locating the repressor binding sites in the bacteriophages
DESCRIPTION
Presented by Sonia Sharma BNFO 301. Locating the Repressor Binding Sites in the Bacteriophages. Repressors What are They. Bacteriophages when infecting the Bacteria have to make an important decision. If Life is bestowed. Lytic promoters have to be silenced - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/1.jpg)
Locating the Repressor Binding Sites in the
Bacteriophages
• Presented by Sonia Sharma
• BNFO 301
![Page 2: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/2.jpg)
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Repressors What are They
Bacteriophages when infecting the Bacteria have to make an important
decision
![Page 3: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/3.jpg)
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If Life is bestowed
• Lytic promoters have to be silenced• Repressors come to limelight to do
their job• They Block the promoter’s sites for
lytic pathway then proceed to Lysogeny
• Repressor also binds to DNA at specific sequences
• Found multiple times in genome
![Page 4: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/4.jpg)
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Objective is to locate those sites
• By Utilizing• computational tools integrated in BioBIKE,-• A graphical programming interface that enables
biologist to approach complex research questions • Upstream sequences of relevant phages
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Data- available for Che12, e-lambda,L5
and Bxb1
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![Page 7: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/7.jpg)
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Same method for L5 and Bxb1
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![Page 10: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/10.jpg)
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Testing Time• Apply the same technique to find to
binding sites of some random phages
• Packman• Shaka
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![Page 12: Locating the Repressor Binding Sites in the Bacteriophages](https://reader035.vdocuments.net/reader035/viewer/2022081422/5681668b550346895dda4ef5/html5/thumbnails/12.jpg)
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Concluding thoughtResults yet to be
analyzed