fig. s1; ritter et al. 2010 relative induction of p-irak1 relative induction of p-raf relative...
DESCRIPTION
Supplementary Figure 1. Densitometric analysis H. pylori induced phosphorylation of signal transduction protreins and downstream transcription factors. AGS cells were left untreated or were infected with H. pylori strains HP87, HP83, HP42 a HP4 by MOI 10 (white bars) and by MOI 100 (black bars). 30 minutes after infection cells were harvested and whole cellular extracts were isolated. All diagrams illustrate the data from densitometric analysis of detected bands in Western blots ± S.E.M. from three independent infection experiments. The signals were quantified and normalized to corresponding GAPDH signals in each experiment. The level of posphorylated proteins in non nucleofected cells was set to 1 (not shown) and compared to respective signals in infected cells in each experiment to obtain a relative induction factor. Relative induction of phosphorylated protein levels are presented on Y-axis. H. pylori strains are indicated at the bottom of diagram. Data were analyzed for statistical significance by ANOVA test. The symbol * indicates significant difference (P ≤ 0.05) for H. pylori infected AGS cells versus non infected AGS cells.TRANSCRIPT
Fig. S1; Ritter et al. 2010
HP87 HP83 HP42 HP43.6
3.8
4
4.2
4.4
4.6
4.8
5
p-IRAK1
HP87 HP83 HP42 HP40
0.5
1
1.5
2
2.5
3
3.5
p-RAF
HP87 HP83 HP42 HP40
0.51
1.52
2.53
3.54
p-MEK1/2
HP87 HP83 HP42 HP40
0.20.40.60.8
11.21.41.61.8
p-Erk1
HP87 HP83 HP42 HP40
0.5
1
1.5
2
2.5
p-Erk2
Rela
tive
indu
ction
of
p-IR
AK1
Rela
tive
indu
ction
of
p-RA
FRe
lativ
e in
ducti
on o
f p-
MEK
1/2
Rela
tive
indu
ction
of
p-Er
k1Re
lativ
e in
ducti
on o
f p-
Erk2
Rela
tive
indu
ction
of
p-M
KK6/
3
HP87 HP83 HP42 HP40
0.5
1
1.5
2
2.5
p-MKK6/3
Fig. S1; Ritter et al. 2010
HP87 HP83 HP42 HP40
1
2
3
4
5
6
7
8
p-RSK
HP87 HP83 HP42 HP40
1
2
3
4
5
6
7
8
p-JNK
HP87 HP83 HP42 HP40
2
4
6
8
10
12
p-ELK
HP87 HP83 HP42 HP40
0.5
1
1.5
2
2.5
3
3.5
4
4.5
p-Jun
HP87 HP83 HP42 HP43.6
3.8
4
4.2
4.4
4.6
4.8
5
p-ATF2
Rela
tive
indu
ction
of
p-RS
KRe
lativ
e in
ducti
on o
f p-
JNK
Rela
tive
indu
ction
of
p-p6
5
Rela
tive
indu
ction
of
p-EL
KRe
lativ
e in
ducti
on o
f p-
Jun
Rela
tive
indu
ction
of
p-AT
F2
* *
**
HP87 HP83 HP42 HP40
1
2
3
4
5
6
7
p-p65
Supplementary Figure 1. Densitometric analysis H. pylori induced phosphorylation of signal transduction protreins and downstream transcription factors. AGS cells were left untreated or were infected with H. pylori strains HP87, HP83, HP42 a HP4 by MOI 10 (white bars) and by MOI 100 (black bars). 30 minutes after infection cells were harvested and whole cellular extracts were isolated. All diagrams illustrate the data from densitometric analysis of detected bands in Western blots ± S.E.M. from three independent infection experiments. The signals were quantified and normalized to corresponding GAPDH signals in each experiment. The level of posphorylated proteins in non nucleofected cells was set to 1 (not shown) and compared to respective signals in infected cells in each experiment to obtain a relative induction factor. Relative induction of phosphorylated protein levels are presented on Y-axis. H. pylori strains are indicated at the bottom of diagram. Data were analyzed for statistical significance by ANOVA test. The symbol * indicates significant difference (P ≤ 0.05) for H. pylori infected AGS cells versus non infected AGS cells.