experiment number: 20006 - 04 p09: non-neoplastic ......experiment number: 20006 - 04 p09:...
TRANSCRIPT
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F1_M3NTP Study Number: C20006B
Lock Date: 03/08/2010
Cage Range: ALL
Date Range: ALL
Reasons For Removal: ALL
Removal Date Range: ALL
Treatment Groups: Include ALL
Study Gender: Both
TDMSE Version: 2.6.0.0_007
PWG Approval Date: NONE
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + +
Gallbladder + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + +AtrophyInflammation, SuppurativeLymphangiectasis
Liver + + + + + + + + + + + + + + + + + + + + + + + + +Basophilic Focus X XClear Cell Focus X X X XEosinophilic Focus X X X X X X X X X X X X X X X XFatty Change 2 2 2 2 1 2 1 2 2 2 1 2 2 1 2 2 1Mixed Cell FocusNecrosisNecrosis, Multifocal
Mesentery + + +
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 2
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
Fat, Necrosis 3 2 2
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + +Acinus, Atrophy
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell 1 1 1 2 1 2 1 1 1 1 1 2 1
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer 4 2 2Epithelium, Hyperplasia 3
Stomach, Glandular + + + + + + + + + + + + + + + + + + + + + + + + +ErosionInflammation, Chronic 1Mineralization 1 2
Tooth + + + + + + + + + + + + +Dysplasia 2 3 3 2 3 3 2 4 2 2 2 3 2
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + +Adventitia, Pulmonary Vein, Infiltration Cellular,
Polymorphonuclear1
Heart + + + + + + + + + + + + + + + + + + + + + + + + +Cardiomyopathy 1 1 3 1Inflammation, ChronicArtery, Inflammation, Chronic
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 3
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia 1
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia
Parathyroid Gland + + + + + + M M + + + + + + + + + + + + + + + + +
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + +Cyst 1
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + +Follicle, Cyst
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Coagulating Gland +
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + +
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 4
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
Cyst 2 1 2 2 2Inflammation, Suppurative
Prostate + + + + + + + + + + + + + + + + + + + + + + + + +HyperplasiaInflammation, Chronic
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + +Atrophy 3
Testes + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, GranulomatousInflammation, Chronic 2Mineralization 2Germinal Epithelium, Atrophy 1 1
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + +
Lymph Node
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + +
Lymph Node, Mesenteric + + + + + + + + + + + + + + + + + + M + + + + + +Hematopoietic Cell Proliferation 3
Spleen + + + + + + + + + + + + + + + + + + + + + + + + +Hematopoietic Cell Proliferation 2 1 2 2 2 1 2NecrosisLymphoid Follicle, Atrophy
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 5
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
Thymus + + + + + + + + + + + + + + + + + + + + + + + + +Atrophy 4 2 2 4 2 2 2 3 4 4 4 1 4 4 4 4 4 3 3 2 2 3
INTEGUMENTARY SYSTEM
Mammary Gland M M M M M M M M M M M + M M M M M M M M M M M M M
Skin + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer 3 3
MUSCULOSKELETAL SYSTEM
Bone + + + + + + + + + + + + + + + + + + + + + + + + +
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + +
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + +Alveolar Epithelium, Hyperplasia
Nose + + + + + + + + + + + + + + + + + + + + + + + + +Foreign Body XInflammation 1 2 1 1 1 1 1Polyp, Inflammatory 2Olfactory Epithelium, Accumulation, Hyaline
Droplet1
Olfactory Epithelium, Atrophy 3Olfactory Epithelium, Degeneration 1
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 6
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
Olfactory Epithelium, Respiratory Metaplasia 2 1 1 1 1 1 1 1 1Respiratory Epithelium, Accumulation, Hyaline
Droplet1 1 1 1 2 1 1
Respiratory Epithelium, Hyperplasia 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Trachea + + + + + + + + + + + + + + + + + + + + + + + + +
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + +Phthisis Bulbi XCornea, Inflammation, Chronic 1 1
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + +HyperplasiaInflammation, Suppurative
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + +Infarct 1 2Inflammation, Chronic 2Metaplasia, OsseousMineralization 1Nephropathy 1 2 1 2 2 2 1 1 1 1 3 1 1 1 1 2 4 1 2 1 2 1 1Papilla, NecrosisPelvis, Inflammation, SuppurativeRenal Tubule, CystRenal Tubule, PigmentationRenal Tubule, Vacuolization Cytoplasmic 2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 7
-
DAY ON TEST
B6C3F1 MICE MALE
0731
0673
0732
0732
0463
0732
0730
0546
0731
0730
0711
0644
0501
0532
0732
0732
0731
0732
0717
0731
0731
0731
0626
0469
0680
0 mg/kgANIMAL ID
00001
00002
00003
00004
00005
00006
00007
00008
00009
00010
00011
00012
00013
00014
00015
00016
00017
00018
00019
00020
00021
00022
00023
00024
00025
males(cont...)
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 8
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + + 50
Gallbladder + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + + 50Atrophy 3 1 3.0Inflammation, Suppurative 1 1 1.0Lymphangiectasis 2 1 2.0
Liver + + + + + + + + + + + + + + + + + + + + + + + + + 50Basophilic Focus 2Clear Cell Focus X X X 7Eosinophilic Focus X X X X X X X X X X X X X 29Fatty Change 1 2 2 2 2 1 2 2 2 2 2 1 29 1.7Mixed Cell Focus X 1Necrosis 2 4 2 3 2.7Necrosis, Multifocal 3 1 3.0
Mesentery + + + + 7
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 9
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
Fat, Necrosis 2 4 2.3
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + + 50Acinus, Atrophy 1 1 1.0
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 1 1 1 1 1 1 1 2 1 23 1.2
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + + 50Ulcer 3 2 3 6 2.7Epithelium, Hyperplasia 2 2 2.5
Stomach, Glandular + + + + + + + + + + + + + + + + + + + + + + + + + 50Erosion 2 1 2.0Inflammation, Chronic 1 1.0Mineralization 1 2 1 5 1.4
Tooth + + + + + + + + + + + + + + + + + 30Dysplasia 3 2 1 1 3 1 1 1 1 2 2 1 3 4 1 3 3 30 2.2
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + 49Adventitia, Pulmonary Vein, Infiltration Cellular,
Polymorphonuclear 1 1.0
Heart + + + + + + + + + + + + + + + + + + + + + + + + + 50Cardiomyopathy 1 1 1 1 1 1 1 11 1.2Inflammation, Chronic 1 1 1.0Artery, Inflammation, Chronic 2 1 2.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 10
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1.0
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1 2 1.0
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1 1.0
Parathyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + + 48
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Cyst 1 2 1.0
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Follicle, Cyst X 1
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Coagulating Gland 1
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 1 1.0
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 11
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
Cyst 2 2 2 2 3 2 2 2 2 14 2.0Inflammation, Suppurative 2 2 2 2.0
Prostate + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 2 2 2 2.0Inflammation, Chronic 2 1 2.0
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + + 50Atrophy 1 3.0
Testes + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Granulomatous 2 1 2.0Inflammation, Chronic 1 2.0Mineralization 1 2.0Germinal Epithelium, Atrophy 2 1.0
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + + 50
Lymph Node + + 2
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + + 50
Lymph Node, Mesenteric + + + + + + + M + + + + + + + + + + + + + + + + + 48Hematopoietic Cell Proliferation 1 3.0
Spleen + + + + + + + + + + + + + + + + + + + + + + + + + 50Hematopoietic Cell Proliferation 3 2 3 3 2 2 1 1 2 16 1.9Necrosis 3 1 3.0Lymphoid Follicle, Atrophy 3 3 2 3.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 12
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
Thymus + + + + + + + + + + + + + + + + + + + M + + + + + 49Atrophy 4 3 4 4 4 4 3 3 1 1 4 3 2 4 3 3 3 4 3 4 3 4 44 3.1
INTEGUMENTARY SYSTEM
Mammary Gland M M M M M M M + M M M M M M M M M M M M M M M M M 2
Skin + + + + + + + + + + + + + + + + + + + + + + + + + 50Ulcer 3 3 3.0
MUSCULOSKELETAL SYSTEM
Bone + + + + + + + + + + + + + + + + + + + + + + + + + 50
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + + 50
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + + 50Alveolar Epithelium, Hyperplasia 3 1 1 3 1.7
Nose + + + + + + + + + + + + + + + + + + + + + + + + + 50Foreign Body X X 3Inflammation 2 1 1 1 1 1 1 1 15 1.1Polyp, Inflammatory 1 2.0Olfactory Epithelium, Accumulation, Hyaline
Droplet1 1 1 4 1.0
Olfactory Epithelium, Atrophy 2 1 3 2.0Olfactory Epithelium, Degeneration 1 1.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 13
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
Olfactory Epithelium, Respiratory Metaplasia 1 1 1 1 1 14 1.1Respiratory Epithelium, Accumulation, Hyaline
Droplet1 1 1 2 1 1 1 2 1 1 1 18 1.2
Respiratory Epithelium, Hyperplasia 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 35 1.0
Trachea + + + + + + + + + + + + + + + + + + + + + + + + + 50
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + + 50Phthisis Bulbi 1Cornea, Inflammation, Chronic 2 1.0
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1 1.0Inflammation, Suppurative 3 1 3.0
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + + 50Infarct 2 3 1.7Inflammation, Chronic 1 2.0Metaplasia, Osseous 1 1 1.0Mineralization 2 2 2 4 1.8Nephropathy 2 1 1 1 1 1 4 1 2 1 1 1 1 3 1 1 2 1 3 42 1.5Papilla, Necrosis 2 1 2.0Pelvis, Inflammation, Suppurative 3 1 2 2.0Renal Tubule, Cyst 1 1 1.0Renal Tubule, Pigmentation 2 1 2.0Renal Tubule, Vacuolization Cytoplasmic 1 2.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 14
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0732
0525
0728
0619
0629
0730
0589
0732
0731
0610
0354
0730
0483
0732
0519
0465
0731
0731
0731
0730
0732
0723
0662
0697
0 mg/kgANIMAL ID
00026
00027
00028
00029
00030
00031
00032
00033
00034
00035
00036
00037
00038
00039
00040
00041
00042
00043
00044
00045
00046
00047
00048
00049
00050 * TOTALS
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Chronic 3 1 3.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 15
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + +
Gallbladder + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer 3
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + +Peyer's Patch, Hyperplasia 2
Liver + + + + + + + + + + + + + + + + + + + + + + + + +Basophilic Focus X X XClear Cell Focus X X X X X XEosinophilic Focus X X X X X X X X X X X X X X X X XFatty Change 2 2 1 3 1 1 2 1 2 1 1 1 1Hepatodiaphragmatic Nodule XMixed Cell Focus X X XNecrosis 3
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 16
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Mesentery + +
Oral Mucosa
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + +Duct, Cyst
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic 1Ulcer 3 2 3 2 2Epithelium, Hyperplasia 3 3
Stomach, Glandular + A + + + + + + + + + + + + + + + + + + A + + + +Inflammation, Chronic 1Mineralization 2Pigmentation 1Epithelium, Hyperplasia 1
Tooth + + + + + + + + + + + + + + + + +Dysplasia 2 4 1 3 2 3 4 3 3 1 4 3 4 3 3 1 4
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + +Aorta, Mineralization 2
Heart + + + + + + + + + + + + + + + + + + + + + + + + +Cardiomyopathy 2 1 1 1
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 17
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Artery, Mineralization 2Myocardium, Mineralization 2 2 3
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + +Hypertrophy 2 2 1Vacuolization Cytoplasmic
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + +
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + +
Parathyroid Gland M + + + M + + + + + + + + M + + + + + + + + + + +
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + +CystPars Intermedia, Hyperplasia 1
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + +
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell 1 1 2Inflammation, Chronic
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 18
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + +Cyst 2 1 2 2 2 2Inflammation, Granulomatous
Prostate + + + + + + + + + + + + + + + + + + + + + + + + +
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + +
Testes + + + + + + + + + + + + + + + + + + + + + + + + +
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + +
Lymph Node + +Pancreatic, Necrosis 4
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia, Lymphoid
Lymph Node, Mesenteric + + + + + + + + + + + + + + + + M + + + + M + + +
Spleen + + + + + + + + + + + + + + + + + + + + + + + + +Hematopoietic Cell Proliferation 1 2 2 3 1 3 2 3
Thymus + + + + + + M + + + + + + + + + + + M + M + + + +Atrophy 4 1 2 2 1 3 2 3 4 3 2 4 4 4 4 4 3 1 2 3Ectopic Parathyroid Gland 1
INTEGUMENTARY SYSTEM
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 19
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Mammary Gland M M M M M M M M M M M M M M M M M M M M M M M M M
Skin + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer
MUSCULOSKELETAL SYSTEM
Bone + + + + + + + + + + + + + + + + + + + + + + + + +
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + +Meninges, Inflammation, Chronic 1
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Histiocyte 3 2Inflammation, ChronicPigmentation, Hemosiderin 2Alveolar Epithelium, Hyperplasia 1
Nose + + + + + + + + + + + + + + + + + + + + + + + + +Foreign Body XInflammation 1 1 1 2 1 1 2Polyp, Inflammatory 2 1Nerve, Olfactory Epithelium, AtrophyOlfactory Epithelium, Accumulation, Hyaline
Droplet1 1 2
Olfactory Epithelium, Atrophy 1 2 2Olfactory Epithelium, Degeneration 1
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 20
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Olfactory Epithelium, Respiratory Metaplasia 1 2 1 2 1 1 1 2 1Respiratory Epithelium, Accumulation, Hyaline
Droplet1 1 1 1 1 2 1 1 1 1 1 1 1 1 3 1 1 1
Respiratory Epithelium, Hyperplasia 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 2 1
Trachea + + + + + + + + + + + + + + + + + + + + + + + + +
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + +Phthisis BulbiAnterior Chamber, Inflammation, Suppurative 3Cornea, Inflammation, SuppurativeCornea, Inflammation, Chronic 2
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia 1Inflammation, Suppurative 3Inflammation, Chronic
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + +Infarct 3 2Inflammation, Acute 3 2Metaplasia, Osseous 1Mineralization 2 2 1 2Nephropathy 3 1 1 2 1 1 1 1 1 1 2 1 1 1 1 1 1 4 2 1 1 1 1 1 2Thrombosis XPelvis, Inflammation, Suppurative 2Renal Tubule, Cyst 2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 21
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0582
0673
0731
0682
0731
0730
0694
0573
0732
0631
0732
0701
0730
0716
0630
0692
0658
0730
0682
0714
0731
0731
0732
0732
62.5 mg/kgANIMAL ID
00051
00052
00053
00054
00055
00056
00057
00058
00059
00060
00061
00062
00063
00064
00065
00066
00067
00068
00069
00070
00071
00072
00073
00074
00075
males(cont...)
Renal Tubule, Pigmentation
Urethra +Inflammation, Acute 3
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + +Hemorrhage 2Inflammation, Chronic
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 22
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + + 50
Gallbladder + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + + 50Ulcer 1 3.0
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Chronic 3 1 3.0
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + + 50Peyer's Patch, Hyperplasia 1 2.0
Liver + + + + + + + + + + + + + + + + + + + + + + + + + 50Basophilic Focus X 4Clear Cell Focus X X X X X X X X X X X 17Eosinophilic Focus X X X X X X X X X X X X X X X X X X 35Fatty Change 1 1 2 1 1 2 1 2 1 2 2 1 2 2 27 1.5Hepatodiaphragmatic Nodule 1Mixed Cell Focus X X X 6Necrosis 1 2 2.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 23
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Mesentery + 3
Oral Mucosa + 1
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + + 50Duct, Cyst 2 1 2.0
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 30 1.0
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Chronic 1 1.0Ulcer 5 2.4Epithelium, Hyperplasia 2 3.0
Stomach, Glandular + + + A + + + + + + + + + + + + + + + + + + + + + 47Inflammation, Chronic 1 1.0Mineralization 1 2 1.5Pigmentation 1 1.0Epithelium, Hyperplasia 1 1.0
Tooth + + + + + + + + + + + + + + + + + + + + 37Dysplasia 4 3 1 3 1 3 3 1 2 4 1 3 3 1 3 3 3 3 2 36 2.6
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + + 50Aorta, Mineralization 1 2.0
Heart + + + + + + + + + + + + + + + + + + + + + + + + + 50Cardiomyopathy 1 1 1 7 1.1
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 24
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Artery, Mineralization 1 2.0Myocardium, Mineralization 3 2.3
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + + 50Hypertrophy 1 4 1.5Vacuolization Cytoplasmic 1 1 2 1.0
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + + 50
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + + 50
Parathyroid Gland + + + + M + + M + + + + + + + + M + + + + + + + + 44
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Cyst 1 1 1.0Pars Intermedia, Hyperplasia 1 1.0
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 1 1 6 1.2Inflammation, Chronic 3 2 2 2.5
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 25
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Cyst 2 2 2 2 10 1.9Inflammation, Granulomatous 1 2 2 1.5
Prostate + + + + + + + + + + + + + + + + + + + + + + + + + 50
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + + 50
Testes + + + + + + + + + + + + + + + + + + + + + + + + + 50
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + + 50
Lymph Node 2Pancreatic, Necrosis 1 4.0
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia, Lymphoid 4 1 4.0
Lymph Node, Mesenteric + + + + + + + + + + + + + + + + + + + + + + + + + 48
Spleen + + + + + + + + + + + + + + + + + + + + + + + + + 50Hematopoietic Cell Proliferation 4 3 2 2 2 1 3 2 16 2.3
Thymus + + + + + + + + + + + + + + + + + + + + + + + + + 47Atrophy 2 3 2 1 1 4 3 3 1 2 3 3 4 1 3 4 3 3 2 2 2 2 4 3 44 2.7Ectopic Parathyroid Gland 1 1.0
INTEGUMENTARY SYSTEM
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 26
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Mammary Gland M M M M M M M M M M M M M M M M M M M M M M M M M 0
Skin + + + + + + + + + + + + + + + + + + + + + + + + + 50Ulcer 3 3 2 3.0
MUSCULOSKELETAL SYSTEM
Bone + + + + + + + + + + + + + + + + + + + + + + + + + 50
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + + 50Meninges, Inflammation, Chronic 1 1.0
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Histiocyte 2 2.5Inflammation, Chronic 2 1 2.0Pigmentation, Hemosiderin 1 2.0Alveolar Epithelium, Hyperplasia 1 1 1 4 1.0
Nose + + + + + + + + + + + + + + + + + + + + + + + + + 50Foreign Body 1Inflammation 1 1 1 1 11 1.2Polyp, Inflammatory 2 1.5Nerve, Olfactory Epithelium, Atrophy 2 1 2.0Olfactory Epithelium, Accumulation, Hyaline
Droplet1 4 1.3
Olfactory Epithelium, Atrophy 2 1 5 1.6Olfactory Epithelium, Degeneration 1 1.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 27
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Olfactory Epithelium, Respiratory Metaplasia 1 1 2 1 1 14 1.3Respiratory Epithelium, Accumulation, Hyaline
Droplet1 1 1 1 1 1 1 2 2 1 1 1 1 1 1 1 34 1.1
Respiratory Epithelium, Hyperplasia 2 1 1 1 1 1 1 1 1 1 1 2 2 1 1 1 2 1 1 1 1 1 40 1.2
Trachea + + + + + + + + + + + + + + + + + + + + + + + + + 50
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + + 50Phthisis Bulbi X 1Anterior Chamber, Inflammation, Suppurative 1 3.0Cornea, Inflammation, Suppurative 3 1 3.0Cornea, Inflammation, Chronic 1 1 3 1.3
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 2 3 3 4 2.3Inflammation, Suppurative 1 3.0Inflammation, Chronic 4 1 4.0
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + + 50Infarct 2 2.5Inflammation, Acute 2 2.5Metaplasia, Osseous 1 1.0Mineralization 1 1 1 1 8 1.4Nephropathy 2 2 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 2 2 1 1 1 1 1 1 50 1.3Thrombosis 1Pelvis, Inflammation, Suppurative 3 2 2.5Renal Tubule, Cyst 1 2 3 1.7
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 28
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0732
0709
0732
0710
0732
0732
0731
0732
0732
0731
0731
0604
0732
0711
0381
0732
0731
0731
0732
0732
0730
0616
0730
62.5 mg/kgANIMAL ID
00076
00077
00078
00079
00080
00081
00082
00083
00084
00085
00086
00087
00088
00089
00090
00091
00092
00093
00094
00095
00096
00097
00098
00099
00100 * TOTALS
Renal Tubule, Pigmentation 2 1 2.0
Urethra 1Inflammation, Acute 1 3.0
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + + 50Hemorrhage 1 2.0Inflammation, Chronic 1 1 2 1.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 29
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + +Mineralization 2
Gallbladder M + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + +Peyer's Patch, Hyperplasia 2
Liver + + + + + + + + + + + + + + + + + + + + + + + + +Basophilic FocusClear Cell Focus X X X X X X X X X XEosinophilic Focus X X X X X X X X X X X X X X X X XFatty Change 2 1 1 2 1 1 2 2 2 2Mixed Cell Focus X X X XNecrosis
Mesentery + +Fat, Necrosis 1 2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 30
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + +Acinus, Atrophy
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell 1 1 1 1 1 1 1 1 1 1 1 2 1 1Duct, Sublingual Gland, Hyperplasia 1
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic 2Ulcer 2 2 1Epithelium, Hyperplasia
Stomach, Glandular + A + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic 1 1 1 1 1 1 1 1 1 1 1Pigmentation 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1Epithelium, Hyperplasia 1 1 1 2 1 2 1 2 1 1 1 1 1
Tooth + + + + + + + + + + + + + +Dysplasia 2 3 3 4 4 3 4 3 4 2 1 1 1 3
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + +
Heart + + + + + + + + + + + + + + + + + + + + + + + + +Cardiomyopathy 1 1 1 1
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + +
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 31
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
Hypertrophy
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia 1
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia 3
Parathyroid Gland + + + + + + + + + + + + + M + + + + + + + + + + +
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + +Cyst
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + +
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + +Infiltration Cellular, Mononuclear Cell 1 1
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + +Cyst 2 2 2
Prostate + + + + + + + + + + + + + + + + + + + + + + + + +HyperplasiaInflammation, Suppurative 2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 32
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + +
Testes + + + + + + + + + + + + + + + + + + + + + + + + +Germinal Epithelium, Atrophy 1 1
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + +
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + +
Lymph Node, Mesenteric + + + + + + + + + + + + + + + + + + + + + + + + +
Spleen + + + + + + + + + + + + + + + + + + + + + + + + +Accessory SpleenHematopoietic Cell Proliferation 2 2 2 3 3 2 3 1 1 2 1 3
Thymus + + + + + M + + + + + + + + + + + + M + + + + + +Atrophy 4 4 4 2 4 2 3 4 4 4 4 4 2 2 3 4 2 2 2 2 2 2
INTEGUMENTARY SYSTEM
Mammary Gland M M M M M M M M M M M M M M M M M M M M M M M M M
Skin + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer 3 3Subcutaneous Tissue, Inflammation, Acute 3Subcutaneous Tissue, Inflammation, Chronic 3
MUSCULOSKELETAL SYSTEM
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 33
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
Bone + + + + + + + + + + + + + + + + + + + + + + + + +Hyperostosis
Skeletal Muscle +
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + +
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic 1ThrombosisAlveolar Epithelium, Hyperplasia 2 1 1Mediastinum, Inflammation, Granulomatous 4
Nose + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation 1 1 1 1 2 1 1Polyp, Inflammatory 1 1Respiratory MetaplasiaOlfactory Epithelium, Accumulation, Hyaline
Droplet1 1
Olfactory Epithelium, Atrophy 1 1 1 3 1 1Olfactory Epithelium, Degeneration 1 2 3Olfactory Epithelium, Respiratory Metaplasia 1 1 1 1 1 2 2 1 1 3 1 2Respiratory Epithelium, Accumulation, Hyaline
Droplet1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1
Respiratory Epithelium, Hyperplasia 1 1 1 1 1 1 1 2 1 2 1 1 1 1 2 1 1 1 1 2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 34
-
DAY ON TEST
B6C3F1 MICE MALE
0610
0686
0637
0731
0717
0731
0731
0731
0731
0133
0730
0732
0596
0730
0732
0732
0731
0655
0716
0732
0732
0732
0437
0732
0731
125 mg/kgANIMAL ID
00101
00102
00103
00104
00105
00106
00107
00108
00109
00110
00111
00112
00113
00114
00115
00116
00117
00118
00119
00120
00121
00122
00123
00124
00125
males(cont...)
Trachea + + + + + + + + + + + + + + + + + + + + + + + + +
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + +Cornea, Inflammation, Chronic
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + +Hyperplasia 2 1 2 3
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + +Metaplasia, OsseousMineralization 1Nephropathy 2 4 1 1 1 1 1 2 2 2 1 2 2 2 2 2 1 1 1 2 1 2Pelvis, Inflammation, Suppurative 2Renal Tubule, CystRenal Tubule, Hyperplasia 1Renal Tubule, Pigmentation 3
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + +
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 35
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + + 50Mineralization 1 2.0
Gallbladder + + + + + + + + + + + + + + + + + + + + + + + + + 49
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + + 50
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + 49Peyer's Patch, Hyperplasia 1 2.0
Liver + + + + A + + + + + + + + + + + + + + + + + + + + 49Basophilic Focus X X 2Clear Cell Focus X X X X X X X X X X X X 22Eosinophilic Focus X X X X X X X X X X X X X X X X X X 35Fatty Change 1 1 1 1 1 2 2 1 2 1 2 2 1 2 1 1 1 2 28 1.5Mixed Cell Focus X X 6Necrosis 2 2 2 2.0
Mesentery 2Fat, Necrosis 2 1.5
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 36
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
Pancreas + + + + + + + + + + + + + + + + + + + + + + + + + 50Acinus, Atrophy 1 1 1.0
Salivary Glands + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 1 1 1 2 1 1 1 1 1 1 1 1 27 1.1Duct, Sublingual Gland, Hyperplasia 1 1.0
Stomach, Forestomach + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Chronic 2 1 2 4 1.8Ulcer 3 3 3 3 7 2.4Epithelium, Hyperplasia 2 2 2 1 4 1.8
Stomach, Glandular + + + + A + + + + + + + + + + + + + + A + + + + + 47Inflammation, Chronic 1 1 1 1 1 1 1 18 1.0Pigmentation 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 38 1.0Epithelium, Hyperplasia 1 1 1 1 1 2 1 1 1 22 1.2
Tooth + + + + + + + + + + + + + + + + + + 32Dysplasia 2 3 2 3 2 3 2 3 3 1 3 3 4 1 1 3 1 3 32 2.5
CARDIOVASCULAR SYSTEM
Blood Vessel + + + + + + + + + + + + + + + + + + + + + + + + 49
Heart + + + + + + + + + + + + + + + + + + + + + + + + + 50Cardiomyopathy 1 1 1 1 1 1 1 1 1 1 1 1 1 1 18 1.0
ENDOCRINE SYSTEM
Adrenal Cortex + + + + + + + + + + + + + + + + + + + + + + + + + 50
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 37
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
Hypertrophy 2 2 2 2.0
Adrenal Medulla + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1.0
Islets, Pancreatic + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 3.0
Parathyroid Gland + + + + + + + + + + + + M + + + + + + + + + + + + 48
Pituitary Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Cyst 1 1 1.0
Thyroid Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50
GENERAL BODY SYSTEM
NONE
GENITAL SYSTEM
Epididymis + + + + + + + + + + + + + + + + + + + + + + + + + 50Infiltration Cellular, Mononuclear Cell 1 3 1.0
Preputial Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Cyst 2 2 2 6 2.0
Prostate + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 1 1 1.0Inflammation, Suppurative 1 2.0
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 38
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
Seminal Vesicle + + + + + + + + + + + + + + + + + + + + + + + + + 50
Testes + + + + + + + + + + + + + + + + + + + + + + + + + 50Germinal Epithelium, Atrophy 2 3 1.3
HEMATOPOIETIC SYSTEM
Bone Marrow + + + + + + + + + + + + + + + + + + + + + + + + + 50
Lymph Node, Mandibular + + + + + + + + + + + + + + + + + + + + + + + + + 50
Lymph Node, Mesenteric + + + + + + + + + + M + + + + + + + + + + + + + + 49
Spleen + + + + + + + + + + + + + + + + + + + + + + + + + 50Accessory Spleen X 1Hematopoietic Cell Proliferation 3 1 1 1 2 2 1 2 1 2 1 2 24 1.8
Thymus + + + + + + M + + + + + + + M + + + + + + + + + + 46Atrophy 2 2 4 2 2 3 2 2 4 4 3 4 4 3 2 4 4 4 2 2 4 4 2 45 3.0
INTEGUMENTARY SYSTEM
Mammary Gland M M M M M M M M M M M M M M M M M M M M M M M M M 0
Skin + + + + + + + + + + + + + + + + + + + + + + + + + 50Ulcer 3 3 3.0Subcutaneous Tissue, Inflammation, Acute 1 3.0Subcutaneous Tissue, Inflammation, Chronic 2 2 2.5
MUSCULOSKELETAL SYSTEM
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 39
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
Bone + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperostosis 1 1 1.0
Skeletal Muscle + 2
NERVOUS SYSTEM
Brain + + + + + + + + + + + + + + + + + + + + + + + + + 50
RESPIRATORY SYSTEM
Lung + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation, Chronic 1 1.0Thrombosis X 1Alveolar Epithelium, Hyperplasia 1 2 5 1.4Mediastinum, Inflammation, Granulomatous 1 4.0
Nose + + + + + + + + + + + + + + + + + + + + + + + + + 50Inflammation 1 1 1 1 1 12 1.1Polyp, Inflammatory 1 3 1.0Respiratory Metaplasia 2 1 2.0Olfactory Epithelium, Accumulation, Hyaline
Droplet1 1 1 5 1.0
Olfactory Epithelium, Atrophy 2 2 1 1 1 11 1.4Olfactory Epithelium, Degeneration 1 4 1.8Olfactory Epithelium, Respiratory Metaplasia 2 2 1 1 2 1 1 2 20 1.5Respiratory Epithelium, Accumulation, Hyaline
Droplet1 2 1 1 1 1 1 1 1 1 1 30 1.1
Respiratory Epithelium, Hyperplasia 2 2 1 2 1 1 1 2 2 1 1 1 1 1 1 1 1 1 1 2 1 41 1.2
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 40
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0731
0598
0731
0637
0617
0732
0732
0580
0655
0702
0730
0731
0695
0731
0732
0731
0697
0732
0567
0732
0731
0731
0732
0732
125 mg/kgANIMAL ID
00126
00127
00128
00129
00130
00131
00132
00133
00134
00135
00136
00137
00138
00139
00140
00141
00142
00143
00144
00145
00146
00147
00148
00149
00150 * TOTALS
Trachea + + + + + + + + + + + + + + + + + + + + + + + + + 50
SPECIAL SENSES SYSTEM
Eye + + + + + + + + + + + + + + + + + + + + + + + + + 50Cornea, Inflammation, Chronic 2 1 2 1.5
Harderian Gland + + + + + + + + + + + + + + + + + + + + + + + + + 50Hyperplasia 2 5 2.0
URINARY SYSTEM
Kidney + + + + + + + + + + + + + + + + + + + + + + + + + 50Metaplasia, Osseous 1 1 1.0Mineralization 1 1.0Nephropathy 1 1 1 2 2 1 1 1 2 1 2 2 1 2 2 1 1 1 1 1 1 2 2 45 1.5Pelvis, Inflammation, Suppurative 1 2.0Renal Tubule, Cyst 1 2 2 1.5Renal Tubule, Hyperplasia 1 1.0Renal Tubule, Pigmentation 1 3.0
Urinary Bladder + + + + + + + + + + + + + + + + + + + + + + + + + 50
Experiment Number: 20006 - 04 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Requested: 06/15/2012Test Type: CHRONIC Indole-3-carbinol Time Report Requested: 12:41:18Route: GAVAGE CAS Number: 700-06-1 First Dose M/F: 04/03/07 / 04/02/07Species/Strain: MICE/B6C3F1 Lab: BAT
* .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade+ .. Tissue examined microscopically M .. Missing tissue 1-4 .. Lesion qualified as:X .. Lesion present A .. Autolysis precludes evaluation 1) Minimal 3) ModerateI .. Insufficient tissue BLANK .. Not examined microscopically 2) Mild 4) Marked
Page 41
-
DAY ON TEST
B6C3F1 MICE MALE
0732
0730
0731
0731
0731
0730
0730
0721
0732
0732
0732
0732
0731
0731
0732
0694
0730
0653
0732
0596
0731
0732
0732
0731
0511
250 mg/kgANIMAL ID
00151
00152
00153
00154
00155
00156
00157
00158
00159
00160
00161
00162
00163
00164
00165
00166
00167
00168
00169
00170
00171
00172
00173
00174
00175
males(cont...)
ALIMENTARY SYSTEM
Esophagus + + + + + + + + + + + + + + + + + + + + + + + + +
Gallbladder + + + + + + + + + + + + + + + + + + M + + + + + +Atrophy 1Inflammation, Suppurative
Intestine Large, Cecum + + + + + + + + + + + + + + + + + + + + + + + + +Ulcer
Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + +Inflammation, Chronic
Intestine Large, Rectum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Duodenum + + + + + + + + + + + + + + + + + + + + + + + + +UlcerEpithelium, Atrophy
Intestine Small, Ileum + + + + + + + + + + + + + + + + + + + + + + + + +
Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + + +Peyer's Patch, Hyperplasia
Liver + + + + + + + + + + + + + + + + + + + + + + + + +Basophilic Focus X XClea