Cultivation of bacteria

Media Pure culture

A culture medium

• Water

• Carbon

• Nitrogen,sulphur,phosphorus,oxygen,hydrogen and

• Metal ions, vitamins

Culture medium

• Chemically defined

• Complex

Culture medium

• Liguid

• Solid

Liquid cuture media

• Basal media: Nutrient broth

-Beef extract

-peptone

-NaCl

-Water

Solid medium

• Agar

• Jells at 45º C

• 1.5-2 % agar

• Semisolid agar: 0.5%

Enriched media

• Basal media

• Supplemented with serum, blood or extracts of plant

Differential media

• Blood agar(5%sheep blood+trypticase soy agar)

• Streptococcus pyogenes(Beta-hemolysis)

Selective media

• MacConcey agar

• Endo agar

• Eosin methylene blue(EMB) agar

MacConcey Agar

• Selective for gram-negative bacteria

• Differential for lactose fermentation

MacConcey Agar

Lactose negative

Endo agar (E.coli)

Eosin methylene blue(EMB) agar (E.coli)

Pure culture techniques

• Streaking technique

• Pour plate technique

• Spreading technique

a)Aerobic

b)Strict

anaerobic

c)Facultativ

e anaerobic

d)Microaero

philic

e)Aerotolera

nt anaerobic

Anaerobic incubation

Candle jar:capneic incubation

pH of media: neutral

ph of fungal media: slightly acidic

pH of vibrio media: alcaline