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Locating the Repressor Binding Sites in the
Bacteriophages
• Presented by Sonia Sharma
• BNFO 301
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CONFIDENTIAL2
Repressors What are They
Bacteriophages when infecting the Bacteria have to make an important
decision
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3CONFIDENTIAL
If Life is bestowed
• Lytic promoters have to be silenced• Repressors come to limelight to do
their job• They Block the promoter’s sites for
lytic pathway then proceed to Lysogeny
• Repressor also binds to DNA at specific sequences
• Found multiple times in genome
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CONFIDENTIAL4
Objective is to locate those sites
• By Utilizing• computational tools integrated in BioBIKE,-• A graphical programming interface that enables
biologist to approach complex research questions • Upstream sequences of relevant phages
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CONFIDENTIAL5
Data- available for Che12, e-lambda,L5
and Bxb1
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CONFIDENTIAL6
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CONFIDENTIAL7
Same method for L5 and Bxb1
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CONFIDENTIAL8
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CONFIDENTIAL9
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CONFIDENTIAL10
Testing Time• Apply the same technique to find to
binding sites of some random phages
• Packman• Shaka
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CONFIDENTIAL11
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CONFIDENTIAL12
Concluding thoughtResults yet to be
analyzed