Effective Transportation and Translocation of Therapeutic

Molecules Using Suitably Functionalized Carbon Nanotubes

AISWARYA.N School of Chemical Sciences,

Mahatma Gandhi University, Kottayam, Kerala, India.

Introduction Deliverables Why carbon nanotubes? Functionalized CNTs as Innovative Tool. How the Drug Delivery…? Future Applications Concluding Remarks References

CONTENTS

IntroductionIn 1991, Iijima discovered carbon nanotubes (CNTs) that are elongated fullerenes where the walls of the tubes are hexagonal carbon and often capped at each end.

Carbon Nanotubes

Micro-crystals with high aspect ratios of 1000 or more, although their diameter is close to molecular dimensions

Single-Walled Carbon Nanotubes (SWCNTs).

Multi-Walled Carbon Nanotubes (MWCNTs)

Deliverables

Future aspects.

Importance of Carbon nanotubes as a ‘microshuttle’ for drug delivery.

The effect of functionalization for the therapeutic applications

Advantages and challenges in the field of drug delivery using the functionally modified carbon nanotubes.

Why Carbon Nanotubes…?

CNTs – the pitt version

Bionano materials such as liposomes

Nanotubes over nanospheres….?

Polymer or lipid based conjugate system.

A new entity with novel therapeutic or diagnostic properties

Functionalized CNTs as Innovative Tool

Solubility

Biocompatible natureNeed for Functionalization

........ . . .... . ... . . . .

X % X %

Functionalization of CNTs

Step 1 :

Step 2 :

Step 3:

Step 4:

sidewall tip

Molecular Structures of the Carbon Nanotube Conjugated

with Different Therapeutic Agents

Ultrathin transverse section of a HeLa cell treated with ammonium-functionalized CNT

Plasmid DNA delivery Cell internalisation

Antibiotic delivery Anticancer delivery

Impact of f-CNTs in immune system

Dendritic CellMacrophages T Lymphocyte

Two aspects of cell functionality studied Potential activation of immune cells

The capacity of immune cells that have previously encountered f-CNTs to respond to a physiological stimulus

Immune Cells- ‘True Soldiers’ of human body

Advantages of Using f-CNT as Vector Drug Delivery Offer multimodel options (Diagnosis, transport and targeted delivery to always) Specificity

Nano needle mechanism Solubility

Controlled drug release

Disadvantages Toxicity

Selectivity for functionalisation

How the Drug Delivery?

..

.

.

.......

Challenges Selection of the functionalizing agents. Translocation of the therapeutic molecule.

Biodistribution of f-CNTs.

Future Applications Microshuttle.

A new tool for the development of non-toxic drugs through cost effective routes.

Concluding Remarks

●The functionalization of CNT is still a subject of intense research, which generates continuous advances as well as novel materials.

●Offers “rediscovering” of old drug molecules and also offer new discovery and design options for new molecules.

●Anticancer and antibiotic carbon nanotube conjugates as well as antigen- presenting conjugates for the potential fabrication of vaccines and plasmid DNA complexes for construction of gene therapeutics.

References1.H.J.BWu,Dumortier,S.Lacotten,G.Pastorin,R.Marega,W.D.Bonifazi,J.Br

iad,M.Prato,S.Muller and A. Bianco,Nano letters,2006,6(7),1522.

2.A. Bianco,K.Kostarelos and M.Prato,Current Opinion in Chemical Biology,2005,9,674.3. CR Martin, P Kohli : The emerging field of nanotube biotechnology. Nat Rev Drug Discov 2003, 2:294. A.Bianco, K.Kostarelos, C.D Partidos, M.Prato. Biomedical applications of functionalised carbon nanotubes. Chem. Commun. 2005, 571.5. Y. Lin ,S.Taylor, H.Li , SKA.Fernando ,L. Qu ,W. Wang , et al. Advances towards bioapplications of carbon nanotubes. J Mater Chem 2004;14(4):527.

Sincere Thanks ToDr.P.K.RadhakrishnanDr.Sabu ThomasDr.Suresh MathewSchool of Chemical SciencesIrshad.K

Muhammed Ashik U P

Special Thanks ToChemistry Alumni AssociationStaff and Students of M.E.S Ponnani College