Regional Breeding for Wheat Stripe Rust

Resistance in the Eastern United States

David Marshall

Plant Science Research Unit

Raleigh, NC

The U.S. has the most cost-The U.S. has the most cost-effective and plentiful food effective and plentiful food production and supply production and supply system in the world.system in the world.

Wheat Stripe Rust

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19761978

19801981

19831984

19871990

19992000

20012002

20032004

20052006

IdahoOregon

WashingtonCalifornia

KansasColoradoNebraskaLouisianaArkansasEpidemic Year

% Loss

Stripe Rust Epidemic Years and the States with Greater than 1% Yield Loss from 1976 to 2004.

Objectives:

Identify stripe rust resistance/susceptibility in Eastern U.S. wheat cultivars, elite lines, and other germplasm.

Identify the gene or genes involved resistance.

Introgress stripe rust resistance into elite lines having regional/local adaptation.

Distribute populations and fixed lines to cooperators.

Protocol:

Established the Uniform Eastern Wheat Stripe Rust Nursery. In 2005-06, had up to 20 entries from State breeding programs in FL, GA, NC, VA, MD, NY, MI, MO, IL, IN, KY, AR, and LA; private breeding programs – Westbred, AgriPro-Coker, and Genesis; and USDA/ARS.

Seedling screening in Raleigh, NC.

Field screening in Plains and Griffin, GA; Kinston, NC; Baton Rouge and Winnsboro, LA; Fayetteville, AR; San Antonio, TX, Pullman and Mt. Vernon, WA.

IT %

Designation Pedigree Contributor Source Mean Mean

TERRAL LA841

GA8665-D4/GA85238-C3 Harrison Louisiana 0.00 0.00

GA961567-4A35

Jackson/2*881130 Johnson Georgia 0.00 0.00

GA97531-5A37

891138/Kinsco//PIO 2628 Johnson Georgia 0.00 0.00

LA99042E-62-B

Ducula1(H2O)/Mason,F1//PIO26R61 Harrison Louisiana 0.75 0.03

LA96140BUA70-2

VA88-52-69/TX89D6435//C9134,F1/3/Mason Harrison Louisiana 0.75 0.03

GA951231-4E26

881130/C9134 Johnson Georgia 0.25 0.03

NC03-7222 L910568/NCV93-1007//LA854111 Murphy North Carolina

0.25 0.03

MO 050146 MO 94-046/Pioneer 2552 McKendry Missouri 0.25 0.03

IL87-2834-1 Tyler / Howell Kolb Illinois 0.75 0.03

992133A2-1-2

Patterson/F201R//INW0101/4/Patton/3/Clark*4/Ning7840//Foster

Ohm Indiana 0.25 0.03

Identified 102 lines with resistance better than the "resistant" check (Pioneer 26R61). Infection types less than '3' and severities less than 18%.

Identified adult-plant resistance in at least 8 lines:

LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)

97C-0554-01 VA94-54-549/Roane//Kristy

B010973 L880085/XW502

Coker 9511 L881060/L880436

IL87-2834-1 Tyler / Howell

IL02-5030 P88288C1-1-6-1-2-8 / 96-6472

IL03-18438 97-3574 / 95-4162

INW0412 Huapei 57-2/Patterson

Several lines had combined stripe rust, leaf rust and powdery mildew resistance.

FL91226A-X4 Star'S'(IBW179)/C9105//F302/P2550,F5IDEO

FL98031-D15-E4 8952-B-7/CM98322-0M-2Y,F1//P26R61

LA95171CA58-3-2-C CK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877)

LA98094-BUB-58-5 90185B24-4-3/87167D8-2

GA 96229-3A41 881130/*2 881582

GA961567-4A35 Jackson/2*881130

GA981621-5E34 AGS 2485/PIO 26R61

GA98249G1-5E56 881130/ 2*901146

GA011450DH2-5A45 USG 3592/PIO 26R61

ARS03-3849 TX85-264*2/TTCC512 (T.cylindricum) (=WX02ARS149-8)

VA01W-205 PION2684/93-54-185 (WLR/3/MSY*3/BALKAN//SAL)// POCAHONTAS,F11

B990133 L890145/LX 8728D

INW0412 Huapei 57-2/Patterson

99840C4-8-4 961331/INW0102//F201R/Foster

E01-1053 U90-1A//ZX90-2C1/P2580

Regional Breeding scheme for introducing resistant sources:

A. Backcross-Bulk (minor genes)

1. Several resistant sources x Adapted type

2. Backcross to Adapted type

3. Select in BC1 through F3 for low terminal severities

B. Accelerated Population Development for major genes

1. Resistant source x Adapted type (crosses made in February)

2. F1 planted in NZ in May, F2 in NC in Nov, F3 in NZ in June, F4 in in Nov.