fighting climate change at the...
TRANSCRIPT
![Page 1: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/1.jpg)
E. cowli
Fighting Climate Change at the Source
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The Greenhouse Effect Promotes Global Warming
2iGEM Team Braunschweig
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Global Temperature Keeps Rising
iGEM Team Braunschweig 3
Source: NASA
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Why We Target Methane
iGEM Team Braunschweig 4Source: United States Environmental Protection Agency
Methane has a 25 times greater Global Warming Potential than carbon dioxide!
Carbon dioxide
Methane
Others
Greenhouse Gases Global Warming Potential
72%20%
8%
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Cattle – the Unintended Climate Killers
iGEM Team Braunschweig 5Source: NASA‘s Goddard Institute for Space Science
Annual methane emissions
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Spread the Word!
iGEM Team Braunschweig 6
To beef ornot to beef?
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To Beef or not to Beef?
iGEM Team Braunschweig 7
Would you change
your dietary behavior for the sake of the climate?
No
Other
Yes
![Page 8: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/8.jpg)
Previous Approaches Targeted the Microbiota
iGEM Team Braunschweig 8
Hydrogen Methane
Protozoa Methanogens
![Page 9: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/9.jpg)
iGEM Team Braunschweig 9
Hydrogen Methane
Protozoa MethanogensE. cowli
Our Approach Targets Methane
![Page 10: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/10.jpg)
We Fight Global Warming at the Source
iGEM Team Braunschweig 10
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Problems We Have to Face
iGEM Team Braunschweig 11
Ingredient
Production
Activity
Application
Feasibility
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Methylococcus capsulatus Grows too Slowly
doubling times:
E. coli: 20 min
M. capsulatus: 40 h[1]
iGEM Team Braunschweig 12[1] Khalifa 2013
growth curve of Methylococcus capsulatus
x 120
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Reductasesoluble methanemonooxygenase
(sMMO)
Methane Monooxygenase - our Secret Ingredient
CH4 + O2 + NADH + H+ → CH3OH + H2O + NAD+
iGEM Team Braunschweig 13
Hydroxylase
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Reductasesoluble methanemonooxygenase
(sMMO)
Methane Monooxygenase - our Secret Ingredient
CH4 + O2 + NADH + H+ → CH3OH + H2O + NAD+
iGEM Team Braunschweig 14
Hydroxylase
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Boston, We Have a Folding Problem!
iGEM Team Braunschweig 15
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Addition of Chaperones Allows Soluble Expression
iGEM Team Braunschweig 16
Coexpression with chaperones (GroEL, GroES) led to soluble expression of the remaining subunits!
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Addition of Chaperones Allows Soluble Expression
iGEM Team Braunschweig 17
Coexpression with chaperones (GroEL, GroES) led to soluble expression of the remaining subunits!
![Page 18: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/18.jpg)
E. Cowli Was Born!
iGEM Team Braunschweig 18
E. coli E. cowlisoluble methanemonooxygenase
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E. cowli Degrades Methane!
iGEM Team Braunschweig 19
E. coli
E. cowli
The subunits form an active complex – E. cowli works!
2%
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E. cowli Degrades Methane!
iGEM Team Braunschweig 20
E. coli
E. cowli
The subunits form an active complex – E. cowli works!
2%
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Can E. cowli Survive under Rumen Conditions?
iGEM Team Braunschweig 21
Source: Hoards Dairyman
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E. cowli Does not Grow on Rumen Fluid
iGEM Team Braunschweig 22
unfiltratedrumen fluid
E. cowliunfiltratedrumen fluid
E. cowli
+ Glucose – Glucose
inoculated with
agar plateswith 100%
sterile filtratedrumen fluid
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Alginate Beads Can Be Used to Protect E. cowli
iGEM Team Braunschweig 23
„E. cowli is able to grow inside alginatebeads. The ingredients of the beads arein no way toxic for E. cowli or the cow.“
Dr. Ulf Prüße, Thünen Institute Braunschweig
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E. cowli Works in Rumen Fluid
iGEM Team Braunschweig 24
2%
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E. cowli Works in Rumen Fluid
iGEM Team Braunschweig 25
2%
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Cow Farts Are Dangerous!
iGEM Team Braunschweig 26
Source: University of Auckland, New Zealand
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The Model Matches the Measurements
iGEM Team Braunschweig 27
2%
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750 Beads for a Methane-Free Cow
iGEM Team Braunschweig 28
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50 $ per Year for a Better Climate
iGEM Team Braunschweig 29
750 x
110 kg
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50 $ per Year for a Better Climate
iGEM Team Braunschweig 30
50 US$ correspond to 6% of the running costs for keeping a cow!
50 US$
110 kg
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50 $ per Year for a Better Climate
iGEM Team Braunschweig 31
164 milliontonnes
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50 $ per Year for a Better Climate
iGEM Team Braunschweig 32
164 milliontonnes
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50 $ per Year for a Better Climate
iGEM Team Braunschweig 33
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Our Achievements!
iGEM Team Braunschweig 34
Ingredientisolated!
E. cowliengineered!
E. cowliworks!
Applicationapproved!
Feasibilityshown!
![Page 35: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/35.jpg)
13 BioBricks Submitted!
• 10 new BioBricks created
• 2 BioBricks improved
• 1 Protein Generator created:
complete soluble methanemonooxygenase,
easily controllable,
methane-degrading
iGEM Team Braunschweig 35
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Our Thanks Go to …
iGEM Team Braunschweig 36
Prof. Dr. Stefan DübelDr. Torsten Meyer
Tobias Unkauf and Jonas ZantowInstitute of Biochemistry, Biotechnology and Bioinformatics
Technische Universität Braunschweig
Thünen Institute Braunschweigfor technical assistance
iGEM Team Aachenfor providing us with a methane sensor
and all our other supporters!
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37
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Our Intern Benjamin
iGEM Team Braunschweig 38
“These two weeks were eventful and exciting for me … I got a great insight into biotechnology,
especially the lab work.“
![Page 39: Fighting Climate Change at the Source2014.igem.org/files/presentation/Braunschweig_Championship.pdf · The Greenhouse Effect Promotes Global Warming iGEM Team Braunschweig 2. Global](https://reader034.vdocuments.net/reader034/viewer/2022042218/5ec48e3e1ca4b55d3f3cebd5/html5/thumbnails/39.jpg)
Media
iGEM Team Braunschweig 39
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Sources of Methane Emissions
iGEM Team Braunschweig 40
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Safety – Kill Switch
iGEM Team Braunschweig 41
© iGEM Team Peking 2012
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Global Warming Potential
iGEM Team Braunschweig 42http://www.co2crc.com.au/
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Interlab Study
iGEM Team Braunschweig 43
I2020 GFP construct 0K823005 + E0240 GFP construct IK823012 + E0240 GFP construct II
K823005 + K516032 in pSB3K3 RFP construct 0K823005 + K516032 RFP construct IK823012 + K516032 RFP construct II
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MQ-4 Methane Sensor
iGEM Team Braunschweig 44