final thesis gr 2

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DIET AND LIFESTYLE IN THE COLLEGE POPULATION: ASSOCIATIONS WITH COGNITIVE PERFORMANCE Faoqiya Fatima Christine Filbin Rebecca Fritz Emily Gerlach Phoebe Gulling Emily Walker Mentor: Dr. Bonnie Beezhold

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Page 1: FINAL THESIS GR 2

DIET AND LIFESTYLE IN THE COLLEGE POPULATION:

ASSOCIATIONS WITH COGNITIVE PERFORMANCE

Faoqiya FatimaChristine FilbinRebecca FritzEmily Gerlach

Phoebe GullingEmily Walker

Mentor: Dr. Bonnie Beezhold

Page 2: FINAL THESIS GR 2

Research question

Are diet and lifestyle factors associated with cognitive performance in college students?

Page 3: FINAL THESIS GR 2

Background

• Diet and lifestyle factors play a crucial role in shaping cognitive capacity and brain development1

• Previous research has found a link between diet & lifestyle factors and cognition2,3,4

(1. Gomez-Pinnilla, 2008; 2. Nyaradi, et al., 2014; 3. Yolton, et al., 2004; 4. Zhu, et al., 2014)

Page 4: FINAL THESIS GR 2

Background• Dietary factors (e.g. saturated fat, F&V,

omega-3 fatty acids) and cognitive performance1,2

• Lifestyle factors (e.g. second-hand smoke, physical activity) and cognitive performance3,4

(1. Nyaradi, et al., 2014; 2. Fontani, et al., 2005; 3. Yolton, et al., 2004; 4. Zhu, et. al., 2014)

Page 5: FINAL THESIS GR 2

Study objectives

• Primary objective: To investigate the link between diet and cognitive performance in college students.

• Secondary objective: To investigate the link between lifestyle factors and cognitive performance in college students.

Phoebe Gulling
Maybe have some sort of transition sentence in the script? This is something we could talk about at the next meeting maybe. We need to figure out how to transition from your introduction into the next section. Just something to think about.
Faoqiya Fatima
This slide and script is done, please read it over and make sure you guys are okay with it
Phoebe Gulling
I changed the words "aim" to "objective" to have it better match slide
Page 6: FINAL THESIS GR 2

METHODOLOGY

Page 7: FINAL THESIS GR 2

Study design

• Cross-sectional study• Participants were Benedictine students• Components: online cognitive testing (“brain games”) and online survey completion

• Collaborative planning

Page 8: FINAL THESIS GR 2

Survey – diet questions

• Brain foods (berries, fatty fish, sugar, caffeinated beverages, saturated fat sources)

• General food groups (e.g. F/V, nuts, dairy, sweets, meat)

• Pre-test intake (caffeine, sugar, water, bacon/sausage, cigarette smoking)

• Meal patterns (meals per day, fast-food intake, eating on campus, pre-made foods at home, campus meal plan)

Page 9: FINAL THESIS GR 2

Survey – other questions• Demographic variables (FDU)

• Medications, parental marital status, presence of father • Lifestyle variables (FDU)

• Hours of sleep/night, exercise, relaxation, cigarette and alcohol use, marijuana/recreational drugs, participation in diet to lose weight, recent weight changes, dietary restrictions/allergies, breakfast habits, # meals/day, and supplement use

• Cognitive measures (FDU) • High school/college GPA, ACT/SAT scores, and highest

education level achieved by mother/father

Page 10: FINAL THESIS GR 2

Survey validity & reliability

Validity

• Incorporated validated survey measures • Godin Leisure Time Exercise

Questionnaire• EPIC Norfolk frequencies

• Use of clear and appropriate language for survey questions

• Pilot testing and feedback

Reliability• Potentially unclear questions

were eliminated• Survey measures used strong

consistency within answer choices and question format

• Godin Q; EPIC Norfolk frequencies found to correctly identify low, medium and high consumers of food groups

Page 11: FINAL THESIS GR 2

Cognitive testing

• Cognitive domains most impacted by diet and lifestyle were identified1

• Cogstate provided test battery2

• Validated cognitive testing for clinical trials, healthcare, academic research, brain injury solutions

• Eligible to use Cogstate Research software• Customized battery was selected• Software downloaded on 6 computers in computer lab

(1. DeJager, et al., 2014; 2. Cogstate)

Page 12: FINAL THESIS GR 2

Cognitive testing validity & reliability

Validity

• Instructions were clear and concise

• Cognitive testing was available in all major languages

• Proven ability to detect both improvement and decline in cognitive function1

Reliability

• Minimal learning effects allows testing to be repeated often- even closely together2

• Reliable for all age groups due to sensitivity of testing2

• R values competitive for cognitive testing measures2

• R=(.49-.83)

(1. Cogstate; 2. Nyaradi, et al., 2014)

Page 13: FINAL THESIS GR 2

Processing speed• Ability to take in information, process and extract it1

• Eg: Time it takes to look at a picture and say what it is• Affected by diet/lifestyle in short- and long-term• Groton Maze Timed Chase Test2

• Detection Test2

(1. Turken, et al., 2008; 2. Cogstate)

Page 14: FINAL THESIS GR 2

Verbal learning • Process of acquiring, retaining and recalling of verbal material1• Eg: Hearing a name and remembering it

• Affected by diet/lifestyle in short- and long-term • International Shopping List Test2

(1. Verbal Learning; 2. Cogstate)

Page 15: FINAL THESIS GR 2

Verbal memory

• Ability to recall verbally presented information1

• Eg: Someone tells you a grocery list and you remember it later at the store

• Affected by diet/lifestyle in short- and long-term• International Shopping List Test - Delayed Recall2

(1. Tatsumi, et al., 2008; 2. Cogstate)

Page 16: FINAL THESIS GR 2

Executive function• Set of mental skills that help you get things done; time mgmt, focus, planning, multi-tasking1

• Eg: successfully remembering instructions and carrying out the task

• Affected by diet/lifestyle in short- and long-term• Identification Test2

• Groton Maze Learning Test2

(1. Executive Function & Self-Regulation; 2. Cogstate)

Page 17: FINAL THESIS GR 2

Working memory

• Responsible for: planning, problem solving, multi-tasking, and reasoning; short-term memory1

• Eg: Recalling a recipe while cooking a meal• Affected by diet/lifestyle in short-term• One-Back Memory Test2

(1. McLeod S, 2008; 2. Cogstate)

Page 18: FINAL THESIS GR 2

Visual memory• Ability to correctly identify or reproduce a previously seen image1

• Eg: Looking at the title of a book in class, and then remembering title later at the bookstore

• Affected by diet/lifestyle in short- and long-term• Groton Maze Learning Test - Delayed Recall2

(1. Brady, et al., 2011; 2. Cogstate)

See my comment below, may be too repetetive...-Phoebe Gulling
How specific do we want to get with lifestyle because Phoebe is talking about this.-Faoqiya Fatima
Do we want to find 2 studies for this or use only the Nyaradi article that basically talks about diet and cognition in college students in which a high intake of f/v was associated with better cognition and high intake of sat fats was associated with poorer cognition?-Faoqiya Fatima
I don't think that would be needed... drop out rates don't really have any impact on diet and seem semi-irrelavent...-Phoebe Gulling
Should we go into stats for the reasons for dropping out that i mentioned in my comment in the previous slide?-Faoqiya Fatima
The information we put on this slide and its script will basically be what I will tweak around to create the manuscript-Faoqiya Fatima
For lifestyle factors results slides, I'm already covering a study each on stress and sleep...... So I'm not sure we would want to mention it twice... Did Dr. B say anything about that? Maybe you could introduce studies which cover different lifestyle factors here? I found a good one on smoking, or we could highlight exercise or something?-Phoebe Gulling
Not sure why this statistic is relavent. We aren't talking about college vs. high school graduates.... maybe instead include a stat which talks about college students diet. Like...... studies show that only 14% of college students meet recommendations for fruits/vegetables...-Phoebe Gulling
Income is not relavent here. Also this range is way too large to be meaningful. We need to focus on cognition- as measuring by cognitive domains and diet/lifestyle. is this where we can summarize previous literature on adults/children and cognition/diet and lifestyle-Emily Walker
If drop out/success rates are going to be used as the rational for assessing diet/lifestyle in cognition, thats what the background needs to be. So studies on diet and lifestyle factors in those who have dropped out or succeeded in college. That's what I would think anyways?-Rebecca Fritz
I changed the words "aim" to "objective" to have it better match slide-Phoebe Gulling
This slide and script is done, please read it over and make sure you guys are okay with it-Faoqiya Fatima
Maybe have some sort of transition sentence in the script? This is something we could talk about at the next meeting maybe. We need to figure out how to transition from your introduction into the next section. Just something to think about.-Phoebe Gulling
Page 19: FINAL THESIS GR 2

Recruitment• BU student population

• Flyer distribution, instructor contact/class visits, one- on-one campus recruitment

• Incentives • Eligibility criteria

• 18-24 years old • Current BU student• Not taking prescription

medications for mental conditions

Page 20: FINAL THESIS GR 2

Recruitment• Incentives

• Light snack• Gift cards

• Not eligible for gift card if receiving extra credit

Page 21: FINAL THESIS GR 2

Study protocol• Data collection on six 4-hr am sessions on Thursdays and Fridays in April-May 2016

• Protocol time was ~45 minutes• Informed consent/sign-in; ID number• Cognitive testing• Survey completion • Snack of water, bars, fruit offered; drawing for raffle or

extra credit• Reliability considerations

Page 22: FINAL THESIS GR 2

Statistical analysis

• Correlational, differential • Descriptive statistics• Nonparametric analyses• Multivariate analyses: Chi square tests, Pearson and Spearman correlations

• Univariate analyses: Mann-Whitney U tests, Kruskal Wallis H tests

• Significance - p values < .05; SPSS version 24

Page 23: FINAL THESIS GR 2

RESULTS

Page 24: FINAL THESIS GR 2

Population characteristicsVariable N Mean ± SE

Age 54 21.67 ± 0.20

Gender (m / f) 54 9 / 45

Academic year (lower / upper / grads) 54 8 / 36 / 10

Ethnicity (white / Asian / other) 54 24 / 17 / 13

BMI 54 23.08 ± 0.69

Commuter / Resident 54 46 / 8

Page 25: FINAL THESIS GR 2

Results – Academic year

Freshman

Sophomore

Junior

Senior

Graduate35%

1%

19% 13%

31%

Page 26: FINAL THESIS GR 2

Results – EthnicityHispanic, Latino, or SpanishBlack or African Amer-icanWhite (not Middle Eastern)White (Middle Eastern)Asian

10%8%

47%4%

27%

Page 27: FINAL THESIS GR 2

Variables N Study Mean ± SD

Normative Mean ± SD

Cognitive test scores Identification Test 53 2.73 ± 0.07 2.66 ± 0.08

Groton Maze Timed Chase Test 51 1.49 ± 0.58 -

Groton Maze Learning Test 53 57.45 ± 18.53 40.88 ± 15.34

Groton Maze Learning Test - Delayed Recall 53 9.21 ± 10.01 7.26 ± 5.38

Detection Speed of Performance Test 52 2.55 ± 0.10 2.46 ± 0.09

International Shopping List Test 53 26.21 ± 4.13 25.17 ± 4.30

International Shopping List - Delayed Recall Test

53 9.13 ± 2.13 9.25 ± 3.02

One Back Memory Test 53 1.20 ± 0.28 1.37 ± 0.14

College grade point average (GPA) 53 3.31 ± 0.42 -

Cognitive performance measures

Page 28: FINAL THESIS GR 2

Phoebe Gulling

Research question: What non-diet factors are associated with cognitive performance?

Page 29: FINAL THESIS GR 2

Background – specific demographic / lifestyle factors

• Demographic factors associated with cognitive performance (e.g. father in the home1, socioeconomic status2)

• Lifestyle factors associated with cognitive performance (e.g. stress3, sleep4)

(1. Chapman, 1977; 2. Waber, et al., 2012; 3. Pritchard, 2003; 4. Lo, et al., 2016)

Page 30: FINAL THESIS GR 2

Variable N No YesFather’s education - N/Y college degree 51 25 26Mother’s education - N/Y college degree 53 21 32Parents marital status - N/Y married 54 9 45

Father present in home 54 2 52

Frequency of demographic factors

Page 31: FINAL THESIS GR 2

Frequency of lifestyle factors

Variable N No YesSleep hours/night (<7 / ≥7) 54 39 15

Moderate exercise x/wk (<5x / ≥5X) 54 29 25

Relaxes by reading 54 35 19

Relaxes by religious/spiritual 54 37 17

Relaxes by spending time with pets 54 37 17

Relaxes by listening to music 54 12 42

Relaxes by breathing practices 47 36 11

Cigarette exposure 54 44 10

Alcohol at least once/week 54 42 12

Page 32: FINAL THESIS GR 2

Frequency of meal patternsVariable N No YesDiet to lose weight 54 45 9Weight change in 1 mo 54 37 17Dietary restriction/allergy 54 39 15Eat within 1 hour of waking 54 29 25At least 3 meals/day 54 16 38Commuter>1 meal away from home/wk 46 12 32At least 1 fast food meals/wk 46 16 30ResidentMeal plan 14 9 5Eat at cafeteria 10 5 5At least 1 fast food meal/wk 10 3 7Prepare meals in housing unit 12 3 9

Page 33: FINAL THESIS GR 2

Cognitive performance measures N Father’s

education

Commuter meals

away from home x/wk

Commuter fast food

meals x/wk

Reading for

relaxation N/Y

Pets for relaxation

N/Y

rho / p r / p r / p r / p r / p Detection test (psychomotor function; lower score better)

51 0.29 / 0.040 - - - -

International Shopping List Test-Delayed Recall test (verbal memory; higher score better)

45

- 0.31 / 0.038 - - -

Groton Maze Learning-Delayed Recall test (visual memory; lower score better)

45

53 - - 0.41 / 0.005 - 0.28 /

0.045

International Shopping List Test (verbal learning; higher score better)

53- - - 0.28 /

0.046 -

p < .05 indicates significance; Pearson product-moment and Spearman rank order correlations.

Correlations of lifestyle factor variables with cognitive performance measures

Page 34: FINAL THESIS GR 2

Significant correlations

1. ↑ father’s highest education level : ↓ psychomotor function

2. ↑ commuter total meals away from home : ↑ verbal memory

3. ↑ commuter fast food meals : ↓ visual memory

4. ↑ reading for relaxation : ↑ verbal learning

5. ↑ spending time with pets : ↓ visual memory

Page 35: FINAL THESIS GR 2

Discussion• Consumption of fast food may lower visual memory function • Fast food intake associated with lower test scores among

elementary age students1

• Fast food intake associated with lower GPA among undergraduate students2

• Reading for relaxation may enhance verbal memory• Reading for pleasure associated with higher GPA and greater

desire for higher level thinking among undergraduate students3

(1. Tobin, 2011; 2. Kobayashi, 2009; 3. Turner, et al., 2011)

Page 36: FINAL THESIS GR 2

Emily Walker

Research question: Is meat and poultry intake associated with cognitive performance?

Page 37: FINAL THESIS GR 2

Background• Millennials consume meat at rate similar to previous generations1

• Composition can be both positive and negative for cognition2

• Dietary Guidelines recommend lower intakes of meats, including processed meat

• Studies are conflicting regarding cognition3,4

(1. Midan Marketing, 2016; 2. McAfee, et al., 2010; 3. Baym, et al., 2014; 4. Neumann, et al., 2007)

Page 38: FINAL THESIS GR 2

Methods• Fill in (food/beverage type) times per week: Meat and poultry (beef, buffalo, lamb, pork, chicken, turkey, duck)

• Based on average over past year, check any frequency of (food/beverage type) that you eat/drink: Red meat, processed meat, poultry

• Never or <1x/mo, 1-3x/mo, 1x/wk, 2-4x/wk, 5-6x/wk, 1x/d, 2-3x/d, 4-5x/d, 6+x/d11

(1. EPIC-Norfolk FFQ)

Page 39: FINAL THESIS GR 2

Variable N Freshmen and Sophomores

N = 8JuniorsN = 17

SeniorsN = 19

Graduate Students

N = 10Total meat & poultry/wk

Less than 6 x/wk 32 5 12 9 6

≥6 x/wk 22 3 5 10 4

Red meat/mo Never or <1 x/wk 21 1 11 6 3

> 1 x/wk 31 6 5 13 7

Processed meats/mo

Never or < 1 x/mo 19 0 8 7 4

Monthly, weekly, more 32 7 7 12 6

Poultry/mo Less than 5 x/wk 30 5 11 8 6

≥5 x/wk 21 2 5 11 3

Reporting of meat intake frequency by student academic year

Page 40: FINAL THESIS GR 2

Correlations of meat intake variables with cognitive performance measures

p < .05 indicates significance; Pearson product-moment correlations and Spearman rank order correlations.

Cognitive performance measuresTotal meat/

poultryx/wk

Red meat x/mo

Processed meat x/mo

r / p / n rho / p / n rho / p / nGroton Maze Learning Test (executive function; lower score better)

-0.30 / 0.031n = 53

- -

Groton Maze Learning Test - Delayed Recall (visual memory; lower score better)

-0.44 / 0.001n = 53

-0.36 / 0.010n = 51

-0.35 / 0.012n = 51

International Shopping List - Delayed Recall (verbal memory; higher score better)

0.28 / 0.045

n = 52- -

Page 41: FINAL THESIS GR 2

Cognitive performance measure <6 x/wk ≥6 x/wk

Groton Maze Learning Test - Delayed Recall (visual memory; lower score better)

n = 31Md = 9.00

n = 22Md = 5.00

U = 198.00p = 0.010*; r = -0.36

Groton Maze Learning Test (executive function; lower score better)

n = 31Md = 28.25

n = 22Md = 25.25

U = 302.50p = 0.487; r = -0.10

International Shopping List - Delayed Recall (verbal memory; higher score better)

n = 31Md = 24.00

n = 22Md = 31.23

U = 248.00p = 0.089; r = -0.23

Comparison of cognitive performance measures by total meat/poultry intake frequency weekly

*p < .05 indicates significance; Mann Whitney U tests.

Page 42: FINAL THESIS GR 2

Discussion• Consuming meat was associated with better cognitive performance

• An omnivorous diet may contribute to better cognitive performance1,2

• Nutrients in meat like B12 are related to cognition1,2

(1. Louwman, et al., 2000; 2. Heys, et al., 2010)

Page 43: FINAL THESIS GR 2

Emily Gerlach

Research question: Is egg intake associated with cognitive performance?

Page 44: FINAL THESIS GR 2

Background

•Nutrient dense; may be helpful for cognition

•Studies of key nutrients are conflicting• Saturated fat1

• Choline2,3

(1. Knott, et al., 2015; 2. Poly, et al., 2011; 3. Okereke, et al., 2012)

Page 45: FINAL THESIS GR 2

Methods

•Fill in times per week: Eggs, including yolk

• Whole number response

Page 46: FINAL THESIS GR 2

Variable N

Freshmen and

SophomoresN = 8

JuniorsN = 17

SeniorsN = 19

Graduate Students

N = 10Never or 1x/wk 23 7 8 7 1

2 or more x/wk 30 1 8 12 9

Reporting of weekly egg intake frequency by student academic year

(Statista, 2016)

Page 47: FINAL THESIS GR 2

Correlations of weekly egg intake with cognitive performance measures

p < .05 indicates significance; Pearson product-moment correlation coefficient.

Cognitive performance measure N Egg intake x/wk

r / pCollege GPA 52 0.33 / 0.017

Identification Speed of Performance Test (attention; lower score better)

52 0.29 / 0.037

Page 48: FINAL THESIS GR 2

Comparison of cognitive performance measures in levels of weekly egg intake frequency

Cognitive performance measure

≤1 x/wkN = 22

≥2 x/wkN = 30

College GPA

n = 22Md = 3.20

n = 30Md = 3.50

U = 193.50p = 0.011*; r = 0.35

Identification Speed of Performance Test (attention; lower score better)

n = 22Md = 2.72

n = 30Md = 2.72

U = 310.00p = 0.711; r = 0.05

*p < .05 indicates significance. Mann Whitney U tests.

Page 49: FINAL THESIS GR 2

Discussion• Intake of eggs may be associated with better academic performance

• Breakfast consumption associated with enhanced cognitive function1

• High intakes of saturated fat associated with worse cognition2

(1. Cooper, et al., 2011; 2. Okereke, et al., 2012)

Page 50: FINAL THESIS GR 2

Faoqiya Fatima

Research question: Is dairy intake associated with cognitive performance?

Page 51: FINAL THESIS GR 2

Background

• Composition of dairy may enhance brain function1

• College students have high intake of dairy2

• Previous human studies have linked dairy with enhanced cognition3,4

(1. Camfield, 2011; 2.Durá Travé, 2008; 3. Crichton, et al., 2012; 4. Rahman, et al., 2007)

Page 52: FINAL THESIS GR 2

Methods• Fill in (food/beverage type) times per day: Total dairy foods (milk, cheese, yogurt, etc.)

• Based on average over past year, check any frequency of (food/beverage type) that you eat/drink: • Whole dairy intake (whole milk, cheese, butter)• Yogurt or kefir • Milk (cow’s milk)

• Never or <1x/mo, 1-3x/mo, 1x/wk, 2-4x/wk, 5-6x/wk, 1x/d, 2-3x/d, 4-5x/d, 6+x/d1

Page 53: FINAL THESIS GR 2

Variables NFreshmen and Sophomores

N = 8JuniorsN = 17

SeniorsN = 19

Graduate Students

N = 10Total dairy foods (milk, cheese, yogurt, etc.) Never or 1x/wk 23 7 8 7 1

2 or more x/wk 30 1 8 12 9

Whole dairy intake (whole milk, cheese, butter) Never or less than monthly intake 31 7 9 11 4

Monthly to daily intake 23 1 8 8 6

Yogurt or kefir Never to less than 1x/wk 22 5 5 10 2

1 or more x/wk 31 3 11 9 8

Milk (cow’s milk) Never to 1x/wk 22 3 6 8 5

2 or more x/wk 32 5 11 11 5

Reporting of dairy frequency by student academic year

Page 54: FINAL THESIS GR 2

Correlations of dairy intake variables with cognitive performance measures

Cognitive performance measures Total dairy intake x/day

Whole dairy foods x/mo

r / p / n rho / p / n

Identification Speed of Performance Test (attention; lower score better)

0.34 / 0.014n = 52

-

Detection Speed of Performance Test (psychomotor function; lower score better)

0.48 /0.000n = 51

-0.34 / 0.015n = 51

p < .05 indicates significance; Pearson product-moment and Spearman rank order correlations.

Page 55: FINAL THESIS GR 2

Cognitive performance measures None or 1 x/day 2-7 x/day

Identification Speed of Performance Test (attention; lower score better)

n = 21Md = 2.71

n = 31Md = 2.56

U = 253.0p = 0.176; r = 0.19

Detection Speed of Performance Test (psychomotor function; lower score better)

n = 20Md = 2.74

n = 31Md = 2.53

U = 309.00p = 0.985; r = 0.02

Comparison of cognitive performance variables in levels of total dairy intake frequency

*p < .05 indicates significance; Mann Whitney U tests.

Page 56: FINAL THESIS GR 2

Comparison of cognitive performance variables in levels of whole dairy intake frequency

p values < .05 indicate significance; Mann Whitney U tests.

Cognitive performance measures

Never or less than monthly Monthly to daily

Detection Speed of Performance Test (psychomotor function; lower score better)

n = 31Md = 2.73

n = 21Md = 2.51

U = 191.00p = 0.012*; r = -0.35

*p < .05 indicates significance; Mann Whitney U tests.

Page 57: FINAL THESIS GR 2

Discussion• Dairy intake results were conflicting• Low-fat dairy intake and better cognitive function consensus in studies1,2

• Fat in dairy may increase cognitive function3

(1. Parker, et al., 2012; 2. Crichton, 2012; 3. Bell, n.d.)

Page 58: FINAL THESIS GR 2

Christine Filbin

Research question: Is caffeine intake associated with cognitive performance?

Page 59: FINAL THESIS GR 2

Background• Caffeine acts as stimulant1

• Recommendation <400 mg/day2

• Intake found widely among college-aged students3

• Promotes sustained cognition during fatigue or decreased sleep4

• Decreased reaction time with unchanged accuracy during times of fatigue5

(1. Lakhan, et al., 2012; 2. Mayo Clinic; 3. TCU Nutrition; 4. Kamimori, et al., 2014; 5. van Duinen, et al., 2005)

Page 60: FINAL THESIS GR 2

Methods• Based on average over past year, check any frequency of beverage type

that you drink: Coffee, brewed including coffee drinks• Never or <1x/mo, 1-3x/mo, 1x/wk, 2-4x/wk, 5-6x/wk, 1x/d, 2-3x/d, 4-5x/d, 6+x/d1

• Pre-test Q: Did you drink any of the following caffeinated beverage(s) earlier today?2

12-24 of regular or Diet Coke, Pepsi, or Mountain Dew; black tea (hot or iced) (20-50 mg caffeine)

1 brewed coffee; a small latte/cappuccino; a single espresso shot; a bottled frappuccino; an energy juice, water or tea product; or a small Red Bull or similar brand energy drink (70-120 mg caffeine)

2 or more cups of brewed coffee; a medium or large latte/cappuccino; a double or triple espresso shot; 16 of or more of energy beverages such as Red Bull, Monster Energy, Arizona Green Tea Energy, Jolt Cola, etc.150-250 mg caffeine)

I drank none of these beverage types

(1. EPIC-Norfolk FFQ; 2. Mayo Clinic )

Page 61: FINAL THESIS GR 2

Variable NFreshmen

and Sophomores

N = 8JuniorsN = 17

SeniorsN = 19

Graduate Students

N = 10

Coffee Intake frequency Never or monthly 27 5 9 10 3 Weekly or daily 27 3 8 9 7Pre-Test Caffeine No / Yes 33 / 20 3 / 5 7 / 9 5 / 14 10 / 5

Reporting of caffeine intake by student academic year

Page 62: FINAL THESIS GR 2

p < .05 indicates significance; Spearman rank order correlations.

Cognitive performance measures N Coffee intake

frequencyPre-test caffeine

N/Yrho / p rho / p

Groton Maze Learning Test(executive function; lower score better)

530.28 / 0.042 0.35 / 0.010

Groton Maze Learning Test - Delayed Recall (visual memory; lower score better)

530.41 / 0.002 0.44 / 0.001

Correlations of caffeine intake variables with cognitive performance measures

Page 63: FINAL THESIS GR 2

Cognitive performance measure Never or monthly

Weekly or daily

Groton Maze Learning Test (executive function; lower score better)

n = 27Md = 52.00

n = 27Md = 59.00

U = 266.00p = 0.130; r = 0.28

Groton Maze Learning Test - Delayed Recall (visual memory; lower score better)

n = 27Md = 6.00

n = 27Md = 10.00

U = 189.50p = 0.004*; r = 0.41

Comparison of cognitive performance measures in levels of coffee intake frequency

*p < .05 indicates significance; Mann Whitney U tests.

Page 64: FINAL THESIS GR 2

Cognitive performance measure No Yes

Groton Maze Learning Test (executive function; lower score better)

n = 33Md = 52.00

n = 19Md = 59.00

U = 231.00 p = 0.117; r = 0.35

Groton Maze Learning Test - Delayed Recall (visual memory; lower score better)

n = 33Md = 7.00

n = 19Md = 10.00

U = 196.50p = 0.026*; r = 0.31

Comparison of cognitive performance measures in levels pre-test caffeine intake

*p < .05 indicates significance; Mann Whitney U tests.

Page 65: FINAL THESIS GR 2

Discussion• Intake of caffeine may lower cognitive performance

• Studies show caffeine may worsen cognitive performance1

• Caffeine blocks inhibitory process of adenosine increasing dopamine in the brain2

• Dopamine imbalance may hinder cognitive performance3

(1. Lesk, et al., 2004; 2. Hale, 2012; 3. Nieoullon, et al., 2002)

Page 66: FINAL THESIS GR 2

Becca Fritz

Research question: Is sugar intake associated with cognitive performance?

Page 67: FINAL THESIS GR 2

Background

(1. West, et al., 2006; 2. Cooper, et al., 2015)

• Recommendations: Added sugars not >10% of calories per day

• Elevated blood glucose / insulin associated with poorer cognitive performance1

• The brain may need sustained energy release2

Page 68: FINAL THESIS GR 2

Methods

(1. EPIC-Norfolk FFQ)

• Fill in (food type) times per day: Sweets, not including chocolate (pastries, cakes, pies, cookies, candy, etc.)• Whole number

• Based on average over past year, check any frequency of (beverage type) that you drink: Soft drinks: sugar-sweetened, non-diet • Never or <1x/mo, 1-3x/mo, 1x/wk, 2-4x/wk, 5-6x/wk, 1x/d, 2-

3x/d, 4-5x/d, 6+x/d1

• Pre-test Q: In the past 2 hours have you consumed any sugary foods: (muffins, donuts, cinnamon rolls, etc.)• Check Yes or No

Page 69: FINAL THESIS GR 2

Variables NFreshmen

and Sophomores

N = 8

Juniors N = 17

SeniorsN = 19

Graduate Students

N = 10

Total sweets frequency daily, not including chocolate No intake 22 3 7 6 6 1 or more x/day 31 5 10 12 4Soft drinks; sugar – sweetened, non-diet Never or < 1x/mo 29 2 10 10 7 1 or more x/mo 24 6 6 9 3Pre-test sugary food intake No intake 39 4 9 17 9 Yes intake 15 4 8 2 1

Reporting of sugar intake by student academic year

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p < .05 indicates significance; Pearson product-moment and Spearman rank order correlations.

Cognitive performance measures N

Sugar-sweetened

non diet soft drinks freq

Pre-test sugary

foods NYrho / p r / p

College GPA 52 -0.34 / 0.014 -

Groton Maze Timed Chase Test (visual processing speed; higher score better) 50 -0.35 / 0.014 -

Groton Maze Learning Test (executive function; lower score better) 53 - 0.35 / 0.010

Groton Maze Learning Test – Delayed Recall (visual memory; lower score better) 53 - 0.44 / 0.001

Correlations of sugar intake variables with cognitive performance measures

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Comparison of cognitive performance measures in levels of soft drinks intake frequency

Cognitive performance measures ≤1 x/moN = 29

>1 x/moN = 24

College GPA

n = 28Md = 3.50

n = 24Md = 3.20

U = 179.50p = 0.004*; r = 0.40

Groton Maze Timed Chase Test (visual processing speed; higher score better)

n = 27Md = 1.77

n = 23Md = 1.33

U = 181.00p = 0.012*; r = 0.40

*p < .05 indicates significance; Mann Whitney U tests.

Page 72: FINAL THESIS GR 2

Comparison of cognitive performance measures in levels of pre-test sugary food intake

*p < .05 indicates significance; Mann Whitney U tests.

Cognitive performance measure NoN = 39

YesN = 15

Groton Maze Learning Test (executive function; lower score better)

n = 38Md = 51.0

n = 14Md = 57.5

U = 164.00p = .028*; r = 0.30

Groton Maze Learning Test - Delayed Recall scores (visual memory; lower score better)

n = 39Md = 7

n = 14Md = 10

U = 138.00p = 0.006*; r = 0.37

Page 73: FINAL THESIS GR 2

Discussion• Intake of added sugars may negatively impact cognitive performance

• Poorer memory related to sugar intake1,2

(1. Ye X, et al., 2012; 2. Riby, et al., 2015)

Page 74: FINAL THESIS GR 2

KEY FINDINGS

Page 75: FINAL THESIS GR 2

Conclusion

COGNITIVE

PERFORMANCE

Memory

Verbal learning

Executive function

Attention

College GPA

Processing speed

Psychomotor function

(+) meat & poultry(-) fast food, pre-test caffeine, coffee daily, pre-test sugary foods

(+) reading for relaxation

(+) meat & poultry(-) pre-test caffeine, pre-test sugary foods

(-) eggs weekly, dairy daily

(+) eggs weekly(-) sugar-sweetened soft drinks daily

(-) sugar-sweetened soft drinks daily

(+) whole dairy (-) dairy daily

Page 76: FINAL THESIS GR 2

Strengths• Validated performance

testing measures• Survey questions• Cognitive testing

available in all major languages

• Piloted protocol• Standardized script

during testing• Collaboration with larger

campus

Limitations• Study design• Small sample size• Multiple proctors• Online survey only

available in English• Multiple students in

same room• Potential response bias• Recruitment population• External validity

Page 77: FINAL THESIS GR 2

Future research• Larger population• Comparison of vegan and omnivore cognitive measures

• Experimental study design

Page 78: FINAL THESIS GR 2

Thank you to our sponsors…

Amir Ouranus in IT Dept

Page 79: FINAL THESIS GR 2

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QUESTIONS?