introduction to arabidopsis research. today’s goals describe the life-cycle of a. thaliana. set-up...
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Introduction to Arabidopsis Research
Today’s Goals
Describe the life-cycle of A. thaliana.Set-up a culture of Arabidopsis plants
and grow them to adulthoodExamine examples of how Arabidopsis
thaliana are used experimentally
Arabidopsis thaliana
Small flowering plant Popular as a plant model organism
Plant Bio and GeneticsIn the family of Brassicaceae
Mustard family
A. thaliana
Arabidopsis anatomy www.greenomes.org
Arabidopsis Development www.greenomes.org
As a model organism Most commonly used plant model
Like the fruit flies of the plant world Advantages:
Small size - Max 5cm in diameter, 30cm tall Easy to cultivate - grown in small area Small genome (5 chromosomes, 157 million base pairs,
27,000 genes) Rapid development - about 6 weeks to make mature seed Can self-pollinate Can perform crosses between different strains Many progeny Easy to perform genetic engineering
Can be transformed using Agrobacterium tumefaciens
Commonly Used to Study
Biotechnology ApplicationsLight sensingFlower development
Commonly used related plant
Brassica rapa Wisconsin Fast Plants Can use in classroom VERY easily Several mutant strains available Book on how to work with them
www.fastplants.org
Research Projects
You may choose this organism for your research project
You may choose C. elegansYou may talk with me and choose
something else
Resources TAIR
www.arabidopsis.org Free Mutant strains! Some have uncharacterized phenotypes in terms of
their growth & dev. PREP website
http://www.prep.biotech.vt.edu/expinfo/expinfo_anatomy.html
Suggestions for Experiments Books in my office Brassica rapa - related plant -called “Wisconsin
Fast Plants”
Example
I am interested in investigating salt tolerance in arabidopsis mutants.
My approach is to compare plants with different T-DNA insertions ability to tolerate various concentrations of salt solutions
My Literature search should . . . ?
Our Arabidopsis lab
Learn to cultivate Arabidopsis in both soil and on agar plates Choose 1 parameter to measure Record in lab notebook Prepare you for your independent projects
Each group of 3
Make 1 bottle of 100mls plant agar.Make it in a 200ml media bottleLabel it:
MS Agar Date Initials