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In the Zone: The Relative Efficacy of Antiseptics and Disinfectants
Report of Results with Discussion
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Introduction
6 Bacterial Strains
6 Antibacterial AgentsE. Coli S. Aureus P. VulgarisB. Cereus E. FaecilisP.
Aeruginosa
Hypothesis: That all of the antiseptic and disinfectant agents will be effective against all of the bacterial strains
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Example of Experimental Plate
10% Bleach
Hand Sanitizer
Spic & Span
Mouthwash
Rubbing AlcoholHydrogen Peroxide
Sensi-disk
Agar Plate
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Measuring the Zone of Inhibition
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Results Table
Antibacterial Agent Zone of Inhibition (mm)Bacterial
Strain E. ColiS. Aureus
P. aerugin
P. Vulgaris
B. Cereus
E. Faecilis
Hand Sanitizer 12.5 0 0 0 0 0
Hydrogen Peroxide 1 15.5 2 14 9 15
Mouth Wash 0 0 0 0 0 0
Rubbing Alcohol 0 0 0 0 0 0
10% Bleach 0 0 1 3 0 2
Spic & Span 2 2 0 0 6.5 0
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Graph of Results
E. Coli S. Aureus P. aerugin P. Vulgaris B. Cereus E. Faecilis0
2
4
6
8
10
12
14
16
18
Hand Sanitizer
Hydrogen Peroxide
Mouth Wash
Rubbing Alcohol
10% Bleach
Spic & Span
Bacterial Strain
Dia
met
er o
f Zon
e of
Inhi
bitio
n (m
m)
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Summary of Results
Hydrogen Peroxide was, comparatively, the most consistently and highly effective antibacterial agent tested
Spic and Span as well as bleach come in second and third
Average Zones of InhibitionAntibacterial
AgentAverage Zone of
Inhibition
Hand Sanitizer 2.1
Hydrogen Peroxide 9.4
Mouth Wash 0.0
Rubbing Alcohol 0.0
10% Bleach 1.0
Spic & Span 1.75
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Holistic Analysis
Antibacterial Agent
Average Zone of Inhibition
Consistency Coefficient
(# of bacterial strains effective against/total
bacterial strains)
Total Efficacy Rating
((Coefficient) ( Avg.
Zone))
Hand Sanitizer 2.1 0.17 0.35
Hydrogen Peroxide 9.4 1 9.42
Mouth Wash 0.0 0 0.00
Rubbing Alcohol 0.0 0 0.00
10% Bleach 1.0 0.5 0.50
Spic & Span 1.75 0.5 0.88
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Contrary to Expectations
?
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Acknowledgements
Lab Group Kelly Cinelli Eric Carpizo Dan Dehelian
Lab Instructor Dr. E. Dawley
T.A. Chris Frymoyer
UC Biology Department
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Sources Cited
Image Credits in Order of Appearance Bacteria pictures:
▪ http://www.freedrinkingwater.com/Images-Test/E-Coli01.jpg▪ http://www.vanderbilt.edu/exploration/resources/staph_aureus_800.jpg▪ http://pseudomonas.com/images/paeruginosa.jpg▪ http://www.ciriscience.org/thumbimage.php?id=155▪ https://
encrypted-tbn0.google.com/images?q=tbn:ANd9GcT2cuGZgkF4U7SA7yRW3HDoopkUlbqd-FVmuN6AlHIarWFIJJiYXA
▪ http://upload.wikimedia.org/wikipedia/en/thumb/4/4a/Enterococcus_faecalis20023-300.jpg/220px-Enterococcus_faecalis20023-300.jpg
Antibacterial Pictures:▪ https://
encryptedtbn2.google.com/images?q=tbn:ANd9GcSfNvZpXJob0xfyOAzGAO1NQdtMSAb5IGrU6TpxE0jc89A1RiYn
▪ http://substuff.files.wordpress.com/2010/11/spicspancleaner.jpg▪ http://www.germx.com/data/photos/8oz_Aloe.jpg▪ http://www.mcguffmedical.com/Images/Images550/003208%20Hydrogen%20Peroxide%203
%20Percent,%20Topical%20USP,%2016%20Ounce%20Bottle,%20Each%20McGuffMedical.com.jpg
▪ http://www.scopemouthwash.com/images/crest-products/large/scope-white-mouthwash-mint-splash.png
▪ http://maryl.uibcsites.com/files/2011/05/Alcohol-Isopropyl.jpg
Zone of Inhibition Diagram:▪ Ursinus Bio Department Lab 4 instructions
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Sources Continued
Lab 2.3 & 2.4 Procedure from the Ursinus Bio Department Material Consulted for Discussion
Hand Gels Falter C. B. Science News , Vol. 169, No. 23 (Jun. 10, 2006), p. 366 http://zack2.ursinus.edu:2075/stable/pdfplus/10.1086/503455.pdf?acceptTC=true
▪ Efficacy of Alcohol‐Based Hand Sanitizers Against Fungi and Viruses E. Fendler and P. Groziak Infection Control and Hospital Epidemiology , Vol. 23, No. 2 (February 2002), pp. 61-62