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Emerald Ash Borer Regional Meeting May 17, 2017 EAB Regional Meeting | www.mda.state.mn.us/eab

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Page 1: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Emerald Ash Borer Regional Meeting

May 17, 2017

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 2: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Agenda

Time Topic

9:00-9:20 EAB Biology

9:20-9:40 EAB Regional Status

9:40-10:00Strategic Removal Of Host Trees In Isolated, Satellite Infestations Of EAB Can Reduce Population Growth

10:00-10:05 BREAK

10:05-10:20 Biocontrol & Biosurveillance

10:20-10:50 Using Monitoring Data to Optimize EAB Management

10:50-11:00 UMN Past & Present Projects

11:00-11:05 BREAK

11:05-11:25 EAB Cold Tolerance

11:00-noon Participant Discussion

Page 3: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Biology

EAB Regional Meeting | www.mda.state.mn.us/eab

Angie Ambourn | Entomologist

Page 4: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Life Cycle

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 5: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Life Cycle

Jan

Feb

Mar

Apr

May

Jun

Jul

Aug

Sep

Oct

Nov

Dec

Immature (under bark)

Adult (free living)

Page 6: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

How Does EAB Kill Trees?

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 7: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Host Trees

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 8: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Ash Tree ID

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 9: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Host Trees

EAB Regional Meeting | www.mda.state.mn.us/eab

David Cappaert

Black ash

Green ash

White ash

Blue ash

Manchurian ash

Susceptibility

low

high

Green ash

(throughout MN)

Black

ash

White

ash

Black Ash Cultivars• Fallgold

Green Ash Cultivars• Bergeson• Cimmaron• Marshall• Patmore• Summit

White Ash Cultivars• Autumn Applause• Autumn Blaze• Autumn Purple• Baltimore• Greenspire• Northern Blaze• Rosehill• Skyline

Page 10: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Secondary Host Tree

• Olive Family - Oleaceae

• White Fringetree

Chionanthus virginicus

EAB Regional Meeting | www.mda.state.mn.us/eab

William M. Ciesla, Forest Health Management International, Bugwood.org

Page 11: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Fringetree Risk

• Not a preferred host but EAB can complete its life cycle

• Sold in nurseries as an ornamental shrub

EAB Regional Meeting | www.mda.state.mn.us/eab

Dow Gardens , Dow Gardens, Bugwood.org John Ruter, University of Georgia, Bugwood.org

Page 12: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Recognizing EAB

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 13: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Confirmation

EAB Regional Meeting | www.mda.state.mn.us/eab

“S” shaped

galleries

EAB Larva

Page 14: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Confirmation

EAB Regional Meeting | www.mda.state.mn.us/eab

“D” shaped exit holes EAB Adult

1/8” width

Page 15: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 16: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Signs & Symptoms

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 17: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Symptom Progression

EAB Regional Meeting | www.mda.state.mn.us/eab

EAB Status SymptomsYears

Infested

Small larvae present None 1

Large larvae present / Emerging adults

Woodpecking possible 2

Increasing larvaenumbers

Woodpecking likelyBark splits possible

3

Canopy impacts visible 4

Dead trees present 5-6

Page 18: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Woodpecker Damage

• Mid / Top canopy

• Branches 3-6” in diameter

• Bark transitions to rough

• Bark blonding / flaking

• Dime sized woodpecker holes

• Light colored woodpecker holes

• Oval shaped

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 19: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Woodpecker Damage

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 20: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Bark Splits

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 21: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Native Damage

• Trunk

• Lower canopy

•Dead limbs

mda.state.mn.us/eab

Page 22: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Emerald Ash Borer Regional Meeting

May 17, 2017

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 23: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Regional Status

EAB Regional Meeting | www.mda.state.mn.us/eab

Jennifer Burington | EAB Community Liaison

Page 24: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Distribution

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 25: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Using Images

mda.state.mn.us/eab

Page 26: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Minnesota Distribution

• Generally infested area in green within red EAB quarantined counties

• Quarantined Counties

• Ash

• EAB

• Hardwood Firewood (<4 feet in length)

mda.state.mn.us/eab

Page 27: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Interactive Map

• mda.state.mn.us/eabstatus

• Closest known infested ash tree

Page 28: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Using Images

mda.state.mn.us/eab

August 2013• Superior, WI

October 2015• Park Point

June 2016• Thunder Bay, Ontario

September 2016• Duluth mainland

March 2017• Hartley Park

Page 29: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Using Images

mda.state.mn.us/eab

Page 30: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Slow Spread

• Cold weather

• Education

• Outreach

• Aggressive management

• Quarantines

• Sanitation

Page 31: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Management

mda.state.mn.us/eab

Page 32: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Treatment Plans

• Free permit for private treatment of public trees

• In-house treatments of public trees, $4.77/Diameter inch

• City contract rate extended to private residents, ~$6/D inch

• Treat trees to remove at a later date while waiting for diverse plantings to grow or funds become available

mda.state.mn.us/eab

Page 33: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Removal Plans

• Pre-EAB remove poor quality

• Remove woodpecked trees

• Remove private infested trees (Diseased Tree Ordinance)

• Remove all trees regardless of infestation

• Remove hazard trees

mda.state.mn.us/eab

Page 34: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Outreach Efforts

• City Water Bill Insert

• Tree Wraps

• Press Releases

• Postcard Mailings

• Don’t Move Firewood Poster

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 35: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

City A

• Remove poor quality trees

• Remove public infested trees

• Remove private infested trees within a specific distance of adjacent property

• Treat public mature trees in-house, park and boulevard

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 36: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

City B

• Remove poor quality trees

• Remove public infested trees

• No treatments

• No removal of private infested trees

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 37: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

City C

• Remove poor quality trees

• Remove public infested trees

• Remove private infested trees

• Treat mature public park trees, contract

• Treat private trees, offer contract rate

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 38: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Reporting

mda.state.mn.us/eab

Page 39: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Arrest the Pest

• Take pictures and notes

• Capture the insect or remove bark to see gallery/larvae

• Report

• GLEDN app (Great Lakes Early Detection Network)

• mda.state.mn.us/arrestthepest

[email protected]

• Call 888-545-6684 and leave a detailed message

mda.state.mn.us/eab

Page 40: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Emerald Ash Borer Regional Meeting

May 17, 2017

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 41: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Biocontrol & Biosurveillance

EAB Regional Meeting | www.mda.state.mn.us/eab

Jonathan Osthus| EAB BioControl Coordinator

Page 42: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Biological Control Agents

• Egg Parasitoid

• Oobius agrili

• Larval Parasitoid

• Tetrastichus planipennisi

• Larval Parasitoid

• Spathius galinae

Page 43: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Biological Control - Implementation

Over 449,000 parasitoid wasps released at 35 sites since September 2010

Biocontrol Agent 2010 2011 2012 2013 2014 2015 2016 All

Tetrastichus

planipennisi2,154 19,480 19,822 42,579 34,434 151,022 45,288 314,779

Oobius agrili 0 3,641 10,241 8,597 12,062 31,490 42,600 108,631

Spathius agrili 1,172 7,596 15,258 0 0 0 0 24,026

Spathius galinae 0 0 0 0 0 0 1,613 1,613

Totals 3,326 30,717 45,321 51,176 46,496 182,512 89,501 449,049

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 44: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Biological Control - Assessment

• Recovery Methods

• Tree Debarking

• Bark Sifting

• Yellow Pan Trapping

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 45: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

BioSurveillance

• Smoky winged beetle bandit wasp

• Cerceris fumipennis

• Citizen Engagement – Wasp Watchers

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 46: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Emerald Ash Borer Regional Meeting

May 17, 2017

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 47: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Using Monitoring Data to Optimize EAB Management

Mark Abrahamson, Assistant Director, Plant Protection Division

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 48: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB is Bad

EAB Regional meeting/https://www.mda.state.mn.us/emeraldashborer

Page 49: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Trees are Good!

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 50: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012
Page 51: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

1. Removed (before or after death)

2. Treated with insecticide

3. Die and fall apart

Fate of EAB Infested Trees

Page 52: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012
Page 53: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Comparing Detection Methods

Use of Monitoring to Inform Management

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 54: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

EAB Lifecyle

Trap Survey During

Summer

Branch Sampling

During Fall / Winter /

Spring

May 1

September 30

Visual Survey During Fall / Winter / Spring

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 55: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Lots of EAB

Moderate to low EAB

No EAB?

Page 56: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

ShoreviewCanopy Rating

2013

Page 57: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0

20

40

60

80

100

120

< 20% 20 - 40% 40 - 60% 60 - 80% > 80%

Fre

qu

en

cy

Canopy Rating

5 of 182 trees beyond

treatment (~3%)

EAB Regional meeting/https://www.mda.state.mn.us/emeraldashborer

Shoreview 2013

Page 58: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

GRB – King’s BluffCanopy Rating

2013

Page 59: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0

5

10

15

20

25

30

35

40

45

50

< 20% 20 - 40% 40 - 60% 60 - 80% > 80%

Fre

qu

en

cy

Canopy Rating

103 of 118 trees beyond

treatment (~87%)

GRB Kings Bluff 2013

Page 60: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0 25 50 75 100

% of trees per site with woodpecking damage

0

10

20

30

40

50

60

70

80A

vg

la

rva

l g

alle

rie

s/m

2/b

ran

ch

/sit

e

y = 1.28 + 0.09x

R2 = 0.9085

2013

0 25 50 75 100

% of trees per site with woodpecking damage

0

12

24

36

48

60

72

Av

g la

rva

l g

alle

rie

s/m

2/b

ran

ch

/sit

e

y = 0.52 + 0.08x

R2 = 0.9591

2014

Value of Monitoring-Woodpecking (Branch Sampling)

**

* From Flower, et al. 2013. The relationship between the emerald ash borer and ash tree decline… Analysis by Wilke and Aukema, University of Minnesota

Page 61: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0 25 50 75 100

% of trees per site with woodpecking damage

0

15

30

45

60

75

90

105A

vg

la

rva

l g

alle

rie

s/m

2/t

ree

/sit

e

y = 0.47 + 0.12x

R2 = 0.9191

0 25 50 75 100

% of trees per site with woodpecking damage

0

10

20

30

40

50

60

70

Av

g la

rva

l g

alle

rie

s/m

2/t

ree

/sit

e

y = -0.22 + 0.08x

R2 = 0.9923

*

*2013 2014

Value of Monitoring- Woodpecking (Whole Tree)

* From Flower, et al. 2013. The relationship between the emerald ash borer and ash tree decline… Analysis by Wilke and Aukema, University of Minnesota

Page 62: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0 25 50 75 100

Avg EAB trap catch per site

0

20

40

60

80

Av

g la

rva

l g

alle

rie

s/m

2/b

ran

ch

/sit

e

y = -1.55 + 16.65x

R2 = 0.9705

Value of Monitoring- Trapping

2014*

* From Flower, et al. 2013. The relationship between the emerald ash borer and ash tree decline…

Analysis by Wilke and Aukema, University of Minnesota

Page 63: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

0.0

1.0

2.0

3.0

4.0

5.0

6.0

Branch Trap Visual

Hours Per Positive Unit

Year 1

Year 2

Year 3

Value of Monitoring- Time Management

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 64: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Funding Sources and Partners

MDA – Mark Abrahamson, Angie Ambourn, Chris Mallet, Jennifer Burington, Jon Osthus, William Martin

U of M – Brian Aukema, Rob Venette, Aubree Wilke, Sam FahrnerEAB Regional Meeting | www.mda.state.mn.us/eab

Page 65: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Thank you!

Angie Ambourn

Angie. [email protected]

651-201-6073

EAB Regional Meeting | www.mda.state.mn.us/eab

Page 66: Emerald Ash Borer Regional Meeting...EAB Biological Control - Implementation Over 449,000 parasitoid wasps released at 35 sites since September 2010 Biocontrol Agent 2010 2011 2012

Thank you!

[email protected]

888-545-6684

EAB Regional Meeting | www.mda.state.mn.us/eab