electrochemical methods prof. jin ho bangbk21bionano.hanyang.ac.kr/run/data/board/37... · 2020....
TRANSCRIPT
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Electrochemical Methods
Prof. Jin Ho Bang
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Biosensors
Nature Biotechnology 2019, 37, 389–406.
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Basic Principle of Biosensors
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Glucose Amperometric Biosensor
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Potential step methods
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Potential step methods
Chronoamperometry
Chronocoulometry
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Cu2+ + 2e → Cu0 (electrochemical reduction)
Fe2+ → Fe3+ + e (electrochemical oxidation)
Anode: electrochemical oxidation
Cathode: electrochemical reduction
Electrochemical reactions
Oxidation: electron moves to electrode.
Reduction: electron moves from electrode.
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• Electron tunneling: an electron can penetrate a potentialbarrier higher than the kinetic energy of the electron.
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Heterogeneous charge transfer
Cu2+ + 2e → Cu
Heterogeneous charge transfer reaction;
Electron tunneling at electrode/solution interface
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Electrode potential is an expression of electron energy
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Current is an expression of reaction rate
Current (i) ∝ Reaction rate
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Pathway of a general electrode reaction
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Potential step methods
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Potential step methods
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Cottrell equation
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Laplace transformation (partial to ordinary)
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CO(x,t) by Laplace transformation
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Error function
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Simulation of CO(x,t)
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Limitation of potential step method
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Limitation of potential step method
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Equivalent circuit for 3-electrode cell
Ref
Ctr
RW
Ru
Cdl
etrue
eref
WkWk
Cdl
etrue
eref
RW
Ru
Rct
Ref
Ctr
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Limitation of potential step method
Time constant = RuCdl
etrue
erefe
0 Time
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Limitation of potential step method
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1. Three-electrode configuration
2. Electrode area; small
- CO*, CR*; constant
3. Mass transfer; no migration (electrolytes)
- Diffusion
- No convection; no stirring
4. Spherical diffusion
Ultra microelectrode
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Planar vs. Planar ultra microelectrode
Planar microelectrode Planar ultra microelectrode
• The flux per unit area in spherical diffusion is greater than that in linear diffusion.
• Current decrease by decreased concentration gradient is compensated by the
increased flux. As a result, current density per unit area is greater in UME.
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Planar ultra microelectrode
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Planar ultra microelectrode
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Planar ultra microelectrode
• The used of UME alleviates issues associated with charging time and rising time.
• It also allows to measure steady current within a short time.
• From the steady current measured, parameters related to diffusion can be
determined.
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과제:
• 블랙보드에업로드된논문중 관심부분을골라요약 정리하고, 블랙보드를
통해온라인제출
• 제출기한: 9월 20일까지