ds_dna
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andDteSE
A subsidiary of Omega Diagnostics Group PLC
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What is Anti ds-DNA test
What is SLE
What are the symptoms
Laboratory Studies
This test measures the amount of antibody to double-stranded deoxyribonucleic acid (anti-dsDNA) that may be
present in the blood.Anti-dsDNA is an autoantibody, produced when a person'simmune system fails to distinguish between "self" and "non-self" cellular components. It mistakenly targets and attacksthe body's own genetic material, causing inflammation,tissue damage, and other signs and symptoms that are
associated with an autoimmune disorder.
Anti-dsDNA is one of several antinuclear antibodies(ANA), a group of antibodies directed against substancesfound in the nucleus of cells. While it may be present at alow level with a number of disorders, anti-dsDNA is
primarily associated with the autoimmune disordersystemic lupus erythematosus (SLE or Lupus).
Systemic lupus erythematosus (SLE) is a chronicinflammatory disease that has protean manifestations and
follows a relapsing and remitting course.It is characterized by an autoantibody response to nuclearand cytoplasmic antigens. SLE can affect any organ system,but mainly involves the skin, joints, kidneys, blood cells,and nervous system
The diagnosis of SLE must be based on the properconstellation of clinical findings and laboratory evidence.
SLE can affect the kidneys, joints, blood vessels, skin, heart,lungs, and the brain.
Symptoms may include joint pain, rashes, fatigue, andkidney dysfunction.
SLE occurs most frequently in women between the ages of15 to 40 and is more common in non-Caucasians. While nodirect cause is known, there may be some genetic
predisposition. Certain drugs, chemicals, sunlight, or viralinfections may trigger an episode.
One particularly serious complication of SLE is lupusnephritis, a condition characterized by inflammation of thekidneys, which can lead to protein in the urine, high bloodpressure, and kidney failure. It occurs when theautoantibodies bind to antigens and become deposited inthe kidneys.
Screening laboratory studies to diagnose possible SLE
should include the following:
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Incubations at Room Temperature
ELISA Kit with Break Apart Wells
70 minutes incubation time
Calibrator range 0-400 U/ml for IgG
Color coded ready to use reagents
Microwells coated with human ds-DNA
2.5 IU/ml sensitivity
Latex agglutination test
1 minute protocol
Qualitative test
Positive & negative controls included
Detects serum antinucleoprotein factors