figure’s1 a c b · g cv vg 13 c g 13 c egfr up egfr dn myc cell cycle b c v g 13 c 0.00 0.05 0.10...
TRANSCRIPT
FIGURE S1A C
D E
B
FIGURE S2
Gefitinib
AZ1366
Combo
V G 13 C
1
V G 13 CV G 13 CV G 13 C V G 13 C V G 13 CEGFR UP EGFR DN MYC
CELL CYCLEB C
V G 13 C0.00
0.05
0.10
FPKM
Axin-2
****
ns
V G 13 C0
2
4
6
8
FPKM
Myc
*
*
**
V G 13 C0
20
40
60
80
100
FPKM
Birc5
* *** *
*
D
V G 13 C0
50
100
150
200
250
FPKM
CCND1
* ** *
**
A
Hierarchial Clustering normalized by geneof z-scored Log2(FPKM+1)
Combo Gefitinib AZ1366 Vehicle
Hierarchial Clustering normalized by geneof z-scored Log2(FPKM+1)
Combo Gefitinib AZ1366 Vehicle
C3 C4 C1 C2 G4 G3 G1 G2 A2 A3 A4 A1 V1 V4 V2 V3
Hierarchial Clustering normalized by geneof z-scored Log2(FPKM+1)
Combo Gefitinib AZ1366 Vehicle
FPKM
FPKM
V G 13 C WNT HALLMARK
p-‐val
Log2(FPKM)AAxin-‐2 c-‐Myc
Survivin Cyclin-‐D1 Veh Gef 1366 Combo0
50
100
*F
G0G1
G2
S
E
FIGURE S3A
B
C
scramble 1-1 1-20.0
0.5
1.0
1.5
*** ***
Axin1 KDscramble 1-1 1-2
0.0
0.5
1.0
1.5
** **
Axin1 KD
scramble 1-1 1-20.0
0.5
1.0
1.5
2.0
** **
Axin1 KD
mRN
A level
(normalized to scram
ble)
0 25 50 75 1000
20000
40000
60000
80000
nM gefitinib
# liv
e ce
lls
gefgef + XAV939
0 25 50 75 1000
20000
40000
60000
80000
100000
nM gefitinib
# liv
e ce
lls
D HCC4006
PC9
**
ns
E
0 1 5 20 500
20000
40000
60000
80000
nM alectinib
# liv
e ce
lls
0103090
H3122 nM AZ1366
Alectinib AZ1366 CI value
1 nM10 nM 1.0130 0.6590 0.47
510 0.75
30 0.8990 0.43
20
10 1.04
30 0.57
90 0.31
5010 0.5630 0.4290 0.23
FIGURE S4A B C
0 25 50 75 1000
20000
40000
60000
80000
nM gefitinib
# liv
e ce
lls
gefgef + az1366
0 25 50 75 1000
20000
40000
60000
80000
nM osimertiinib
# liv
e ce
lls
osiosi + az1366
0 25 50 75 1000
20000
40000
60000
80000
100000
nM gefitinib
# liv
e ce
lls
0 25 50 75 1000
20000
40000
60000
80000
nM osimertinib
# liv
e ce
lls
Gefitinib OsimertinibHCC4006
PC9T790M
* **
nsns
0 50 100 150 200 2500
20
40
60
80
100
Days
Perc
ent s
urvi
val
0 50 100 150 2000
20
40
60
80
100
Days
Perc
ent s
urvi
val
H1650HCC4006
Gef + AZ1366
VehicleGefitinibAZ1366
Vehicle Gef 1366 Gef + 1366-5.0×108
0.0
5.0×108
1.0×109
1.5×109
Lum
ines
cens
e (p
/s)
Vehicle Gef 1366 Gef + 1366-5.0×108
0.0
5.0×108
1.0×109
1.5×109
Lum
ines
cens
e (p
/s)
Vehicle Osi 1366 Osi + 13660
2×108
4×108
6×108
8×108
1×109
Lum
ines
cens
e (p
/s)
HCC4006
H1650
PC9T790M
Vehicle Gef 1366 Gef + 1366-1×1010
0
1×1010
2×1010
3×1010
4×1010
Lum
ines
cens
e (p
/s)
Vehicle Gef 1366 Gef + 1366-5.0×109
0.0
5.0×109
1.0×1010
1.5×1010
Lum
ines
cens
e (p
/s)
Vehicle Osi 1366 Osi + 1366
0
1×1010
2×1010
Lum
ines
cens
e (p
/s)
HCC4006
H1650
PC9T790M
D