Fig. S1. Z-stack of cells in a VZV vesicle smear. (A-H) Slices from bottom to top in increments of 1 mm. Green = autophagosomes; red = VZV gE.

LC3B gE

Fig S2 A. Z-stack of MRC-5 fibroblast cells treated with tunicamycin for 24 h. Slices A1 – A5 from top to bottom in increments of 0.8 mm.

Note LC3B puncta indicative of autophagosomes

A1 A2 A3

A4A5

LC3B DNA

50 mm

Fig. S2 B. Z-stack of MRC-5 fibroblast cels treated with tunicamycin for 24 h. Slices B1 – B5 from top to bottom in increments of 0.5 mm.

Note LC3B puncta indicative of autophagosomes.

B1 B2 B3

B4B5

LC3B DNA

50 mm

Fig S2 C. Z-stack of MRC-5 fibroblast cells treated with tunicamycin for 24 h. Slices C1 – C5 from top to bottom in increments of 0.5 mm.

Note LC3B puncta indicative of autophagosomes.

C1 C2 C3

LC3B DNA

C4C5

50 mm

D4D5Fig S2 D. Z-stack of MRC-5 fibroblast cells

Slices D1 – D5 from bottom to top in increments of 0.5 mm.

D1 D2 D3

LC3B DNA

50 mm

Fig S2 E. Z-stack of MRC-5 fibroblast cells

Slices E1 – E5 from bottom to top in increments of 0.75 mm.

E4E5

E1 E2 E3

LC3B DNA

50 mm

Fig S2 F. Z-stack of MRC-5 fibroblast cells

Slices F1 – F5 from bottom to top in increments of 0.75 mm.

F4F5

F1 F2 F3

LC3B DNA

50 mm

Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h.

FE

A B C

LC3B IE62 DNA

D

50 mm

LK

G H I

J

50 mm

LC3B IE62 DNA

Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h

M N O

50 mmLC3B IE62 DNA

Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h. Green = autophagosomes; red = VZV IE62 protein; blue = Nuclear dye.