effective dose of euphorbia hirta on staphylococcus aureus-induced
TRANSCRIPT
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Effective Dose of Euphorbia hirta on Staphylococcus aureus-induced Wound infection of Albino Rats
Section 2I Groups 10, 11 & 12
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• natural or alternative medicine > drugs currently out in the market
• Due to less artificial ingredients were used• undergone less several processes than the
commercially-made drugs,• cost-effective.
• Plants - long been used as principal ingredients of traditional medicine in the Philippines
Introduction
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• Among this plant species is Euphorbia hirta L.• leaves are used in the treatment of:
• cough Coryza• hay asthma bronchial infections
• bowel complaints worm infestations• Wound kidney stones• boils
Introduction
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Commonly called snake weed or asthma weed
In the Philippines, known as tawa-tawa or gatas-gatas
alkaloids, tannins and flavonoids as antibacterial properties
Euphorbia hirta
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•General:- To determine the effective dose of
Euphorbia hirta extracts, eliciting its antimicrobial property
•Specific: - To identify other factors that may give
discrepancies that would affect maximum effect of the dose
Objectives
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• cost effective alternative for antimicrobial drugs• to determine susceptibility and resistance of a specific microorganism to Euphorbia hirta• reduced artificial process in producing the agent
Significance
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•East and West Africa: extracts are used in treatment of asthma and respiratory tract inflammations. •Madagascar: used for coughs, chronic bronchitis and other pulmonary disorders. •Angola: widely used in against diarrhea and dysentery, especially amoebic dysentery.Niger: extracts or exudates are used as ear drops and in the treatment of boils, sore and promoting wound healing
Background of the Study
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•Antibacterial effect: Camellia sinensis L. vs. Euphorbia hirta L. against dysentery causing Shigella spp. (Vero cell line) •The antibacterial effects of a methanol extract of E. hirta was demonstrated in vitro using species of Shigella. •The extract was non-cytotoxic and antibacterial.
Background of the Study
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• potential antibacterial activity against 5 medically important bacterial strains: Bacillus subtilis, Staphylococcus epidermidis, Pseudomonas pseudoalcaligenes, Proteus vulgaris and Salmonella typhimurium. • antibacterial activity of aqueous and methanol extracts: determined by agar disk diffusion and agar well diffusion method. •methanol extracts more active than aqueous extracts. •plant extracts were more active against Gram-positive bacteria than against Gram-negative bacteria. •most susceptible bacteria: B. subtilis, S. epidermidis•most resistant bacteria: P. vulgaris, S. typhimurium
Background of the Study
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•Euphorbia hirta ethanol extract is hematologically not toxic to rats•antibacterial activities: presence of tannins, alkaloids and flavonoids which were identified in the extract. •significant in the health care delivery system •justifies the use of the plant in the treatment of sores, boils, wounds and control of dysentery and diarrhea
Background of the Study
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Weight range of ratsPangsugat for equal size and depth; length of immunization
Methodology
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•E. hirta plants will be collected•The collected specimens will be confirmed•Dried leaves will be extracted with 95% ethanol
Plant Materials
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•Pure culture of S. aureus containing a concentration of 0.5 x 10^7 cfu/ml of innoculum, suspended in saline solution will be obtained
Preparation of inoculum
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•18 rats will be used for the investigation.•The subjects will be acclimatized and will be in good health•The rats will be shaved at the lumbosacral region.•A pair of 2 cm incisions will be done. One on each side.
Surgical wound creation, infection and treatment
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Irrigation was done with the use of plastic syringe with G-21 hypodermic needle attached
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After 3 days, the degree of skin and sub-cutis inflammation will be rated on a scale of 0 to 40 = normal 1 = minimal 2 = mild 3 = moderate 4 = severe neutrophilic
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ANOVA to differentiate colony count T-test (before and after) d ko na sulat kung para san
Statistical analysis
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Time Table
February march june july august
capsule presentation
proposal editing
thesis proposal presentation
sample collection & preparation
data collection
data analysis
final paper editing
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Budget proposal
Cost
Experimental subjects (albino rats, Staphylococcus aureus) = ESTIMATE ONLY
3000
Materials & instruments 700
Miscellaneous (transportation, printing, etc)
2000
Total