embryo culture beyond implantation: past, present and futurecme-utilities.com/mailshotcme/material...
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Marta Shahbazi PhD Magdalena Zernicka-Goetz group
University of Cambridge, UK
COGEN Meeting 3rd November 2018, Paris, France
Disclosure information: nothing to declare
Embryo culture beyond implantation: past, present and future
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Human pregnancy loss: When? Why?
The pregnancy loss iceberg (modified from Macklon et al (2002) Human Reproduction)
Pre-implantation failure 30%
Conception
Early post-implantation failure
30%
PRE-CLINICAL LOSS
CLINICAL LOSS
Miscarriage 10%
Live births 30%
Day 0
Day 7
Day 14
Fetal abnormalities are present in 85% of early pregnancy losses
Larsen et al (2013) BMC Medicine
Chromosomal abnormalities are
detected in 50% of early pregnancy losses
den Berge et al (2012) Biochim Biophy Acta
The embryo or the endometrium?
What are the morphogenetic transformations
that a human embryo undergoes
at implantation?
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Human embryo development at implantation: methods to explore the black box of development
Co-culture of human embryos with endometrial cells: Uterine- embryo crosstalk and attachment. Morphogenesis? Lindenberg et al, Human Reprod (1986); Weimar et al, Reprod Biomed Online (2013)
Analysis of in vivo developing embryos (Carnegie Institution): A collection of more than 10,000 human embryos
Day 1 Day 6 Day 9 Day 16
Hertig et al, Am J Anat (1956)
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Human embryo development at implantation
Hallmarks of human post-implantation embryos
Lineage specification: - Embryonic tissue: epiblast - Extra-embryonic tissues: hypoblast and trophoblast Morphogenetic transformations: - Epiblast: amniotic cavity - Hypoblast: yolk sac - Trophoblast: cytotrophoblast and
syncytiotrophoblast
Day
9
Hertig et al (1956) Am J Anat
Day
6
Implantation Day 7-8
A global morphogenetic transformation takes place at the time of implantation
Can we develop a system to culture human embryos beyond day 7 in vitro?
What are the self-organizing capabilities
of human embryos?
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In vitro culture of mouse embryos beyond implantation
Pre-implantation blastocyst
Post-implantation mouse embryo
Morris et al (2012) Nat Comm; Bedzhov et al (2014) Nat Prot; Bedzhov et al (2014) Cell
In vitro culture
Shahbazi et al (2016) Nat Cell Biol; Deglincerti et al (2016) Nature
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Early post-implantation morphogenesis of the human embryo
Epiblast Hypoblast Trophectoderm
Shahbazi et al (2018) Nat Cell Biol
Day 7-8 Day 8-9 Day 9-10 Day 10-11
Embryo growth
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DAY 7-8: Segregation of epiblast and hypoblast
Epiblast (OCT4) Hypoblast (GATA6)
Day 9-10
OCT4/GATA6/DAPI
Inner cell mass
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Day
8-9
h
um
an e
mb
ryo
DAY 8-9: Amniotic cavity formation
OCT4/F-actin aPKC: apical polarity complex
Hu
man
ES
Cs
(3D
mat
rix
)
OCT4 Amniotic cavity
Day 9-10
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Mouse versus human post-implantation development
OCT4/F-actin/DAPI
E8-9 human embryo E4.75 mouse embryo
Development at implantation
Rosette
E10-11 human embryo E5.5 mouse embryo
Development at early post-implantation
OCT4/F-actin/DAPI
Cylinder Disc
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DAY 10-11: Differentiation of extra-embryonic tissues
Cytokeratin 7: Pan-trophoblast marker
Trophoblast
Differentiation into cytotrophoblast and syncytiotrophoblast
F-actin/DAPI
Hypoblast
DAPI/GATA6/aPKC/OCT4
Formation of the prospective yolk sac
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DAY 10-11: Differentiation of extra-embryonic tissues D
ay 8
D
ay 1
2
Day
10
D
ay 1
2
hCG: expressed specifically by syncytiotrophoblast cells
Deglincerti et al (2016) Nature
GATA3: expressed specifically by cytotrophoblast cells
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Culture of human embryos beyond implantation
Shahbazi et al (2016) Nat Cell Biol Deglincerti et al (2016) Nature
What have we learned?
Human embryos self-organize in vitro in the absence of maternal tissues
In both mouse and human embryos the epiblast undergoes a process of epithelial tissue formation through a rosette-intermediate step
What can we do with this system?
Explore the mechanisms behind early pregnancy loss: - Mechanisms of embryonic morphogenesis - Aneuploidy
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What directs amniotic cavity formation in the human embryo?
Alterations in amniotic cavitation may contribute to pregnancy loss at implantation
WT embryo β1 integrin KO embryo
Stephens et al (1995) Genes&Dev
In mouse embryos lack of amniotic cavity
formation leads to embryo loss at implantation
Epiblast Hypoblast Trophectoderm
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Epiblast morphogenesis and amniotic cavity formation
β1-INTEGRIN Epiblast (OCT4)
Basal: extracellular matrix
Apical: lumen Integrins: critical for attachment to the substrate and to provide directionality to the
cells (inside versus outside)
Day 9 human embryo
β1-INTEGRIN/OCT4/F-ACTIN Unpublished results
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Lineage specification
Differentiation
Pluripotency (Oct4+)
Hypothesis: loss of naïve pluripotency is necessary for amniotic cavity formation at the time of embryo implantation
Nakamura et al (2016) Nature; Smith et al (2017) Development
Primed (lineage-biased)
What directs amniotic cavity formation in the human embryo?
Naïve (unrestricted)
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Pluripotent state transitions
Amniotic cavity formation
??
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Epiblast morphogenesis and naïve pluripotency exit
KLF17/GATA6/PODXL/DAPI
Blastocyst (E6)
Addition of 5i (MEKi, GSK3i, RAFi, SRCi, ROCKi) + human LIF
+ Activin-A + bFGF2 blocks human ESCs in the naïve state
(Theunissen et al, 2014, Cell Stem Cell)
KLF17: naïve pluripotent epiblast GATA6: hypoblast PODXL: amniotic cavity
Post-implantation embryo (E9-10)
IVC
co
ntr
ol
KLF17/PODXL/DAPI
N=4/9
IVC
+5i
/LA
F
N=0/11
Shahbazi et al (2017) Nature
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Naïve human ESCs fail to form a cavity in 3D culture N
aïve
P
rim
ed
F-actin/PODXL/DAPI
3/30
26/30
3D human ESC cultures 3D mouse ESC cultures
F-actin/PODXL/DAPI
Naï
ve
Pri
me
d
1/40
34/40
Co
ntr
ol s
iRN
A
Oct
4 s
iRN
A
Par6/F-actin/Oct4
32/39
1/32
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Pluripotent state transitions coordinate epiblast morphogenesis
Shahbazi et al (2017) Nature
Integrins
Integrins
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What are the molecular mechanisms of early post-implantation failure?
The pregnancy loss iceberg (modified from Macklon et al (2002) Human Reproduction)
Pre-implantation failure 30%
Conception
Early post-implantation failure
30%
PRE-CLINICAL LOSS
CLINICAL LOSS
Miscarriage 10%
Live births 30%
Day 0
Day 7
Day 14
Amniotic cavity formation at implantation is fundamental for developmental progression.
This process is directed by integrins and a pluripotency state transition.
Mutations in any of these machineries would lead to early post-implantation failure
Aneuploidy: How do specific chromosomal alterations
affect amniotic cavity formation and developmental
progression?
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In vitro culture of monosomic and trisomic human embryos
Trisomy 16 Monosomy 21
Oct4/Gata6/F-actin/DAPI
Day 9 Day 9
Do embryos with different chromosomal abnormalities show different developmental potential in vitro ?
Extended culture of monosomic and trisomic embryos (based on the PGT-A result)
Unpublished results
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In vitro culture of monosomic and trisomic human embryos
8
3
8
23
16
2 **
p=0.09
*
Monosomic embryos die/arrest in culture significantly more than trisomic embryos
The monosomic embryos that do develop up to day 9 are slightly smaller and have an altered ICM/TE ratio compared to trisomic embryos
Our in vitro culture method is a bona fide platform to assess developmental competency
Unpublished results
?
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Guys Hospital, King’s College London, UK
Yakoub Khalaf Dusko Ilic
University of Bordeaux Gaelle Recher
CARE Fertility Clinic, UK Simon Fishel
Alison Campbell
Magdalena Zernicka-Goetz group
Human embryo team
Magda Matteo
CRICK institute, UK Kathy Niakan
IVI-RMA, US Emre Seli
Richard Scott Xin Tao
Tianren Wang
Past members: Sanna, Aga and Ania
Sanger Institute Thierry Voet Sam Behjati Tim Coorens Roser Vento
CTR, University of Cambridge
Graham Burton
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