Features:

• Automated digital microscopy with up to 100x magnification for whole organism to sub-cellular imaging

• Brightfield, color brightfield, phase contrast and fluorescence with image and laser autofocus to meet a wide range of imaging applications

• Powerful Gen5 3.0 software with seamless image capture, annotation, analysis and movie makers; Requires no extensive training

• Automated kinetic live cell assays, with temperature and CO2/O2

control, humidity and reagent injectors

• Fully integrated and compact design offers quick installation and setup, no extensive training required

capture annotate video analyze

www.biotek.com

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Lionheart™ FX Automated Live Cell Imager는 brightfield, color brightfield, phase contrast(위상차), 형광 채널에서 최대 100x 배율 렌즈 사용과 오일 이멀젼 확대가 가능하여 kinetic live cell imaging에 최적화된 자동 디지털 현미경입니다. 온도, 가스, 습도 조절은 Gen5™ 3.0 소프트웨어에서 완벽하게 조절됩니다. 특별하게 고안된 이 소프트웨어는 샘플 이미지 측정과 분석, 그래픽 주석, 비디오 생성 등을 빠르고 간편하게 이용할 수 있습니다.

All Inclusive Microscopy SystemLionheart FX는 일반적인 현미경의 기능을 뛰어 넘는 자동화된 디지털 이미징 시스템입니다. 기존의 수동 방식의 현미경은 살아있는 세포의 이미지를 측정하기 위하여 복잡하고 다양한 모듈을 이용해야 했습니다. Lionheart FX는 기품 있는 디자인과 더불어 모든 중요한 구성 요소를 이용하기 쉽게 통합하여 작은 사이즈로 제작되었습니다.

Automated Kinetic Live Cell Assay6-에서 1536-well microplates, 세포 배양 접시, 플라스크, 챔버 슬라이드, 카운팅 챔버 등의 다양한 포맷을 지원합니다. 모든 측정 모드에서 1.25x 부터

100x 배율 렌즈를 최대 6개 장작할 수 있고, 오일 이멀젼 전용 렌즈도 있습니다. 최대 40°C 온도 조절 기능과, CO2/O2 조절을 통하여 장시간의 세포 배양과 이미징이 가능합니다. 보다 최적화된 live cell imaging 위하여 항온·항습기와 시약 분주 기능을 추가할 수 있습니다. 장비 내부에 들어오는 빛을 완전히 차단할 수 있도록 제작된 커버를 이용할 수 있습니다.

New Gen5TM 3.0 Software새로운 Gen5 3.0 소프트웨어는 end point, montage, kinetic 이미지 캡처를 빠르고 간편하게 할 수 있도록 업그레이드 되었습니다. 주석달기 기능은 편리하고 실시간 측정한 이미지의 마크업을 하는데 도움이 됩니다. 기초부터 상급까지의 분석 기능도 매우 쉽게 이용할 수 있어 높은 강도의 소프트웨어 교육을 받을 필요가 없습니다.

Wide Range of Imaging Applications이미지 기반 및 레이저 오토포커스(autofocus)를 사용한 Brigthfield, color brightfield, phase contrast, fluorescence의 다양한 이미지 측정 모드로 폭넓은 이미징 어플리케이션을 이용할 수 있습니다.

Specifications:GeneralLabware type: Imaging: 6- to 1536-well platesOther labware: Microscope slides, Petri and cell culture dishes, cell culture flasks

(T25, T75), counting chambers, chamber slides Support for labware up to 1.5” tall

Temperature control: 4-Zone™ incubation to 40 °C with Condensation Control™Variation: ±0.5 °C at 37 °C

CO2 and O 2 control: 0 – 20% CO2 control and 1 – 19% O2 control with optional Gas Controller available

Environment controlcover: Top cover for light tight imaging and incubation control (option)

Stage control: High resolution lead screw driven stage with open accessHumidity control: Humidity chamber with rapid gas recharge (option)Software: Gen5™ 3.0 Data Analysis Software included; Gen5 Image+ and

Image Prime software available for advanced image analysis (option)

ImagingImaging modes: Fluorescence, brightfield, phase contrast, color brightfieldImaging methods: Single color, multi-color, montage, time lapse, Z-stacking, burst modeLight source: High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm,

505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)Camera: 16-bit gray scale, Sony CCD, 1.25 megapixel Camera binning: Optional 2x2 binning for focus and/or image captureCamera exposure

range: 5 milliseconds – 4 secondsImage outputs

available: Raw Images: 16-bit TIFFSaved Images: TIF, JPG, BMP, PNG, EMF, GIFMovies: MP4, WMV

Filter cube capacity: Up to 4 onboard, user-replaceable filter cubesColors available: DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange,

CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em)

Objective capacity: 6 onboard user-replaceable objectivesAvailable objectives: Fluorescence :

Air: 1.25x, 0.04 NA; 2.5x (2.25x eff), 0.07 NA; 2.5x (2.75x eff), 0.12 NA; 4x, 0.13 NA; 10x, 0.30 NA; 20x, 0.45 NA; 40x, 0.60 NA; 60x, 0.70 NAOil: 60x, 1.42 NA; 100x, 1.40 NA

Phase contrast : 4x, 0.13 NA; 10x, 0.30 NA; 20x, 0.45 NA; 40x, 0.60 NA Image collection rate: Single Well Fastest Frame Rate Capture:

*Full Resolution: up to 10 frames per second for single color images*2x2 Binning: up to 20 frames per second for single color images

Automated functions: Autofocus, user-trained autofocus, autoexposure, auto-LED intensity Autofocus method: Image-based autofocus

Laser autofocus option Microscope stage

control: Gen5 Software ControlOptional joystick controller

Reagent Injector Module (optional)Number: 2 syringe pumpsDispense volume: 5 – 1000 µL in 1 µL incrementDispense tip options: Aligned tip – aligned with optical path for dispensing for fast kinetic

assaysOffset tip – dispensing is offset from the optical path

Dead volume: <1.65 mL with back flushLabware supported: 6- to 384-well microplates, Petri and cell culture dishes, chamber slidesDispense precision:Dispense accuracy:

Physical CharacteristicsPower: 250 Watts max.Dimensions: With environment control cover closed or without cover:

18.3” D x 17.9” W x 14.1” H (46.5 cm x 45.5 cm x 35.8 cm)With environment control cover fully open: 18.3” D x 17.9” W x 27.5” H (46.5 cm x 45.5 cm x 69.8 cm)

Weight:With environmental control cover: 58 lbs (26.3 kg)Without environmental control cover: 51 lbs(23.1 kg)

RegulatoryCE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Performance values represent the average observed factory test values.

Optional Accessories:• Environment Control Cover. For temperature control to 40 °C, CO2 /O2 control module support and “darkroom” fluorescence• Humidity Chamber with rapid gas re-charge• Reagent Injector Module• All-in-One HP Desktop Computer• Gen5™ Secure Image Prime for 21 CFR Part 11 compliance• Gen5 Image+ and Image Prime Software for advanced image analysis• Vessel adapters (T25, T75, 35 and 60 mm dishes, slides)

See www.biotek.com for complete list of accessories.

Configurations:LFX: Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield,

color brightfield and phase contrast imaging capabililty. Includes Gen5 3.0 Software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.

*Specifications subject to change. Rev. 3/23/16

Typical Applications:• Live cell imaging • Calcium flux• Cell culture QC • Translocation• Cell proliferation • Cytotoxicity• Apoptosis • Cell viability• 3D cell culture • Wound healing• Tumor invasion • Phagocytosis• Signal transduction • Slide scanning• Stem cell differentiation • H & E imaging• Phenotypic assays • Label-free live cell assays• Cell migration and invasion

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BioTek Instruments Korea Ltd.서울시 강남구 역삼로 7길 9 (역삼동 830-48)T. 02-562-4740 F. 02-562-4750E. korea@biotek.comwww.biotek.com

Environment Control & Injector