evaluation of cardiac injury and function. introduction chd, – the most important disease...
TRANSCRIPT
Introduction
• CHD,– The most important disease affecting the heart is
coronary heart disease• ACS,– CHD, can lead to an acute blockage of coronary
blood flow known as an acute coronary syndrome • MI,– ACS with frank necrosis of any amount of
myocardium is known as myocardial infarction
Background
• Ischemia – the lack of an adequate blood supply
• Atherosclerosis– a chronic process involving damage to
endothelium and the build-up of vessel occluding lesions called plaque.
• angina pectoris (exertional angina)– Once the diameter of a coronary artery is reduced
to less than 10-20% of its original size
Background
• Myocardial infarction (MI)– Complete blockage of blood flow• Irreversible ischemic damage
• Unstable angina (UA)– When the blockage is not complete– irreversible muscle damage may be avoided– experience severe angina, even at rest
Diagnosis of Cardiac diseases(Outline)
• Cardiac diseases discussed in this session – Coronary heart disease (CHD)– Heart failure (HF)
• Diagnosis of Cardiac diseases– Clinical (non laboratory) examination
• e.g, Clinical history, ECG, angiography, echocardiography
– Biochemical markers• Markers of Myocardial Damage• Risk factors associated with the development and
progression of CHD.
ECG;ElectroCardioGraphy
Markers of Myocardial Damage
• Cardiac enzymes– Transaminases (SGOT,SGPT)– Lactate dehydrogenase (LD) – Creatine kinase (CK)
• Improved cardiac specificity– with separation of isoenzymes.• LD1 is relatively abundant in cardiac muscle
– Flipped LD, where the normal finding LD2> LD1] is reversed.
• In normal heart, 15-20% of the CK is CK-MB• Changes in LD observable for a much longer
time than changes in CK.• The isoenzyme analyses were relatively
lengthy & tedious– Could only be performed about once per day
• CK-MB Mass Assay– Can be performed rapidly
Cardiac Troponin
• The most important laboratory test for cardiac diagnosis.
• TnT (tropomyosin-binding subunit, 37 kDa)• Tnl (inhibitory subunit, 24 kDa), • TnC (calcium-binding subunit, 18 kDa).
Cardiac Troponin
• Tnl has a cardiospecific form (cTnl)• TnT also has distinct forms in myocardium
(cTnT)– Also, Fetal skeletal muscle and diseased skeletal
muscle.• post-translational modifications cause detectable
differences
Cardiac Troponin
• Bound form– released slowly over the course of 1-2 weeks following
myocardial infarction.• Free form– time frame similar to that of CK-MB, with cTn reaching a
peak at about 24 h following MI• cTnT and cTnl are nearly absent from normal serum• Small elevations– pericarditis, myocarditis, pulmonary embolism, renal
failure, sepsis, and other critical illness
• The high sensitivity and specificity of cTn• Patients with ischemic symptoms who also
have elevations in cTn receive greater benefit from therapies with various antiplatelet and anti thrombotic agents
Myoglobin
• Lacking cardiac specificity,• more rapidly than other proteins • Within 2-3 h following onset of MI, earlier
than with troponin or other markers• myoglobin peaks about 6 h after MI and
returns to baseline after 24• half-life,approximately 4 hours, but is longer if
renal function is impaired.
Myoglobin
• Muscle mass and muscle activity – Higher in men
• increases with increasing age• Day-to-day variation is about 10-15%• offers fairly high clinical specificity (> 95%)
when patients with renal failure or suspected injury to skeletal muscle are excluded.
• Sensitivity can be enhanced– δ Mb
Other Markers
• Carbonic Anhydrase III(CA III)– present in skeletal but not cardiac muscle,• Negative cardiac marker.
• Creatine Kinase Isoforms– a high ratio of MB2/MB1 suggests that a recent
release of enzyme has occurred.– in the first 3-4 hours following symptom onset,
improved sensitivity for MI detection
Limitations
• cTn– is a highly heterogeneous analyte.– Reference ranges and decision points have been a
matter of some confusion
Diagnosis of ACS
• Biochemical markers play a secondary role in the initial management of patients with suspected ACS.
• The earliest decision-making, which should ideally take place within 10 min of the patient's arrival in the ED, is based on history, physical examination, and 12-lead ECG
• Likely to be negative at this early time.
ACS: acute coronary syndrome. ED: emergency department
• Choice of markers and time points– cTn (either I or T) is the preferred marker for
definitive diagnosis• Also for early diagnosis,– Myoglobin but possibly CK isoforms, at the 0 and
1- to 4-h time points.
• noninvasive indicator that reperfusion has occurred,– Myoglobin was found to perform better than CK-
MB or cTn• early reinfarction – CK-MB appears to be superior to cTn because of
its more rapid decline following MI– myoglobin is also useful.
• Diagnosis of MI following surgical procedures– cTn is clearly the marker of choice in this situation,
Markers of Coronary Risk
• Laboratory tests– Serum cholesterol• LDL• HDL, a negative risk factor • Triglyceride
• C-Reactive Protein (CRP)– In acute illness, cytokines, chiefly interleukin-6,
stimulate hepatic production of CRP
Markers of Coronary Risk
• the usefulness of the hsCRP test in individual patients has been controversial.– the variations in quantitative risk estimates,– the unclear role of CRP in pathogenesis of vascular
disease,– the lack of specific treatment for high CRP,
Markers of Coronary Risk
• epidemiology studies – confirm a correlation between moderate Hey levels and CHD.
• Homocysteine (Hey)– total homocysteine (tHcy):
• free sulfhydryl group, • as a disulfide (homocystine), • as a mixed disulfide,
– linked to a plasma protein via one of its cysteine residues.
• Excessive levels of circulating Hcy– Enzyme defect
• Cystathionine-β-synthase
Markers of Coronary Risk
• Clinical manifestations– thromboembolic disease, including CHD
• The basis of the damaging effects of Hcy is uncertain.– Oxidant stress and inhibition of transmethylation
reactions are likely• Measurment – Chromatographic – Enzymatic
Clinical algorithm for treating patients presenting to an emergency department with symptoms suggesting ACS.
Markers of Congestive Heart Failure
• Cardiac Natriuretic Peptides– brain natriuretic peptide (BNP) produced mainly in
the cardiac ventricle– Secretion enhanced by ventricular wall stretch and
volume overload, as occurs in HF– Circulating half-life is approximately 22 minutes.– (N-terminal fragment)N-BNP is considerably
longer (60-120 min)
Markers of Congestive Heart Failure
• Applications of BNP testing– The best-established application
• For diagnosing acutely ill patients presenting to emergency service with shortness of breath.
• At a decision point of 100 pg/mL, the BNP test had the following characteristics for diagnosis of HF:– Sensitivity 90%,– Specificity 76%, – Positive predictive value 79%,– Negative predictive value 89%
Markers of Congestive Heart Failure
• Other applications of BNP testing– Monitoring the course and treatment of patients
with HF– Risk stratification of patients with ACS– Monitoring disease severity in patients with
stable CHD– Screening for ventricular dysfunction– Testing for drug cardiotoxicity
Markers of Congestive Heart Failure
• The major limitation of BNP – Decision point • a wide range of values is observed in patients with and
without HF
– Patients with symptomatic HF especially when it is chronic and stable, can have 'normal' levels
• So,– the most appropriate use of the BNP test is as an
adjunctive test to rule out HF in the acute setting.