Regional Breeding for Wheat Stripe Rust
Resistance in the Eastern United States
David Marshall
Plant Science Research Unit
Raleigh, NC
The U.S. has the most cost-The U.S. has the most cost-effective and plentiful food effective and plentiful food production and supply production and supply system in the world.system in the world.
Wheat Stripe Rust
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19761978
19801981
19831984
19871990
19992000
20012002
20032004
20052006
IdahoOregon
WashingtonCalifornia
KansasColoradoNebraskaLouisianaArkansasEpidemic Year
% Loss
Stripe Rust Epidemic Years and the States with Greater than 1% Yield Loss from 1976 to 2004.
Objectives:
Identify stripe rust resistance/susceptibility in Eastern U.S. wheat cultivars, elite lines, and other germplasm.
Identify the gene or genes involved resistance.
Introgress stripe rust resistance into elite lines having regional/local adaptation.
Distribute populations and fixed lines to cooperators.
Protocol:
Established the Uniform Eastern Wheat Stripe Rust Nursery. In 2005-06, had up to 20 entries from State breeding programs in FL, GA, NC, VA, MD, NY, MI, MO, IL, IN, KY, AR, and LA; private breeding programs – Westbred, AgriPro-Coker, and Genesis; and USDA/ARS.
Seedling screening in Raleigh, NC.
Field screening in Plains and Griffin, GA; Kinston, NC; Baton Rouge and Winnsboro, LA; Fayetteville, AR; San Antonio, TX, Pullman and Mt. Vernon, WA.
IT %
Designation Pedigree Contributor Source Mean Mean
TERRAL LA841
GA8665-D4/GA85238-C3 Harrison Louisiana 0.00 0.00
GA961567-4A35
Jackson/2*881130 Johnson Georgia 0.00 0.00
GA97531-5A37
891138/Kinsco//PIO 2628 Johnson Georgia 0.00 0.00
LA99042E-62-B
Ducula1(H2O)/Mason,F1//PIO26R61 Harrison Louisiana 0.75 0.03
LA96140BUA70-2
VA88-52-69/TX89D6435//C9134,F1/3/Mason Harrison Louisiana 0.75 0.03
GA951231-4E26
881130/C9134 Johnson Georgia 0.25 0.03
NC03-7222 L910568/NCV93-1007//LA854111 Murphy North Carolina
0.25 0.03
MO 050146 MO 94-046/Pioneer 2552 McKendry Missouri 0.25 0.03
IL87-2834-1 Tyler / Howell Kolb Illinois 0.75 0.03
992133A2-1-2
Patterson/F201R//INW0101/4/Patton/3/Clark*4/Ning7840//Foster
Ohm Indiana 0.25 0.03
Identified 102 lines with resistance better than the "resistant" check (Pioneer 26R61). Infection types less than '3' and severities less than 18%.
Identified adult-plant resistance in at least 8 lines:
LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)
97C-0554-01 VA94-54-549/Roane//Kristy
B010973 L880085/XW502
Coker 9511 L881060/L880436
IL87-2834-1 Tyler / Howell
IL02-5030 P88288C1-1-6-1-2-8 / 96-6472
IL03-18438 97-3574 / 95-4162
INW0412 Huapei 57-2/Patterson
Several lines had combined stripe rust, leaf rust and powdery mildew resistance.
FL91226A-X4 Star'S'(IBW179)/C9105//F302/P2550,F5IDEO
FL98031-D15-E4 8952-B-7/CM98322-0M-2Y,F1//P26R61
LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)
LA98094-BUB-58-5 90185B24-4-3/87167D8-2
GA 96229-3A41 881130/*2 881582
GA961567-4A35 Jackson/2*881130
GA981621-5E34 AGS 2485/PIO 26R61
GA98249G1-5E56 881130/ 2*901146
GA011450DH2-5A45 USG 3592/PIO 26R61
ARS03-3849 TX85-264*2/TTCC512 (T.cylindricum) (=WX02ARS149-8)
VA01W-205 PION2684/93-54-185 (WLR/3/MSY*3/BALKAN//SAL)// POCAHONTAS,F11
B990133 L890145/LX 8728D
INW0412 Huapei 57-2/Patterson
99840C4-8-4 961331/INW0102//F201R/Foster
E01-1053 U90-1A//ZX90-2C1/P2580
Regional Breeding scheme for introducing resistant sources:
A. Backcross-Bulk (minor genes)
1. Several resistant sources x Adapted type
2. Backcross to Adapted type
3. Select in BC1 through F3 for low terminal severities
B. Accelerated Population Development for major genes
1. Resistant source x Adapted type (crosses made in February)
2. F1 planted in NZ in May, F2 in NC in Nov, F3 in NZ in June, F4 in in Nov.