exploiting physical properties in protein structure alignment
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ORAL PRESENTATION Open Access
Exploiting physical properties in protein structurealignmentShweta Shah*, Nick Sahinidis
From Sixth International Society for Computational Biology (ISCB) Student Council SymposiumBoston, MA, USA. 9 July 2010
IntroductionThe functional properties of a protein are stronglydependent on its structural conformation. The primaryquestion addressed in this work is how to determinestructural and therefore functional similarity from 3Dprotein structures. The approach we take relies on pro-tein structure alignment, which elucidates functionalprotein relationships that are not depicted by thesequence.Most current structural alignment tools are based on
geometric properties of protein structures, and do notincorporate the effect of physical properties responsiblefor protein shape and similarity. The effect of physicalfeatures, such as hydrogen bonding, bond and torsionangles, hydropathy, and solvent accessibility, are notwell understood and hence not used while developingstructure alignment tools. In this work, we exploit physi-cal properties in protein structural alignment algorithms.In particular, we incorporate dominant physical featuresin one of the exact protein structure alignment tools,CMOS [1], which is based on the contact map overlapmaximization (MAX-CMO) formulation of proteinstructure alignment. CMOS is a state-of-the-art tool,providing fast structural alignments that are in excellentagreement with the SCOP fold family classification.An implementation of CMOS is accessible at http://eudoxus.cheme.cmu.edu/cmos/cmos.html.
Computational methodology and resultsThe dominant physical features were incorporated inCMOS and their impact on algorithm speed and quality ofresults was studied. Our computational studies indicatethat a search space reduction based on secondary
structures leads to an over five-fold reduction in CPUrequirements while maintaining solution quality [Table 1].Other features, such as hydropathy, torsion angles, andsolvent accessibility, result in alignments with a largedeviation from the optimal alignment.
ConclusionsThe physical features responsible for protein structuralsimilarities are not well understood. While hydropathy,torsion angles, and solvent accessibility were found tobe of little use in this context, our computational studysuggests that hydrogen bonding properties can be mean-ingfully exploited to reduce the search space and reducethe computational requirements of structure alignmentwith CMOS.
Published: 7 December 2010
Reference1. Xie W, Sahinidis NV: A reduction-based exact algorithm for the contact
map overlap problem. Journal of Computational Biology 2007, 14:637-654.
doi:10.1186/1471-2105-11-S10-O3Cite this article as: Shah and Sahinidis: Exploiting physical properties inprotein structure alignment. BMC Bioinformatics 2010 11(Suppl 10):O3.* Correspondence: [email protected]
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh,PA 15213, USA
Table 1 Effect of physical properties on protein structurealignment with CMOS
PhysicalProperty
% matcheseliminated
% deviationfrom optimal
Average speedup in CMOS
SecondaryStructures
24 0.8 5x
Torsionangles
28 14 N.A.
Hydropathy 12 7 N.A.
Solventaccessibility
42 16 N.A.
Shah and Sahinidis BMC Bioinformatics 2010, 11(Suppl 10):O3http://www.biomedcentral.com/1471-2105/11/S10/O3
© 2010 Shah and Sahinidis; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly cited.