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List different movements/motion that occur inside an organism
Session I – How things move inside a cell2/7/14
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A typical highway road map
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http://www.youtube.com/watch?v=wJyUtbn0O5Y
Transport road map of a cell
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Watch (Cell Biology)Measure and Quantify (Systems/Quantitative Biology, Physics)Disable (Geneticists)Analysis/Optimization ( Bio-engineering)
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An effort to understand how these cytoskeletal motor proteins
works inside the cell?
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Why study cytoskeletal motor proteins?
• To understand how living organisms create motion
• Important in every facet of biology
• Relevance to medicine
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Components of molecular machines – Cytoskeletal motors work on tracks
Microtubule track
Motor proteins – Kinesins, Dyneins
Actin track
Motor protein – Myosins
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Microtubules maintain “polarity”
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Microtubules (red)
DNA ( blue)
Actin filaments ( green)
Location of the tracks inside the cell
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+ end
- end
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Kinesin Motor protein
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Kinesin functions- 45 human genes
• Organelle movement (eg Golgi, ER)
• Transport or localization of RNA/proteins
• Mitotic spindle formation and chromosome formation
• Ciliary biogenesis
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Activities - Explain/Teach concepts to your peers
Group 1 – Kinesin walking models. Discuss in vitro experimental observationshttp://www.stanford.edu/group/blocklab/kinesin/kinesin.html
Group 1 presents on 2/14/14
Group 2 – How the motor proteins (Kinesins and Dyneins) convert chemical energy to mechanical energyVale review paper
Group 3 - How to study and perturb microtubule dynamics in vivo? Discuss an assay system to visualize microtubule plus end growth with fluorescent labeling using a particular model systemReview your own literature
Group 2 & 3 presents on 2/21/14