![Page 1: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/1.jpg)
Introduction to Arabidopsis Research
![Page 2: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/2.jpg)
Today’s Goals
Describe the life-cycle of A. thaliana.Set-up a culture of Arabidopsis plants
and grow them to adulthoodExamine examples of how Arabidopsis
thaliana are used experimentally
![Page 3: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/3.jpg)
Arabidopsis thaliana
Small flowering plant Popular as a plant model organism
Plant Bio and GeneticsIn the family of Brassicaceae
Mustard family
![Page 4: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/4.jpg)
A. thaliana
Arabidopsis anatomy www.greenomes.org
Arabidopsis Development www.greenomes.org
![Page 5: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/5.jpg)
As a model organism Most commonly used plant model
Like the fruit flies of the plant world Advantages:
Small size - Max 5cm in diameter, 30cm tall Easy to cultivate - grown in small area Small genome (5 chromosomes, 157 million base pairs,
27,000 genes) Rapid development - about 6 weeks to make mature seed Can self-pollinate Can perform crosses between different strains Many progeny Easy to perform genetic engineering
Can be transformed using Agrobacterium tumefaciens
![Page 6: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/6.jpg)
Commonly Used to Study
Biotechnology ApplicationsLight sensingFlower development
![Page 7: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/7.jpg)
Commonly used related plant
Brassica rapa Wisconsin Fast Plants Can use in classroom VERY easily Several mutant strains available Book on how to work with them
www.fastplants.org
![Page 8: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/8.jpg)
Research Projects
You may choose this organism for your research project
You may choose C. elegansYou may talk with me and choose
something else
![Page 9: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/9.jpg)
Resources TAIR
www.arabidopsis.org Free Mutant strains! Some have uncharacterized phenotypes in terms of
their growth & dev. PREP website
http://www.prep.biotech.vt.edu/expinfo/expinfo_anatomy.html
Suggestions for Experiments Books in my office Brassica rapa - related plant -called “Wisconsin
Fast Plants”
![Page 10: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/10.jpg)
Example
I am interested in investigating salt tolerance in arabidopsis mutants.
My approach is to compare plants with different T-DNA insertions ability to tolerate various concentrations of salt solutions
My Literature search should . . . ?
![Page 11: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/11.jpg)
Our Arabidopsis lab
Learn to cultivate Arabidopsis in both soil and on agar plates Choose 1 parameter to measure Record in lab notebook Prepare you for your independent projects
![Page 12: Introduction to Arabidopsis Research. Today’s Goals Describe the life-cycle of A. thaliana. Set-up a culture of Arabidopsis plants and grow them to adulthood](https://reader036.vdocuments.net/reader036/viewer/2022072014/56649e8a5503460f94b8f152/html5/thumbnails/12.jpg)
Each group of 3
Make 1 bottle of 100mls plant agar.Make it in a 200ml media bottleLabel it:
MS Agar Date Initials