaterials (solute) move down a column of packed material by being
displaced by a more highly retained solute
Partitioning
Theory of Partitioning
W1/2
W
Retention Factor
Selectivity Factor
A B
Resolution
time
Detector response
Does N depend on the order of elution?
A =
B/u =
C*u =
What’s the optimum mobile phase velocity?
When an acceptable level of separation is achieved, what
choice does the experimenter have regarding optimum mobile
phase velocity?