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GuidelinesforPreparingandSubmittingPooledLibraries
totheOxfordGenomicsCentreThere are a series of basic steps to follow to ensure safe delivery of samples andtimelyinitiationofyourproject.Thisguidelinewillwalkyouthroughthesestepsanddirectyoutotheappropriatedocumentstoreadforyourparticularprojectneeds.
Failuretofollowtheseguidelinesandincorrectsubmissionofsampleswill:
1. Delaytheinitiationofyourproject2. Putyoursamplesatrisk3. Resultinadditionalchargesbeingappliedtoyourproject
Ifindoubtpleaseconsultyourprojectmanager.SampleSubmissionOverviewTheschematicbelowisdesignedtogiveyouanoverviewofthestepsthatyouarerequired to take to ensure the necessary amount of material is provided in thecorrectcontainer,attherighttemperatureandwithalltherequiredpaperwork.Following these guidelines will protect your samples, avoid delays in projectsinitiationandpreventadditionalchargesforprocessing.
1.QCLibraries
2.PlaceintheCorrectContainer
3.CompleteRequiredPaperWork
4.Package
DelivertoWHG ShipSamplestoWHG
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1. LibraryQCGuidelinesToensuresuccessfulpreparationoflibrariesasetofguidelineshavebeenpreparedthatdescribetheminimumamountandqualityofmaterialrequired.
1. Pre-preparedLibrariesQCTheseguidelinescanbefoundonourwebsitehttp://www.well.ox.ac.uk/ogc/documents
2. PlaceLibrariesinTubesTubes:PleaseuseEppendorfLoBind1.5mlmicrocentrifuge tubes.Tubesshouldbepackaged together ina sealedcontainer (egsmallbagorbox)beforeputtingontodryice.Thesealedcontainermustbelabeledwithyournameandquoteorprojectnumber.
Fortubes,ensurealllibrariesareuniquelylabeledandthelabelsmatchthosegivenonthesamplesubmissionform(Eg:Input1,Input2).Customer Sample Name Input 1 Input 2
If the libraries are in thewrong containers,we reserve the right to returnat yourcostortochargeaprocessingfee.Onlyonealiquotofeachlibraryshouldbesubmittedunlessbypriorarrangement.
3. CompletetheAppropriatePaperWork
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4. PackagingLibraries
1. All libraries should be on dry ice if being shipped or if to be left inWHG
receptionarea.SubmittingSamplesforthosewithAccesstoWHGLab3FreezersWhendroppingoffyoursamples
1. Thereisadesignatedfreezerforsampledropoff.ThefreezerislocatedinthefirstbayonthesouthsideofLab3.AplanofLab3andthe locationofthefreezercanbeseeninAppendix1.
2. Samples should be left in this designated area between 9am and 1pm onstandardworkingdays.
This freezer will not be checked other than between these times so werecommendonlytoleavesamplesbetween9amand1pminorderthatyoursamplesarenot lostordefrosted if the freezer isopenedor fails after thistime.
3. The labeled plate or box/bag of tubes should be placed in appropriatedrawer;eithertheoneforDNAortheoneforpre-preparedlibraries.
4. OntopofthefreezerthereisanareainwhichtoleaveRNAsamplesondryice.
After 1pm, nobody will return to check for samples until the followingworkingday.Ifyouareunabletodropoffyoursamplesbetween9amand1pm,please arrange for a colleague todo soorwaituntil the followingworkingday.
1. DeliverorShipLibraries
SubmittingSamples-drop-offpointinWHGreception
1. It will be possible to leave libraries in a cupboard in reception (see imagebelow)between9amand1pmonanyworkingday- this area is not secureandwillbecomewarm,pleaseputallsamplesondryice.
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2. After 1pm, nobody will return to check for samples until the followingworkingday.
3. If you are unable to ship or dropoff your samples in this time slot, pleasewaituntilthenextworkingdayoraskacolleaguetosendthesamplesinyourabsence.
SubmittingSamples-shippingsamples
1. Samples should only be shipped between Monday and Wednesday (or 2workingdaysbeforethestartoftheweekendinthecaseofbankholidays).
2. Allsamplesshouldbeondry ice.Somecouriershavespecificguidelines forshipping samples on dry ice and should be contacted for details prior topackagingupyoursamples.
3. Pleasesendto:
High-ThroughputGenomics(Sequencing),WellcomeCentreforHumanGenetics,RooseveltDrive,Oxford.OX37BN
High-throughputSampleSubmissioncupboard
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Appendix1
Lab3entranceLab3entrance
Submissionfreezer
