Cultivation of bacteria
Media Pure culture
A culture medium
• Water
• Carbon
• Nitrogen,sulphur,phosphorus,oxygen,hydrogen and
• Metal ions, vitamins
Culture medium
• Chemically defined
• Complex
Culture medium
• Liguid
• Solid
Liquid cuture media
• Basal media: Nutrient broth
-Beef extract
-peptone
-NaCl
-Water
Solid medium
• Agar
• Jells at 45º C
• 1.5-2 % agar
• Semisolid agar: 0.5%
Enriched media
• Basal media
• Supplemented with serum, blood or extracts of plant
Differential media
• Blood agar(5%sheep blood+trypticase soy agar)
• Streptococcus pyogenes(Beta-hemolysis)
Selective media
• MacConcey agar
• Endo agar
• Eosin methylene blue(EMB) agar
MacConcey Agar
• Selective for gram-negative bacteria
• Differential for lactose fermentation
Endo agar (E.coli)
Eosin methylene blue(EMB) agar (E.coli)
Pure culture techniques
• Streaking technique
• Pour plate technique
• Spreading technique
a)Aerobic
b)Strict
anaerobic
c)Facultativ
e anaerobic
d)Microaero
philic
e)Aerotolera
nt anaerobic
Anaerobic incubation
Candle jar:capneic incubation
pH of media: neutral
ph of fungal media: slightly acidic
pH of vibrio media: alcaline