List different movements/motion that occur inside an organism

Session I – How things move inside a cell2/7/14

A typical highway road map

http://www.youtube.com/watch?v=wJyUtbn0O5Y

Transport road map of a cell

Watch (Cell Biology)Measure and Quantify (Systems/Quantitative Biology, Physics)Disable (Geneticists)Analysis/Optimization ( Bio-engineering)

An effort to understand how these cytoskeletal motor proteins

works inside the cell?

Why study cytoskeletal motor proteins?

• To understand how living organisms create motion

• Important in every facet of biology

• Relevance to medicine

Components of molecular machines – Cytoskeletal motors work on tracks

Microtubule track

Motor proteins – Kinesins, Dyneins

Actin track

Motor protein – Myosins

Microtubules maintain “polarity”

Microtubules (red)

DNA ( blue)

Actin filaments ( green)

Location of the tracks inside the cell

+ end

- end

Kinesin Motor protein

Kinesin functions- 45 human genes

• Organelle movement (eg Golgi, ER)

• Transport or localization of RNA/proteins

• Mitotic spindle formation and chromosome formation

• Ciliary biogenesis

Activities - Explain/Teach concepts to your peers

Group 1 – Kinesin walking models. Discuss in vitro experimental observationshttp://www.stanford.edu/group/blocklab/kinesin/kinesin.html

Group 1 presents on 2/14/14

Group 2 – How the motor proteins (Kinesins and Dyneins) convert chemical energy to mechanical energyVale review paper

Group 3 - How to study and perturb microtubule dynamics in vivo? Discuss an assay system to visualize microtubule plus end growth with fluorescent labeling using a particular model systemReview your own literature

Group 2 & 3 presents on 2/21/14