Bulletin 6705 Ver A 15-1225 1115

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Optional

Required

Optional

Required

Ensure loading stage is in Wash position

Click Run QC button

Review QC reportPass: Click check button Fail: Repeat QC

Move loading stage to Run position

Review drop delayChoose correct Bead Lot file

Load tube containing ten drops (0.5 ml) ProLine™ Universal Calibration Beads

Move loading stage to Wash position

Click Shutdown button Move loading stage to Run position

Click Yes to shut down system Click Clean System button

Load tube containing 4 ml cleaning solution (10% bleach or 70% ethanol)

Apply setting required Select High Pressure Clean

When cleaning cycle is complete, move loading stage to Wash position

Review message, then click Yes to continue

Review warning, then click Yes to continue

Move loading stage to Run position

Load tube containing 4 ml detergent or enzyme-based solution

Review warning, then click Yes to continue

Click Start-Up button Select startup time

After clean cycle is complete (2 min), move loading stage to Wash position and remove tube

Quality Control

Now proceed with Step 2 of the Daily Shutdown Procedure.

321

321

5 64

21 3

4 5 6

7 8

21 3

4 5 6

7

Daily Startup and Quality Control

On a daily basis, the S3e Cell Sorter requires approximately 20 minutes of warm-up time before running quality control (QC) or any samples. To warm up the system:

Daily Shutdown Procedure

These steps should be performed after the last sort of the day:

High Pressure Clean

For a more stringent cleaning process, these steps should be performed after the last sort of the day:

Important! Prior to shutdown, make sure that you have saved all open protocols. Important! Prior to shutdown, make sure that you have saved all open protocols.

S3e™ Cell Sorter Daily Activities

S3e™ Cell Sorter Daily Activities

S3eCell Sorter

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