Download - Vascular Injury Panel 2 (human) Kits
Table of Contents
Contact Information MSD Customer Service
MSD Scientific Support
Introduction
µ
Principle of the Assay
Kit Components
Additional Materials and Equipment
Optional Materials and Equipment
Safety
Best Practices and Technical Hints
Reagent Preparation
Protocol
Validation
Analysis of Results
Typical Data
Vascular Injury Panel 2 (human)
1 10 100 1000 10000 100000 1000000
100
1000
10000
100000
1000000
10000000
SAA
CRP
VCAM-1
ICAM-1
Concentration (pg/mL)
Sig
nal
Sensitivity
Precision
Dilution Linearity
% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛
𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100
Spike Recovery
% 𝑅𝑒𝑐𝑜𝑣𝑒𝑟𝑦 =𝑚𝑒𝑎𝑠𝑢𝑟𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛
𝑒𝑥𝑝𝑒𝑐𝑡𝑒𝑑 𝑐𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛× 100
Specificity
% 𝑁𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐𝑖𝑡𝑦 =𝑛𝑜𝑛𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙
𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑠𝑖𝑔𝑛𝑎𝑙× 100
Interference
Stability
Calibration
Tested Samples
Assay Components
References
Appendix A
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1000
10000
100000
1000000
10000000
Wash
Remove Wash
SAA Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 100000100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
CRP Concentration (pg/mL)
Sig
nal
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
VCAM-1 Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Wash
Remove Wash
ICAM-1 Concentration (pg/mL)
Sig
nal
Appendix B
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
SAA Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
CRP Concentration (pg/mL)S
ignal
10 100 1000 10000 100000 100000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
VCAM-1 Concentration (pg/mL)
Sig
nal
1 10 100 1000 10000 10000010
100
1000
10000
100000
1000000
10000000
Multiplex
Individual Assay
ICAM-1 Concentration (pg/mL)
Sig
nal
Summary Protocol
o
o
Catalog Numbers
Plate Diagram