International PSC Study Group Meeting, Oslo, 14-15 Jun 2010

Cholangiocarcinoma in PSC: Opportunities within a study group

Dr Steve Pereira

Institute of HepatologyUCL Medical School

&University College Hospital

Characterisation of serum proteins in biliary tract cancer, PSC andIAC using 2D-DIGE and LC-MS/MS

Neomal S Sandanayake1,2,3, John Sinclair3, Fausto Andreola2, Michael H Chapman1,2, Stephane Camuzeaux3, George J Webster1,2, Ian D Norton4 , Ross C Smith5, John F Timms3, Stephen P Pereira1,2

1Department of Gastroenterology and Hepatology, University College London Hospital; 2Institute of Hepatology, University College London; 3Institute for Women's Health, University College London; 4Department of Gastroenterology, 5Department of Surgery, Royal North Shore Hospital, University of Sydney, Australia

Conclusions: Differentially expressed serum proteins were identified in BTC compared to PSC, IAC and healthy controls using serum immuno-affinity depletion, 2DDIGE and LC-MS/MS.

Proposal

Suspicious biliary strictures

ImagingBile / brushingsBloodTissue

Clinical follow for > 1 yr in patients without proven malignant diagnosis

Malignancy Benign disease

Biobank

Patients: >18 yrs Benign or suspected or proven malignant PB diseaseWritten informed consent

Samples: Biliary brushings and bile - in storage buffer (provided)

Blood

- NIH SOPs- inverted 5 times, 1 hour clot at room temp, centrifuged 2200rpm at 4°C, serum aliquoted into 1.5ml cryovials, -80° C

Tissue samples - snap-frozen in liquid N2 and stored at -80°C- FFPE

TRANSBIL: TRANSlational research in BILiary tract cancer

+

Proposal

Pre-clinical samples202,000 women

- annual phlebotomy

270 individuals developed pancreatic cancer

54 individuals developed biliary tract cancer

UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) serum bank at UCL

Resource

Cholangiocarcinoma in PSCOpportunities for trials?

• Surgery / OLT

• Stents

• Radiotherapy / Brachytherapy

• Chemoradiation

• Chemotherapy

• PDT

April 6 2010