dqcm beating the standard coagulometer in the …...dqcm beating the standard coagulometer in the...
TRANSCRIPT
![Page 1: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/1.jpg)
DQCM Beating the Standard Coagulometer in
the Domain of Sensitivity Range and
Information for Hemostasis of Human Plasma
Dr. Munawar Hussain
Biosensor Research Group
19 May 2014 1
![Page 2: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/2.jpg)
19 May 2014 2
Instrumentation: qCell T
Photograph with permission from 3T Analytik
![Page 3: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/3.jpg)
19 May 2014 3
Instrumentation: qCell T
• 10 MHz QCM (mass and visco-elastic sensitive transducer)
• Equally suitable for real time liquid and gas phase sensing
• Equipped with efficient thermostat with fast temperature control
• Powerful pumping system that eliminates any chance of bubble/
fluctuation in liquid phase analysis
• Fast and excellent baseline stability in few seconds
• The only DQCM instrument suitable for Hemostasis because of
excellent baseline stability
• User friendly software with latest features for data handling
![Page 4: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/4.jpg)
Literature
19 May 2014 4
* Y. Chunyan, Q. Ling, F. Weiling, Sensors. 2013, 13(6), 6946.
* R. S. Lakshmanan, V. Efremov, S. M. Cullen, A. J. Killard, Sens. Act. B. 2014, 192, 23.
• Some reports on fibrinogen concentration calculation
using QCM*
• No method for cumulative study of aPTT with fibrinogen
concentration in a single set of measurement.
• No study on comparison of aPTT with standard
coagulometer.
![Page 5: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/5.jpg)
Experimental
19 May 2014
5
VP-DVB
polymerization
under UV
λmax 350 nm
UV hardened
QCMs
aPTTqCell T
measurementsMeasurements
Spin coated QCMs coated
with thin film
UV λmax 350 nm
5000 rpm
Standard
Coagulometer
![Page 6: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/6.jpg)
19 May 2014 6
DQCM Exemplary aPTT Curve
-750
-600
-450
-300
-150
0
150
300
450
600
0 20 40 60 80
Δ f
/H
zΔ
Γ/
Hz
t/ s
aPTT Coagulation
aPTT CoagulationDamping
Negative Control withPlasma
Negative Control withPlasma Damping
Negative Control withoutPlasma
Negative Control withoutPlasma Damping
aPTT Starter
CaCl2 Addition
aPTT Point
Total Coagulation
![Page 7: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/7.jpg)
19 May 2014 7
aPPT for Heparin Induced Plasma
-1200
-800
-400
0
400
800
1200
1600
0 100 200 300 400 500 600
Δ f
/H
zΔ
Γ/
Hz
t/ s
Plasma
Plasma Damping
Plasma + 0.25 IU/mlHeparin
Plasma + 0.25 IU/mlHeparin Damping
Plasma + 0.5 IU/mlHeparin
Plasma + 0.5 IU/mlHeparin Damping
Plasma + 1 IU/ml Heparin
Plasma + 1 IU/ml HeparinDamping
aPTT Starter
Addition
![Page 8: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/8.jpg)
19 May 2014 8
DQCM Vs Standard Coagulometer
25
50
75
100
125
150
25 50 75 100 125 150
tQC
M/
s
tCoag / s
Plasma
+ 0.25 IU ml-1 Heparin
+ 0.5 IU ml-1 Heparin
![Page 9: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/9.jpg)
19 May 2014 9
aPTT for Heparin 1 IU/mL Plasma
0
50
100
150
200
250
300
1 2 3 4 5 6 7 8 9 10 11
tQC
M/
s
Samples Containing Heparin 1 IU/ml
tCoag / s Not Applicable
![Page 10: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/10.jpg)
19 May 2014 10
Bland Altman Plot
-10.00
-5.00
0.00
5.00
10.00
25 50 75 100 125 150
tQC
M -
tCoa
g /
s
(tQCM + tCoag)/2 / s
Plasma
+ 0.25 IU ml-1 Heparin
+ 0.5 IU ml-1 Heparin
Mean-2SD
Mean
Mean +2SD
![Page 11: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/11.jpg)
19 May 2014 11
Fibrinogen Gradient Curves for PPP
![Page 12: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/12.jpg)
19 May 2014 12
Fibrinogen Calibration Curves
y = 20.755x2 - 30.875x - 59.939
R² = 0.9908
y = 6.4611x2 + 30.514x - 35.362
R² = 0.9983
y = 23.023x2 - 47.686x - 26.988
R² = 0.9767
y = 17.278x2 - 46.529x + 34.267
R² = 0.9757
y = -10.879x2 + 1.7767x - 158.05
R² = 0.9671
y = 1.1489x2 - 82.923x + 51.613
R² = 0.9892
y = -188.9ln(x) - 6.9266
R² = 0.9978
y = -13.209x2 + 41.728x - 146.64
R² = 0.9982
-600
-400
-200
0
200
400
600
0 1.5 3 4.5 6
Δ f
/ H
z
Δ Γ
/ H
z
Plasma Fibrinogen / (g/L)
Δ f /Δ Γ [From Start Point till aPTT Point]
Pl Poor
Pl 0.3 million
Pl 0.15 million
Pl 0.05 million
(A)
-750
-600
-450
-300
-150
0
150
300
450
600
0 20 40 60 80
Δ f
/H
zΔ
Γ/
Hz
t/ s
![Page 13: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/13.jpg)
19 May 2014 13
Fibrinogen Calibration Curves
y = 102.37x2 - 376.61x + 514.95
R² = 0.9772
y = 51.687x2 - 63.232x + 20.824
R² = 0.9995
y = 26.626x2 + 99.158x - 59.395
R² = 0.9982 y = 70.278x2 - 378x + 778.15
R² = 0.9929
y = -33.352x2 + 94.794x - 316.41
R² = 0.9871
y = -9.4979x2 - 53.289x + 55.646
R² = 0.9923
y = -5.2748x2 - 56.587x - 15.148
R² = 0.9753
y = -20.803x2 + 152.62x - 494.77
R² = 0.9972
-1000
-500
0
500
1000
1500
2000
0 1.5 3 4.5 6
Δ f
/ H
z
Δ
Γ/
Hz
Plasma Fibrinogen / (g/L)
Δ f /Δ Γ [From aPTT Point till Total Coagulation]
Pl Poor
Pl 0.3 million
Pl 0.15 million
Pl 0.05 million
(B)
-750
-600
-450
-300
-150
0
150
300
450
600
0 20 40 60 80
Δ f
/H
zΔ
Γ/
Hz
t/ s
![Page 14: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/14.jpg)
19 May 2014 14
Fibrinogen Calibration Curves
y = 123.12x2 - 407.49x + 455.01
R² = 0.9811
y = 58.149x2 - 32.718x - 14.538
R² = 0.9994
y = 49.649x2 + 51.471x - 86.383
R² = 0.9953
y = 87.556x2 - 424.53x + 812.42
R² = 0.9933
y = -44.231x2 + 96.57x - 474.46
R² = 0.9831
y = -8.349x2 - 136.21x + 107.26
R² = 0.9995
y = 6.8344x2 - 209.23x + 123.44
R² = 0.9802
y = -34.012x2 + 194.35x - 641.41
R² = 0.9994
-2000
-1500
-1000
-500
0
500
1000
1500
2000
2500
0 1.5 3 4.5 6
Δ f
/H
z
Δ Γ
/ H
z
Plasma Fibrinogen / (g/L)
Δ f /Δ Γ [From Start Point till Total Coagulation]
Pl Poor
Pl 0.3 million
Pl 0.15 million
Pl 0.05 million
(C)
-750
-600
-450
-300
-150
0
150
300
450
600
0 20 40 60 80
Δ f
/H
zΔ
Γ/
Hz
t/ s
![Page 15: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/15.jpg)
19 May 2014 15
Summary:
Excellent correlation at lower and mid range aPTT with
respect to standard mechanical coagulometer
Plasma-1 IU/mL heparin mechanical coagulometer N/A.
aPTT+Fibrinogen data from a single set of measurements.
Sensing layers are stable can be reused (10 times tested)
qCell T a suitable instrument for coagulation
DQCM has bright future of relevant clinical applications
![Page 16: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/16.jpg)
Thanks for your kind attention
19 May 2014 16
![Page 17: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/17.jpg)
19 May 2014 17
![Page 18: DQCM Beating the Standard Coagulometer in the …...DQCM Beating the Standard Coagulometer in the Domain of Sensitivity Range and Information for Hemostasis of Human Plasma Dr. Munawar](https://reader033.vdocuments.net/reader033/viewer/2022041902/5e6117b8d6308a4afc26ad40/html5/thumbnails/18.jpg)
19 May 2014 18
Fibrinogen Gradient Curves
20
30
40
50
60
70
0 1.5 3 4.5 6
aP
TT
/ s
Plasma Fibrinogen / (g/L)
[Platelets Poor Plasma]
Coagulometer
QCM
WHO Fibrinogen Standard
in Healthy Human Plasma
(A)
20
30
40
50
60
70
0 1.5 3 4.5 6
aP
TT
/ s
Plasma Fibrinogen / (g/L)
[Platelets 0.3 million]
Coagulometer
QCM
WHO Fibrinogen Standard
in Healthy Human Plasma
(B)
20
30
40
50
0 1.5 3 4.5 6
aP
TT
/ s
Plasma Fibrinogen / (g/L)
[Platelets 0.15 million]
Coagulometer
QCM
WHO Fibrinogen Standard
in Healthy Human Plasma
(C)
20
30
40
50
60
0 1.5 3 4.5 6
aP
TT
/ s
Plasma Fibrinogen / (g/L)
[Platelets 0.05 million]
Coagulometer
QCM
WHO Fibrinogen Standard
in Healthy Human Plasma
(D)