Symme3D Biotech One is the world's first fully integrated, high precision

3D printer for biomass that features a state of the art temperature and

humidity controlled incubator.

Our product supports extensive prints using live stem cells and it features

twin HRV active filters with germicidal lamps for laminar air flow

processing. Its capabilities extend to extremely high precision movement

below 1 micrometer on all axis, as well as modular and multifunctional

pneumatic extruders for a wide range of bio compatible materials.

With conventional cell monitoring procedures, a culture vessel has to be

taken out of the incubator for microscope observation, where cells are

subjected to stressful environmental changes and vibration. Researchers

then have to spend additional time re-positioning the vessel to find the

same observation points. The integrated automatic micropipette

dispenser eliminates these problems by providing a stable environment

so that the cultures don't suffer while they are being imaged and allowing

for a complete trace of the same live cells, including stem cells.

Interchangeable Hybrid Extruder

Triple FDM Conventional extruder

Temperature: 100 to 350C

Nozzle: 200 to 400μm diameter for FDA approved thermoplas-

tic (PLA, PETG, Nylon680, PET, PC, SoftPLA)

Pneumatic dispenser: Hamilton dual PSD/4 pump

Accuracy ±1% @ 100% of stroke

Precision 0.05% @ > 100% stroke

Fluid Path Borosilicate glass, PTFE, CTFE, PFA

Bio dispenser:

Triple Syringes of up to 50ml of bioink and biocompatible

gells (Collagen, Gelatin, Fibrin, Hyaluronic Acid, Alginate)

Nozzle Size: 80 to 200um

Automatic micropipette dispenser system

8-Channel

20-300 µL

Ultra high positioning system (<1μm)

360 degree rotation range

Temperature control

from +5 up to +80C (substrate holder)

from +10 to 39C (incubation chamber)

Humidiier

Resonant frequency (KHz) FR=2.6+-0.5

Resonant impedance (ohm) Rr<=500

Range: 30-70%

Ultraviolet germicidal irradiation (UVGI)

UV Lamp 9W at 265nm wavelength

UV LED: 3W at 415nm wavelength

HEPA iltering system

H14 (99.995 <=0.3um D.O.P) HEPAFilter

Flow rate: 1900m^3/h

Heat recovery ventilation (HRV) technology

Laminar low hood

400W impeller drive

Airlow: 0.45m/s (90fpm)

Monitoring: internal display, CO2 and air quality

monitoring sensors

Air Quality ISO 14644-1, Class 3

Sterile Compounding USP 797

Filtration IEST-RP-CC034.1, IEST-RP-CC001.3,

IEST-RP-CC007.1, EN 1822

Electrical Safety UL-C-61010-1, CE Mark ROHS

Double walled polycarbonate door

UV-Ray Penetrability wave length 500nm(UV/VIS): 1.2%

Impact Strength: CNS11335 (Unbreakable)

Inlammability: UL 94V2 (Self-extinguishing)

Delection Temperature ASTM D648,18.6KG/cm²130-degree left side OR right side swing

Electronics removable drawer

Eight independent CPUsSoC: Broadcom BCM2837

CPU: 4× ARM Cortex-A53, 1.2GHz

GPU: Broadcom VideoCore IV

RAM: 1GB LPDDR2 (900 MHz)

Networking: 10/100 Ethernet, 2.4GHz 802.11n wireless

Bluetooth: Bluetooth 4.1 Classic, Bluetooth Low Energy

Storage: 32GB internal microSD, external USB

7 Inch touchscreen displayScreen Dimensions: 194mm x 110mm x 20mm

Viewable screen size: 155mm x 86mm

Screen Resolution 800 x 480 pixels

Triple disposable iltering system

Integrated toolhead 1000x

automatic focus microscope

Image Sensor: 0.3M HD CMOS Sensor (Digital 2M)

Focus Range: Automatic Focus from 0~200mm

Video Capture Resolution: 640x480

Still Image Capture Resolution: 1600x1200(2M Pixel),

1280x960(1.3M Pixel),800x600, 640x480

Frame Rate: Max. 30f/s Under 600 Lux Brightness

Flicker Control: 50Hz / 60Hz Option

Video Format: AVI

Snap Shot Format: JEPG

Magniication Ratio: 1000X

TYPICAL USE CASES:

3D Drug discovery

Complex in-vitro modelling of human diseases

Scafold based tissue engineering

APPLICATIONS TO STUDY & IDENTIFY BIOLOGICAL

PROCESSES:

Cell-cell interactions

Diferentiation

Response to stimuli

Diferentiation, Proliferation and morphology

In vivo relevance

Drug metabolism and expression (gene, proteins)

Environment and Extracellular matrix contact

MATERIAL CANDIDATES:

Cells, bioactives and signal molecules

Hydrogels and biopolymers

Polycaprolactone and thermopolymers

Calcium phosphates

Collagen, Hyaluronic acid, and gelatins

MODULARITY:

Selection of multiple dispensing technologies

(bioactive friendly ink-jet, extrusion, metering)

Cell friendly UV laser unit for hydrogel cross-linking

and photo-polymerization

Bioprinting in a controlled environment

(modular triple iltering system incubator with

UV germicidal lamps, hepa particle ilter and

laminar low hood)

Printing under physiological condition with

piezo-based printing heads

Smallest footprint on the market

Micrometer scale process repeatability

Speciications

Monitoring: internal display and air quality monitoring sensors

Air Quality ISO 14644-1, Class 3

Sterile Compounding USP 797

Filtration IEST-RP-CC034.1

IEST-RP-CC001.3

IEST-RP-CC007.1

EN 1822

Electrical Safety UL-C-61010-1

CE Mark ROHS

Physical dimensions: 480 x 480 x 750mm

Working range: 200 x 200 x 200mm

Precision: +- 0.06709μm

Triple disposable independent smart iltering systems featuring

Temperature control:

from +5 up to +80C (substrate holder)

from +10 to 39C (incubation chamber)

Humidiier:

Resonant frequency (KHz) FR=2.6+-0.5

Resonant impedance (ohm) Rr<=500

Range: 30-70%

Ultraviolet germicidal irradiation (UVGI)

UV Lamp 9W at 265nm wavelength

UV LED 3W at 415nm wavelength

APPLICATIONS TO STUDY & IDENTIFY BIOLOGICAL PROCESSES

Cell-cell interactions

Diferentiation

Response to stimuli

Diferentiation, Proliferation and morphology

In vivo relevance

Drug metabolism and expression (gene, proteins)

Environment and Extracellular matrix contact

Cells, bioactives and signal molecules

Hydrogels and biopolymers

Polycaprolactone and thermopolymers

Calcium phosphates

Collagen, Hyaluronic acid, and gelatins

MATERIAL CANDIDATES

Interchangeable Hybrid Extruder:

Triple FDM Conventional extruder,

Temperature: 100 to 350C

Nozzle: 200 to 400um diameter for FDA approved thermoplastic

(PLA, PETG, Nylon680, PET, PC, SoftPLA)

Pneumatic dispenser: Hamilton dual PSD/4 pump

Accuracy: ±1% @ 100% of stroke

Precision: 0.05% @ > 100% stroke

Fluid Path: Borosilicate glass, PTFE, CTFE, PFA or ceramic

Bio dispenser:

Triple Syringes of up to 50ml of bioink and biocompatible gells

(Collagen, Gelatin, Fibrin, Hyaluronic Acid, Alginate

Nozzle Size: 80 to 200μm

HEPA iltering system: H14 (99.995 <=0.3um D.O.P) HEPAFilter

Flow rate: 1900m^3/h

Laminar low hood: 400W impeller drive

Airlow: 0.45m/s (90fpm)