food microbiology (qms) round: 269
TRANSCRIPT
Food Microbiology (QMS)
Round: 269
Issue Number 1
Issued 09 October 2018
LGC | 1 Chamberhall Business Park | Chamberhall Green | Bury | Lancashire | BL9 0AP | UK |+44(0)161 762 2500 | [email protected] | www.lgcstandards.com
Aims of SchemeThe aim of the Quality in Microbiology scheme (QMS) is to enable laboratories performing the microbiological analysis of food and dairy products to monitor their performance and compare it with that of their peers. QMS also aims to provide information to participants on technical issues and methodologies relating to the microbiological examination of food and dairy products.
Further information on the scheme organisation, the test materials, and the statistical analysis of data are available in the QMS Scheme Description and the LGC PT General Protocol.
Performance AssessmentOnce a PT round has closed, the results will be analysed and the assigned value determined for each analyte, according to the criteria provided in the Scheme Description. Information regarding the traceability of each calculated assigned value is also provided in the Scheme Description.
For quantitative data, the participant’s result, x, (or log10 x for microbiological data) is converted into a z score using the following formula;
z = (x - X) SDPA
X = Assigned valueSDPA = Standard deviation for proficiency assessment
For quantitative data, the uncertainty of the assigned value is calculated to ensure that it would have a negligible effect on participants’ performance scores. If the uncertainty of the assigned value is greater than 0.3 x SDPA, then this is not considered negligible. In this situation, a z’ (z prime) performance score is automatically calculated rather than a z score, in order to take account of the measurement uncertainty of the assigned value. The z’ score is calculated using the following formula;
z’ = (x - X) √(SDPA2 + UxAV2)
X = Assigned value SDPA = Standard deviation for proficiency assessmentUxAV= Uncertainty of the assigned value ____ Expanded SDPA = √(SDPA2 + UxAV2)
Trend graphs will use a mixture of z and z’ scores, i.e. the ‘performance score’ for the round.
For quantitative data, gross errors or blunders are removed from the data by removal of any results that are greater than the assigned value ± 5 x SDPA. These results are not used in the final calculation of the assigned value and other summary statistics and will be included in the number of ‘Excluded Results’. All results, including excluded results, will be given a performance score.
For the purposes of performance assessment for a single round, z and z’ scores are interpreted as follows:
z/z’ score Interpretation Colour codingçzç≤ 2.00 Satisfactory result Green2.00 < çzçand < 3.00 Questionable result Amberçzç≥ 3.00 Unsatisfactory result RedNo score given See below No colour coding
Performance scores will not be given for the following:
§ For qualitative results, where satisfactory performance is based on the participants reporting the same result as the assigned result. E.g. detected, not detected. For these results, colour coding of green (satisfactory) or red (unsatisfactory) will apply.
§ For results of zero; such a result is not normally appropriate and should not be reported, the result should be reported as less than the detection limit rather than zero.Note: for a very small number of analytes it may be appropriate to report a result of zero, depending on the type of measurement scale being used.
§ For quantitative results where the analyte under test is present in the test material but participants report zero results or greater than results. In these cases, it is not possible to allocate a performance score and participants should assess their performance based on the assigned value and satisfactory range given.
§ For quantitative results where the analyte under test is present in the test material but participants report a ‘less than’ value. In these cases, it is not possible to allocate a numeric performance score, however, where the ‘less than’ value reported is < (AV-3*SDPA) the ‘less than’ value will be assessed as unsatisfactory (red colour coding), where the less than value reported is between < (AV-3*SDPA) and < (AV-2*SDPA), or > (AV+2*SDPA) the assessment will be questionable (orange colour coding) and it is recommend that you assess whether the method used is fit for purpose, and where the less than value reported is between (AV-2*SDPA and AV+2*SDPA) a satisfactory assessment (green colour coding) will be given as such results are deemed to be consistent with the assigned value.
§ For quantitative results, for microbiological test materials, where the analyte under test is not present in the test material, the assigned value will be classified as ‘Absent’. Results reported as ‘less than’ at or below the detection level for our method of confirmation will be assessed as satisfactory (green colour code). Results reported at a higher detection level will not be assessed and participants will need to use their own judgement to determine whether their result is fit for its intended use. Results reporting a positive count will be assessed as unsatisfactory (red colour code).
§ For quantitative results, for chemistry or clinical test materials, where the analyte under test has not been spiked into the test material, the assigned value will be classified as ‘Zero Spike’. A ‘less than’ value reported at or below the detection level, set as the assigned
Round: 269Scheme: Food Microbiology (QMS)
Scheme Information
Issue: 1 Page 2 of 123 09 October 2018
Round: 269Scheme: Food Microbiology (QMS)the assigned value will be classified as ‘Zero Spike’. A ‘less than’ value reported at or below the detection level, set as the assigned value, will be assessed as satisfactory (green colour code). A ‘less than’ value reported above the detection level will not be assessed and participants will need to use their own judgement to determine whether their result is fit for its intended use. Positive, numeric, results which are below the detection level, set as the assigned value, will not be assessed, whilst those that are greater than the assigned value will be assessed as unsatisfactory (red colour code).
In some cases, performance scores may not be provided or may be provided but with colour coding suspended (indicating that scores need to
be interpreted with caution). For example:
§ For small data sets where less than 8 results have been submitted and the assigned value is derived using a consensus value from the participants’ results. In these circumstances, there may be increased uncertainty of the assigned value, given the low number of participants, and performance scores will be given for information only.
§ In cases where the distribution of the results gives cause for concern e.g. bi-modal data sets. These circumstances will be dependent on the statistical design that is in place.
§ If the assigned value falls below a concentration threshold (only applies to some schemes).
In these or similar circumstances, further explanation as to the reasons for suspension of performance scoring or colour coding, and on the interpretation of results, will be given in the report.
Note: Data displayed in the report will have been rounded to the required number of decimal places. However statistical calculations will have been performed on unrounded data. For this reason, there may appear to be differences between displayed data and calculated data, but this does not affect results in any way.
ConfidentialityA unique laboratory reference code is used to report results in order to ensure confidentiality.
Contact detailsThe scheme coordinator is Karen Jones
Please contact [email protected] if you have any questions or comments regarding the scheme.
Authorisation
This report was authorised by Alexandra Eden, Technical Manager on the 09 October 2018
Issue: 1 Page 3 of 123 09 October 2018
Round: 269Scheme: Food Microbiology (QMS)
Samples were despatched on 03 September 2018The reporting deadline was 28 September 2018
The following samples were distributed in QMS Round 269:
Sample Matrix Contents05D 10g skimmed milk powder Eurotium amstelodami
Zygosaccharomyces bailii
06F 25g oatmeal Salmonella Anatum
07F 25g oatmeal Listeria monocytogenes
08F 10g oatmeal Listeria monocytogenesListeria innocua
09D 10g skimmed milk powder Enterococcus faecalis
10F 10g oatmeal Clostridium perfringens
16D 10g skimmed milk powder Bacillus subtilisEscherichia coli
16F 10g oatmeal Bacillus subtilisEscherichia coli
17D 10g skimmed milk powder Staphylococcus aureusBacillus cereus
17F 10g oatmeal Staphylococcus aureusBacillus cereus
22F 25g oatmeal Escherichia coli O157 (non-toxigenic strain)
23D 10g skimmed milk powder Saccharomyces cerevisiaePenicillium roquefortii
24F 10g oatmeal Saccharomyces cerevisiae
33 Lyophilised vial Enterococcus faecalis
34 Lyophilised vial Salmonella Stanley
35 Paper exercise
40 Lyophilised vial with 375g Salmonella Nottingham Skimmed milk powder
Further information regarding assigned values, performance assessment and technical comments can be found under the individual sample and analyte results.
Sample Details
All homogeneity assessments have been conducted in accordance with the principles stipulated in ISO 13528 [1]. Further details regarding the assessment of homogeneity can be found in the LGC Standards Proficiency Testing General Protocol.
Sample Analyte/Test Method Result05D Osmophilic yeast enumeration DG18 SP 25 Pass
Osmophilic mould enumeration DG18 SP 25 Pass06F Salmonella species detection MKTT/RV + XLD + BGA Pass07F Listeria monocytogenes detection FRASER + ALOA 37 Pass
Listeria species detection FRASER + ALOA 37 Pass08F Total viable count PCA PP 30 Pass09D Total viable count PCA PP 30 Pass10F Clostridium perfringens enumeration TSC PP 37 Pass16D Total viable count PCA PP 30 Pass16F Total viable count PCA PP 30 Pass17D Total viable count PCA PP 30 Pass17F Total viable count PCA PP 30 Pass22F Escherichia coli O157 detection BROTH + CT-SMAC Pass23D Yeast enumeration RB SP 25 Pass
Mould enumeration RB SP 25 Pass24F Yeast enumeration RB SP 25 Pass33 Purity/Identification NA SP 30 Pass34 Purity/Identification NA SP 30 Pass
Quality Control
Issue: 1 Page 4 of 123 09 October 2018
Round: 269Scheme: Food Microbiology (QMS)34 Purity/Identification NA SP 30 Pass40 Salmonella species detection MKTT/RV + XLD + BGA Pass
For quantitative testing, one-way ANOVA is used to estimate the analytical and sampling variance. For the sample to be considered sufficiently homogeneous for use in the PT scheme, the calculated sampling variance must be less than a critical value calculated according to the procedure described in the ISO 13528 [1].
For qualitative testing, the target analyte must be detected in 100% of test materials analysed.
For any analyte which has not been proven to be sufficiently homogeneous, and any closely related analytes, the value set for the SDPA may be suspended in order to take account of any potential inhomogeneity. The actual value used for the standard deviation for proficiency assessment is shown at the foot of the results and z-score tables in this report.
Often a particular test material does not require homogeneity assessment prior to distribution. Such sample types include standard solutions and aqueous solutions.
Test materials provided under the LGC scope of accreditation are assessed to ensure that they are sufficiently stable for the duration of the PT round. If test materials are subsequently shown to have changed significantly through the time of the PT round either, the effect of the instability will be quantified and taken into account in the performance evaluation or participant performance will not be evaluated.
[1] ISO 13528 (2015), ‘Statistical methods for use in proficiency testing by inter-laboratory comparison’.
Issue: 1 Page 5 of 123 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic yeast
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.2915,166DG18 PP 25MC0136
-0.418,600DG18 SP 25MC0159
-0.846,100OtherMC0261
0.8624,000DG18 SP 25MC0396
-0.537,800DG18 SP 25MC0738
0.2915,182DG18 SP 25MC0752
-0.0211,800DG18 PP 25MC0999
0.2815,000DG18 SP 25MC1026
0.1013,000DG18 PP 25MC1065
-0.378,900DG18 SP 25MC1149
0.6921,000DG18 PP 25MC1184
-0.1410,700DG18 PP 25MC1480
2.3580,000DG18 SP 25MC1509
0.0012,000DG18 PP 25MC1509
0.0012,000DG18 SP 25MC1510
-0.2310,000DG18 SP 25MC1512
-0.478,200DG18 SP 25MC1601
-0.1111,000DG18 PP 25MC1601
0.2815,000DG18 PP 25MC1602
-0.557,700DG18 SP 25MC1602
0.1013,000OtherMC1602
-0.1111,000OtherMC1610
0.2815,000OtherMC1611
-0.369,000OtherMC1619
0.2815,000DG18 PP 25MC1663
-0.0511,500OtherMC1701
0.0712,727DG18 SP 25MC1712
0.6320,000DG18 SP 25MC1939
0.8423,600OtherMC2043
0.6219,800DG18 SP 25MC2163
-0.1111,000DG18 PP 25MC2220
-0.299,500DG18 PP 25MC2294
-0.339,200DG18 SP 25MC2315
-0.378,900DG18 SP 25MC2517
-1.095,000DG18 PP 25MC2577
0.7522,000OtherMC2619
0.5618,852OtherMC2627
0.6320,000DG18 SP 25MC2824
0.6320,000OtherMC2824
0.5719,000DG18 PP 25MC2951
0.1013,000DG18 PP 25MC2978
-1.244,400DG18 SP 25MC2988
-0.677,000DG18 SP 25MC3000
-0.0411,600DG18 SP 25MC3002
-0.0111,900DG18 SP 25MC3003
0.0012,000DG18 SP 25MC3113
0.2314,500DG18 SP 25MC3163
-0.866,000RB PP 25MC3163
0.1513,500OtherMC3163
0.3616,000DG18 SP 25MC3228
-1.533,500DG18 SP 25MC3463
-0.587,500DG18 PP 25MC3471
1.0929,000OtherMC3591
0.1914,000DG18 SP 25MC3699
0.1013,000DG18 PP 25MC4015
-1.563,400OtherMC4061
-2.811,250DG18 SP 25MC4061
-0.0411,600DG18 PP 25MC4077
0.5018,000OtherMC4093
-0.637,200OtherMC4356
-0.2310,000DG18 SP 25MC4439
Page 6 of 123Issue: 1 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic yeast
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
1.0929,000DG18 SP 25MC4666
-0.2310,000DG18 PP 25MC4680
-0.1111,000DG18 SP 25MC4680
-0.378,900DG18 SP 25MC4690
-1.463,700DG18 SP 25MC4736
-0.1111,000DG18 SP 25MC4828
-1.104,950DG18 SP 25MC4836
0.0412,400DG18 SP 25MC4852
-1.364,000DG18 SP 25MC5291
-1.723,000DG18 SP 25MC5566
0.1013,000DG18 SP 25MC5792
-0.587,500DG18 SP 25MC5866
-0.607,400DG18 SP 25MC5939
0.1713,800DG18 SP 25MC5940
-1.244,400DG18 SP 25MC5979
0.3616,000OtherMC6090
0.1914,000DG18 PP 25MC6120
0.3616,000OtherMC6120
0.1914,000DG18 PP 25MC6270
0.0012,000DG18 SP 25MC6270
0.2815,000OtherMC6270
0.1013,000OtherMC6344
0.4317,000DG18 PP 25MC6353
0.6320,000OtherMC6353
<10OtherMC6434
0.7522,000DG18 SP 25MC6437
0.1914,000DG18 SP 25MC6440
0.3616,000OtherMC6496
1.0127,000DG18 SP 25MC6508
0.0012,000OtherMC6508
-2.162,100OtherMC6717
-2.002,400OtherMC6723
1.0227,270DG18 SP 25MC6933
-0.309,400DG18 SP 25MC6955
0.2815,000RB PP 25MC7133
0.5018,000DG18 SP 25MC7730
-0.537,800DG18 SP 25MC7732
0.2815,000DG18 PP 25MC7745
0.6921,000OtherMC7745
0.5819,090DG18 SP 25MC7862
0.2514,700DG18 SP 25MC8154
-0.657,100OtherMC8196
-0.1111,000OtherMC9560
Value
Data Statistics
104Number of Results
1Number of Excluded Results
4.05 log10Mean
4.08 log10Median
0.26 log10Standard Deviation
0.19 log10Robust Standard Deviation
1250 to 80000 cfu/gResult Range
Page 7 of 123Issue: 1 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic yeast
Round: 269Scheme: Food Microbiology (QMS)
Performance Statistics
Value
12,000 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
2,394 to 60,142 cfu/gSatisfactory Range
97.1%Satisfactory z scores
2.9%Questionable z scores
0.0%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
96.21250 to80000
0.224.0750052DG18 SP 25
100.05000 to21000
0.104.1121.15022DG18 PP 25
100.06000 to15000
0.303.981.9202RB PP 25
96.32100 to29000
0.224.1326.92128Other
97.11250 to80000
0.194.081001104All
Comments
The sample contained Zygosaccharomyces bailii at an assigned value of 12,000 cfu/g.
Osmophiles are organisms that are restricted to high solute environments. This is usually a high sugar environment where water activity islow.
Page 8 of 123Issue: 1 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.547,600DG18 SP 25MC0136
0.376,600DG18 SP 25MC0159
0.537,500OtherMC0261
1.1112,000DG18 SP 25MC0396
-0.254,000DG18 SP 25MC0738
0.426,864DG18 SP 25MC0752
0.256,000DG18 PP 25MC0999
-2.25800DG18 SP 25MC1026
0.547,600DG18 SP 25MC1149
0.759,000DG18 PP 25MC1184
-4.71110DG18 PP 25MC1480
0.004,900DG18 PP 25MC1509
-0.034,800DG18 SP 25MC1509
0.276,100DG18 SP 25MC1510
0.035,000DG18 SP 25MC1512
1.1112,000DG18 SP 25MC1601
-0.114,500DG18 PP 25MC1601
0.256,000DG18 PP 25MC1602
0.195,700DG18 SP 25MC1602
-0.613,000OtherMC1602
0.256,000OtherMC1610
<10OtherMC1611
-1.851,100OtherMC1619
-0.254,000DG18 PP 25MC1663
1.0911,818DG18 SP 25MC1712
0.759,000DG18 SP 25MC1939
<10OtherMC2043
<20DG18 SP 25MC2163
-0.054,700DG18 PP 25MC2220
0.497,300DG18 PP 25MC2294
-0.034,800DG18 SP 25MC2315
0.416,800DG18 SP 25MC2517
-0.453,400DG18 PP 25MC2577
-0.164,300OtherMC2619
-1.391,600OtherMC2627
1.9724,000DG18 SP 25MC2824
-0.224,100DG18 PP 25MC2951
0.396,700DG18 PP 25MC2978
0.256,000DG18 SP 25MC2988
-0.114,500DG18 SP 25MC3000
0.075,200DG18 SP 25MC3002
-1.311,700DG18 SP 25MC3003
-0.134,400DG18 SP 25MC3113
0.035,000DG18 SP 25MC3163
0.587,833RB PP 25MC3163
0.356,500OtherMC3163
0.587,800DG18 SP 25MC3228
0.125,400DG18 SP 25MC3463
-0.134,400DG18 PP 25MC3471
-0.792,600DG18 SP 25MC3699
-0.652,900DG18 PP 25MC4015
-1.112,000DG18 PP 25MC4061
-1.082,050OtherMC4061
0.447,000DG18 PP 25MC4077
-0.254,000OtherMC4093
<10OtherMC4356
-3.11400DG18 SP 25MC4439
<100DG18 SP 25MC4666
-2.25800DG18 PP 25MC4680
-0.493,300DG18 SP 25MC4680
<100DG18 SP 25MC4690
Page 9 of 123Issue: 1 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.792,600DG18 SP 25MC4736
0.256,000DG18 SP 25MC4828
0.014,950DG18 SP 25MC4836
-3.11400DG18 SP 25MC4852
-0.383,600DG18 SP 25MC5291
-0.842,500DG18 SP 25MC5566
1.0011,000DG18 SP 25MC5792
-0.613,000DG18 SP 25MC5937
-2.02960DG18 SP 25MC5939
-0.383,600DG18 SP 25MC5940
-0.453,400DG18 SP 25MC5979
1.0011,000OtherMC6090
<10DG18 PP 25MC6270
<10DG18 SP 25MC6270
<10OtherMC6270
-0.164,300OtherMC6344
-1.971,000DG18 PP 25MC6353
-2.10900OtherMC6353
0.256,000OtherMC6434
0.829,500DG18 SP 25MC6437
0.618,000DG18 SP 25MC6440
0.718,700OtherMC6496
0.517,400DG18 SP 25MC6508
-0.493,300OtherMC6508
0.004,900OtherMC6717
<10DG18 SP 25MC6933
-0.692,800DG18 SP 25MC6955
0.145,500RB PP 25MC7133
-3.47300DG18 SP 25MC7730
0.416,800DG18 SP 25MC7732
0.105,300DG18 PP 25MC7745
0.447,000OtherMC7745
-0.732,727DG18 SP 25MC7862
0.487,200DG18 SP 25MC8154
<10OtherMC8196
<10OtherMC9560
Value
Data Statistics
97Number of Results
12Number of Excluded Results
3.60 log10Mean
3.69 log10Median
0.39 log10Standard Deviation
0.25 log10Robust Standard Deviation
110 to 24000 cfu/gResult Range
Performance Statistics
Value
4,900 cfu/gAssigned Value
0.03 log10Uncertainty of Assigned Value
0.35 log10SDPA
978 to 24,558 cfu/gSatisfactory Range
90.6%Satisfactory z scores
4.7%Questionable z scores
4.7%Unsatisfactory z scores
Page 10 of 123Issue: 1 09 October 2018
Sample: 05D - Osmophilic Yeast & Mould
Analyte: Osmophilic mould
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
89.4300 to 240000.273.7053.61552DG18 SP 25
89.5110 to 90000.193.6520.62120DG18 PP 25
100.05500 to 78330.113.822.0602RB PP 25
94.1900 to 110000.273.6323.71623Other
90.6110 to 240000.253.691001297All
Comments
The sample contained Eurotium amstelodami at an assigned value of 4,900 cfu/g.
Osmophiles are organisms that are restricted to high solute environments. This is usually a high sugar environment where water activity islow.
Page 11 of 123Issue: 1 09 October 2018
Analyte: Salmonella species
Sample: 06F - Salmonella species (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedPCRMC0055
DetectedOtherMC0143
DetectedMKTT/RV + XLD + BGA 37MC0143
DetectedPCRMC0143
DetectedELISAMC0159
DetectedMKTT/RV + XLD + BGA 37MC0212
DetectedPCRMC0217
DetectedMKTT/RV + XLD + BGA 37MC0302
Not DetectedOtherMC0309
DetectedOtherMC0379
DetectedBroth 37/agar 37 (Various)MC0431
DetectedOtherMC0434
DetectedMKTT/RV + XLD + BGA 37MC0437
DetectedPCRMC0437
DetectedMKTT/RV + XLD + BGA 37MC0440
DetectedOtherMC0440
DetectedMKTT/RV + XLD + BGA 37MC0483
DetectedOtherMC0483
DetectedPCRMC0483
DetectedPCRMC0495
DetectedOtherMC0579
Not DetectedChromogenic agarMC0689
DetectedMKTT/RV + XLD + BGA 37MC0738
DetectedChromogenic agarMC0746
DetectedChromogenic agarMC0854
DetectedMKTT/RV + XLD + BGA 37MC0854
DetectedOtherMC0988
DetectedVIDASMC0988
DetectedOtherMC1065
DetectedPCRMC1065
DetectedVIDASMC1065
DetectedMKTT/RV + XLD + BGA 37MC1073
DetectedMKTT/RV + XLD + BGA 37MC1096
DetectedMKTT/RV + XLD + BGA 37MC1097
DetectedVIDASMC1097
DetectedOtherMC1159
DetectedOtherMC1165
DetectedChromogenic agarMC1166
DetectedMKTT/RV + XLD + BGA 37MC1226
DetectedMKTT/RV + XLD + BGA 37MC1301
DetectedPCRMC1301
DetectedPCRMC1365
DetectedRapid test (Various)MC1449
DetectedChromogenic agarMC1449
DetectedOtherMC1450
DetectedVIDASMC1483
DetectedMKTT/RV + XLD + BGA 37MC1493
DetectedChromogenic agarMC1509
DetectedVIDASMC1509
DetectedChromogenic agarMC1601
DetectedPCRMC1601
DetectedChromogenic agarMC1602
DetectedOtherMC1602
DetectedELISAMC1638
DetectedChromogenic agarMC1640
DetectedOtherMC1653
DetectedRapid test (Various)MC1653
DetectedMKTT/RV + XLD + BGA 37MC1721
DetectedPCRMC1721
DetectedMKTT/RV + XLD + BGA 37MC1727
DetectedOtherMC1764
DetectedOtherMC1876
Issue: 1 Page 12 of 123 09 October 2018
Analyte: Salmonella species
Sample: 06F - Salmonella species (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedMKTT/RV + XLD + BGA 37MC1895
DetectedOtherMC1895
DetectedVIDASMC1920
DetectedOtherMC1946
DetectedOtherMC2108
DetectedOtherMC2149
DetectedMKTT/RV + XLD + BGA 37MC2163
DetectedVIDASMC2163
DetectedELISAMC2163
DetectedMKTT/RV + XLD + BGA 37MC2214
DetectedOtherMC2220
DetectedMKTT/RV + XLD + BGA 37MC2240
DetectedPCRMC2282
DetectedChromogenic agarMC2338
DetectedPCRMC2357
DetectedVIDASMC2360
DetectedChromogenic agarMC2492
DetectedMKTT/RV + XLD + BGA 37MC2492
DetectedMKTT/RV + XLD + BGA 37MC2569
DetectedPCRMC2569
DetectedChromogenic agarMC2588
DetectedMKTT/RV + XLD + BGA 37MC2798
DetectedVIDASMC2813
DetectedPCRMC2813
DetectedMKTT/RV + XLD + BGA 37MC2850
DetectedMKTT/RV + XLD + BGA 37MC2851
DetectedOtherMC2851
DetectedMKTT/RV + XLD + BGA 37MC2951
DetectedRapid test (Various)MC2989
DetectedVIDASMC3000
DetectedPCRMC3000
DetectedMKTT/RV + XLD + BGA 37MC3002
DetectedELISAMC3003
DetectedChromogenic agarMC3102
DetectedMKTT/RV + XLD + BGA 37MC3102
DetectedOtherMC3102
DetectedBroth 37/agar 37 (Various)MC3180
DetectedMKTT/RV + XLD + BGA 37MC3228
DetectedOtherMC3228
DetectedVIDASMC3228
DetectedOtherMC3242
DetectedPCRMC3243
DetectedPCRMC3244
DetectedPCRMC3245
DetectedPCRMC3246
DetectedPCRMC3248
DetectedVIDASMC3249
DetectedPCRMC3250
DetectedPCRMC3251
DetectedPCRMC3252
DetectedOtherMC3253
DetectedPCRMC3254
DetectedMKTT/RV + XLD + BGA 37MC3255
DetectedVIDASMC3255
DetectedBroth 37/agar 37 (Various)MC3256
DetectedVIDASMC3256
DetectedMKTT/RV + XLD + BGA 37MC3392
DetectedMKTT/RV + XLD + BGA 37MC3399
DetectedPCRMC3415
DetectedPCRMC3471
DetectedMKTT/RV + XLD + BGA 37MC3474
DetectedMKTT/RV + XLD + BGA 37MC3477
Issue: 1 Page 13 of 123 09 October 2018
Analyte: Salmonella species
Sample: 06F - Salmonella species (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedRapid test (Various)MC3477
DetectedMKTT/RV + XLD + BGA 37MC3493
DetectedELISAMC3519
DetectedChromogenic agarMC3611
DetectedPCRMC3694
DetectedMKTT/RV + XLD + BGA 37MC3704
DetectedVIDASMC3744
DetectedOtherMC3744
DetectedPCRMC3745
DetectedOtherMC3746
DetectedPCRMC3746
DetectedVIDASMC3746
DetectedChromogenic agarMC3771
DetectedVIDASMC3824
DetectedBroth 37/agar 37 (Various)MC3838
DetectedMKTT/RV + XLD + BGA 37MC3984
DetectedChromogenic agarMC3990
DetectedBioControl AssuranceMC4158
DetectedMKTT/RV + XLD + BGA 37MC4174
DetectedOtherMC4174
DetectedVIDASMC4201
DetectedVIDASMC4262
DetectedBroth 37/agar 37 (Various)MC4364
DetectedPCRMC4369
DetectedVIDASMC4371
DetectedVIDASMC4372
DetectedVIDASMC4385
DetectedPCRMC4424
Not DetectedChromogenic agarMC4495
DetectedPCRMC4688
DetectedChromogenic agarMC4688
DetectedMKTT/RV + XLD + BGA 37MC4695
DetectedMKTT/RV + XLD + BGA 37MC4710
DetectedOtherMC4710
DetectedDuPont BAXMC4722
DetectedOtherMC4722
DetectedMKTT/RV + XLD + BGA 37MC4739
DetectedVIDASMC4752
DetectedMKTT/RV + XLD + BGA 37MC4797
DetectedRapid test (Various)MC4851
DetectedVIDASMC4851
DetectedOtherMC4870
DetectedMKTT/RV + XLD + BGA 37MC4870
DetectedOtherMC4918
DetectedMKTT/RV + XLD + BGA 37MC4957
DetectedBroth 37/agar 37 (Various)MC5009
DetectedDuPont BAXMC5026
DetectedMKTT/RV + XLD + BGA 37MC5027
Not DetectedMKTT/RV + XLD + BGA 37MC5094
Detected3M Molecular Detection SystemMC5127
DetectedMKTT/RV + XLD + BGA 37MC5127
DetectedOtherMC5127
Not DetectedVIDASMC5510
DetectedOtherMC5527
DetectedMKTT/RV + XLD + BGA 37MC5676
DetectedMKTT/RV + XLD + BGA 37MC5683
DetectedMKTT/RV + XLD + BGA 37MC5696
DetectedBroth 37/agar 37 (Various)MC5741
DetectedELISAMC5937
DetectedELISAMC5939
DetectedELISAMC5940
DetectedOtherMC5969
Issue: 1 Page 14 of 123 09 October 2018
Analyte: Salmonella species
Sample: 06F - Salmonella species (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedPCRMC5969
DetectedVIDASMC5970
DetectedPCRMC5989
DetectedDuPont BAXMC6008
DetectedVIDASMC6008
DetectedOtherMC6008
DetectedMKTT/RV + XLD + BGA 37MC6163
DetectedMKTT/RV + XLD + BGA 37MC6258
DetectedMKTT/RV + XLD + BGA 37MC6268
DetectedMKTT/RV + XLD + BGA 37MC6295
DetectedVIDASMC6366
DetectedMKTT/RV + XLD + BGA 37MC6380
DetectedChromogenic agarMC6400
DetectedMKTT/RV + XLD + BGA 37MC6421
DetectedMKTT/RV + XLD + BGA 37MC6446
DetectedOtherMC6446
DetectedVIDASMC6446
DetectedOtherMC6447
DetectedVIDASMC6447
DetectedMKTT/RV + XLD + BGA 37MC6456
DetectedMKTT/RV + XLD + BGA 37MC6476
DetectedVIDASMC6524
Detected3M Molecular Detection SystemMC6558
DetectedMKTT/RV + XLD + BGA 37MC6564
Not DetectedRapid test (Various)MC6645
Detected3M Molecular Detection SystemMC6669
DetectedVIDASMC6679
DetectedMKTT/RV + XLD + BGA 37MC6745
DetectedELISAMC6767
DetectedMKTT/RV + XLD + BGA 37MC6778
Detected3M Molecular Detection SystemMC6778
DetectedPCRMC6798
DetectedOtherMC6801
DetectedMKTT/RV + XLD + BGA 37MC6903
DetectedVIDASMC6948
DetectedChromogenic agarMC6970
DetectedPCRMC6970
DetectedMKTT/RV + XLD + BGA 37MC7057
DetectedTECRAMC7057
DetectedBroth 37/agar 37 (Various)MC7057
DetectedMKTT/RV + XLD + BGA 37MC7099
DetectedChromogenic agarMC7112
DetectedVIDASMC7189
DetectedDuPont BAXMC7259
DetectedVIDASMC7259
DetectedMKTT/RV + XLD + BGA 37MC7288
DetectedOtherMC7288
DetectedMKTT/RV + XLD + BGA 37MC7289
DetectedOtherMC7289
DetectedDuPont BAXMC7291
DetectedOtherMC7291
DetectedMKTT/RV + XLD + BGA 37MC7299
DetectedOtherMC7371
DetectedPCRMC7419
Detected3M Molecular Detection SystemMC7438
DetectedOtherMC7438
DetectedVIDASMC7490
DetectedOtherMC7583
DetectedMKTT/RV + XLD + BGA 37MC7729
DetectedOtherMC7729
DetectedVIDASMC7729
DetectedOtherMC7756
Issue: 1 Page 15 of 123 09 October 2018
Analyte: Salmonella species
Sample: 06F - Salmonella species (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedOtherMC7757
DetectedMKTT/RV + XLD + BGA 37MC7796
DetectedOtherMC7799
DetectedOtherMC7800
DetectedOtherMC7801
DetectedOtherMC7869
DetectedMKTT/RV + XLD + BGA 37MC7937
DetectedOtherMC7955
Detected3M Molecular Detection SystemMC7992
Detected3M Molecular Detection SystemMC7998
DetectedPCRMC7998
DetectedDuPont BAXMC8055
DetectedMKTT/RV + XLD + BGA 37MC8152
DetectedPCRMC8196
DetectedMKTT/RV + XLD + BGA 37MC8255
DetectedMKTT/RV + XLD + BGA 37MC8312
DetectedOtherMC8343
DetectedChromogenic agarMC8387
DetectedMKTT/RV + XLD + BGA 37MC8387
DetectedChromogenic agarMC8949
DetectedOtherMC8949
DetectedOtherMC9143
DetectedPCRMC9291
DetectedOtherMC9335
DetectedMKTT/RV + XLD + BGA 37MC9335
DetectedPCRMC9526
DetectedOtherMC9568
Result
Data Statistics
DetectedAssigned Value
275Number of Results
98%Satisfactory
Methodology Summary
% SatisfactoryMethod
99%MKTT/RV + XLD + BGA 37
100%Broth 37/agar 37 (Various)
97%VIDAS
100%TECRA
83%Rapid test (Various)
91%Chromogenic agar
100%PCR
100%ELISA
100%3M Molecular Detection System
100%BioControl Assurance
100%DuPont BAX
98%Other
Comments
The sample contained Salmonella Anatum at an approximate inoculum level of 3 cfu/g. The assigned value is therefore Salmonella species ‘detected’.
Salmonella Anatum is characteristic of the genus on selective media and in confirmatory tests, being H2S positive, motile, lactose negative,citrate positive and urease negative. It is a group E1 Salmonella according to the Kauffmann-White Scheme, with a full serological profile of‘O’ antigen: 3, 10; ‘H’ phase 1 antigen: e, h and ‘H’ phase 2 antigen: e, h.
Salmonellae are Gram-negative non-spore forming rods that are oxidase negative and catalase positive, and are members of theEnterobacteriaceae family. Almost all Salmonella serotypes cause acute gastro-enteritis and account for a large proportion of all foodpoisoning outbreaks. Primary sources of Salmonellae are the gastrointestinal tract of animals; the organism can be transmitted via faecesinto soil, water, feeds, foods and other animals, including humans.
Issue: 1 Page 16 of 123 09 October 2018
Analyte: Listeria species
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedFraser + ALOA 37MC0143
DetectedOtherMC0143
DetectedPCRMC0143
DetectedFraser + ALOA 37MC0212
DetectedOtherMC0379
DetectedOtherMC0431
DetectedFraser + ALOA 37MC0434
DetectedFraser + ALOA 37MC0437
DetectedOtherMC0440
DetectedRapid L.monoMC0440
DetectedVIDASMC0440
DetectedFraser + ALOA 37MC0456
DetectedOtherMC0456
DetectedFraser + ALOA 37MC0483
DetectedOtherMC0483
DetectedPCRMC0483
DetectedRapid L.monoMC0495
DetectedOtherMC0579
DetectedPCRMC0676
DetectedFraser + ALOA 37MC0854
DetectedFraser + ALOA 37MC0914
DetectedVIDASMC1065
DetectedFraser + PL 37MC1073
DetectedFraser + ALOA 37MC1096
DetectedFraser + ALOA 37MC1097
DetectedFraser + ALOA 37MC1139
DetectedFraser + ALOA 37MC1160
DetectedFraser + ALOA 37MC1226
DetectedFraser + ALOA 37MC1438
DetectedFraser + ALOA 37MC1449
DetectedBroth 37/agar 37 (Various)MC1449
DetectedOtherMC1450
DetectedFraser + ALOA 37MC1493
DetectedFraser + ALOA 37MC1509
DetectedOtherMC1509
DetectedVIDASMC1509
DetectedPCRMC1601
DetectedRapid L.monoMC1601
DetectedFraser + ALOA 37MC1601
DetectedVIDASMC1602
DetectedOtherMC1653
DetectedVIDASMC1653
DetectedFraser + ALOA 37MC1721
DetectedFraser + ALOA 37MC1727
DetectedRapid L.monoMC1764
DetectedFraser + ALOA 37MC1796
DetectedOtherMC1876
DetectedOtherMC1895
DetectedFraser + ALOA 37MC1895
DetectedFraser + ALOA 37MC1920
Not DetectedFraser + ALOA 37MC1925
DetectedVIDASMC1926
DetectedOtherMC1946
DetectedVIDASMC2072
DetectedFraser + ALOA 37MC2214
DetectedVIDASMC2220
DetectedFraser + ALOA 37MC2240
DetectedOtherMC2357
DetectedOtherMC2468
DetectedFraser + ALOA 37MC2492
DetectedOtherMC2492
DetectedFraser + ALOA 37MC2798
Issue: 1 Page 17 of 123 09 October 2018
Analyte: Listeria species
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedVIDASMC2813
DetectedFraser + ALOA 37MC2823
DetectedRapid L.monoMC2850
DetectedBroth 37/agar 37 (Various)MC2851
DetectedOtherMC2851
DetectedFraser + ALOA 37MC2902
DetectedFraser + ALOA 37MC2905
DetectedOtherMC2989
DetectedVIDASMC3000
DetectedFraser + ALOA 37MC3109
DetectedOtherMC3123
DetectedFraser + ALOA 37MC3228
DetectedOtherMC3242
DetectedPCRMC3243
DetectedOtherMC3244
DetectedPCRMC3245
DetectedPCRMC3246
DetectedOtherMC3248
DetectedPCRMC3249
DetectedPCRMC3250
DetectedPCRMC3251
DetectedPCRMC3252
DetectedOtherMC3253
DetectedOtherMC3254
DetectedVIDASMC3255
DetectedVIDASMC3256
DetectedFraser + ALOA 37MC3392
DetectedFraser + ALOA 37MC3415
DetectedBroth 37/agar 37 (Various)MC3423
DetectedFraser + ALOA 37MC3423
DetectedOtherMC3423
DetectedOtherMC3462
DetectedFraser + ALOA 37MC3583
DetectedFraser + ALOA 37MC3588
DetectedVIDASMC3744
DetectedPCRMC3745
DetectedOtherMC3746
DetectedVIDASMC3746
DetectedVIDASMC3939
DetectedFraser + ALOA 37MC3984
DetectedFraser + ALOA 37MC4029
DetectedOtherMC4093
DetectedVIDASMC4201
DetectedVIDASMC4262
DetectedFraser + ALOA 37MC4339
DetectedFraser + ALOA 37MC4364
DetectedVIDASMC4371
DetectedVIDASMC4372
DetectedVIDASMC4385
DetectedFraser + PL 37MC4680
DetectedFraser + PL 37MC4688
DetectedBroth 37/agar 37 (Various)MC4710
DetectedOtherMC4710
DetectedFraser + ALOA 37MC4720
Detected3M Molecular Detection SystemMC4722
DetectedVIDASMC4752
DetectedFraser + ALOA 37MC4851
DetectedVIDASMC4851
DetectedFraser + ALOA 37MC4870
DetectedOtherMC4870
DetectedBroth 37/agar 37 (Various)MC5026
DetectedFraser + PL 37MC5027
Issue: 1 Page 18 of 123 09 October 2018
Analyte: Listeria species
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
Not DetectedVIDASMC5094
DetectedELISAMC5174
DetectedVIDASMC5510
DetectedFraser + ALOA 37MC5517
DetectedFraser + ALOA 37MC5528
Not DetectedELISAMC5531
Not DetectedFraser + ALOA 37MC5531
DetectedFraser + ALOA 37MC5676
DetectedFraser + ALOA 37MC5881
DetectedOtherMC5881
DetectedVIDASMC5989
DetectedFraser + ALOA 37MC6008
DetectedVIDASMC6008
DetectedFraser + ALOA 37MC6092
DetectedOtherMC6123
DetectedFraser + ALOA 37MC6163
DetectedFraser + ALOA 37MC6182
DetectedFraser + ALOA 37MC6215
DetectedFraser + ALOA 37MC6268
DetectedFraser + ALOA 37MC6295
DetectedRapid L.monoMC6336
DetectedPCRMC6353
DetectedFraser + PL 37MC6353
DetectedFraser + ALOA 37MC6421
DetectedFraser + ALOA 37MC6446
DetectedRapid L.monoMC6446
DetectedOtherMC6447
DetectedFraser + ALOA 37MC6476
DetectedOtherMC6524
DetectedFraser + ALOA 37MC6564
Detected3M Molecular Detection SystemMC6669
DetectedFraser + ALOA 37MC6798
DetectedFraser + ALOA 37MC6833
DetectedFraser + ALOA 37MC6903
DetectedOtherMC6948
DetectedOtherMC7057
DetectedBroth 37/agar 37 (Various)MC7057
DetectedFraser + PL 37MC7057
DetectedFraser + ALOA 37MC7099
DetectedFraser + ALOA 37MC7112
DetectedVIDASMC7189
DetectedFraser + ALOA 37MC7259
DetectedVIDASMC7259
DetectedOtherMC7288
DetectedFraser + ALOA 37MC7289
DetectedFraser + PL 37MC7289
DetectedDuPont BAXMC7291
DetectedOtherMC7291
DetectedFraser + ALOA 37MC7352
DetectedPCRMC7419
DetectedVIDASMC7490
DetectedVIDASMC7598
DetectedFraser + ALOA 37MC7729
DetectedVIDASMC7729
DetectedFraser + ALOA 37MC7937
DetectedFraser + ALOA 37MC7946
DetectedFraser + ALOA 37MC7955
Detected3M Molecular Detection SystemMC7992
DetectedFraser + ALOA 37MC8312
DetectedFraser + ALOA 37MC8949
DetectedFraser + PL 37MC8949
DetectedVIDASMC8949
Issue: 1 Page 19 of 123 09 October 2018
Analyte: Listeria species
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedOtherMC9143
DetectedVIDASMC9511
Result
Data Statistics
DetectedAssigned Value
188Number of Results
98%Satisfactory
Methodology Summary
% SatisfactoryMethod
97%Fraser + ALOA 37
100%Fraser + PL 37
100%Broth 37/agar 37 (Various)
100%Rapid L.mono
100%PCR
97%VIDAS
50%ELISA
100%3M Molecular Detection System
100%DuPont BAX
100%Other
Comments
The sample contained Listeria monocytogenes at an approximate inoculum level of 4 cfu/g. The assigned value is therefore Listeria species ‘detected’.
There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.
Issue: 1 Page 20 of 123 09 October 2018
Analyte: Listeria monocytogenes
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedFraser + ALOA 37MC0143
DetectedOtherMC0143
DetectedPCRMC0143
DetectedFraser + ALOA 37MC0212
DetectedOtherMC0431
DetectedFraser + ALOA 37MC0434
DetectedFraser + ALOA 37MC0437
DetectedPCRMC0437
DetectedOtherMC0440
DetectedRapid L.monoMC0440
DetectedVIDASMC0440
DetectedFraser + ALOA 37MC0456
DetectedOtherMC0456
DetectedFraser + ALOA 37MC0483
DetectedPCRMC0483
DetectedRapid L.monoMC0495
DetectedPCRMC0676
DetectedFraser + ALOA 37MC0854
DetectedOtherMC0854
DetectedFraser + ALOA 37MC1019
DetectedOtherMC1065
DetectedFraser + PL 37MC1073
DetectedFraser + ALOA 37MC1096
DetectedFraser + ALOA 37MC1097
DetectedVIDASMC1097
DetectedFraser + ALOA 37MC1139
DetectedOtherMC1159
DetectedFraser + ALOA 37MC1160
DetectedFraser + ALOA 37MC1226
DetectedFraser + ALOA 37MC1438
DetectedFraser + ALOA 37MC1449
DetectedRapid L.monoMC1449
DetectedOtherMC1450
DetectedVIDASMC1483
DetectedFraser + ALOA 37MC1493
DetectedFraser + PL 37MC1509
DetectedFraser + ALOA 37MC1588
DetectedVIDASMC1588
DetectedPCRMC1601
DetectedRapid L.monoMC1601
DetectedFraser + ALOA 37MC1601
DetectedFraser + ALOA 37MC1602
DetectedRapid L.monoMC1602
DetectedOtherMC1653
DetectedVIDASMC1653
DetectedFraser + ALOA 37MC1684
DetectedFraser + ALOA 37MC1721
DetectedPCRMC1721
DetectedFraser + ALOA 37MC1727
DetectedRapid L.monoMC1764
DetectedFraser + ALOA 37MC1796
DetectedFraser + ALOA 37MC1876
DetectedOtherMC1895
DetectedFraser + ALOA 37MC1895
DetectedVIDASMC1920
DetectedFraser + ALOA 37MC1925
DetectedVIDASMC1926
DetectedOtherMC1946
DetectedVIDASMC2072
DetectedOtherMC2108
DetectedPCRMC2109
DetectedFraser + ALOA 37MC2169
Issue: 1 Page 21 of 123 09 October 2018
Analyte: Listeria monocytogenes
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedFraser + ALOA 37MC2214
DetectedVIDASMC2220
DetectedFraser + ALOA 37MC2240
DetectedPCRMC2282
DetectedFraser + ALOA 37MC2468
DetectedFraser + ALOA 37MC2492
DetectedOtherMC2492
DetectedBroth 37/agar 37 (Various)MC2582
DetectedOtherMC2588
Not DetectedFraser + ALOA 37MC2798
DetectedVIDASMC2813
DetectedFraser + ALOA 37MC2823
DetectedRapid L.monoMC2850
DetectedBroth 37/agar 37 (Various)MC2851
DetectedOtherMC2851
DetectedFraser + ALOA 37MC2902
DetectedFraser + ALOA 37MC2905
DetectedOtherMC2989
DetectedVIDASMC3000
DetectedBroth 37/agar 37 (Various)MC3102
DetectedFraser + ALOA 37MC3102
DetectedRapid L.monoMC3102
DetectedFraser + ALOA 37MC3109
DetectedOtherMC3123
DetectedFraser + ALOA 37MC3228
DetectedVIDASMC3228
DetectedPCRMC3242
DetectedPCRMC3243
DetectedOtherMC3244
DetectedPCRMC3245
Not DetectedPCRMC3246
DetectedOtherMC3248
DetectedPCRMC3249
DetectedOtherMC3250
DetectedPCRMC3251
DetectedPCRMC3252
DetectedOtherMC3253
DetectedOtherMC3254
DetectedVIDASMC3255
DetectedVIDASMC3256
DetectedFraser + ALOA 37MC3392
DetectedBroth 37/agar 37 (Various)MC3413
DetectedPCRMC3415
DetectedBroth 37/agar 37 (Various)MC3423
DetectedFraser + ALOA 37MC3423
DetectedOtherMC3423
DetectedFraser + ALOA 37MC3493
DetectedFraser + ALOA 37MC3583
DetectedFraser + ALOA 37MC3588
DetectedVIDASMC3744
DetectedPCRMC3746
DetectedVIDASMC3782
DetectedFraser + ALOA 37MC3984
DetectedFraser + ALOA 37MC3990
DetectedFraser + ALOA 37MC4029
DetectedOtherMC4093
DetectedFraser + ALOA 37MC4174
DetectedOtherMC4174
DetectedVIDASMC4201
DetectedVIDASMC4262
DetectedFraser + ALOA 37MC4339
DetectedFraser + ALOA 37MC4364
Issue: 1 Page 22 of 123 09 October 2018
Analyte: Listeria monocytogenes
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedPCRMC4369
DetectedPCRMC4372
DetectedVIDASMC4385
DetectedPCRMC4424
DetectedFraser + PL 37MC4680
DetectedOtherMC4680
DetectedPCRMC4688
DetectedFraser + ALOA 37MC4688
DetectedVIDASMC4688
DetectedBroth 37/agar 37 (Various)MC4710
DetectedOtherMC4710
DetectedFraser + ALOA 37MC4720
DetectedVIDASMC4752
DetectedFraser + ALOA 37MC4851
DetectedFraser + ALOA 37MC4870
DetectedOtherMC4870
DetectedOtherMC5009
DetectedBroth 37/agar 37 (Various)MC5026
DetectedFraser + PL 37MC5027
Not DetectedFraser + ALOA 37MC5094
DetectedELISAMC5174
DetectedVIDASMC5510
DetectedFraser + ALOA 37MC5517
Detected3M Molecular Detection SystemMC5528
DetectedELISAMC5531
DetectedFraser + ALOA 37MC5531
DetectedFraser + ALOA 37MC5676
DetectedOtherMC5774
DetectedPCRMC5821
DetectedFraser + ALOA 37MC5881
DetectedOtherMC5881
DetectedOtherMC5969
DetectedFraser + ALOA 37MC5989
DetectedFraser + ALOA 37MC6008
DetectedDuPont BAXMC6008
DetectedVIDASMC6008
DetectedFraser + ALOA 37MC6092
DetectedOtherMC6123
DetectedFraser + ALOA 37MC6163
DetectedFraser + ALOA 37MC6182
DetectedFraser + ALOA 37MC6215
DetectedFraser + ALOA 37MC6258
DetectedFraser + ALOA 37MC6268
DetectedFraser + ALOA 37MC6295
DetectedRapid L.monoMC6336
DetectedPCRMC6353
DetectedVIDASMC6353
DetectedFraser + ALOA 37MC6353
DetectedVIDASMC6366
DetectedFraser + ALOA 37MC6421
DetectedFraser + ALOA 37MC6446
DetectedRapid L.monoMC6446
DetectedOtherMC6447
DetectedRapid L.monoMC6447
DetectedFraser + ALOA 37MC6476
DetectedOtherMC6524
DetectedFraser + ALOA 37MC6564
Detected3M Molecular Detection SystemMC6669
DetectedVIDASMC6679
Not DetectedFraser + ALOA 37MC6767
DetectedFraser + ALOA 37MC6798
DetectedFraser + ALOA 37MC6833
Issue: 1 Page 23 of 123 09 October 2018
The sample contained Listeria monocytogenes at an approximate inoculum level of 4 cfu/g. The assigned value is therefore Listeria
Analyte: Listeria monocytogenes
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedFraser + ALOA 37MC6903
DetectedOtherMC6948
DetectedFraser + ALOA 37MC7057
DetectedBroth 37/agar 37 (Various)MC7057
DetectedFraser + PL 37MC7057
DetectedFraser + ALOA 37MC7099
DetectedFraser + ALOA 37MC7112
DetectedVIDASMC7189
DetectedFraser + ALOA 37MC7259
DetectedVIDASMC7259
DetectedOtherMC7288
DetectedFraser + ALOA 37MC7289
DetectedFraser + PL 37MC7289
DetectedDuPont BAXMC7291
DetectedOtherMC7291
DetectedFraser + ALOA 37MC7352
DetectedPCRMC7419
Detected3M Molecular Detection SystemMC7438
DetectedOtherMC7438
DetectedVIDASMC7490
DetectedFraser + ALOA 37MC7729
DetectedVIDASMC7729
Not DetectedPCRMC7760
DetectedOtherMC7852
DetectedFraser + ALOA 37MC7937
DetectedFraser + ALOA 37MC7946
DetectedFraser + ALOA 37MC7955
Detected3M Molecular Detection SystemMC7992
Detected3M Molecular Detection SystemMC7998
DetectedPCRMC7998
DetectedBroth 37/agar 37 (Various)MC8152
DetectedPCRMC8185
DetectedFraser + ALOA 37MC8312
DetectedFraser + ALOA 37MC8949
DetectedFraser + PL 37MC8949
DetectedVIDASMC8949
DetectedOtherMC9143
DetectedPCRMC9291
DetectedVIDASMC9511
DetectedPCRMC9526
Result
Data Statistics
DetectedAssigned Value
226Number of Results
98%Satisfactory
Methodology Summary
% SatisfactoryMethod
97%Fraser + ALOA 37
100%Fraser + PL 37
100%Broth 37/agar 37 (Various)
100%Rapid L.mono
93%PCR
100%VIDAS
100%ELISA
100%3M Molecular Detection System
100%DuPont BAX
100%Other
Comments
Issue: 1 Page 24 of 123 09 October 2018
Analyte: Listeria monocytogenes
Sample: 07F - Listeria (P/A)
Round: 269Scheme: Food Microbiology (QMS)
The sample contained Listeria monocytogenes at an approximate inoculum level of 4 cfu/g. The assigned value is therefore Listeria monocytogenes ‘detected’.
There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.
Issue: 1 Page 25 of 123 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.7251,000OtherMC0199
-1.1935,000OtherMC0495
-0.2773,000ALOA SP 37MC0689
0.44130,000ALOA SP 37MC0699
0.0494,000ALOA SP 37MC1065
0.19106,000ALOA SP 37MC1073
-0.0488,000OtherMC1149
-0.1680,000ALOA SP 37MC1159
-0.3668,000ALOA SP 37MC1160
-0.2972,000OtherMC1165
0.98200,000ALOA SP 37MC1297
0.44130,000ALOA SP 37MC1393
0.0393,000OtherMC1509
-0.2475,000OtherMC1555
0.62150,000OtherMC1601
0.44130,000ALOA SP 37MC1601
0.34120,000PL SP 37MC1601
-0.2177,000OtherMC1602
-3.376,000ALOA SP 37MC1603
-0.4663,000OtherMC1603
-0.0687,000OtherMC1619
0.86181,250PL SP 37MC1680
0.62150,000OtherMC1680
0.24110,000ALOA SP 37MC1695
0.12100,000OtherMC1764
-0.6056,000ALOA SP 37MC1937
0.27113,000ALOA SP 37MC2210
0.34120,000OtherMC2220
0.0393,000OtherMC2410
0.34120,000ALOA SP 37MC2468
0.34120,000OtherMC2479
-0.1680,000ALOA SP 37MC2696
-0.5260,000ALOA SP 37MC2823
0.62150,000ALOA SP 37MC2868
0.34120,000ALOA SP 37MC2902
0.66155,500ALOA SP 37MC2932
-0.0488,000ALOA SP 37MC2988
-0.1382,000OtherMC2988
-0.7251,000ALOA SP 37MC3000
0.54141,000PL SP 37MC3111
-0.0389,000ALOA SP 37MC3113
0.68158,000OtherMC3123
0.34120,000ALOA SP 37MC3228
0.70160,000OtherMC3228
-1.3830,000OtherMC3242
0.44130,000ALOA SP 37MC3423
0.34120,000OtherMC3423
-0.2972,000OtherMC3477
-0.2972,000PL SP 37MC3477
-4.043,500ALOA SP 37MC3588
0.29115,000OtherMC3591
-0.9941,000ALOA SP 37MC3611
-0.0190,000ALOA SP 37MC3645
-0.5857,000ALOA SP 37MC3794
0.1099,000OtherMC4356
0.32117,574OtherMC4424
-0.0488,000ALOA SP 37MC4483
0.24110,000ALOA SP 37MC4666
0.44130,000ALOA SP 37MC4690
-0.4464,000ALOA SP 37MC4709
-0.2276,000ALOA SP 37MC4870
Page 26 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.1099,000ALOA SP 37MC4901
-0.0885,000ALOA SP 37MC4918
-0.1680,000ALOA SP 37MC5517
-0.2177,000ALOA SP 37MC5528
>30,000ALOA SP 37MC5531
-0.6056,000ALOA SP 37MC5566
-0.8745,000OtherMC5566
-0.9941,000PL SP 37MC5566
0.24110,000ALOA SP 37MC5755
0.31117,000ALOA SP 37MC6060
0.0897,000ALOA SP 37MC6215
-0.5658,000ALOA SP 37MC6353
-0.8745,200OtherMC6366
-0.5956,666ALOA SP 37MC6514
0.54141,000ALOA SP 37MC6526
0.25111,000ALOA SP 37MC6564
-0.0190,000ALOA SP 37MC6645
0.44130,000PL SP 37MC6722
-0.5260,000OtherMC6750
0.0192,000ALOA SP 37MC6809
-0.0190,000OtherMC6809
0.21108,000ALOA SP 37MC6948
0.0393,000ALOA SP 37MC7255
0.19106,000ALOA SP 37MC7352
0.85180,000ALOA SP 37MC7560
0.20107,000ALOA SP 37MC7583
-2.819,455ALOA SP 37MC8170
-2.0118,000OtherMC8940
0.34120,000ALOA SP 37MC8949
-0.0786,000OtherMC9125
-0.0190,000ALOA SP 37MC9282
-2.3014,200OtherMC9549
Value
Data Statistics
93Number of Results
1Number of Excluded Results
4.91 log10Mean
4.96 log10Median
0.29 log10Standard Deviation
0.18 log10Robust Standard Deviation
3500 to 200000 cfu/gResult Range
Performance Statistics
Value
90,995 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
18,156 to 456,055 cfu/gSatisfactory Range
94.6%Satisfactory z scores
3.3%Questionable z scores
2.2%Unsatisfactory z scores
Page 27 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria species
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
94.53500 to200000
0.164.9760.22156ALOA SP 37
100.041000 to181250
0.165.106.4506PL SP 37
93.514200 to160000
0.214.9433.33031Other
94.63500 to200000
0.184.96100193All
Comments
The sample contained Listeria monocytogenes and Listeria innocua at an assigned value of 90,995 cfu/g.
There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.
Page 28 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria monocytogenes
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.41100,000ALOA SP 37MC0141
-0.4351,000OtherMC0199
-0.9035,000OtherMC0495
0.0273,000ALOA SP 37MC0689
0.41100,000ALOA SP 37MC0699
-0.2758,000ALOA SP 37MC0915
0.0072,000OtherMC0915
-0.3156,000ALOA SP 37MC1065
0.2588,000PL SP 37MC1073
-0.3056,364OtherMC1079
-0.9733,000OtherMC1149
-0.5347,000ALOA SP 37MC1159
-0.8337,000ALOA SP 37MC1160
-0.3156,000ALOA SP 37MC1165
0.41100,000ALOA SP 37MC1297
0.2890,000ALOA SP 37MC1387
0.2890,000ALOA SP 37MC1393
0.0776,000ALOA SP 37MC1463
0.73130,000OtherMC1467
0.53110,000ALOA SP 37MC1509
-0.4550,000OtherMC1509
-0.0569,000OtherMC1538
-0.7041,000OtherMC1555
0.4099,000OtherMC1601
0.53110,000ALOA SP 37MC1601
-0.0569,000PL SP 37MC1601
-0.3654,000ALOA SP 37MC1602
-0.6144,000OtherMC1602
0.61118,000ALOA SP 37MC1603
0.0072,000OtherMC1603
0.2387,000OtherMC1619
-0.0370,000ALOA SP 37MC1630
0.41100,000ALOA SP 37MC1680
0.83140,000OtherMC1680
0.0575,000ALOA SP 37MC1695
0.41100,000OtherMC1764
-0.3654,000ALOA SP 37MC1895
-0.4351,000OtherMC1895
-0.2758,000ALOA SP 37MC2031
0.0072,000ALOA SP 37MC2210
0.3293,000OtherMC2220
0.2185,000OtherMC2330
0.0273,000ALOA SP 37MC2388
-2.777,700PL SP 37MC2409
0.3293,000OtherMC2410
-0.5845,000ALOA SP 37MC2468
0.1078,000OtherMC2479
-0.2161,000ALOA SP 37MC2577
-0.3156,000ALOA SP 37MC2696
0.0575,000ALOA SP 37MC2742
0.53110,000ALOA SP 37MC2868
0.0877,000ALOA SP 37MC2882
0.2185,000ALOA SP 37MC2902
0.42100,900ALOA SP 37MC2932
0.0776,000ALOA SP 37MC2988
-0.1365,000OtherMC2988
-1.0930,000OtherMC3000
0.2285,909OtherMC3011
-0.2559,000ALOA SP 37MC3102
-0.1365,000OtherMC3102
-0.5347,000ALOA SP 37MC3113
Page 29 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria monocytogenes
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.4099,000OtherMC3123
0.1380,000ALOA SP 37MC3228
0.2588,000OtherMC3228
-0.2559,000ALOA SP 37MC3284
-0.2857,580OtherMC3376
0.0575,000ALOA SP 37MC3399
-0.0768,000OtherMC3399
0.63120,000ALOA SP 37MC3423
0.41100,000OtherMC3423
0.0072,000OtherMC3477
0.0072,000PL SP 37MC3477
-0.2758,000ALOA SP 37MC3478
<100ALOA SP 37MC3588
0.0575,000OtherMC3591
-0.9035,000ALOA SP 37MC3611
-0.2360,000ALOA SP 37MC3638
0.0575,000ALOA SP 37MC3645
0.1883,000OtherMC3782
-0.6742,000ALOA SP 37MC3794
-0.3156,000ALOA SP 37MC4029
-0.5347,000OtherMC4356
-1.0132,000OtherMC4372
-0.1364,696OtherMC4424
-0.5347,000ALOA SP 37MC4483
0.53110,000ALOA SP 37MC4666
0.1682,000ALOA SP 37MC4690
-1.0531,000ALOA SP 37MC4709
0.2185,000ALOA SP 37MC4851
-0.1564,000OtherMC4870
-0.1365,000ALOA SP 37MC4870
0.2588,000ALOA SP 37MC4885
0.0072,000ALOA SP 37MC4901
0.1883,000ALOA SP 37MC4918
0.3293,000ALOA SP 37MC4968
-0.4251,500PL SP 37MC5510
-0.7340,000ALOA SP 37MC5517
-0.2360,000ALOA SP 37MC5528
-0.9334,000ALOA SP 37MC5566
-0.5347,000OtherMC5566
-0.5347,000PL SP 37MC5566
-0.0370,000OtherMC5666
-0.3156,000ALOA SP 37MC5755
0.2185,000OtherMC5774
-0.2758,000ALOA SP 37MC5888
0.60117,000ALOA SP 37MC6060
0.1682,000ALOA SP 37MC6215
-0.1763,000OtherMC6278
-0.2758,000ALOA SP 37MC6353
-0.9533,400OtherMC6366
-0.5048,000OtherMC6394
-0.3753,333ALOA SP 37MC6514
0.44103,000ALOA SP 37MC6526
0.54111,000ALOA SP 37MC6564
0.73130,000PL SP 37MC6722
0.3394,000OtherMC6809
0.3092,000ALOA SP 37MC6809
0.0374,000ALOA SP 37MC6948
0.1380,000ALOA SP 37MC7135
0.83140,000PL SP 37MC7233
-0.3156,000ALOA SP 37MC7255
-0.1564,000ALOA SP 37MC7352
Page 30 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria monocytogenes
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.8835,500OtherMC7428
-0.7340,000ALOA SP 37MC7428
0.63120,000ALOA SP 37MC7560
0.2387,000ALOA SP 37MC7583
0.2890,000ALOA SP 37MC8164
-2.847,273ALOA SP 37MC8170
-0.4849,000OtherMC8240
0.3797,000ALOA SP 37MC8949
-0.2161,000OtherMC8981
0.2286,000OtherMC9005
-1.1029,637OtherMC9068
-1.0930,000OtherMC9086
>1,000OtherMC9206
0.1380,000ALOA SP 37MC9282
-1.0930,000ALOA SP 37MC9473
Value
Data Statistics
137Number of Results
2Number of Excluded Results
4.81 log10Mean
4.86 log10Median
0.20 log10Standard Deviation
0.16 log10Robust Standard Deviation
7273 to 140000 cfu/gResult Range
Performance Statistics
Value
72,000 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
14,366 to 360,855 cfu/gSatisfactory Range
98.5%Satisfactory z scores
1.5%Questionable z scores
0.0%Unsatisfactory z scores
Page 31 of 123Issue: 1 09 October 2018
Sample: 08F - Listeria (Enumeration)
Analyte: Listeria monocytogenes
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
98.77273 to120000
0.154.8757.66179ALOA SP 37
87.57700 to140000
0.234.855.8408PL SP 37
100.029637 to140000
0.184.8136.5150Other
98.57273 to140000
0.164.861002137All
Comments
The sample contained Listeria monocytogenes at an assigned value of 72,000 cfu/g.
There are ten species of Listeria, the most clinically significant being Listeria monocytogenes, which is pathogenic for humans and animals.While other members of the Listeria genus are not usually implicated in disease, their presence indicates an increased risk of contaminationby Listeria monocytogenes.
Page 32 of 123Issue: 1 09 October 2018
Sample: 09D - Enterococci (faecal streptococci)
Analyte: Enterococci (faecal streptococci)
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.031,265SB SP 37MC0063
0.091,400SB SP 37MC0302
-0.101,200OtherMC0309
0.091,400OtherMC0310
-0.77700OtherMC0390
0.001,300KA SP 37MC0495
0.001,300KF SP 37MC0495
-0.151,150KF PP 37MC0729
0.381,768KF PP 37MC0752
0.822,520KF SP 37MC0914
0.241,580KF PP 37MC0963
-0.60800OtherMC1059
-0.211,100KA SP 37MC1094
0.331,700OtherMC1354
-1.29460KA SP 37MC1579
0.161,480KA SP 37MC1580
0.261,600OtherMC1923
-0.56829KF SP 37MC1980
0.071,375OtherMC2061
0.091,400KF PP 37MC2490
0.532,000KF SP 37MC2620
-0.62790KA SP 37MC2951
-2.00260KA SP 37MC3003
-1.32450KA SP 37MC3014
-0.39950SB SP 37MC3014
-5.7113KA SP 37MC3015
0.031,327OtherMC3173
0.001,300OtherMC3249
0.181,500OtherMC3269
-0.031,265KA SP 37MC3404
0.401,800OtherMC3443
-0.44910KA SP 37MC3486
-0.46900KA SP 37MC3624
-0.68750OtherMC4716
2.258,000KF PP 37MC4755
-0.42930OtherMC4836
0.261,600KF SP 37MC5690
0.451,863OtherMC5824
-0.60800SB SP 37MC6246
0.101,410OtherMC6251
0.261,600SB SP 37MC6406
0.091,400OtherMC6446
-0.151,150SB SP 37MC6447
-0.35980OtherMC6651
-0.101,200OtherMC6723
-0.211,100KF PP 37MC7238
-1.02570KA SP 37MC7279
0.141,460KF PP 37MC7332
0.652,200OtherMC7337
-0.38960KA SP 37MC7775
-0.141,160KA SP 37MC7956
0.001,300KF PP 37MC7957
0.331,700KF PP 37MC9406
Page 33 of 123Issue: 1 09 October 2018
Sample: 09D - Enterococci (faecal streptococci)
Analyte: Enterococci (faecal streptococci)
Round: 269Scheme: Food Microbiology (QMS)
Value
Data Statistics
53Number of Results
1Number of Excluded Results
3.08 log10Mean
3.11 log10Median
0.21 log10Standard Deviation
0.13 log10Robust Standard Deviation
260 to 8000 cfu/gResult Range
Performance Statistics
Value
1,300 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
259 to 6,515 cfu/gSatisfactory Range
96.2%Satisfactory z scores
1.9%Questionable z scores
1.9%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.0829 to 25200.143.209.4305KF SP 37
88.91100 to 80000.103.1616.9809KF PP 37
92.9260 to 14800.212.9626.42114KA SP 37
100.0800 to 16000.123.0811.3206SB SP 37
100.0700 to 22000.103.1435.85019Other
96.2260 to 80000.133.11100153All
Comments
Page 34 of 123Issue: 1 09 October 2018
Sample: 09D - Enterococci (faecal streptococci)
Analyte: Enterococci (faecal streptococci)
Round: 269Scheme: Food Microbiology (QMS)
The sample contained Enterococcus faecalis at an assigned value of 1,300 cfu/g.
Enterococci are widely distributed in the environment and are normal commensals of the intestinal tracts of animals, birds and humans. Theorganisms survive well in adverse conditions, and are resistant to heat, freezing, desiccation and chemicals, such as chlorine. For thisreason Enterococci are commonly used as indicator organisms, particularly in dried and frozen foods and also potable water samples.
Page 35 of 123Issue: 1 09 October 2018
Analyte: Clostridium species
Sample: 10F - Clostridium
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedBroth/TSC 37MC0212
DetectedBroth/TSC 37MC0302
DetectedOtherMC1467
DetectedOtherMC1727
DetectedBroth/TSC 37MC1764
DetectedBroth/TSC 37MC1926
DetectedOtherMC2163
DetectedBroth/TSC 37MC2345
DetectedBroth/TSC 37MC2364
DetectedOtherMC2510
DetectedOtherMC2612
DetectedOtherMC2824
DetectedBroth/TSC 37MC3492
DetectedOtherMC3583
DetectedOtherMC3647
DetectedOtherMC4067
DetectedOtherMC4843
DetectedOtherMC4851
DetectedBroth/TSC 37MC4852
DetectedOtherMC4901
DetectedBroth/TSC 37MC4975
DetectedOtherMC5600
DetectedOtherMC6292
DetectedRCA PP 37MC6722
DetectedBroth/TSC 37MC7057
DetectedRCA PP 37MC7057
DetectedRCA PP 37MC7365
Result
Data Statistics
DetectedAssigned Value
27Number of Results
100%Satisfactory
Methodology Summary
% SatisfactoryMethod
100%Broth/TSC 37
100%RCA PP 37
100%Other
Comments
The sample contained Clostridium perfringens at an approximate level of 4,400 cfu/g. The assigned value is therefore Clostridium species ‘detected’.
Clostridium is a large genus consisting of Gram-positive spore-forming anaerobes; they are usually straight rods which are catalasenegative. Some species are strict anaerobes, and some are aerotolerant. Clostridia are often found in soil, some are responsible for humanand animal disease, and others are associated with food spoilage. Clostridia are classified according to the shape and position of theirspores, and by their physiological characteristics.
Issue: 1 Page 36 of 123 09 October 2018
Sample: 10F - Clostridium
Analyte: Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.363,300TSC PP 37MC0159
0.154,950TSC PP 37MC0212
0.215,200TSC PP 37MC0302
-0.094,100TSC PP 37MC0379
0.566,900OPSP PP 37MC0575
0.114,800TSC PP 37MC0738
1.019,900OPSP PP 37MC1008
<10TSC PP 37MC1065
-0.483,000OtherMC1139
0.285,500OtherMC1467
0.064,600TSC PP 37MC1538
0.506,600TSC PP 37MC1727
<10TSC PP 37MC1764
-0.652,600TSC PP 37MC1849
-0.064,200TSC PP 37MC2163
0.255,400TSC PP 37MC2345
0.737,900TSC PP 37MC2364
-0.253,600TSC PP 37MC2388
0.034,500TSC PP 37MC2409
0.034,500TSC PP 37MC2468
-0.483,000OtherMC2588
0.134,900TSC PP 37MC2612
0.165,000TSC PP 37MC2647
1.5215,000OtherMC2824
0.587,000TSC PP 37MC2834
0.737,900TSC PP 37MC3002
-0.034,300TSC PP 37MC3003
-0.283,500TSC PP 37MC3014
-0.682,550OtherMC3014
-0.483,000TSC PP 37MC3102
-0.652,600OtherMC3243
-0.433,100OtherMC3246
-0.064,200OtherMC3249
0.084,700OtherMC3250
-0.034,300OtherMC3251
0.004,400OtherMC3253
-0.922,100TSC PP 37MC3399
0.285,500TSC PP 37MC3423
-0.323,400TSC PP 37MC3477
-0.064,200TSC PP 37MC3492
-0.323,400TSC PP 37MC3519
0.165,000TSC PP 37MC3576
-0.672,569TSC PP 37MC3583
0.215,200TSC PP 37MC3591
0.205,182TSC PP 37MC3638
0.185,100TSC PP 37MC3647
0.909,100OtherMC3973
0.436,200TSC PP 37MC4067
-0.483,000OtherMC4262
-0.223,700TSC PP 37MC4296
-2.08820TSC PP 37MC4356
0.386,000TSC PP 37MC4843
0.909,100TSC PP 37MC4851
0.365,900TSC PP 37MC4852
-0.403,200TSC PP 37MC4870
0.486,500TSC PP 37MC4901
-0.064,200TSC PP 37MC4918
-0.802,300TSC PP 37MC4975
-0.483,000OPSP PP 37MC5600
0.004,400TSC PP 37MC5657
1.1411,000OtherMC5742
Page 37 of 123Issue: 1 09 October 2018
Sample: 10F - Clostridium
Analyte: Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.223,700OtherMC5802
0.084,700TSC PP 37MC5937
-0.363,300TSC PP 37MC5939
-0.433,100TSC PP 37MC5940
-0.064,200OtherMC6292
-0.283,500OPSP PP 37MC6380
0.255,400TSC PP 37MC6457
-0.253,600TSC PP 37MC6526
0.637,300TSC PP 37MC6667
-0.802,300TSC PP 37MC6722
-0.433,100OPSP PP 37MC6948
0.235,300TSC PP 37MC6995
-0.752,400TSC PP 37MC7057
-0.862,200IS PP 37MC7057
0.165,000TSC PP 37MC7167
-3.21330OtherMC7419
0.778,200TSC PP 37MC7461
0.526,700TSC PP 37MC7591
-0.702,500TSC PP 37MC7955
-2.83450TSC PP 37MC8196
0.034,500TSC PP 37MC8231
0.034,500TSC PP 37MC8291
1.0210,000OtherMC9360
Value
Data Statistics
84Number of Results
2Number of Excluded Results
3.62 log10Mean
3.64 log10Median
0.25 log10Standard Deviation
0.19 log10Robust Standard Deviation
330 to 15000 cfu/gResult Range
Performance Statistics
Value
4,400 cfu/gAssigned Value
0.03 log10Uncertainty of Assigned Value
0.35 log10SDPA
878 to 22,052 cfu/gSatisfactory Range
96.3%Satisfactory z scores
2.4%Questionable z scores
1.2%Unsatisfactory z scores
Page 38 of 123Issue: 1 09 October 2018
Sample: 10F - Clostridium
Analyte: Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
96.6450 to 91000.153.6571.43260TSC PP 37
100.03000 to 99000.103.545.9505OPSP PP 37
100.02200 to 22000.003.341.1901IS PP 37
94.4330 to 150000.223.6221.43018Other
96.3330 to 150000.193.64100284All
Comments
The sample contained Clostridium perfringens at an assigned value of 4,400 cfu/g.
Clostridium perfringens causes an intoxication form of food poisoning characterised by diarrhoea, stomach cramps, nausea, headache, andfever. Foods most commonly associated with Clostridium perfringens contamination are usually high protein foods such as meat and poultrystews, sauces, gravies, pies, and casseroles.
Page 39 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.62140,000PCA PP 30MC0051
0.0891,000PetrifilmMC0068
-0.4360,000PCA PP 30MC0122
0.20100,000PCA PP 30MC0192
0.55132,500PCA PP 30MC0249
-0.1277,000PCA PP 30MC0261
0.66145,000PCA PP 30MC0676
-1.2531,000MPCA PP 30MC0888
0.31109,000PetrifilmMC0965
0.32110,000PetrifilmMC1033
-0.8543,000PetrifilmMC1047
-0.2868,000PCA PP 30MC1073
-0.2172,000PetrifilmMC1075
0.32110,000PetrifilmMC1082
-0.5256,000PetrifilmMC1085
0.28106,500PCA PP 30MC1093
-0.7048,500PetrifilmMC1261
2.04440,000PCA PP 30MC1283
0.35113,000MPCA PP 30MC1354
0.1193,100OtherMC1354
-0.3763,000PCA PP 30MC1611
0.56134,000OtherMC1651
0.1596,000PetrifilmMC1651
0.1797,273PCA PP 30MC1710
0.0286,636PCA PP 30MC1712
0.21100,909PCA PP 30MC1713
0.1696,364PCA PP 30MC1836
0.78160,000PetrifilmMC1862
-0.0979,000MPCA PP 30MC1865
-0.0383,000OtherMC1865
0.0689,499OtherMC1918
0.27106,000PCA PP 30MC1923
0.53130,000PCA PP 30MC1939
0.0085,000PCA PP 30MC2042
0.24103,000MPCA PP 30MC2061
0.86170,000PCA PP 30MC2095
0.43120,000OtherMC2154
0.22101,818PCA PP 30MC2176
0.0185,700PCA PP 30MC2282
-0.2868,000PCA PP 37MC2413
-0.9041,216MPCA PP 30MC2436
0.62140,000PCA PP 30MC2479
-0.3166,000MPCA PP 30MC2490
0.27105,500MPCA PP 30MC2580
0.24103,000PetrifilmMC2581
0.30108,000OtherMC2668
-0.3365,000PCA PP 30MC2696
0.1495,000PCA PP 30MC2737
-0.0383,000PCA PP 30MC2884
-0.6650,000PetrifilmMC2930
-0.9041,000PCA PP 30MC3000
0.49126,000MPCA PP 30MC3001
-8.31105MPCA PP 30MC3015
-1.0935,333PCA PP 30MC3038
-0.8244,000MPCA PP 30MC3120
-0.7646,000PCA SP 30MC3120
0.0186,000PetrifilmMC3172
0.70148,917PCA PP 30MC3334
-0.3763,330MPCA PP 30MC3367
3.171,090,000PCA PP 30MC3443
3.341,250,000PetrifilmMC3443
Page 40 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-1.7520,700PCA PP 30MC3478
-0.1476,000PCA PP 30MC3499
0.74154,000MPCA PP 30MC3517
-1.1533,544PCA PP 30MC3583
0.1092,000PCA PP 30MC3624
0.32110,000OtherMC3782
-0.4658,700PetrifilmMC3795
0.34111,500PCA PP 30MC3911
0.1596,000MPCA PP 30MC3921
0.25104,333PetrifilmMC3967
-0.7447,000PetrifilmMC4034
-0.1973,000PCA PP 37MC4077
-0.1376,605PCA SP 30MC4424
1.00190,000PetrifilmMC4458
-0.2072,500OtherMC4485
-0.4559,000PCA PP 30MC4489
2.97930,000PCA PP 30MC4695
0.1495,000OtherMC4725
0.1092,000PCA PP 30MC4725
-0.2271,000PCA PP 30MC4772
-0.4758,000PetrifilmMC4838
0.1696,364PCA PP 30MC4853
0.32110,000PCA SP 30MC4943
0.20100,000MPCA PP 30MC5008
0.23102,000PetrifilmMC5008
-0.7546,500MPCA PP 30MC5055
-0.1178,000PCA PP 30MC5531
0.1193,000MPCA PP 30MC5657
-0.0681,000PetrifilmMC5709
0.75155,000OtherMC5723
-0.2470,220MPCA PP 30MC5769
-0.0880,000PCA PP 30MC5809
0.21101,000PCA PP 30MC5815
-0.2768,180PCA PP 30MC5824
0.60138,000PCA PP 30MC6366
-0.0681,000PCA PP 37MC6517
-3.405,500MPCA PP 30MC6671
-0.1476,133PCA SP 30MC6709
0.0890,700PCA PP 30MC6717
-0.1476,000OtherMC6927
0.1696,500PCA PP 30MC6951
0.0085,000PetrifilmMC6993
-2.868,500PetrifilmMC7052
0.24103,000PCA PP 37MC7057
0.1697,000PCA PP 30MC7057
0.33111,000OtherMC7057
-0.1575,380PCA PP 30MC7193
-0.1476,000PCA SP 30MC7197
-2.878,420PCA PP 30MC7199
-0.9639,320OtherMC7296
-0.7945,000PCA PP 30MC7369
-0.2172,000PCA PP 37MC7371
-0.0879,500PCA SP 30MC7468
0.0085,000PCA PP 37MC7574
-0.2470,000MPCA PP 30MC7621
-0.3763,000PCA PP 30MC7658
0.55132,200PCA PP 30MC7723
-1.2930,000PCA PP 30MC7745
-1.3828,000PCA PP 37MC7745
-3.674,400PCA PP 30MC7786
-0.2470,000OtherMC7787
Page 41 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.21101,000PCA PP 30MC7957
0.1495,000PCA PP 37MC8024
-0.4360,100PCA PP 30MC8029
0.47124,000PetrifilmMC8048
-0.0681,000OtherMC8063
-0.1178,000PCA PP 30MC8065
0.0790,000PCA PP 30MC8228
-0.4758,000PCA SP 30MC8395
0.0891,000PetrifilmMC8834
-0.9440,000PCA PP 30MC8837
0.50127,000PetrifilmMC8843
-0.3365,000PetrifilmMC8844
-0.0482,000PetrifilmMC8845
0.45122,000PetrifilmMC8846
-0.1178,000PetrifilmMC8847
0.0891,000PetrifilmMC8848
0.44121,000PetrifilmMC8849
-0.5057,000PetrifilmMC8979
-0.3962,000PetrifilmMC9064
0.44120,750PetrifilmMC9098
-0.4360,000PetrifilmMC9102
-0.2669,110PetrifilmMC9244
-0.3564,000PetrifilmMC9250
0.43120,000PetrifilmMC9252
-0.2271,000PetrifilmMC9341
0.30108,000PetrifilmMC9382
-0.3166,000OtherMC9398
0.43120,000OtherMC9468
0.20100,000PetrifilmMC9545
0.0488,000PetrifilmMC9560
-0.4360,000PCA PP 37MC9574
-0.4360,000OtherMC9574
<10PetrifilmMC9580
-0.1277,000PetrifilmMC9581
Value
Data Statistics
156Number of Results
2Number of Excluded Results
4.91 log10Mean
4.93 log10Median
0.30 log10Standard Deviation
0.16 log10Robust Standard Deviation
4400 to 1250000 cfu/gResult Range
Performance Statistics
Value
85,000 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
16,960 to 426,009 cfu/gSatisfactory Range
94.2%Satisfactory z scores
2.6%Questionable z scores
3.2%Unsatisfactory z scores
Page 42 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
91.44400 to1090000
0.174.9637.18058PCA PP 30
100.046000 to110000
0.034.884.4907PCA SP 30
100.028000 to103000
0.104.865.7709PCA PP 37
89.55500 to154000
0.224.8712.18119MPCA PP 30
95.58500 to1250000
0.174.9428.85145Petrifilm
100.039320 to155000
0.144.9611.54018Other
94.24400 to1250000
0.164.931002156All
Comments
The sample contained Bacillus subtilis and Escherichia coli at an assigned value of 85,000 cfu/g.
Total aerobic mesophilic counts are used to determine the general microbial population of a sample.
Page 43 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.63110,000VRBA PP 37MC0051
0.1776,000PetrifilmMC0068
0.72118,500PetrifilmMC0249
>5,000COLI ID PP 37MC0298
0.4293,000PetrifilmMC1033
-0.4546,000VRBA PP 37MC1073
-0.1758,000PetrifilmMC1075
0.3286,000PetrifilmMC1082
0.2581,000PetrifilmMC1085
0.64111,500VRBA PP 30MC1093
-3.494,000PetrifilmMC1261
-0.5443,000VRBA PP 37MC1283
0.4293,000VRBA PP 30MC1354
0.63110,000MPNMC1710
0.0770,000OtherMC1710
0.4091,818VRBA PP 30MC1712
0.0870,909VRBA PP 30MC1713
0.1675,455VRBA PP 30MC1836
-0.6639,000VRBA PP 30MC1865
-0.8334,000OtherMC1865
0.2480,499OtherMC1918
-0.0862,000VRBA PP 30MC1923
0.73120,000OtherMC1939
0.0569,000VRBA PP 37MC2042
0.4898,000VRBA PP 30MC2061
0.79125,000VRBA PP 37MC2095
0.0066,364VRBA PP 30MC2176
-0.4546,000MPNMC2282
0.2279,000VRBA PP 30MC2479
-0.2156,000VRBA PP 37MC2490
0.0770,000VRBA PP 37MC2580
-0.9930,000PetrifilmMC2696
-0.6938,000VRBA PP 37MC2696
-0.7237,000OtherMC2884
-0.7935,000VRBA PP 37MC3000
-0.5144,000VRBA PP 30MC3000
-0.5941,100COLI ID PP 37MC3001
-8.4275VRBA PP 37MC3015
-0.3052,000PetrifilmMC3172
-0.0862,000VRBA PP 30MC3443
-0.3351,000PetrifilmMC3443
0.51100,000VRBA PP 30MC3478
-0.3052,000VRBA PP 30MC3499
-1.0029,611VRBA PP 37MC3583
0.3689,000VRBA PP 30MC3782
<10PetrifilmMC3795
-0.9431,000VRBA PP 30MC4034
0.1474,000VRBA PP 37MC4077
0.1776,393Chromogenic agarMC4424
0.0569,000PetrifilmMC4458
0.4091,700PetrifilmMC4485
-0.9431,000VRBA PP 37MC4489
3.09800,000VRBA PP 30MC4695
0.4293,000MPNMC4725
0.1072,000PetrifilmMC4725
0.0066,364VRBA PP 37MC4853
-0.1957,000VRBA PP 37MC4943
-1.0728,000VRBA PP 30MC5055
0.1474,000VRBA PP 30MC5657
-0.6340,000VRBA PP 37MC5809
-0.1061,000VRBA PP 37MC5815
Page 44 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.0464,500VRBA PP 30MC5824
8.4560,000,000VRBA PP 30MC6240
0.75121,000Chromogenic agarMC6366
-0.3849,000VRBA PP 37MC6517
-4.701,500MPNMC6709
-0.0663,000VRBA PP 30MC6717
0.1575,000OtherMC6927
0.1072,000COLI ID PP 37MC6993
-2.598,200OtherMC7052
0.1474,000VRBA PP 37MC7057
0.2380,000Chromogenic agarMC7057
0.3487,000OtherMC7057
-0.1459,500OtherMC7193
0.1575,000OtherMC7197
-3.334,550PetrifilmMC7199
-0.3848,700OtherMC7296
<10PetrifilmMC7369
-0.2853,000PetrifilmMC7468
0.2279,000COLI ID PP 37MC7574
-0.3052,000VRBA PP 37MC7621
-0.9132,000VRBA PP 30MC7658
0.3689,000PetrifilmMC7723
-3.553,800VRBA PP 37MC7786
-0.1957,000OtherMC7787
-0.2853,000VRBA PP 37MC8024
-0.5443,000VRBA PP 37MC8029
0.3689,000PetrifilmMC8048
0.3286,000OtherMC8228
-0.4048,000VRBA PP 30MC8395
0.3991,000PetrifilmMC8834
-0.0663,000PetrifilmMC8843
-0.2355,000PetrifilmMC8844
-0.1061,000PetrifilmMC8845
0.3286,000PetrifilmMC8846
-0.2654,000PetrifilmMC8847
0.3487,000PetrifilmMC8848
0.1072,000PetrifilmMC8849
0.1776,000PetrifilmMC8979
-3.843,000PetrifilmMC9018
-0.0862,400PetrifilmMC9064
0.3588,000PetrifilmMC9098
<10PetrifilmMC9102
0.55103,000PetrifilmMC9150
0.67113,500PetrifilmMC9151
0.3185,000PetrifilmMC9152
-0.1957,110PetrifilmMC9244
-0.4845,000PetrifilmMC9250
0.1474,000PetrifilmMC9252
-0.3550,000PetrifilmMC9341
-1.1626,000PetrifilmMC9382
1.01150,000OtherMC9468
0.3890,000PetrifilmMC9545
0.0871,000PetrifilmMC9560
-0.1360,000OtherMC9574
<10PetrifilmMC9580
-0.3052,000PetrifilmMC9581
Page 45 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
Value
Data Statistics
117Number of Results
7Number of Excluded Results
4.75 log10Mean
4.82 log10Median
0.35 log10Standard Deviation
0.17 log10Robust Standard Deviation
1500 to 800000 cfu/gResult Range
Performance Statistics
Value
66,364 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
13,241 to 332,608 cfu/gSatisfactory Range
92.0%Satisfactory z scores
0.9%Questionable z scores
7.1%Unsatisfactory z scores
Page 46 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
91.33800 to125000
0.184.7219.66123VRBA PP 37
91.728000 to800000
0.214.8220.51124VRBA PP 30
100.041100 to79000
0.064.863.4214COLI ID PP 37
100.076393 to121000
0.034.902.5603Chromogenicagar
75.01500 to110000
0.284.823.4204MPN
92.53000 to118500
0.154.8537.61444Petrifilm
93.38200 to150000
0.134.8512.82015Other
92.01500 to800000
0.174.821007117All
Comments
The sample contained Escherichia coli at an assigned value of 66,364 cfu/g.
Coliforms are found in human and animal intestines, and their presence in foods is used as an indicator of faecal contamination. However,many coliforms are also found throughout the environment, being isolated from air, soil, water, plants, and animals. The presence ofcoliform indicator organisms in foods may, therefore, be indicative of faecal contamination, but may also be environmental in origin.
Page 47 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.70110,000VRBGA PP 37MC0051
-0.3846,000PetrifilmMC0068
0.0465,000VRBGA PP 37MC0122
-0.4145,000VRBGA PP 37MC0192
0.2576,500VRBGA PP 37MC0261
0.64105,000VRBGA PP 37MC0676
-2.419,000VRBGA PP 37MC0888
0.1470,000PetrifilmMC0965
-0.4743,100PetrifilmMC1047
-0.2850,000VRBGA PP 37MC1073
-0.3348,000PetrifilmMC1075
0.4288,000PetrifilmMC1082
0.90129,000VRBGA PP 37MC1093
-0.5042,000VRBGA PP 37MC1283
0.2476,000VRBGA PP 37MC1354
0.3885,000OtherMC1651
0.2275,000PetrifilmMC1651
0.1470,000VRBGA PP 37MC1710
0.0565,455VRBGA PP 37MC1712
0.4489,091VRBGA PP 37MC1713
0.4590,000VRBGA PP 37MC1836
0.2778,000PetrifilmMC1862
0.0364,000PetrifilmMC1865
-0.1456,000OtherMC1865
0.2275,000VRBGA PP 37MC1923
0.81120,000OtherMC1939
0.1470,000VRBGA PP 37MC2042
0.3382,000VRBGA PP 37MC2061
0.5496,500OtherMC2154
0.1068,182VRBGA PP 37MC2176
0.2577,000VRBGA PP 30MC2282
-0.2153,000VRBGA PP 30MC2413
-0.2252,400VRBGA PP 30MC2436
0.70110,000VRBGA PP 30MC2479
0.1571,000VRBGA PP 37MC2490
0.5698,500VRBGA PP 37MC2580
-0.7235,000PetrifilmMC2696
-0.5341,000VRBGA PP 37MC2696
0.3381,500VRBGA PP 37MC2737
-0.1257,000PetrifilmMC2930
-0.2352,000VRBGA PP 37MC3000
-0.3846,000VRBGA PP 30MC3000
-0.2850,000OtherMC3000
-0.4942,400VRBGA PP 30MC3001
-8.4271VRBGA PP 37MC3015
-1.1724,454VRBGA PP 37MC3038
-0.2153,000VRBGA PP 30MC3120
-0.2352,000VRBGA PP 37MC3120
-0.2352,000PetrifilmMC3172
0.5698,667PetrifilmMC3334
-0.5341,000VRBGA PP 37MC3367
-0.5839,200VRBGA PP 30MC3367
0.4993,000PetrifilmMC3443
0.2174,000VRBGA PP 37MC3443
0.74114,000VRBGA PP 37MC3478
-0.1954,000VRBGA PP 30MC3499
-0.6337,600VRBGA PP 37MC3517
-1.0726,447VRBGA PP 37MC3583
-0.1058,000VRBGA PP 37MC3624
0.2476,000OtherMC3782
-0.6537,000PetrifilmMC3811
Page 48 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.63104,500PetrifilmMC3911
0.1872,500VRBGA PP 30MC3911
-0.6238,000VRBGA PP 37MC3921
-0.2650,666PetrifilmMC3967
-0.6936,000VRBGA PP 37MC4034
0.2476,000VRBGA PP 37MC4077
0.0565,136VRBGA PP 30MC4424
-0.0859,000PetrifilmMC4485
-1.0028,000VRBGA PP 37MC4489
0.3382,000PetrifilmMC4725
-0.1257,000PetrifilmMC4772
-0.0859,000PetrifilmMC4838
0.1068,182VRBGA PP 37MC4853
0.2174,000VRBGA PP 37MC4943
0.2275,000PetrifilmMC5008
0.3281,000VRBGA PP 37MC5008
-0.5341,000VRBGA PP 37MC5055
0.3583,000VRBGA PP 37MC5531
0.1571,000VRBGA PP 30MC5657
0.3684,000PetrifilmMC5709
0.3180,320VRBGA PP 37MC5769
-0.2451,820VRBGA PP 37MC5809
0.4892,000VRBGA PP 37MC5815
-0.0361,000VRBGA PP 37MC5824
-0.0460,666PetrifilmMC5889
0.99139,000VRBGA PP 37MC6366
-0.0759,500VRBGA PP 37MC6517
-4.551,600VRBGA PP 37MC6671
-0.1854,100VRBGA PP 37MC6709
0.0062,700VRBGA PP 37MC6717
0.2577,000PetrifilmMC6927
0.2577,000VRBGA PP 37MC6951
0.1571,000VRBGA PP 37MC6993
0.5295,000VRBGA PP 37MC7057
0.4590,000PetrifilmMC7057
0.2778,000OtherMC7057
-0.0659,860VRBGA PP 37MC7193
-0.1257,000VRBGA PP 37MC7197
-3.603,450PetrifilmMC7199
-0.2252,716OtherMC7296
-0.9130,000VRBGA PP 37MC7369
-0.1655,000VRBGA PP 37MC7468
-0.2352,000VRBGA PP 37MC7621
-0.6936,000VRBGA PP 37MC7658
0.5295,500PetrifilmMC7723
0.1973,000VRBGA PP 37MC7745
-3.354,200PetrifilmMC7786
-0.2153,000OtherMC7787
0.58100,000VRBGA PP 37MC7957
-0.2651,000VRBGA PP 37MC8029
0.1470,000PetrifilmMC8048
-0.0162,000OtherMC8063
0.2577,000VRBGA PP 37MC8065
0.3583,000VRBGA PP 37MC8228
-0.2153,000VRBGA PP 30MC8395
-0.5640,000VRBGA PP 37MC8837
0.4892,000PetrifilmMC8846
-3.145,000PetrifilmMC9018
0.2979,000PetrifilmMC9252
-0.8033,000PetrifilmMC9382
-1.0926,000PetrifilmMC9545
Page 49 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.1973,000PetrifilmMC9560
-2.1611,000PetrifilmMC9570
-1.7715,000VRBGA PP 37MC9570
-0.1954,000PetrifilmMC9581
Value
Data Statistics
126Number of Results
1Number of Excluded Results
4.74 log10Mean
4.80 log10Median
0.29 log10Standard Deviation
0.15 log10Robust Standard Deviation
1600 to 139000 cfu/gResult Range
Performance Statistics
Value
62,700 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
12,510 to 314,244 cfu/gSatisfactory Range
94.4%Satisfactory z scores
1.6%Questionable z scores
4.0%Unsatisfactory z scores
Page 50 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
95.51600 to139000
0.154.8252.38166VRBGA PP 37
100.039200 to110000
0.134.7210.32013VRBGA PP 30
89.23450 to104500
0.184.7829.37037Petrifilm
100.050000 to120000
0.154.847.94010Other
94.41600 to139000
0.154.801001126All
Comments
The sample contained Escherichia coli at an assigned value of 62,700 cfu/g.
Members of the Enterobacteriaceae family are distributed worldwide and found in soil, water, fruits, vegetables, grains, flowering plants, anda wide range of animals, from insects to humans.
Page 51 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-10.8011MPNMC0051
0.1776,000PetrifilmMC0068
-0.0564,000TBX PP 37-44MC0192
0.2984,000PetrifilmMC0249
-0.0962,000PetrifilmMC0261
>5,000COLI ID PP 37-44MC0298
0.0569,000TBX PP 37-44MC0676
0.4293,000PetrifilmMC1033
-0.2853,000TBX PP 37-44MC1073
0.3890,000PetrifilmMC1082
-0.0763,000PetrifilmMC1085
-1.4321,000TBX PP 37-44MC1283
0.2078,000Chromogenic agarMC1354
0.3387,000TBX PP 37-44MC1354
-0.4646,000MPNMC1710
0.0167,273TBX PP 37-44MC1710
-0.0464,545TBX PP 37-44MC1712
0.62110,000MPNMC1713
0.0870,909TBX PP 37-44MC1713
-0.0563,636TBX PP 37-44MC1836
0.62110,000MPNMC1836
0.0368,000PetrifilmMC1865
-0.1360,000OtherMC1865
0.73120,000OtherMC1939
0.0468,700Chromogenic agarMC2042
0.0368,000TBX PP 37-44MC2061
0.2481,000OtherMC2154
0.62110,000MPNMC2176
-0.1160,909TBX PP 37-44MC2176
-0.3152,000TBX PP 37-44MC2282
0.62110,000TBX PP 37-44MC2479
-0.4048,000TBX PP 37-44MC2490
-0.5144,000TBX PP 37-44MC2696
-0.6340,000PetrifilmMC2696
-0.1957,000TBX PP 37-44MC3000
<10COLI ID PP 37-44MC3001
-9.2040TBX PP 37-44MC3015
0.2179,000PetrifilmMC3172
0.55104,000PetrifilmMC3443
0.68115,000Chromogenic agarMC3478
-0.2654,000TBX PP 37-44MC3499
-1.0229,317Chromogenic agarMC3583
-0.2654,000TBX PP 37-44MC3624
0.3689,000TBX PP 37-44MC3782
0.0871,000PetrifilmMC3795
-0.8035,000PetrifilmMC3811
0.53102,000PetrifilmMC3911
0.0368,000OtherMC4034
0.0167,000TBX PP 37-44MC4077
0.1776,393Chromogenic agarMC4424
-0.5144,000TBX PP 37-44MC4489
0.4293,000MPNMC4725
0.1072,000PetrifilmMC4725
-0.0663,363TBX PP 37-44MC4853
-0.0564,091TBX PP 37-44MC4892
-0.8334,000OtherMC4943
-0.8932,500TBX PP 37-44MC5055
-0.3151,800TBX PP 37-44MC5531
-0.4845,000OtherMC5657
0.4797,000PetrifilmMC5723
0.0669,520TBX PP 37-44MC5769
Page 52 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.1776,400TBX PP 37-44MC5809
0.3689,000TBX PP 37-44MC5824
0.51100,000TBX PP 37-44MC6240
0.74121,000Chromogenic agarMC6366
-0.3849,000VRBA PP 37-44MC6517
0.1575,000MPNMC6709
0.1072,300TBX PP 37-44MC6717
0.1776,000OtherMC6927
0.1072,000COLI ID PP 37-44MC6993
-2.608,200OtherMC7052
0.1072,000TBX PP 37-44MC7057
-0.1360,000Chromogenic agarMC7057
0.2481,000OtherMC7057
-0.2056,800TBX PP 37-44MC7193
-0.4646,000PetrifilmMC7197
-3.374,410PetrifilmMC7199
-0.6340,000PetrifilmMC7369
-0.4347,000PetrifilmMC7468
-0.3152,000OtherMC7621
-0.4845,000PetrifilmMC7658
0.53102,000TBX PP 37-44MC7723
-3.165,200PetrifilmMC7786
-0.0365,000OtherMC7787
0.3689,000PetrifilmMC8048
-0.3550,000OtherMC8063
-0.4247,500TBX PP 37-44MC8065
0.2883,000TBX PP 37-44MC8228
-0.8733,000Chromogenic agarMC8395
-0.1161,000PetrifilmMC8845
0.3085,000PetrifilmMC8846
-0.2654,000PetrifilmMC8847
0.3387,000PetrifilmMC8848
-0.0166,000PetrifilmMC8979
-4.701,500PetrifilmMC9018
0.3588,000PetrifilmMC9098
-0.2455,000PetrifilmMC9102
-0.6140,600PetrifilmMC9244
-0.6938,000PetrifilmMC9250
0.1877,000PetrifilmMC9252
-0.3550,000PetrifilmMC9341
0.62110,000OtherMC9468
0.2078,000PetrifilmMC9545
0.1575,000PetrifilmMC9560
-0.1360,000OtherMC9574
<10PetrifilmMC9580
-0.3550,000PetrifilmMC9581
Value
Data Statistics
107Number of Results
5Number of Excluded Results
4.77 log10Mean
4.82 log10Median
0.28 log10Standard Deviation
0.16 log10Robust Standard Deviation
1500 to 121000 cfu/gResult Range
Page 53 of 123Issue: 1 09 October 2018
The sample contained Escherichia coli at an assigned value of 66,498 cfu/g.
Sample: 16D - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
Performance Statistics
Value
66,498 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
13,268 to 333,279 cfu/gSatisfactory Range
94.2%Satisfactory z scores
1.0%Questionable z scores
4.8%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
97.221000 to110000
0.114.8133.64136TBX PP 37-44
100.029317 to121000
0.214.867.4808Chromogenicagar
100.072000 to72000
0.004.862.823COLI ID PP 37-44
100.049000 to49000
0.004.690.9301VRBA PP 37-44
91.91500 to104000
0.184.8235.51138Petrifilm
85.746000 to110000
0.055.006.5417MPN
92.98200 to120000
0.164.8013.08014Other
94.21500 to121000
0.164.821005107All
Comments
Page 54 of 123Issue: 1 09 October 2018
Sample: 16D - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
Escherichia coli is found in human and animal intestines, and, therefore, the presence of the organism in foods is thought to indicate faecalcontamination. It is historically used as an 'indicator' organism, to indicate whether more serious pathogens, such as Salmonella are alsolikely to be present.
Page 55 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.53215,333PCA PP 30MC0053
0.40194,000PetrifilmMC0054
-0.30110,000PCA PP 30MC0067
0.09150,000PetrifilmMC0067
-0.09130,000PCA PP 30MC0143
-1.1754,600OtherMC0151
0.20165,000PCA PP 30MC0219
-0.8371,971PCA SP 30MC0247
0.00140,000OtherMC0282
-0.42100,000PetrifilmMC0307
0.10152,000PCA PP 30MC0379
0.00140,000OtherMC0390
0.44200,000PCA PP 30MC0431
0.44200,000PCA PP 30MC0434
0.38190,000PCA PP 30MC0437
0.44200,000PCA SP 30MC0437
0.50210,000OtherMC0440
0.17160,000PCA PP 30MC0440
0.62230,000OtherMC0483
-0.30110,000PCA PP 30MC0483
0.09150,000PCA PP 30MC0520
0.01141,438PCA PP 30MC0596
0.40194,000PCA PP 30MC0683
-0.05135,000PCA PP 30MC0816
0.77260,000PCA PP 30MC0854
1.21370,000PetrifilmMC0907
0.55218,000PCA PP 30MC0914
-2.9812,700PCA PP 37MC0960
0.16159,000PCA PP 30MC0988
0.17160,000PCA PP 30MC1065
0.38190,000PetrifilmMC1067
-0.35106,000PCA PP 30MC1073
0.17160,000PCA PP 30MC1096
0.00140,000PCA PP 30MC1097
-0.15124,500PCA PP 30MC1121
0.56220,000PCA PP 30MC1127
-0.20119,000PCA PP 30MC1139
-0.28112,000PCA PP 30MC1166
0.15157,500OtherMC1175
0.17160,000PetrifilmMC1175
0.31180,000PCA PP 30MC1226
0.09150,000PCA PP 30MC1296
-0.06133,000PetrifilmMC1449
0.15158,000PCA PP 30MC1449
1.36420,000PCA PP 30MC1450
0.44200,000OtherMC1463
0.17160,000PetrifilmMC1463
0.09150,000PCA PP 30MC1463
-0.30110,000PCA PP 30MC1493
0.62230,000PCA PP 30MC1509
-0.42100,000PCA PP 30MC1601
-0.19120,000PCA PP 30MC1602
-0.5888,000OtherMC1653
-0.8372,000PCA PP 30MC1653
0.09150,000PCA PP 30MC1721
-0.30110,000PCA PP 30MC1727
1.74570,000OtherMC1768
0.01141,000PCA PP 30MC1779
-0.09130,000PCA PP 30MC1780
0.09150,000PCA PP 30MC1781
-0.5193,000PCA PP 30MC1876
Page 56 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.03143,000OtherMC1895
-0.05135,000PCA PP 30MC1895
0.09150,000PCA PP 30MC1920
-0.19120,000PCA SP 30MC1946
0.17160,000OtherMC2108
-0.09130,000PetrifilmMC2109
0.08149,000PCA SP 30MC2127
-0.6384,000PCA SP 30MC2178
-0.09130,000PCA PP 30MC2214
0.17160,000PCA SP 30MC2220
0.72250,000PCA PP 30MC2240
0.21165,909PCA PP 30MC2319
0.36187,200PetrifilmMC2357
-0.30110,000PCA SP 30MC2396
-0.4399,000OtherMC2459
-0.07132,000PCA PP 30MC2481
0.38190,000PCA PP 30MC2492
0.38190,000PetrifilmMC2492
0.37188,125PetrifilmMC2510
-0.6980,000PCA PP 30MC2522
-0.17122,000PCA PP 30MC2647
-0.19120,000PCA PP 30MC2798
0.24170,000PCA PP 30MC2813
0.17160,000PCA PP 30MC2850
-0.4895,000PCA PP 30MC2851
0.09151,000PCA PP 30MC2853
-0.21118,000PCA PP 30MC3026
-0.09130,000PCA PP 30MC3102
0.38190,000PCA SP 30MC3109
-0.5888,000PCA PP 37MC3128
0.17160,000PCA PP 30MC3168
1.36420,000PCA PP 30MC3228
1.36420,000PCA SP 30MC3228
-0.42100,000OtherMC3242
0.31180,000PetrifilmMC3243
0.17160,000PetrifilmMC3244
0.17160,000PetrifilmMC3245
-0.9167,000PetrifilmMC3246
-0.42100,000PetrifilmMC3248
0.09150,000PetrifilmMC3249
0.00140,000PetrifilmMC3250
-0.42100,000PetrifilmMC3251
-0.7378,000PetrifilmMC3252
0.09150,000PetrifilmMC3253
-0.4994,000PCA PP 37MC3254
-0.4994,000OtherMC3254
-0.6682,000PCA PP 30MC3255
0.09150,000PetrifilmMC3256
-0.09130,000PCA PP 30MC3256
-0.42100,000PCA PP 30MC3392
-0.30110,000PCA SP 30MC3415
-0.09130,000PCA PP 30MC3493
0.44200,000PetrifilmMC3611
0.27174,000PCA PP 30MC3731
-0.09130,000PCA PP 30MC3744
0.09150,000PetrifilmMC3745
-0.09130,000PetrifilmMC3746
0.86280,000PCA PP 30MC3750
0.62230,000PCA PP 30MC3751
0.00140,000PCA PP 30MC3906
0.95300,000PCA PP 30MC3929
Page 57 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-3.707,100PCA PP 30MC3981
0.31180,000PetrifilmMC3986
0.62230,000PCA PP 30MC3990
0.44200,000PCA SP 30MC3990
1.21370,000OtherMC4005
-0.5193,000PCA PP 30MC4005
0.17160,000PCA PP 30MC4174
0.00140,000PetrifilmMC4262
0.09150,000PCA PP 30MC4364
0.56220,000PCA PP 30MC4369
-0.4796,000PetrifilmMC4371
0.72250,000PetrifilmMC4372
0.17160,000PCA PP 30MC4385
0.03144,000PCA PP 30MC4566
0.32181,818PCA PP 30MC4630
-0.22117,500PCA PP 30MC4637
-0.30110,000PCA PP 30MC4666
-0.19120,000PCA PP 30MC4672
0.09150,000PCA PP 30MC4688
-0.4895,000PCA PP 30MC4710
-0.30110,227PCA PP 30MC4732
0.24170,000PCA PP 30MC4739
-0.30110,000PCA PP 30MC4752
-0.19120,000PCA PP 30MC4851
-0.42100,000PCA PP 30MC4870
0.37188,181PCA PP 30MC4931
0.37188,636PCA SP 30MC4931
-0.09130,000PCA PP 30MC5009
-0.09130,000PCA PP 37MC5026
-0.9068,000PCA PP 30MC5027
-0.09130,000PCA PP 30MC5094
0.15157,500OtherMC5127
0.16159,000PetrifilmMC5127
-0.11128,000PCA PP 30MC5563
-0.14125,000PetrifilmMC5563
0.09150,000PetrifilmMC5626
0.38190,000PCA PP 30MC5676
1.12345,000PCA PP 37MC5683
-0.23116,000PCA PP 30MC5954
-0.24115,000PCA PP 37MC5954
0.09150,000OtherMC5969
0.44200,000MPCA PP 30MC5970
-0.30110,000OtherMC5989
0.31180,000OtherMC6008
0.56220,000PCA PP 37MC6008
0.00140,000OtherMC6129
0.38190,000PCA SP 30MC6163
-0.19120,000PCA PP 30MC6258
0.56220,000PCA PP 30MC6268
0.31180,000PCA PP 30MC6295
0.15158,000PetrifilmMC6354
-1.0560,000OtherMC6380
-0.19120,000PCA PP 30MC6397
-0.09130,000OtherMC6397
-0.5987,000PCA PP 37MC6397
-0.9465,670OtherMC6400
-0.30110,000PCA PP 30MC6421
-0.4895,000PCA PP 30MC6446
-0.7874,400PCA PP 30MC6447
-0.19120,000PCA PP 30MC6476
-0.30110,000PetrifilmMC6524
Page 58 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.03144,000PetrifilmMC6645
-0.09130,000PetrifilmMC6669
-0.8372,000PetrifilmMC6679
-2.1724,375PCA PP 30MC6707
-1.2850,000OtherMC6750
0.00140,000PetrifilmMC6798
-0.05134,100PCA PP 37MC6838
-0.06133,400PCA SP 30MC6838
-0.07132,600OtherMC6838
-0.09130,000PCA PP 30MC6903
-0.09130,000PCA PP 30MC7099
0.44200,000PCA PP 30MC7112
-0.19120,000PCA PP 30MC7189
-0.5987,000PetrifilmMC7259
-0.19120,000PCA PP 30MC7288
0.09150,000OtherMC7288
-0.09130,000PCA PP 37MC7288
-0.30110,000PCA PP 30MC7289
-0.30110,000PCA PP 37MC7289
-0.5987,000PetrifilmMC7419
0.31180,000PCA PP 30MC7447
0.07148,000PCA PP 30MC7490
0.31180,000PetrifilmMC7496
0.31180,000PetrifilmMC7499
0.09150,000PCA PP 30MC7583
-0.19120,000OtherMC7693
0.72250,000PCA PP 30MC7729
-0.6285,000PCA PP 30MC7775
-0.19120,000PCA PP 30MC7781
0.95300,000PCA PP 30MC7783
0.12154,000OtherMC7869
0.44200,000OtherMC7933
-0.09130,000PCA PP 30MC7937
-0.36105,000PCA PP 37MC7955
-0.42100,000PetrifilmMC7992
-1.0560,000PetrifilmMC8152
-1.7933,200PCA PP 30MC8312
-0.5292,000PCA PP 30MC8949
-0.5391,000PCA SP 30MC8949
2.15790,000OtherMC9510
0.09150,000OtherMC9511
-0.4597,100PCA PP 30MC9521
-0.19120,000PetrifilmMC9526
0.67240,000OtherMC9544
0.04145,000PetrifilmMC9550
-0.39102,500OtherMC9556
-3.548,100PCA PP 37MC9557
0.24170,000PetrifilmMC9571
Value
Data Statistics
231Number of Results
0Number of Excluded Results
5.13 log10Mean
5.15 log10Median
0.23 log10Standard Deviation
0.16 log10Robust Standard Deviation
7100 to 790000 cfu/gResult Range
Page 59 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Total aerobic mesophilic count
Round: 269Scheme: Food Microbiology (QMS)
Performance Statistics
Value
140,000 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
27,934 to 701,662 cfu/gSatisfactory Range
97.8%Satisfactory z scores
1.3%Questionable z scores
0.9%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
98.37100 to420000
0.135.1352.380121PCA PP 30
100.071971 to420000
0.195.176.49015PCA SP 30
84.68100 to345000
0.135.045.63013PCA PP 37
100.0200000 to200000
0.005.300.4301MPCA PP 30
100.060000 to370000
0.125.1820.78048Petrifilm
97.050000 to790000
0.215.1614.29033Other
97.87100 to790000
0.165.151000231All
Comments
The sample contained Bacillus subtilis and Escherichia coli at an assigned value of 140,000 cfu/g.
Total aerobic mesophilic counts are used to determine the general microbial population of a sample.
Page 60 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.04100,000PetrifilmMC0067
0.0097,000VRBA PP 30MC0067
-0.0593,000VRBA PP 37MC0143
0.26120,000VRBA PP 37MC0219
-0.0395,000Chromogenic agarMC0282
-0.0196,000PetrifilmMC0307
0.40134,000VRBA PP 37MC0379
0.29122,334COLI ID PP 37MC0390
0.36130,000VRBA PP 37MC0431
0.26120,000VRBA PP 30MC0434
0.26120,000OtherMC0437
0.26120,000VRBA PP 30MC0437
0.26120,000Chromogenic agarMC0440
1.12240,000MPNMC0440
0.26120,000OtherMC0440
0.54150,000MPNMC0483
0.62160,000OtherMC0483
-0.1189,000VRBA PP 30MC0483
0.30123,453Chromogenic agarMC0596
0.09104,000VRBA PP 37MC0683
0.31125,000COLI ID PP 37MC0816
0.96210,000VRBA PP 37MC0854
0.58155,000VRBA PP 37MC0914
0.32126,000VRBA PP 37MC0988
-0.3275,000OtherMC1065
0.0198,000PetrifilmMC1067
-0.8051,000VRBA PP 30MC1073
0.16110,000VRBA PP 30MC1096
0.04100,000VRBA PP 37MC1097
0.09104,500COLI ID PP 37MC1121
-0.0395,000VRBA PP 37MC1139
-0.1983,000PetrifilmMC1166
0.16110,000VRBA PP 37MC1226
0.16110,000VRBA PP 30MC1296
-0.8250,000PetrifilmMC1449
-0.6060,000VRBA PP 30MC1449
1.44310,000PetrifilmMC1450
-0.0395,000VRBA PP 37MC1493
0.26120,000VRBA PP 30MC1509
-0.3474,000OtherMC1601
-0.4667,000VRBA PP 37MC1601
-0.2977,000VRBA PP 30MC1601
0.16110,000VRBA PP 30MC1602
0.04100,000VRBA PP 37MC1602
-1.0242,600VRBA PP 37MC1653
-0.6060,000VRBA PP 30MC1653
-0.1685,000VRBA PP 30MC1721
-0.4070,000VRBA PP 37MC1727
-0.0990,000OtherMC1768
0.07103,000VRBA PP 37MC1779
-0.4070,000VRBA PP 37MC1780
0.0097,000VRBA PP 37MC1781
-0.3076,000VRBA PP 30MC1876
-0.2480,000OtherMC1895
-0.2977,000VRBA PP 37MC1895
0.46140,000VRBA PP 37MC1920
-0.8549,000VRBA PP 30MC1946
0.16110,000OtherMC2108
-0.0792,000VRBA PP 37MC2109
-0.0593,000VRBA PP 37MC2127
-0.2480,000VRBA PP 37MC2178
Page 61 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.1685,000VRBA PP 30MC2214
0.04100,000VRBA PP 37MC2220
-0.7055,000VRBA PP 30MC2240
0.0197,600Chromogenic agarMC2357
-0.9545,000VRBA PP 37MC2396
0.22116,000VRBA PP 37MC2481
-0.0494,000PetrifilmMC2492
0.04100,000VRBA PP 30MC2492
-0.0890,875PetrifilmMC2510
-0.4468,000VRBA PP 30MC2647
-0.2281,000VRBA PP 30MC2798
0.46140,000VRBA PP 37MC2813
0.26120,000Chromogenic agarMC2850
-0.5861,000VRBA PP 30MC2851
0.05101,000VRBA PP 37MC2853
-0.1884,000VRBA PP 30MC3026
-0.4468,000VRBA PP 37MC3128
0.26120,000VRBA PP 30MC3168
1.07230,000VRBA PP 30MC3228
0.16110,000OtherMC3242
-0.0990,000PetrifilmMC3243
0.16110,000PetrifilmMC3244
0.46140,000PetrifilmMC3245
-0.2281,000PetrifilmMC3246
-0.2778,000PetrifilmMC3248
-0.4468,000PetrifilmMC3249
-0.3573,000PetrifilmMC3250
-0.3772,000PetrifilmMC3251
-0.0990,000PetrifilmMC3252
0.54150,000PetrifilmMC3253
-0.3573,000OtherMC3254
>1,100MPNMC3255
-0.3275,000PetrifilmMC3255
0.16110,000PetrifilmMC3256
-0.6657,000VRBA PP 30MC3256
-0.4070,000VRBA PP 30MC3392
-0.1983,000VRBA PP 30MC3415
0.04100,000VRBA PP 30MC3493
0.46140,000Chromogenic agarMC3611
0.16110,000MPNMC3744
-0.4468,000VRBA PP 30MC3744
0.16110,000PetrifilmMC3744
0.36130,000PetrifilmMC3745
-0.6856,000PetrifilmMC3746
0.46140,000Chromogenic agarMC3751
-0.2281,000VRBA PP 30MC3906
-2.919,300MPNMC3981
0.54150,000PetrifilmMC3986
0.04100,000VRBA PP 37MC3990
-0.8549,000VRBA PP 37MC4005
-0.8549,000OtherMC4005
-0.2480,000PetrifilmMC4174
0.26120,000PetrifilmMC4262
-0.1288,000OtherMC4327
-0.1983,000VRBA PP 37MC4327
-0.0990,000VRBA PP 30MC4364
0.46140,000VRBA PP 37MC4369
-0.2778,000PetrifilmMC4371
1.22260,000PetrifilmMC4372
0.26120,000VRBA PP 37MC4385
-0.2977,000OtherMC4666
Page 62 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.26120,000VRBA PP 37MC4688
-0.5861,000VRBA PP 30MC4710
0.36130,000OtherMC4722
0.20114,000VRBA PP 37MC4739
-0.1288,000PetrifilmMC4752
0.46140,000Chromogenic agarMC4799
-0.6060,000VRBA PP 30MC4851
0.04100,000VRBA PP 37MC5026
-0.6259,000VRBA PP 37MC5027
<10Chromogenic agarMC5094
0.15109,500OtherMC5127
0.18112,000PetrifilmMC5127
0.06102,000PetrifilmMC5563
0.0198,000VRBA PP 37MC5563
0.36130,000VRBA PP 30MC5676
1.12240,000MPNMC5683
0.0399,000VRBA PP 37MC5954
0.26120,000OtherMC5969
0.16110,000OtherMC5989
0.62160,000OtherMC6008
0.90200,000VRBA PP 37MC6008
-0.0196,000PetrifilmMC6129
0.04100,000VRBA PP 37MC6163
0.04100,000VRBA PP 37MC6258
0.54150,000VRBA PP 30MC6268
-0.3772,000VRBA PP 30MC6295
-0.8549,000PetrifilmMC6354
0.46140,000PetrifilmMC6369
0.26120,000OtherMC6369
-0.8250,000PetrifilmMC6380
-0.3474,000VRBA PP 30MC6397
-0.2977,000OtherMC6397
-0.6259,000VRBA PP 37MC6397
-0.9844,000OtherMC6400
0.0097,000VRBA PP 30MC6421
-0.9844,000VRBA PP 37MC6446
-0.3275,000VRBA PP 37MC6447
0.04100,000VRBA PP 37MC6476
0.04100,000PetrifilmMC6524
-0.2579,000PetrifilmMC6669
-0.3573,000PetrifilmMC6679
-2.1217,500VRBA PP 30MC6707
-1.1638,000OtherMC6750
-0.0891,000OtherMC6798
0.37131,000VRBA PP 37MC6838
0.36130,000OtherMC6838
0.35129,000Chromogenic agarMC6838
-0.2182,000VRBA PP 37MC6903
0.04100,000VRBA PP 37MC7099
-0.0395,000VRBA PP 30MC7112
>1,500PetrifilmMC7189
-0.9545,000PetrifilmMC7259
0.16110,000VRBA PP 37MC7288
-0.0196,000VRBA PP 37MC7289
-0.4667,000PetrifilmMC7419
0.04100,000VRBA PP 37MC7447
-0.6259,000VRBA PP 37MC7490
0.04100,000PetrifilmMC7496
0.04100,000PetrifilmMC7499
-0.1884,000VRBA PP 37MC7583
0.16110,000OtherMC7693
Page 63 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.3573,000VRBA PP 37MC7729
0.04100,000Chromogenic agarMC7781
0.26120,000OtherMC7869
0.36130,000OtherMC7933
-0.0990,000PetrifilmMC7937
0.09104,545OtherMC7955
-0.3772,000PetrifilmMC7992
1.07230,000OtherMC7998
-0.8250,000PetrifilmMC8152
-1.7623,400VRBA PP 37MC8312
-0.4468,000OtherMC8949
-0.7055,000VRBA PP 30MC8949
3.011,100,000MPNMC9510
-0.0692,500OtherMC9511
-0.2480,000PetrifilmMC9526
>1,100MPNMC9544
-0.3474,000PetrifilmMC9550
-0.3175,500VRBA PP 37MC9556
-6.00770PetrifilmMC9557
0.46140,000PetrifilmMC9571
Value
Data Statistics
203Number of Results
5Number of Excluded Results
4.97 log10Mean
4.99 log10Median
0.20 log10Standard Deviation
0.14 log10Robust Standard Deviation
9300 to 1100000 cfu/gResult Range
Performance Statistics
Value
97,000 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
19,354 to 486,152 cfu/gSatisfactory Range
98.0%Satisfactory z scores
1.0%Questionable z scores
1.0%Unsatisfactory z scores
Page 64 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Coliforms
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.023400 to210000
0.125.0029.06059VRBA PP 37
97.617500 to230000
0.184.9220.2041VRBA PP 30
100.0104500 to125000
0.015.091.4803COLI ID PP 37
100.095000 to140000
0.095.095.42111Chromogenicagar
66.79300 to1100000
0.255.283.9428MPN
98.045000 to310000
0.134.9524.63250Petrifilm
100.038000 to230000
0.125.0415.27031Other
98.09300 to1100000
0.144.991005203All
Comments
The sample contained Escherichia coli at an assigned value of 97,000 cfu/g.
Coliforms are found in human and animal intestines, and their presence in foods is used as an indicator of faecal contamination. However,many coliforms are also found throughout the environment, being isolated from air, soil, water, plants, and animals. The presence ofcoliform indicator organisms in foods may, therefore, be indicative of faecal contamination, but may also be environmental in origin.
Page 65 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.66170,778VRBGA PP 37MC0053
-0.0993,000VRBGA PP 37MC0143
0.12110,000VRBGA PP 37MC0219
-0.0596,000VRBGA PP 37MC0282
-0.7953,000PetrifilmMC0307
0.00100,000VRBGA PP 37MC0379
0.50150,000VRBGA PP 37MC0431
0.33130,000VRBGA PP 30MC0434
0.23120,000VRBGA PP 37MC0437
0.66170,000OtherMC0440
0.23120,000VRBGA PP 37MC0440
0.37135,000OtherMC0483
-0.0894,000VRBGA PP 37MC0483
-0.2383,000VRBGA PP 37MC0520
0.27123,855VRBGA PP 37MC0596
0.58160,000VRBGA PP 37MC0683
0.54155,000VRBGA PP 37MC0816
0.92210,000VRBGA PP 37MC0854
>30,000VRBGA PP 37MC0902
0.33130,000PetrifilmMC0907
-0.4370,800VRBGA PP 37MC0960
0.29126,000VRBGA PP 37MC0988
0.23120,000VRBGA PP 37MC1065
-0.1787,000PetrifilmMC1067
0.07106,000VRBGA PP 37MC1073
0.33130,000VRBGA PP 37MC1096
-0.3277,000VRBGA PP 37MC1097
0.02102,000VRBGA PP 37MC1121
0.33130,500VRBGA PP 37MC1127
-0.0199,000VRBGA PP 37MC1139
0.34132,000PetrifilmMC1166
0.16114,000OtherMC1175
0.26123,000VRBGA PP 37MC1175
0.23120,000VRBGA PP 37MC1226
-0.0398,000VRBGA PP 37MC1296
-0.5962,000PetrifilmMC1449
-0.4470,000VRBGA PP 37MC1449
1.52340,000PetrifilmMC1450
-0.0695,000VRBGA PP 37MC1493
-0.2582,000VRBGA PP 30MC1509
0.00100,000OtherMC1509
-0.0199,000VRBGA PP 37MC1601
-0.1787,000VRBGA PP 30MC1601
-0.1291,000OtherMC1601
0.00100,000VRBGA PP 30MC1602
-0.0199,000VRBGA PP 37MC1602
-1.2935,500VRBGA PP 37MC1653
-1.7524,400OtherMC1653
0.00100,000VRBGA PP 37MC1721
-0.3675,000VRBGA PP 37MC1727
0.08107,000VRBGA PP 37MC1779
-0.3277,000VRBGA PP 37MC1780
-0.0497,000VRBGA PP 37MC1781
0.12110,000VRBGA PP 37MC1876
-0.1291,000OtherMC1895
-0.1688,000VRBGA PP 37MC1895
0.12110,000VRBGA PP 37MC1920
0.00100,000VRBGA PP 37MC1946
-0.0398,000OtherMC2108
0.00100,000PetrifilmMC2109
0.17115,000VRBGA PP 37MC2127
Page 66 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.1390,000VRBGA PP 37MC2178
0.12110,000VRBGA PP 37MC2214
0.00100,000VRBGA PP 37MC2220
-0.5067,000VRBGA PP 37MC2240
0.47146,000PetrifilmMC2357
-0.8451,000VRBGA PP 37MC2396
-0.5365,000VRBGA PP 37MC2459
0.19117,000VRBGA PP 37MC2481
0.12110,000PetrifilmMC2492
0.33130,000VRBGA PP 37MC2492
-0.6858,000VRBGA PP 37MC2522
-0.1787,000VRBGA PP 37MC2647
-0.3675,000VRBGA PP 30MC2798
0.12110,000VRBGA PP 37MC2813
0.23120,000VRBGA PP 37MC2850
-0.3675,000MPNMC2851
-0.2383,000VRBGA PP 30MC2851
0.22119,000VRBGA PP 37MC2853
-0.0695,000VRBGA PP 30MC3026
-0.1986,000VRBGA PP 37MC3102
0.15113,000VRBGA PP 37MC3109
-0.4470,000VRBGA PP 37MC3128
0.42140,000VRBGA PP 37MC3168
1.09240,000VRBGA PP 37MC3228
-0.4470,000PetrifilmMC3242
-0.2085,000PetrifilmMC3243
0.00100,000PetrifilmMC3244
0.42140,000PetrifilmMC3245
0.00100,000PetrifilmMC3246
-0.0695,000PetrifilmMC3248
0.00100,000PetrifilmMC3249
-0.2979,000PetrifilmMC3250
-0.7455,000PetrifilmMC3251
-0.5266,000VRBGA PP 37MC3252
0.05104,000PetrifilmMC3253
-0.2284,000PetrifilmMC3254
-0.2284,000VRBGA PP 37MC3255
-0.2979,000VRBGA PP 37MC3256
-0.2880,000VRBGA PP 37MC3392
0.42140,000VRBGA PP 37MC3415
0.00100,000VRBGA PP 37MC3493
-0.2284,000VRBGA PP 37MC3611
0.55156,000VRBGA PP 37MC3731
-0.0894,000VRBGA PP 37MC3744
0.00100,000PetrifilmMC3745
-0.5763,000PetrifilmMC3746
1.44320,000VRBGA PP 30MC3750
0.42140,000OtherMC3751
-0.1390,000VRBGA PP 37MC3906
1.36300,000VRBGA PP 30MC3929
-3.476,100VRBGA PP 37MC3981
0.42140,000PetrifilmMC3986
0.12110,000VRBGA PP 37MC3990
-0.8949,000OtherMC4005
-0.8949,000VRBGA PP 37MC4005
-1.4531,000PetrifilmMC4174
0.23120,000PetrifilmMC4262
-0.1390,000VRBGA PP 37MC4364
0.58160,000VRBGA PP 37MC4369
-0.4172,000PetrifilmMC4371
0.92210,000PetrifilmMC4372
Page 67 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.42140,000VRBGA PP 37MC4385
-0.1191,375VRBGA PP 37MC4637
-0.1688,000VRBGA PP 37MC4666
-0.4868,000VRBGA PP 37MC4672
0.33130,000VRBGA PP 37MC4688
-0.3675,000MPNMC4710
-0.2383,000VRBGA PP 30MC4710
0.23120,000PetrifilmMC4722
-0.0397,500VRBGA PP 37MC4732
0.33130,000VRBGA PP 37MC4739
0.12110,000VRBGA PP 37MC4752
0.33130,000VRBGA PP 30MC4797
0.33130,000VRBGA PP 37MC4799
-0.7654,000VRBGA PP 37MC4851
-0.2979,000VRBGA PP 37MC4870
0.33130,909VRBGA PP 37MC4931
0.12110,000VRBGA PP 37MC5009
0.12110,000VRBGA PP 37MC5026
-0.5763,000VRBGA PP 37MC5027
0.04103,640VRBGA PP 37MC5094
-0.2681,000PetrifilmMC5563
-0.2880,000VRBGA PP 37MC5563
0.42140,000VRBGA PP 37MC5676
0.95215,000PetrifilmMC5683
0.08106,670VRBGA PP 37MC5954
0.23120,000VRBGA PP 37MC5970
0.23120,000VRBGA PP 37MC5989
0.86200,000PetrifilmMC6008
0.92210,000VRBGA PP 30MC6008
0.66170,000OtherMC6008
0.12110,000VRBGA PP 37MC6163
0.00100,000VRBGA PP 37MC6258
0.50150,000VRBGA PP 37MC6268
-0.1092,000VRBGA PP 37MC6295
-0.6858,000PetrifilmMC6380
-0.0497,000VRBGA PP 30MC6397
-0.1291,000OtherMC6397
-0.0497,000VRBGA PP 37MC6397
-1.0044,670PetrifilmMC6400
0.00100,000VRBGA PP 37MC6421
-0.6161,000VRBGA PP 37MC6446
-0.9347,200OtherMC6447
0.12110,000VRBGA PP 37MC6476
-0.0398,000PetrifilmMC6645
-0.1787,000PetrifilmMC6669
-0.4271,000PetrifilmMC6679
-3.307,000OtherMC6750
0.23120,000OtherMC6798
0.35133,000VRBGA PP 37MC6838
0.33130,200VRBGA PP 30MC6838
0.34131,000OtherMC6838
0.23120,000VRBGA PP 37MC6903
0.00100,000VRBGA PP 37MC7099
0.23120,000VRBGA PP 37MC7112
-3.416,400VRBGA PP 37MC7189
-1.0543,000VRBGA PP 37MC7259
0.12110,000VRBGA PP 37MC7288
-0.0894,000VRBGA PP 37MC7289
-0.5763,000PetrifilmMC7419
-0.2681,000VRBGA PP 37MC7490
-0.1092,000VRBGA PP 37MC7583
Page 68 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.3675,000VRBGA PP 37MC7729
-0.1688,000VRBGA PP 37MC7775
0.42140,000VRBGA PP 37MC7783
0.00100,000VRBGA PP 37MC7937
-0.2482,272VRBGA PP 37MC7955
-0.2880,000PetrifilmMC7992
-0.8451,000PetrifilmMC8152
-1.7724,000VRBGA PP 37MC8312
-0.1986,000OtherMC8949
-0.2383,000VRBGA PP 37MC8949
-0.1092,500OtherMC9511
-0.0893,600VRBGA PP 37MC9521
-0.2383,000PetrifilmMC9526
0.00100,000PetrifilmMC9550
0.05104,000VRBGA PP 37MC9556
Value
Data Statistics
198Number of Results
1Number of Excluded Results
4.97 log10Mean
5.00 log10Median
0.22 log10Standard Deviation
0.12 log10Robust Standard Deviation
6100 to 340000 cfu/gResult Range
Performance Statistics
Value
100,000 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
19,953 to 501,187 cfu/gSatisfactory Range
98.5%Satisfactory z scores
0.0%Questionable z scores
1.5%Unsatisfactory z scores
Page 69 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Enterobacteriaceae
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
98.46100 to240000
0.125.0062.121123VRBGA PP 37
100.075000 to320000
0.154.997.07014VRBGA PP 30
100.031000 to340000
0.184.9820.71041Petrifilm
100.075000 to75000
0.004.881.0102MPN
94.47000 to170000
0.184.989.09018Other
98.56100 to340000
0.125.001001198All
Comments
The sample contained Escherichia coli at an assigned value of 100,000 cfu/g.
Members of the Enterobacteriaceae family are distributed worldwide and found in soil, water, fruits, vegetables, grains, flowering plants, anda wide range of animals, from insects to humans.
Page 70 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.18112,000PetrifilmMC0054
0.04100,000PetrifilmMC0067
0.04100,000TBX PP 37-44MC0067
-0.0593,000TBX PP 37-44MC0143
0.27120,000COLI ID PP 37-44MC0219
-0.0295,000Chromogenic agarMC0282
-0.0196,000PetrifilmMC0307
-0.2380,000PetrifilmMC0379
0.29122,334COLI ID PP 37-44MC0390
0.16110,000TBX PP 37-44MC0431
0.37130,000TBX PP 37-44MC0434
0.27120,000TBX PP 37-44MC0437
0.27120,000MPNMC0440
0.96210,000OtherMC0440
0.27120,000TBX PP 37-44MC0440
0.55150,000MPNMC0483
0.51145,000OtherMC0483
0.22115,000TBX PP 37-44MC0483
-0.0196,000TBX PP 37-44MC0520
0.31123,453Chromogenic agarMC0596
-0.0692,000TBX PP 37-44MC0683
0.67165,000COLI ID PP 37-44MC0816
0.90200,000TBX PP 37-44MC0854
>30,000TBX PP 37-44MC0902
-0.3076,000TBX PP 37-44MC0988
-0.0394,000OtherMC1065
0.04100,000PetrifilmMC1067
-0.4965,000TBX PP 37-44MC1073
0.16110,000TBX PP 37-44MC1096
0.27120,000TBX PP 37-44MC1097
0.10104,500COLI ID PP 37-44MC1121
0.44138,000Chromogenic agarMC1127
-0.7752,000OtherMC1127
-0.4070,000OtherMC1139
-0.1983,000PetrifilmMC1166
0.12106,000Chromogenic agarMC1175
0.04100,000TBX PP 37-44MC1226
0.16110,000OtherMC1296
-1.0342,000Chromogenic agarMC1449
-0.6557,000TBX PP 37-44MC1449
1.45310,000PetrifilmMC1450
-0.5562,000TBX PP 37-44MC1493
0.27120,000TBX PP 37-44MC1509
-0.2877,000TBX PP 37-44MC1601
-0.3175,000OtherMC1601
-0.0295,000TBX PP 37-44MC1602
-0.3871,000OtherMC1653
-0.4766,000PetrifilmMC1653
-0.3374,000TBX PP 37-44MC1721
-0.4766,000COLI ID PP 37-44MC1727
-0.3175,000OtherMC1768
-0.0196,000TBX PP 37-44MC1779
-0.1784,000TBX PP 37-44MC1780
-0.2380,000MPNMC1781
-0.0791,000TBX PP 37-44MC1876
-1.0043,000OtherMC1895
-0.9246,000TBX PP 37-44MC1895
0.04100,000COLI ID PP 37-44MC1920
0.27120,000PetrifilmMC1946
0.04100,000OtherMC2108
0.04100,000PetrifilmMC2109
Page 71 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.24117,000TBX PP 37-44MC2127
-0.4766,000OtherMC2178
-0.3175,000TBX PP 37-44MC2214
0.04100,000TBX PP 37-44MC2220
-0.4368,000TBX PP 37-44MC2240
0.0197,600Chromogenic agarMC2357
-0.7453,000TBX PP 37-44MC2396
0.15109,000TBX PP 37-44MC2481
0.37130,000PetrifilmMC2492
0.37130,000TBX PP 37-44MC2492
-0.0791,250PetrifilmMC2510
-0.5164,000TBX PP 37-44MC2647
-0.3374,000COLI ID PP 37-44MC2798
0.27120,000COLI ID PP 37-44MC2813
0.27120,000TBX PP 37-44MC2850
-0.2579,000MPNMC2851
-0.3573,000TBX PP 37-44MC2851
0.0499,500Chromogenic agarMC2853
-0.1983,000TBX PP 37-44MC3102
0.29122,000TBX PP 37-44MC3109
-0.8748,000VRBA PP 37-44MC3128
-0.1685,000TBX PP 37-44MC3168
1.56340,000TBX PP 37-44MC3228
0.16110,000OtherMC3242
-0.0990,000PetrifilmMC3243
0.16110,000PetrifilmMC3244
0.37130,000PetrifilmMC3245
-0.2281,000PetrifilmMC3246
-0.2678,000PetrifilmMC3248
-0.4368,000PetrifilmMC3249
-0.4965,000PetrifilmMC3250
-0.2579,000PetrifilmMC3251
-0.0990,000PetrifilmMC3252
0.37130,000PetrifilmMC3253
-0.2579,000OtherMC3254
-0.3175,000PetrifilmMC3255
>1,100MPNMC3255
>11,000MPNMC3256
0.16110,000PetrifilmMC3256
-0.4070,000TBX PP 37-44MC3392
-0.7055,000TBX PP 37-44MC3415
-0.1685,000COLI ID PP 37-44MC3493
0.46140,000Chromogenic agarMC3611
0.30123,000PetrifilmMC3731
0.16110,000MPNMC3744
0.16110,000PetrifilmMC3744
0.27120,000PetrifilmMC3745
-0.6856,000PetrifilmMC3746
0.55150,000Chromogenic agarMC3751
0.04100,000TBX PP 37-44MC3906
1.41300,000TBX PP 37-44MC3929
-2.6911,000MPNMC3981
0.55150,000PetrifilmMC3986
0.0298,000TBX PP 37-44MC3990
-1.1937,000OtherMC4005
-5.551,100MPNMC4005
1.33281,500TBX PP 37-44MC4005
0.27120,000TBX PP 37-44MC4174
0.37130,000PetrifilmMC4174
0.27120,000PetrifilmMC4262
-0.3672,000TBX PP 37-44MC4364
Page 72 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.37130,000TBX PP 37-44MC4369
-0.2678,000PetrifilmMC4371
1.18250,000PetrifilmMC4372
0.16110,000TBX PP 37-44MC4385
-0.1287,750PetrifilmMC4637
-0.5164,000TBX PP 37-44MC4666
0.16110,000TBX PP 37-44MC4688
-0.2579,000MPNMC4710
-0.3573,000TBX PP 37-44MC4710
0.27120,000PetrifilmMC4722
0.27120,000TBX PP 37-44MC4739
-0.1188,000PetrifilmMC4752
0.46140,000Chromogenic agarMC4799
-0.1685,000TBX PP 37-44MC4851
-0.2281,000TBX PP 37-44MC4870
0.54149,545TBX PP 37-44MC4931
0.0298,000TBX PP 37-44MC5009
0.04100,000VRBA PP 37-44MC5026
-1.0342,000TBX PP 37-44MC5027
0.27120,000Chromogenic agarMC5094
0.18112,000PetrifilmMC5127
-1.0940,000PetrifilmMC5563
-0.9744,000TBX PP 37-44MC5563
-0.0990,000PetrifilmMC5626
0.37130,000TBX PP 37-44MC5676
1.13240,000MPNMC5683
-5.551,100MPNMC5969
0.16110,000MPNMC5989
0.96210,000PetrifilmMC6008
0.63160,000OtherMC6008
0.70170,000TBX PP 37-44MC6008
-0.0196,000PetrifilmMC6129
-0.1685,000TBX PP 37-44MC6163
-0.1486,000TBX PP 37-44MC6258
0.55150,000TBX PP 37-44MC6268
-0.2678,000TBX PP 37-44MC6295
-0.0295,000PetrifilmMC6354
0.46140,000PetrifilmMC6369
0.27120,000OtherMC6369
-2.8110,000TBX PP 37-44MC6380
-1.4929,000PetrifilmMC6397
-1.4530,000OtherMC6397
-0.9545,000TBX PP 37-44MC6397
-0.0593,000TBX PP 37-44MC6421
-1.7423,800TBX PP 37-44MC6446
-0.9246,000TBX PP 37-44MC6447
0.37130,000TBX PP 37-44MC6476
0.04100,000PetrifilmMC6524
-0.3374,000PetrifilmMC6645
-0.2579,000PetrifilmMC6669
-0.3573,000PetrifilmMC6679
-1.0940,000OtherMC6750
0.27120,000PetrifilmMC6798
0.38131,000VRBA PP 37-44MC6838
0.37130,000OtherMC6838
0.36129,000Chromogenic agarMC6838
0.0298,000TBX PP 37-44MC6903
-0.0990,000TBX PP 37-44MC7099
0.27120,000TBX PP 37-44MC7112
>1,500PetrifilmMC7189
-0.9545,000PetrifilmMC7259
Page 73 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.1486,000TBX PP 37-44MC7288
0.04100,000OtherMC7288
-0.1486,000TBX PP 37-44MC7289
-0.1486,000OtherMC7289
-0.4567,000PetrifilmMC7419
0.04100,000TBX PP 37-44MC7447
-0.5562,000TBX PP 37-44MC7490
-0.2281,000TBX PP 37-44MC7583
-0.2678,000OtherMC7693
0.0197,000TBX PP 37-44MC7729
0.04100,000Chromogenic agarMC7781
0.41134,000PetrifilmMC7783
1.13240,000TBX PP 37-44MC7783
0.04100,000OtherMC7869
0.34127,000OtherMC7933
-0.7055,000TBX PP 37-44MC7937
0.16110,000MPNMC7955
-0.3672,000PetrifilmMC7992
1.08230,000OtherMC7998
-1.1638,000PetrifilmMC8152
-1.8322,000TBX PP 37-44MC8312
-0.2579,000TBX PP 37-44MC8949
1.94460,000MPNMC9510
-0.1784,000PetrifilmMC9511
-0.5761,000PetrifilmMC9526
-8.8875MPNMC9544
-0.6258,500TBX PP 37-44MC9556
<10PetrifilmMC9557
0.46140,000PetrifilmMC9571
Value
Data Statistics
212Number of Results
8Number of Excluded Results
4.97 log10Mean
4.98 log10Median
0.22 log10Standard Deviation
0.14 log10Robust Standard Deviation
10000 to 460000 cfu/gResult Range
Performance Statistics
Value
96,499 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
19,254 to 483,641 cfu/gSatisfactory Range
97.6%Satisfactory z scores
1.0%Questionable z scores
1.4%Unsatisfactory z scores
Page 74 of 123Issue: 1 09 October 2018
Sample: 16F - Indicator Combination
Analyte: Escherichia coli
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
98.810000 to340000
0.164.9740.57186TBX PP 37-44
100.042000 to150000
0.105.086.13013Chromogenicagar
100.066000 to165000
0.105.024.2509COLI ID PP 37-44
100.048000 to131000
0.175.001.4203VRBA PP 37-44
100.029000 to310000
0.154.9827.83259Petrifilm
71.411000 to460000
0.215.047.55516MPN
100.030000 to230000
0.174.9512.26026Other
97.610000 to460000
0.144.981008212All
Comments
The sample contained Escherichia coli at an assigned value of 96,499 cfu/g.
Escherichia coli is found in human and animal intestines, and, therefore, the presence of the organism in foods is thought to indicate faecalcontamination. It is historically used as an 'indicator' organism, to indicate whether more serious pathogens, such as Salmonella are alsolikely to be present.
Page 75 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Staphylococcus species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.113,100PetrifilmMC1865
0.204,000BP SP 37MC2264
0.806,500BP SP 37MC2728
0.736,100BP SP 37MC4670
0.294,300BP + RPF SP 37MC4975
0.003,400BP SP 37MC5861
-0.163,000OtherMC5861
0.354,500BP SP 37MC6157
-0.382,500OtherMC6397
-0.662,000BP + RPF PP 37MC6397
-0.163,000BP SP 37MC6397
0.786,400BP SP 37MC7825
-0.602,100OtherMC8169
-1.191,300BP SP 37MC8255
0.454,900BP + RPF PP 37MC8395
0.404,700PetrifilmMC9347
-0.851,710PetrifilmMC9581
Value
Data Statistics
17Number of Results
0Number of Excluded Results
3.53 log10Mean
3.53 log10Median
0.21 log10Standard Deviation
0.21 log10Robust Standard Deviation
1300 to 6500 cfu/gResult Range
Performance Statistics
Value
3,400 cfu/gAssigned Value
0.06 log10Uncertainty of Assigned Value
0.35 log10SDPA
678 to 17,040 cfu/gSatisfactory Range
100.0%Satisfactory z scores
0.0%Questionable z scores
0.0%Unsatisfactory z scores
Page 76 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Staphylococcus species
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.01300 to 65000.233.6347.0608BP SP 37
100.04300 to 43000.003.635.8801BP + RPF SP 37
100.02000 to 49000.293.5011.7602BP + RPF PP 37
100.01710 to 47000.273.4917.6503Petrifilm
100.02100 to 30000.113.4017.6503Other
100.01300 to 65000.213.53100017All
Comments
The sample contained Staphylococcus aureus at an assigned value of 3,400 cfu/g.
Staphylococcus species are mainly associated with the skin and mucous membranes of warm-blooded vertebrates, but are also found infood products, dust and water. Strains of Staphylococci other than Staphylococcus aureus are commonly referred to as coagulase-negativestaphylococci. They are not normally pathogenic, although they can cause wound and urine infections, and are not normally associated withfood spoilage.
Page 77 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.632,205PetrifilmMC0044
0.234,400OtherMC0063
-2.22615BP SP 37MC0079
-0.522,420BP SP 37MC0141
-0.013,640BP SP 37MC0189
-0.622,220BP SP 37MC0192
0.154,140BP SP 37MC0193
0.073,888BP SP 37MC0249
0.083,900BP SP 37MC0261
0.385,000BP SP 37MC0309
0.385,000BP SP 37MC0495
0.043,780BP + RPF SP 37MC0575
0.515,550BP + RPF SP 37MC0644
0.525,600BP + RPF PP 37MC0649
0.043,800BP SP 37MC0729
0.174,200BP SP 37MC0754
-0.083,450BP + RPF PP 37MC1046
-0.432,600BP + RPF PP 37MC1059
-0.292,900PetrifilmMC1130
0.174,200BP SP 37MC1139
-0.023,600BP + RPF PP 37MC1159
-0.632,200OtherMC1165
0.676,300BP + RPF SP 37MC1259
0.083,900PetrifilmMC1259
-0.013,636BP SP 37MC1336
0.043,800BP + RPF PP 37MC1363
-2.52480BP SP 37MC1402
0.254,500BP + RPF SP 37MC1509
0.234,400BP SP 37MC1509
-0.093,400BP SP 37MC1554
0.023,733BP SP 37MC1579
-0.322,840BP SP 37MC1580
0.234,400BP + RPF SP 37MC1630
-0.253,000BP SP 37MC1923
0.043,800BP SP 37MC1950
0.696,400BP SP 37MC1970
-2.81380BP SP 37MC1980
-1.061,560BP + RPF SP 37MC1995
0.485,400BP SP 37MC2061
-2.92350BP SP 37MC2095
-0.552,350BP SP 37MC2127
0.786,900BP + RPF SP 37MC2264
0.114,000BP SP 37MC2264
0.043,800BP SP 37MC2282
-0.382,700BP + RPF PP 37MC2287
-0.292,900BP SP 37MC2287
-1.111,500BP + RPF SP 37MC2316
0.435,200BP + RPF PP 37MC2388
-0.292,900PetrifilmMC2436
-0.552,360BP SP 37MC2481
0.144,100BP SP 37MC2490
-0.692,100BP SP 37MC2577
0.716,500BP SP 37MC2728
0.013,700BP + RPF SP 37MC2824
0.284,600BP + RPF PP 37MC2860
0.575,800BP + RPF SP 37MC2951
0.254,500BP + RPF PP 37MC3072
0.144,100BP SP 37MC3072
0.435,200BP + RPF PP 37MC3076
0.807,000BP SP 37MC3076
-0.702,090BP SP 37MC3119
Page 78 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.093,400BP + RPF SP 37MC3172
0.013,700BP SP 37MC3236
0.364,900BP + RPF PP 37MC3236
0.254,500BP + RPF SP 37MC3236
0.063,864BP SP 37MC3269
-0.063,500BP SP 37MC3306
-0.253,000BP + RPF SP 37MC3307
-0.063,500PetrifilmMC3443
-0.133,300Rapid StaphMC3477
-0.093,400BP + RPF SP 37MC3477
-0.253,000BP SP 37MC3477
-0.412,636PetrifilmMC3532
0.174,200BP SP 37MC3574
0.656,200BP SP 37MC3794
-4.05140BP SP 37MC3861
-0.173,200PetrifilmMC3967
-1.391,200OtherMC4034
-0.632,210PetrifilmMC4077
-0.093,400OtherMC4079
0.455,272BP SP 37MC4424
-0.063,500BP + RPF PP 37MC4439
-0.292,900BP SP 37MC4439
0.254,500BP + RPF SP 37MC4553
-0.253,000BP + RPF SP 37MC4602
0.114,000OtherMC4649
0.254,500BP + RPF SP 37MC4685
0.174,200BP + RPF PP 37MC4720
0.254,500BP SP 37MC4829
-0.253,000OtherMC4861
-0.063,500BP SP 37MC4950
-0.023,600BP + RPF PP 37MC4975
0.204,300BP SP 37MC4975
0.204,300OtherMC4975
0.174,200BP SP 37MC5531
-1.76890BP SP 37MC5657
0.043,800OtherMC5666
-0.342,800BP SP 37MC5669
0.304,680BP + RPF SP 37MC5809
0.425,150BP SP 37MC5815
-0.203,136BP + RPF PP 37MC5824
-0.093,400BP SP 37MC5861
-0.253,000OtherMC5861
-0.502,450BP + RPF SP 37MC5965
-0.253,000OtherMC5979
0.254,500BP SP 37MC6157
-0.213,090OtherMC6298
-0.692,100BP + RPF PP 37MC6298
-0.372,727BP SP 37MC6298
-0.253,000OtherMC6353
-0.482,500OtherMC6397
-0.752,000BP + RPF SP 37MC6397
-0.692,100BP SP 37MC6397
-0.482,500BP + RPF PP 37MC6404
0.013,700BP SP 37MC6406
-0.253,000BP + RPF SP 37MC6461
0.234,400PetrifilmMC6680
-0.272,950BP SP 37MC6745
0.415,091BP + RPF PP 37MC6969
0.525,576BP SP 37MC6969
0.435,200OtherMC7057
0.385,000BP + RPF PP 37MC7057
Page 79 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.897,500BP SP 37MC7057
0.525,600BP + RPF SP 37MC7069
-1.051,570BP SP 37MC7332
0.595,900BP SP 37MC7583
-1.291,300OtherMC7640
-0.113,364BP + RPF SP 37MC7647
0.174,200BP SP 37MC7658
-0.881,800OtherMC7777
-0.063,500PetrifilmMC7777
-0.881,800BP SP 37MC7777
>10OtherMC7799
>10OtherMC7800
>10OtherMC7801
0.696,400BP SP 37MC7825
-1.131,481BP SP 37MC8025
0.083,900OtherMC8063
0.093,950BP + RPF PP 37MC8065
-0.951,700OtherMC8169
0.013,700BP + RPF PP 37MC8228
0.174,200BP SP 37MC8266
0.234,400BP + RPF PP 37MC8395
0.525,600OtherMC9232
-0.113,360PetrifilmMC9272
Value
Data Statistics
145Number of Results
3Number of Excluded Results
3.51 log10Mean
3.56 log10Median
0.25 log10Standard Deviation
0.13 log10Robust Standard Deviation
140 to 7500 cfu/gResult Range
Performance Statistics
Value
3,670 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
732 to 18,394 cfu/gSatisfactory Range
96.5%Satisfactory z scores
2.8%Questionable z scores
0.7%Unsatisfactory z scores
Page 80 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
92.4140 to 75000.173.5845.52066BP SP 37
100.01500 to 69000.153.5815.86023BP + RPF SP 37
100.02100 to 56000.123.5915.17022BP + RPF PP 37
100.03300 to 33000.003.520.6901Rapid Staph
100.02205 to 44000.063.517.59011Petrifilm
100.01200 to 56000.193.4815.17322Other
96.5140 to 75000.133.561003145All
Comments
The sample contained Staphylococcus aureus at an assigned value of 3,670 cfu/g.
The most significant food borne coagulase positive staphylococcus is Staphylococcus aureus. Staphylococcus aureus can be present onthe skin, and in the nose and throat of healthy individuals with no ill effect. It is important to test for it in foods as it can be an indicator ofgeneral hygiene and, and be a causative agent of food poisoning.
Page 81 of 123Issue: 1 09 October 2018
The sample contained Bacillus cereus at an assigned value of 6,100 cfu/g.
Sample: 17D - Staph/Bacillus
Analyte: Bacillus species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.7311,000MYP SP 30MC0051
0.197,100MYP SP 30MC1259
0.086,500MYP SP 30MC2264
0.277,600MYP SP 30MC2728
-0.434,300OtherMC4975
-0.274,900MYP SP 30MC5861
-0.155,400OtherMC5861
0.006,100MYP SP 30MC6157
-0.205,200OtherMC6397
-0.274,900BC SP 30MC6397
0.247,400MYP SP 30MC6397
-0.693,500MYP SP 30MC7825
0.489,000MYP SP 30MC8395
Value
Data Statistics
13Number of Results
0Number of Excluded Results
3.78 log10Mean
3.79 log10Median
0.14 log10Standard Deviation
0.14 log10Robust Standard Deviation
3500 to 11000 cfu/gResult Range
Performance Statistics
Value
6,100 cfu/gAssigned Value
0.05 log10Uncertainty of Assigned Value
0.35 log10SDPA
1,217 to 30,572 cfu/gSatisfactory Range
100.0%Satisfactory z scores
0.0%Questionable z scores
0.0%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.03500 to11000
0.103.8569.2309MYP SP 30
100.04900 to 49000.003.697.6901BC SP 30
100.04300 to 54000.023.7223.0803Other
100.03500 to11000
0.143.79100013All
Comments
Page 82 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Bacillus species
Round: 269Scheme: Food Microbiology (QMS)
Bacillus is a large genus of Gram-positive bacilli that are aerobic, facultatively anaerobic and catalase positive. Bacillus species arewidespread in the environment, and are able to survive in adverse conditions due to the formation of endospores. They are able to survivein heat-processed and dried foods. Many species are normally present in soil and decaying animal and vegetable matter. Cells of bacilli arelarge and commonly adhere in chains.
Page 83 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.603,580MYP SP 30MC0044
<20MYP SP 30MC0141
0.046,000OtherMC0309
0.237,000OtherMC0495
-3.76280MYP SP 30MC0575
0.9012,000OtherMC0644
-0.145,200BC SP 30MC0649
0.237,000MYP SP 30MC0676
-0.264,700BC SP 30MC0729
-0.513,850PEMBA SP 30MC0729
-0.264,700MYP SP 30MC1046
-0.374,300MYP SP 30MC1059
-0.125,275BC SP 30MC1093
-0.264,700MYP SP 30MC1139
-0.075,500MYP SP 30MC1159
-0.464,000BC SP 30MC1165
0.206,800MYP SP 30MC1191
0.257,100MYP SP 30MC1259
-0.015,773OtherMC1336
0.126,400MYP SP 30MC1363
-2.95540PEMBA SP 30MC1402
-0.563,700MYP SP 30MC1509
0.066,100MYP SP 30MC1554
-0.683,366MYP SP 30MC1579
0.176,630MYP SP 30MC1580
0.448,300OtherMC1630
-2.061,100PEMBA SP 30MC1865
-0.464,000MYP SP 30MC1923
0.277,200MYP SP 30MC1950
0.7911,000MYP SP 30MC1970
-0.015,750OtherMC1980
-0.075,500MYP SP 30MC2061
-1.381,900MYP SP 30MC2127
0.146,500MYP SP 30MC2264
0.146,500BC SP 30MC2282
-0.075,500MYP SP 30MC2287
0.046,000MYP SP 30MC2316
-0.185,000MYP SP 30MC2388
<1MYP SP 30MC2413
-0.464,000BC SP 30MC2481
0.387,900MYP SP 30MC2490
-0.264,700OtherMC2577
0.035,950OtherMC2605
0.347,600MYP SP 30MC2728
0.066,100Compass BC SP 30MC2753
-0.145,200MYP SP 30MC2824
0.005,800PEMBA SP 30MC2824
0.347,600PEMBA SP 30MC2951
-0.823,000MYP SP 30MC3072
0.7810,866MYP SP 30MC3119
0.146,500Compass BC SP 30MC3172
0.508,700PEMBA SP 30MC3236
0.327,500BC SP 30MC3236
0.418,100MYP SP 30MC3236
-0.683,364BC SP 30MC3269
-0.593,600OtherMC3306
0.9012,000BC SP 30MC3307
-0.185,000MYP SP 30MC3443
-0.095,400Compass BC SP 30MC3477
-0.145,200MYP SP 30MC3477
-0.075,500BC SP 30MC3499
Page 84 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.434,100OtherMC3574
0.046,000PEMBA SP 30MC3794
-1.042,500PEMBA SP 30MC3861
-0.234,800MYP SP 30MC4015
-0.862,900Compass BC SP 30MC4034
0.086,200OtherMC4079
-0.374,302MYP SP 30MC4424
0.257,100MYP SP 30MC4439
-0.095,400MYP SP 30MC4553
0.559,000MYP SP 30MC4602
0.619,500Compass BC SP 30MC4649
0.066,100OtherMC4649
0.408,000MYP SP 30MC4670
0.579,200MYP SP 30MC4685
2.9060,000MYP SP 30MC4720
0.025,900MYP SP 30MC4829
-0.095,400MYP SP 30MC4836
0.025,900OtherMC4861
0.015,864MYP SP 30MC4892
-0.464,000MYP SP 30MC4950
-0.374,300MYP SP 30MC4975
0.237,000MYP SP 30MC5055
-0.214,900MYP SP 30MC5531
-0.344,400MYP SP 30MC5657
0.7911,000PEMBA SP 30MC5666
-0.523,800MYP SP 30MC5669
-0.095,400BC SP 30MC5681
0.498,600MYP SP 30MC5809
0.226,900Compass BC SP 30MC5815
0.015,863Compass BC SP 30MC5824
-0.314,500MYP SP 30MC5861
-0.234,800OtherMC5861
-0.743,200MYP SP 30MC5965
-0.344,400OtherMC5979
0.277,200OtherMC6150
0.066,100MYP SP 30MC6157
-0.473,985OtherMC6298
-0.783,100PEMBA SP 30MC6298
-0.284,636MYP SP 30MC6298
0.408,000MYP SP 30MC6353
-0.145,200OtherMC6397
-0.214,900BC SP 30MC6397
0.126,400MYP SP 30MC6397
-0.902,800MYP SP 30MC6404
0.619,500MYP SP 30MC6461
-0.952,700MYP SP 30MC6680
-0.434,100MYP SP 30MC6745
0.408,000OtherMC6951
0.418,103MYP SP 30MC6969
0.146,500OtherMC7057
0.046,000BC SP 30MC7057
0.206,800MYP SP 30MC7057
0.418,100MYP SP 30MC7069
-0.204,930OtherMC7296
0.257,100MYP SP 30MC7332
-0.743,200MYP SP 30MC7583
0.136,455Compass BC SP 30MC7647
-1.381,900MYP SP 30MC7777
-0.633,500MYP SP 30MC7825
0.448,300MYP SP 30MC7956
0.408,000OtherMC7957
Page 85 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-2.22972MYP SP 30MC8025
0.206,800OtherMC8063
0.106,300MYP SP 30MC8065
0.166,600OtherMC8228
0.005,800MYP SP 30MC8266
0.468,400MYP SP 30MC8395
0.086,200OtherMC9232
Value
Data Statistics
129Number of Results
2Number of Excluded Results
3.73 log10Mean
3.76 log10Median
0.25 log10Standard Deviation
0.14 log10Robust Standard Deviation
280 to 60000 cfu/gResult Range
Performance Statistics
Value
5,800 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
1,157 to 29,069 cfu/gSatisfactory Range
96.1%Satisfactory z scores
3.1%Questionable z scores
0.8%Unsatisfactory z scores
Page 86 of 123Issue: 1 09 October 2018
Sample: 17D - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
95.8280 to 600000.163.7456.59273MYP SP 30
80.0540 to 110000.353.677.75010PEMBA SP 30
100.02900 to 95000.053.806.208Compass BC SP30
100.03364 to12000
0.083.7210.08013BC SP 30
100.03600 to12000
0.103.7819.38025Other
96.1280 to 600000.143.761002129All
Comments
The sample contained Bacillus cereus at an assigned value of 5,800 cfu/g.
In foodstuffs, Bacillus cereus is both a spoilage organism and a causative agent of food poisoning. The organism is commonly found in arange of foods, including rice, eggs, bread, meat, milk and other dairy products.
Page 87 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Staphylococcus species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.503,000OtherMC1619
0.366,000BP + RPF PP 37MC1675
0.004,500BP SP 37MC5027
-0.632,700PetrifilmMC6669
0.486,600BP SP 37MC6722
-0.034,400BP SP 37MC7289
0.275,600BP SP 37MC8312
Due to the low number of results returned, performance scores are shown for information purposes only
Value
Data Statistics
7Number of Results
0Number of Excluded Results
3.65 log10Mean
3.65 log10Median
0.15 log10Standard Deviation
0.19 log10Robust Standard Deviation
2700 to 6600 cfu/gResult Range
Performance Statistics
Value
4,500 cfu/gAssigned Value
0.09 log10Uncertainty of Assigned Value
0.35 log10SDPA
898 to 22,553 cfu/gSatisfactory Range
100.0%Satisfactory z scores
0.0%Questionable z scores
0.0%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.04400 to 66000.083.7057.1404BP SP 37
100.06000 to 60000.003.7814.2901BP + RPF PP 37
100.02700 to 27000.003.4314.2901Petrifilm
100.03000 to 30000.003.4814.2901Other
100.02700 to 66000.193.6510007All
Comments
The sample contained Staphylococcus aureus at an assigned value of 4,500 cfu/g.
Staphylococcus species are mainly associated with the skin and mucous membranes of warm-blooded vertebrates, but are also found infood products, dust and water. Strains of Staphylococci other than Staphylococcus aureus are commonly referred to as coagulase-negativestaphylococci. They are not normally pathogenic, although they can cause wound and urine infections, and are not normally associated withfood spoilage.
Page 88 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.175,500PetrifilmMC0123
-5.5953BP SP 37MC0136
-2.22800PetrifilmMC0307
0.286,000PetrifilmMC0379
2.7142,500BP SP 37MC0390
0.155,400OtherMC0431
0.265,900BP SP 37MC0434
0.034,900BP + RPF PP 37MC0437
0.346,300BP + RPF PP 37MC0440
0.085,100BP SP 37MC0483
0.055,000BP SP 37MC0854
3.1963,000BP SP 37MC0914
0.265,900BP SP 37MC0960
-0.234,000BP + RPF PP 37MC1065
-0.084,500BP + RPF SP 37MC1096
-0.463,300BP SP 37MC1097
0.487,045BP SP 37MC1105
-0.174,180BP SP 37MC1121
-2.62580BP SP 37MC1149
0.286,000BP SP 37MC1226
0.085,100BP SP 37MC1426
-0.543,100PetrifilmMC1449
-0.433,400BP + RPF PP 37MC1449
0.215,700PetrifilmMC1450
0.306,100BP + RPF PP 37MC1483
0.215,700OtherMC1483
0.175,500BP SP 37MC1493
-1.951,000BP SP 37MC1533
0.286,000OtherMC1538
-0.812,500Rapid StaphMC1538
>110OtherMC1601
-0.084,500BP + RPF PP 37MC1601
-0.144,300BP SP 37MC1601
-0.114,400BP + RPF SP 37MC1602
-0.393,500BP SP 37MC1602
-0.393,500BP SP 37MC1653
-0.263,900OtherMC1653
0.286,000BP + RPF PP 37MC1675
0.346,300BP + RPF PP 37MC1684
0.155,400BP + RPF PP 37MC1721
-0.503,200BP SP 37MC1727
-0.463,300BP + RPF PP 37MC1876
-0.114,400BP SP 37MC1876
0.105,200BP + RPF PP 37MC1891
0.195,600OtherMC1895
0.346,300BP SP 37MC1895
0.527,300BP + RPF SP 37MC1920
0.366,400PetrifilmMC1946
0.859,500OtherMC2108
-1.491,440BP SP 37MC2178
-0.114,400BP + RPF PP 37MC2184
-0.084,500OtherMC2184
-0.104,440BP SP 37MC2199
0.366,400BP + RPF PP 37MC2209
0.004,800BP SP 37MC2214
0.286,000BP + RPF PP 37MC2220
0.105,200BP SP 37MC2220
-0.583,000BP + RPF PP 37MC2240
0.175,500BP + RPF SP 37MC2262
0.195,600BP SP 37MC2279
0.004,800BP SP 37MC2283
Page 89 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.483,251OtherMC2321
-0.044,640PetrifilmMC2357
-0.174,200BP SP 37MC2364
-0.293,800BP + RPF SP 37MC2396
-0.114,400OtherMC2468
0.175,500BP SP 37MC2492
0.004,800PetrifilmMC2492
-0.134,325BP + RPF SP 37MC2510
0.9110,000OtherMC2588
0.165,450BP + RPF PP 37MC2647
-0.263,900BP SP 37MC2798
-1.092,000BP SP 37MC2813
0.346,300BP + RPF PP 37MC2850
-0.114,400BP + RPF SP 37MC2851
-0.583,000BP SP 37MC2868
0.456,900BP SP 37MC3000
0.215,700BP SP 37MC3102
0.386,500Rapid StaphMC3102
0.326,200BP + RPF PP 37MC3102
0.386,500BP + RPF PP 37MC3228
0.597,700BP SP 37MC3228
-0.114,400OtherMC3242
0.155,400BP SP 37MC3243
-0.263,900BP SP 37MC3244
-0.503,200BP SP 37MC3245
-0.054,600BP + RPF PP 37MC3246
-0.293,800OtherMC3248
-0.263,900OtherMC3249
-0.263,900BP SP 37MC3250
-0.503,200BP SP 37MC3251
-0.144,300BP SP 37MC3252
0.859,500OtherMC3253
-0.263,900BP + RPF SP 37MC3254
-0.263,900OtherMC3254
-0.054,600BP SP 37MC3255
-0.463,300PetrifilmMC3255
0.235,800BP SP 37MC3256
0.105,200BP + RPF SP 37MC3392
-0.084,500BP SP 37MC3415
-0.174,190Rapid StaphMC3462
0.034,900BP + RPF PP 37MC3465
-0.234,000BP + RPF SP 37MC3471
-0.054,600BP + RPF SP 37MC3493
-0.234,000BP SP 37MC3493
-0.972,200BP SP 37MC3591
0.155,400BP SP 37MC3744
-0.034,700PetrifilmMC3744
-0.712,700PetrifilmMC3745
0.436,800OtherMC3746
-0.234,000BP + RPF PP 37MC3821
0.235,764BP + RPF SP 37MC3824
0.416,700BP + RPF PP 37MC3929
0.195,600BP + RPF PP 37MC3973
0.105,200BP + RPF PP 37MC3990
0.235,800BP SP 37MC3990
0.607,800OtherMC4079
-0.263,900BP + RPF PP 37MC4174
-0.433,400PetrifilmMC4174
-0.393,500PetrifilmMC4193
0.326,200OtherMC4262
0.879,700BP SP 37MC4296
Page 90 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.055,000BP + RPF SP 37MC4339
0.175,500BP + RPF PP 37MC4364
-0.174,200BP + RPF PP 37MC4369
-0.293,800OtherMC4371
-0.234,000PetrifilmMC4372
-0.393,500BP + RPF PP 37MC4385
-0.234,000BP + RPF SP 37MC4584
-0.263,900OtherMC4584
-0.822,475PetrifilmMC4637
0.004,800BP + RPF PP 37MC4688
-0.114,400BP + RPF PP 37MC4710
-0.144,300BP SP 37MC4752
0.215,700BP + RPF SP 37MC4799
0.185,550Rapid StaphMC4805
0.658,100BP + RPF PP 37MC4851
0.406,600BP + RPF PP 37MC4870
0.175,500BP SP 37MC4934
0.507,200BP SP 37MC5009
0.195,600BP SP 37MC5026
-0.084,500BP SP 37MC5027
-1.201,818BP SP 37MC5094
-0.234,000BP + RPF SP 37MC5246
0.195,600BP SP 37MC5676
-0.054,600BP + RPF PP 37MC5683
0.034,900BP + RPF SP 37MC5892
0.286,000OtherMC5969
-0.204,100OtherMC5989
0.004,800BP SP 37MC6008
-0.144,300OtherMC6008
-2.79506OtherMC6255
0.105,200BP SP 37MC6258
0.436,800BP + RPF PP 37MC6268
-0.054,600BP SP 37MC6295
0.698,400BP SP 37MC6421
0.436,800BP SP 37MC6446
-0.114,400OtherMC6447
0.085,100BP + RPF PP 37MC6476
-0.543,100OtherMC6524
-0.204,100PetrifilmMC6666
-0.433,400PetrifilmMC6679
0.406,600BP SP 37MC6722
<100BP SP 37MC6778
0.286,000BP SP 37MC6798
0.085,100BP SP 37MC6903
0.326,230BP SP 37MC6976
-0.433,400BP SP 37MC7050
0.004,800BP SP 37MC7099
0.527,300BP SP 37MC7112
0.175,500BP SP 37MC7189
-1.092,000PetrifilmMC7259
0.286,000BP SP 37MC7288
-0.114,400BP SP 37MC7289
-0.234,000BP SP 37MC7409
-0.034,700BP SP 37MC7419
0.085,100BP + RPF SP 37MC7486
-0.234,000BP SP 37MC7490
0.125,300BP SP 37MC7495
0.326,200OtherMC7592
0.155,400BP SP 37MC7593
0.175,500BP SP 37MC7594
0.175,500BP + RPF PP 37MC7729
Page 91 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.583,000BP SP 37MC7783
0.386,500BP SP 37MC7937
-1.062,045BP SP 37MC7955
-0.972,200PetrifilmMC7992
-0.583,000BP SP 37MC8152
0.195,600BP SP 37MC8312
0.326,200BP + RPF PP 37MC8949
0.155,400BP SP 37MC8949
Value
Data Statistics
191Number of Results
3Number of Excluded Results
3.67 log10Mean
3.68 log10Median
0.21 log10Standard Deviation
0.12 log10Robust Standard Deviation
506 to 63000 cfu/gResult Range
Performance Statistics
Value
4,800 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
958 to 24,057 cfu/gSatisfactory Range
96.8%Satisfactory z scores
2.1%Questionable z scores
1.1%Unsatisfactory z scores
Page 92 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Coagulase positive staphylococci
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
95.2580 to 630000.113.7043.98284BP SP 37
100.03800 to 73000.083.669.42018BP + RPF SP 37
100.03000 to 81000.113.7219.9038BP + RPF PP 37
100.02500 to 65000.143.682.0904Rapid Staph
94.7800 to 64000.193.549.95019Petrifilm
96.3506 to 100000.163.6414.66128Other
96.8506 to 630000.123.681003191All
Comments
The sample contained Staphylococcus aureus at an assigned value of 4,800 cfu/g.
The most significant food borne coagulase positive staphylococcus is Staphylococcus aureus. Staphylococcus aureus can be present onthe skin, and in the nose and throat of healthy individuals with no ill effect. It is important to test for it in foods as it can be an indicator ofgeneral hygiene and, and be a causative agent of food poisoning.
Page 93 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Bacillus species
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.006,700MYP SP 30MC1149
0.7212,000MYP SP 30MC1675
0.167,600MYP SP 30MC5027
-0.644,000MYP SP 30MC6722
-0.036,550MYP SP 30MC8312
Due to the low number of results returned, performance scores are shown for information purposes only
Value
Data Statistics
5Number of Results
0Number of Excluded Results
3.84 log10Mean
3.83 log10Median
0.17 log10Standard Deviation
0.08 log10Robust Standard Deviation
4000 to 12000 cfu/gResult Range
Performance Statistics
Value
6,700 cfu/gAssigned Value
0.05 log10Uncertainty of Assigned Value
0.35 log10SDPA
1,337 to 33,580 cfu/gSatisfactory Range
100.0%Satisfactory z scores
0.0%Questionable z scores
0.0%Unsatisfactory z scores
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.04000 to12000
0.083.8310005MYP SP 30
100.04000 to12000
0.083.8310005All
Comments
The sample contained Bacillus cereus at an assigned value of 6,700 cfu/g.
Bacillus is a large genus of Gram-positive bacilli that are aerobic, facultatively anaerobic and catalase positive. Bacillus species arewidespread in the environment, and are able to survive in adverse conditions due to the formation of endospores. They are able to survivein heat-processed and dried foods. Many species are normally present in soil and decaying animal and vegetable matter. Cells of bacilli arelarge and commonly adhere in chains.
Page 94 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.018,400OtherMC0123
-0.087,766BC SP 30MC0136
-0.714,700OtherMC0151
-0.495,600MYP SP 30MC0307
-0.406,000MYP SP 30MC0390
0.119,100OtherMC0431
0.068,700MYP SP 30MC0434
0.048,600MYP SP 30MC0437
0.048,600MYP SP 30MC0440
0.5613,000OtherMC0440
-0.147,400MYP SP 30MC0483
0.229,900MYP SP 30MC0854
-1.542,400MYP SP 30MC0914
0.2910,500BC SP 30MC0960
-0.515,500MYP SP 30MC1065
0.179,500MYP SP 30MC1096
0.018,400BC SP 30MC1097
0.6013,500MYP SP 30MC1105
-0.406,000PEMBA SP 30MC1149
0.2310,000MYP SP 30MC1226
0.008,300MYP SP 30MC1426
-0.187,200MYP SP 30MC1449
-0.097,700OtherMC1449
0.2910,500PEMBA SP 30MC1450
0.4612,000OtherMC1483
0.3511,000MYP SP 30MC1483
0.199,700MYP SP 30MC1493
-1.073,500MYP SP 30MC1533
-0.256,800MYP SP 30MC1538
-0.187,200MYP SP 30MC1601
0.229,900OtherMC1601
0.149,300MYP SP 30MC1602
-0.406,000OtherMC1619
<100OtherMC1653
-4.62200BC SP 30MC1653
0.4612,000MYP SP 30MC1675
0.2310,000MYP SP 30MC1684
0.159,400MYP SP 30MC1721
0.3511,000MYP SP 30MC1727
-0.028,200MYP SP 30MC1876
-0.217,000PEMBA SP 30MC1891
-0.286,600MYP SP 30MC1920
0.2310,000OtherMC1946
0.2610,200OtherMC2178
0.3511,000MYP SP 30MC2184
1.0920,000MYP SP 30MC2209
0.3511,000Compass BC SP 30MC2214
-0.306,500MYP SP 30MC2220
-0.306,500BC SP 30MC2220
-0.346,300MYP SP 30MC2240
0.7315,000OtherMC2262
0.219,800BC SP 30MC2279
-0.734,614MYP SP 30MC2321
0.4612,000BC SP 30MC2396
0.2310,000MYP SP 30MC2468
0.6914,500BC SP 30MC2510
-1.772,000OtherMC2588
0.189,600MYP SP 30MC2647
-0.635,000MYP SP 30MC2813
0.5613,000OtherMC2850
0.068,700MYP SP 30MC2851
Page 95 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.008,300OtherMC2851
-0.167,300BC SP 30MC2868
0.2310,000MYP SP 30MC3000
0.008,300MYP SP 30MC3102
-0.914,000BC SP 30MC3111
0.068,700MYP SP 30MC3228
-0.445,800OtherMC3242
-0.058,000MYP SP 30MC3243
0.139,200MYP SP 30MC3244
0.109,000MYP SP 30MC3245
-0.276,700BC SP 30MC3246
0.109,000MYP SP 30MC3248
0.3511,000OtherMC3249
-0.366,200MYP SP 30MC3250
-0.147,400MYP SP 30MC3251
-0.565,300OtherMC3252
0.4612,000PEMBA SP 30MC3253
-0.147,400OtherMC3254
-0.734,600MYP SP 30MC3255
-0.197,100MYP SP 30MC3256
-0.306,500MYP SP 30MC3392
-0.028,200BC SP 30MC3415
-0.585,200BC SP 30MC3465
0.119,100BC SP 30MC3471
0.149,300MYP SP 30MC3493
-0.087,800MYP SP 30MC3591
-0.605,100MYP SP 30MC3744
0.4612,000MYP SP 30MC3821
0.219,818Compass BC SP 30MC3824
3.37125,000OtherMC3973
-0.346,300MYP SP 30MC3990
0.7315,000MYP SP 30MC4067
0.4612,000OtherMC4079
-0.605,100Compass BC SP 30MC4174
-0.565,300OtherMC4262
-0.276,700MYP SP 30MC4296
-0.326,400OtherMC4339
0.008,300MYP SP 30MC4364
0.159,400MYP SP 30MC4369
-0.276,700OtherMC4372
0.4612,000MYP SP 30MC4584
0.4612,000OtherMC4584
-0.217,000MYP SP 30MC4672
0.3511,000MYP SP 30MC4688
0.068,700MYP SP 30MC4710
0.008,300OtherMC4710
-1.392,700MYP SP 30MC4752
0.5613,000MYP SP 30MC4799
0.2510,150Compass BC SP 30MC4805
-0.147,400MYP SP 30MC4851
0.038,500OtherMC4882
0.4612,000MYP SP 30MC5026
-0.684,800MYP SP 30MC5027
-0.724,636MYP SP 30MC5094
-0.217,000MYP SP 30MC5246
-0.147,400MYP SP 30MC5676
-0.187,200MYP SP 30MC5892
-0.028,200MYP SP 30MC5989
0.008,300OtherMC6008
-0.058,000MYP SP 30MC6008
<100PEMBA SP 30MC6255
Page 96 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.714,700BC SP 30MC6258
0.5613,000BC SP 30MC6268
0.8116,000MYP SP 30MC6295
0.5613,000MYP SP 30MC6421
-0.187,200OtherMC6446
-0.654,900OtherMC6447
-0.087,800MYP SP 30MC6666
-0.058,000MYP SP 30MC6679
-0.914,000MYP SP 30MC6722
-4.12300MYP SP 30MC6778
0.4612,000MYP SP 30MC6798
-2.87820PEMBA SP 30MC6903
0.098,900MYP SP 30MC6976
0.068,700MYP SP 30MC7050
-0.058,000MYP SP 30MC7099
0.2310,000MYP SP 30MC7112
-0.565,300MYP SP 30MC7189
0.219,800MYP SP 30MC7259
-0.445,800MYP SP 30MC7288
0.109,000MYP SP 30MC7289
-1.043,600MYP SP 30MC7409
-0.058,000MYP SP 30MC7419
0.109,000MYP SP 30MC7490
0.008,300MYP SP 30MC7593
-0.137,500MYP SP 30MC7729
0.109,000MYP SP 30MC7937
-2.78885MYP SP 30MC7955
0.3511,000MYP SP 30MC8152
-0.296,550MYP SP 30MC8312
-0.276,700MYP SP 30MC8949
Value
Data Statistics
152Number of Results
2Number of Excluded Results
3.88 log10Mean
3.92 log10Median
0.28 log10Standard Deviation
0.14 log10Robust Standard Deviation
200 to 125000 cfu/gResult Range
Performance Statistics
Value
8,300 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.35 log10SDPA
1,656 to 41,599 cfu/gSatisfactory Range
96.7%Satisfactory z scores
1.3%Questionable z scores
2.0%Unsatisfactory z scores
Page 97 of 123Issue: 1 09 October 2018
Sample: 17F - Staph/Bacillus
Analyte: Bacillus cereus
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
97.9300 to 200000.123.9263.16096MYP SP 30
80.0820 to 120000.263.853.9516PEMBA SP 30
100.05100 to11000
0.044.002.6304Compass BC SP30
93.8200 to 145000.153.9010.53016BC SP 30
96.62000 to125000
0.183.9219.74130Other
96.7200 to125000
0.143.921002152All
Comments
The sample contained Bacillus cereus at an assigned value of 8,300 cfu/g.
In foodstuffs, Bacillus cereus is both a spoilage organism and a causative agent of food poisoning. The organism is commonly found in arange of foods, including rice, eggs, bread, meat, milk and other dairy products.
Page 98 of 123Issue: 1 09 October 2018
Analyte: Escherichia coli O157
Sample: 22F - E.coli 0157
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedELISAMC0307
DetectedBROTH + CT-SMACMC0456
DetectedIMS + CT-SMACMC1019
DetectedVIDASMC1065
DetectedIMS + CT-SMACMC1510
DetectedPCRMC1576
DetectedIMS + CT-SMACMC1588
DetectedBROTH + CT-SMACMC1696
DetectedIMS + CT-SMACMC1796
DetectedVIDASMC1935
DetectedVIDASMC2000
DetectedBROTH + CT-SMACMC2169
DetectedVIDASMC2178
DetectedVIDASMC2209
DetectedVIDASMC2262
DetectedPCRMC2577
DetectedPCRMC2727
DetectedPCRMC2742
DetectedBROTH + CT-SMACMC2905
DetectedOtherMC3002
DetectedIMS + CT-SMACMC3102
DetectedOtherMC3102
DetectedIMS + CT-SMACMC3109
DetectedPCRMC3113
DetectedPCRMC3248
DetectedVIDASMC3457
DetectedREVEALMC3462
DetectedPCRMC3471
DetectedPCRMC3746
DetectedDuPont BAXMC3882
DetectedBROTH + CT-SMACMC4005
DetectedVIDASMC4079
DetectedBROTH + CT-SMACMC4296
DetectedOtherMC4372
DetectedIMS + CT-SMACMC4680
DetectedOtherMC4680
DetectedOtherMC4689
DetectedDuPont BAXMC4691
DetectedPCRMC4709
DetectedVIDASMC4721
DetectedBROTH + CT-SMACMC4722
DetectedDuPont BAXMC4722
DetectedPCRMC4774
DetectedVIDASMC4852
DetectedBROTH + CT-SMACMC4870
DetectedIMS + CT-SMACMC4943
DetectedIMS + CT-SMACMC4975
DetectedOtherMC5600
DetectedIMS + CT-SMACMC5695
DetectedBROTH + CT-SMACMC5937
DetectedVIDASMC5939
DetectedOtherMC5944
DetectedPCRMC5969
DetectedIMS + CT-SMACMC5979
DetectedPCRMC5979
DetectedBROTH + CT-SMACMC6353
DetectedPCRMC6353
DetectedVIDASMC6353
DetectedIMS + CT-SMACMC6411
DetectedBROTH + CT-SMACMC6411
DetectedBROTH + CT-SMACMC6446
DetectedBROTH + CT-SMACMC6447
Issue: 1 Page 99 of 123 09 October 2018
Analyte: Escherichia coli O157
Sample: 22F - E.coli 0157
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedBROTH + CT-SMACMC6526
Not DetectedREVEALMC6664
Detected3M Molecular Detection SystemMC6669
DetectedELISAMC6722
DetectedVIDASMC6737
Not DetectedPCRMC6750
DetectedELISAMC6767
DetectedNeogen RevealMC7057
DetectedIMS + CT-SMACMC7057
DetectedOtherMC7057
DetectedPCRMC7091
DetectedVIDASMC7255
DetectedDuPont BAXMC7291
DetectedOtherMC7291
DetectedRomer Rapid ChekMC7661
DetectedPCRMC7760
DetectedIMS + CT-SMACMC7905
Detected3M Molecular Detection SystemMC7983
Detected3M Molecular Detection SystemMC7992
Detected3M Molecular Detection SystemMC7998
Detected3M Molecular Detection SystemMC8026
DetectedDuPont BAXMC8062
DetectedOtherMC8231
Detected3M Molecular Detection SystemMC8240
DetectedDuPont BAXMC8822
DetectedOtherMC8822
DetectedVIDASMC9508
DetectedIMS + CT-SMACMC9572
Result
Data Statistics
DetectedAssigned Value
90Number of Results
98%Satisfactory
Methodology Summary
% SatisfactoryMethod
100%BROTH + CT-SMAC
100%IMS + CT-SMAC
50%REVEAL
94%PCR
100%ELISA
100%VIDAS
100%3M Molecular Detection System
100%DuPont BAX
100%Romer Rapid Chek
100%Neogen Reveal
100%Other
Comments
The sample contained Escherichia coli O157 (non-toxigenic strain) at an approximate level of 117 cfu/g. The assigned value is therefore Escherichia coli O157 ‘detected’.
Escherichia coli belongs to the Enterobacteriaceae family and the coliform group of indicator organisms. Cells of this organism are typicallyGram-negative rods that are motile, oxidase negative, produce gas from lactose at 37 and 44°C, possess the enzyme β-glucuronidase andproduce indole from tryptophan at 44°C. Some strains of Escherichia coli O157, in contrast, do not grow at 44°C and do not possess theβ-glucuronidase enzyme, so their presence may not be detected on chromogenic agars and in standard laboratory isolation procedures.Differentiation of Escherichia coli O157 from most other strains of Escherichia coli can usually be achieved using sorbitol MacConkey agar,as most strains of Escherichia coli O157 do not ferment sorbitol. There are also commercially available kits for confirmation of suspectcolonies, including latex agglutination kits.
Issue: 1 Page 100 of 123 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
1.0225,000YGC PP 22-25MC0009
1.1126,820PetrifilmMC0044
0.5617,207OtherMC0151
-0.0210,800RB SP 22-25MC0189
-0.417,900RB PP 22-25MC0192
-0.696,300YGC PP 22-25MC0214
-0.865,483YGC SP 22-25MC0249
1.1127,000DG18 SP 25MC0261
1.3131,500RB PP 22-25MC0261
-1.084,600PetrifilmMC0349
1.6341,000OtherMC0349
0.4616,000YGC SP 22-25MC0390
0.4616,000OGYE SP 22-25MC0495
0.6118,000OtherMC0822
0.5417,000DRBC PP 22-25MC0888
-1.084,600YGC PP 22-25MC0914
0.1212,100OtherMC0920
0.3815,000PetrifilmMC1033
0.0011,000DRBC PP 22-25MC1065
-0.259,000OtherMC1082
-0.567,000PetrifilmMC1085
-0.229,225OGYE PP 22-25MC1093
0.2113,000PetrifilmMC1130
-0.259,000YGC PP 22-25MC1191
-1.004,900PetrifilmMC1261
0.1112,000YGC SP 22-25MC1283
2.2668,000OtherMC1354
2.5989,000YGC SP 22-25MC1354
0.2113,000OtherMC1467
0.4616,000PetrifilmMC1481
-0.626,700YGC SP 22-25MC1520
-0.726,160YGC PP 22-25MC1580
0.3014,000YGC SP 22-25MC1611
0.0011,000YGC SP 22-25MC1640
-0.567,000PetrifilmMC1865
-0.756,000YGC PP 22-25MC1865
-0.239,150OtherMC1918
0.7420,000YGC SP 22-25MC1923
0.3815,000DG18 SP 25MC1937
-0.368,200DG18 SP 25MC1970
0.2113,000YGC PP 22-25MC1997
-0.676,400OGYE PP 22-25MC2061
0.6017,900OtherMC2149
0.3815,000MEA SP 22-25MC2269
1.6642,000YGC PP 22-25MC2287
0.7420,000DG18 SP 25MC2409
-3.42700RB PP 22-25MC2413
-0.666,450OtherMC2438
-0.179,600OGYE SP 22-25MC2468
-0.437,800RB SP 22-25MC2481
-0.149,800DG18 PP 25MC2612
-0.0110,900DRBC SP 22-25MC2612
0.1212,100YGC PP 22-25MC2612
0.1812,750OtherMC2668
0.0711,640YGC SP 22-25MC2769
0.1112,000YGC PP 22-25MC2860
-0.0710,414OtherMC2892
-0.955,100YGC PP 22-25MC2988
-1.264,000DG18 SP 25MC2988
0.5417,000YGC PP 22-25MC3000
-0.169,700DRBC SP 22-25MC3000
Page 101 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.0010,983RB SP 22-25MC3119
-0.825,700OtherMC3242
0.0011,000MEA SP 22-25MC3254
0.0011,000OtherMC3254
0.2713,700OGYE PP 22-25MC3306
0.2113,000YGC PP 22-25MC3497
-0.249,100YGC PP 22-25MC3499
1.7545,000YGC PP 22-25MC3517
-0.497,425DG18 SP 25MC3564
-0.0210,850OtherMC3661
0.0011,000OGYE SP 22-25MC3714
0.0011,000RB SP 22-25MC3731
0.3815,000OtherMC3746
-0.447,700DRBC PP 22-25MC3921
-0.328,500YGC PP 22-25MC4015
0.0311,300YGC PP 22-25MC4077
0.0811,700RB SP 22-25MC4296
-0.865,500DG18 SP 25MC4356
0.2113,000OtherMC4372
-0.756,000YGC SP 22-25MC4398
-0.229,226YGC PP 22-25MC4424
-0.447,727OGYE PP 22-25MC4648
-0.567,000DG18 SP 25MC4649
0.0011,000PetrifilmMC4649
-0.696,300OGYE PP 22-25MC4774
0.2113,000DRBC SP 22-25MC4852
0.4515,750RB PP 22-25MC4879
0.0011,000OGYE PP 22-25MC4975
0.0011,000YGC SP 22-25MC5008
-0.0610,500OGYE SP 22-25MC5055
<10YGC PP 22-25MC5510
-0.179,600OtherMC5562
-1.702,800DG18 SP 25MC5566
-1.423,500YGC PP 22-25MC5566
-0.378,133YGC PP 22-25MC5674
-0.1110,035DG18 PP 25MC5683
<10OtherMC5690
-0.0510,600DRBC PP 22-25MC5705
-0.1210,020YGC PP 22-25MC5769
0.0911,820YGC PP 22-25MC5809
0.6218,100OtherMC5910
0.1712,600YGC PP 22-25MC5949
0.3314,330DG18 PP 25MC5954
0.3014,000DRBC PP 22-25MC5954
0.2113,000OtherMC5969
0.1112,000PetrifilmMC6008
0.8021,000OtherMC6008
-0.259,000OtherMC6253
0.0011,000RB PP 22-25MC6559
0.6518,500PetrifilmMC6572
0.8021,000OtherMC6834
-0.765,940OtherMC7296
-3.01970YGC PP 22-25MC7370
-0.219,300YGC SP 22-25MC7411
0.3414,500YGC SP 22-25MC7609
0.2113,000YGC PP 22-25MC7611
0.0011,000DG18 SP 25MC7658
-0.1210,000DRBC SP 22-25MC7732
-0.537,200RB SP 22-25MC7775
-0.1210,000DRBC SP 22-25MC7862
-0.189,540YGC PP 22-25MC7956
Page 102 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.0011,000DRBC SP 22-25MC7992
4.06290,000DG18 SP 25MC8048
0.3414,500YGC SP 22-25MC8395
-0.408,000PetrifilmMC8845
0.3014,000PetrifilmMC8846
-0.696,300PetrifilmMC8847
-0.567,000OtherMC8847
-0.358,300OtherMC8848
-0.626,700PetrifilmMC8848
<10PetrifilmMC8849
2.1160,000PetrifilmMC9018
-0.408,000PetrifilmMC9102
0.0211,150OtherMC9112
-0.437,800OtherMC9217
-4.70250OtherMC9234
0.0211,170PetrifilmMC9244
0.2012,900PetrifilmMC9250
-3.06935OtherMC9268
0.3014,000OtherMC9289
0.2213,100OtherMC9382
-0.149,800DG18 SP 25MC9398
-0.189,500OtherMC9560
-0.318,600OtherMC9561
0.0111,100DG18 SP 25MC9572
0.0211,200PetrifilmMC9581
Value
Data Statistics
147Number of Results
3Number of Excluded Results
4.02 log10Mean
4.04 log10Median
0.33 log10Standard Deviation
0.18 log10Robust Standard Deviation
250 to 290000 cfu/gResult Range
Performance Statistics
Value
11,000 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
2,195 to 55,131 cfu/gSatisfactory Range
94.4%Satisfactory z scores
2.1%Questionable z scores
3.5%Unsatisfactory z scores
Page 103 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.09600 to16000
0.044.032.7204OGYE SP 22-25
92.32800 to290000
0.273.998.84013DG18 SP 25
100.09800 to14330
0.024.002.0403DG18 PP 25
100.011000 to15000
0.104.111.3602MEA SP 22-25
100.07200 to11700
0.034.044.0806RB SP 22-25
100.09700 to13000
0.034.024.0806DRBC SP 22-25
100.07700 to17000
0.164.043.405DRBC PP 22-25
100.06300 to13700
0.173.934.0806OGYE PP 22-25
80.0700 to 315000.234.043.405RB PP 22-25
92.95483 to89000
0.144.079.52014YGC SP 22-25
96.2970 to 450000.183.9918.37127YGC PP 22-25
95.04600 to60000
0.224.0514.29121Petrifilm
91.2250 to 680000.174.0423.81135Other
94.4250 to290000
0.184.041003147All
Comments
The sample contained the yeast Saccharomyces cerevisiae at an assigned value of 11,000 cfu/g.
Yeasts are simply fungi whose main growth form is unicellular and which usually replicate by budding.
Page 104 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.597,100YGC PP 22-25MC0009
0.114,805PetrifilmMC0044
-1.261,590OtherMC0151
0.546,800RB SP 22-25MC0189
0.546,800RB PP 22-25MC0192
0.285,500YGC PP 22-25MC0214
0.034,500YGC SP 22-25MC0249
-0.562,800DG18 SP 25MC0261
-0.522,900RB PP 22-25MC0261
-0.522,900PetrifilmMC0349
-0.124,000OtherMC0349
1.2111,667YGC SP 22-25MC0390
-0.433,100OGYE SP 22-25MC0495
0.064,600OtherMC0822
0.004,400DRBC PP 22-25MC0888
0.255,400YGC PP 22-25MC0914
-1.111,800OtherMC0920
0.587,000PetrifilmMC1033
0.969,500DRBC PP 22-25MC1065
0.899,000OtherMC1082
-0.982,000PetrifilmMC1085
0.104,760OGYE PP 22-25MC1093
0.899,000PetrifilmMC1130
0.114,800YGC PP 22-25MC1191
-1.041,900PetrifilmMC1261
-1.421,400YGC SP 22-25MC1283
-0.862,200OtherMC1354
-0.562,800YGC SP 22-25MC1354
0.416,100OtherMC1467
0.748,000PetrifilmMC1481
1.1310,900YGC SP 22-25MC1520
-0.054,230YGC PP 22-25MC1580
-1.341,500YGC SP 22-25MC1611
-0.483,000YGC SP 22-25MC1640
-0.483,000PetrifilmMC1865
-0.124,000YGC PP 22-25MC1865
0.124,850OtherMC1918
0.134,900YGC SP 22-25MC1923
-1.341,500DRBC PP 22-25MC1937
-1.421,400DG18 SP 25MC1970
0.365,900YGC PP 22-25MC1997
0.878,850OGYE PP 22-25MC2061
-0.982,000OtherMC2149
-0.223,700MEA SP 22-25MC2269
-0.483,000YGC PP 22-25MC2287
0.386,000DG18 SP 25MC2409
-1.97900RB SP 22-25MC2413
0.627,250OtherMC2438
0.034,500OGYE SP 22-25MC2468
-0.982,000OGYE PP 22-25MC2468
0.657,400RB SP 22-25MC2481
0.526,700DG18 PP 25MC2612
0.566,900DRBC SP 22-25MC2612
0.416,100YGC PP 22-25MC2612
0.295,550OtherMC2668
<10YGC SP 22-25MC2769
-0.034,300YGC PP 22-25MC2860
-0.682,550OtherMC2892
-1.111,800DG18 SP 25MC2988
-1.341,500YGC PP 22-25MC2988
0.386,000YGC PP 22-25MC3000
Page 105 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.325,700DRBC SP 22-25MC3000
0.094,733RB SP 22-25MC3119
0.064,600OtherMC3242
0.386,000MEA SP 22-25MC3254
0.386,000OtherMC3254
-0.183,800OGYE PP 22-25MC3306
-0.183,800YGC PP 22-25MC3497
0.215,200YGC PP 22-25MC3499
0.486,500YGC PP 22-25MC3517
1.6817,100DG18 SP 25MC3564
0.215,200OtherMC3661
0.084,700OGYE SP 22-25MC3714
0.185,100RB SP 22-25MC3731
-0.483,000OtherMC3746
-0.562,800DRBC PP 22-25MC3921
-0.612,700YGC PP 22-25MC4015
0.386,000YGC PP 22-25MC4077
0.235,300RB SP 22-25MC4296
-1.721,100DG18 SP 25MC4356
1.1411,000OtherMC4372
0.748,000YGC SP 22-25MC4398
-2.87435YGC PP 22-25MC4424
0.466,364OGYE SP 22-25MC4648
0.667,500PetrifilmMC4649
0.899,000DG18 SP 25MC4649
-0.982,000OGYE PP 22-25MC4774
0.768,100DRBC SP 22-25MC4852
0.315,650RB PP 22-25MC4879
-0.034,300OGYE PP 22-25MC4975
-0.802,300YGC SP 22-25MC5008
0.899,000OGYE SP 22-25MC5055
<10YGC PP 22-25MC5510
-0.752,400OtherMC5562
-0.752,400DG18 SP 25MC5566
-0.522,900YGC PP 22-25MC5566
-1.181,700YGC PP 22-25MC5674
0.185,100DG18 PP 25MC5683
1.1411,000OtherMC5690
0.034,500DRBC SP 22-25MC5705
-0.313,420YGC SP 22-25MC5769
0.627,270YGC PP 22-25MC5809
-0.044,250OtherMC5910
0.587,000YGC PP 22-25MC5949
0.165,000DG18 PP 25MC5954
0.386,000DRBC PP 22-25MC5954
-0.153,900OtherMC5969
-0.562,800PetrifilmMC6008
-0.483,000OtherMC6008
-0.094,100OtherMC6253
0.486,500RB PP 22-25MC6559
0.165,000PetrifilmMC6572
-0.253,600OtherMC6834
-0.213,727OtherMC7296
-3.07370YGC PP 22-25MC7370
-1.841,000YGC PP 22-25MC7411
1.8219,000YGC SP 22-25MC7609
0.587,000YGC PP 22-25MC7611
-0.124,000DG18 SP 25MC7658
0.899,000DRBC PP 22-25MC7732
0.004,400RB SP 22-25MC7775
0.044,545DRBC SP 22-25MC7862
Page 106 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.223,700YGC PP 22-25MC7956
-0.064,200DRBC SP 22-25MC7992
0.114,800DG18 SP 25MC8048
0.285,500YGC SP 22-25MC8395
0.165,000PetrifilmMC8845
0.587,000PetrifilmMC8846
0.386,000PetrifilmMC8847
0.004,400OtherMC8847
-0.483,000OtherMC8848
-0.153,900PetrifilmMC8848
-0.982,000PetrifilmMC8849
3.3264,000PetrifilmMC9018
<10PetrifilmMC9102
-0.782,350OtherMC9112
-1.181,700OtherMC9217
-2.12800OtherMC9234
-1.97900PetrifilmMC9244
-0.363,300PetrifilmMC9250
-3.51260OtherMC9268
0.255,400OtherMC9289
-0.034,300OtherMC9382
-0.612,700DG18 SP 25MC9398
-0.922,100OtherMC9560
-1.511,300OtherMC9561
-0.034,300DG18 SP 25MC9572
-1.611,200PetrifilmMC9581
Value
Data Statistics
148Number of Results
3Number of Excluded Results
3.59 log10Mean
3.64 log10Median
0.32 log10Standard Deviation
0.25 log10Robust Standard Deviation
260 to 64000 cfu/gResult Range
Performance Statistics
Value
4,400 cfu/gAssigned Value
0.03 log10Uncertainty of Assigned Value
0.35 log10SDPA
878 to 22,052 cfu/gSatisfactory Range
96.6%Satisfactory z scores
1.4%Questionable z scores
2.1%Unsatisfactory z scores
Page 107 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Mould
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.03100 to 90000.203.673.3805OGYE SP 22-25
100.01100 to17100
0.303.528.11012DG18 SP 25
100.05000 to 67000.013.712.0303DG18 PP 25
100.03700 to 60000.163.671.3502MEA SP 22-25
100.0900 to 74000.103.714.7307RB SP 22-25
100.04200 to 81000.103.714.0506DRBC SP 22-25
100.01500 to 95000.383.714.0506DRBC PP 22-25
100.02000 to 88500.283.614.0506OGYE PP 22-25
100.02900 to 68000.063.782.704RB PP 22-25
100.01400 to19000
0.373.659.46114YGC SP 22-25
92.3370 to 72700.213.6618.24127YGC PP 22-25
95.0900 to 640000.313.6414.19121Petrifilm
94.3260 to 110000.273.5923.65035Other
96.6260 to 640000.253.641003148All
Comments
The sample contained the mould Penicillium roquefortii at an assigned value of 4,400 cfu/g.
Visible mould growth can often be seen on foods, such as bread and vegetables, and in the environment, such as in bathrooms and airconditioning units. Many fungi are saprophytes, causing spoilage of foods and other commodities.
Page 108 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast & Mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.9434,000YGC PP 22-25MC0009
-0.2313,300PetrifilmMC0144
-0.0814,970OGYE SP 22-25MC0208
-0.0815,000RB SP 22-25MC0473
0.4322,600OtherMC0822
0.6527,000DRBC SP 22-25MC0959
-0.2413,200YGC PP 22-25MC1059
0.3120,500DRBC PP 22-25MC1065
-0.1813,800YGC PP 22-25MC1191
-0.2213,400YGC SP 22-25MC1283
0.1217,600YGC SP 22-25MC1520
-0.5810,000PetrifilmMC1865
-0.5810,000YGC PP 22-25MC1865
-0.1714,000OtherMC1918
0.5524,900YGC PP 22-25MC1923
0.4523,000DRBC SP 22-25MC1937
0.2619,800OtherMC2149
1.2845,000YGC PP 22-25MC2287
-0.1913,700YGC PP 22-25MC2438
-0.2612,964OtherMC2892
-1.037,000DG18 SP 25MC2988
-1.106,600YGC PP 22-25MC2988
0.4523,000YGC PP 22-25MC3000
-0.0815,000DRBC SP 22-25MC3000
0.8632,000YGC PP 22-25MC3173
0.0817,000MEA SP 22-25MC3254
0.0817,000OtherMC3254
0.1117,500OGYE PP 22-25MC3306
0.0616,800YGC PP 22-25MC3497
-0.1714,000YGC PP 22-25MC3499
0.5324,525DG18 SP 25MC3564
0.0016,000OGYE SP 22-25MC3714
-0.4611,000YGC PP 22-25MC4015
0.1017,300YGC PP 22-25MC4077
-0.639,661YGC PP 22-25MC4424
0.4523,000PetrifilmMC4458
-0.818,300OGYE PP 22-25MC4774
0.3421,100DRBC SP 22-25MC4852
-0.0815,000OGYE SP 22-25MC4975
-0.2313,300YGC PP 22-25MC5008
0.2519,500OGYE SP 22-25MC5055
-1.355,400DG18 SP 25MC5566
-1.126,500YGC PP 22-25MC5566
0.0116,100OtherMC5635
-0.609,833YGC PP 22-25MC5674
0.0616,785DG18 PP 25MC5683
-0.0914,900DRBC SP 22-25MC5705
-0.2213,440YGC PP 22-25MC5769
0.2219,100YGC PP 22-25MC5809
0.4122,350OtherMC5910
0.2519,600YGC PP 22-25MC5949
0.2319,330DG18 PP 25MC5954
0.2820,000DRBC PP 22-25MC5954
-0.0815,000PetrifilmMC6008
0.5024,000OtherMC6008
0.1117,500RB PP 22-25MC6559
0.4823,500PetrifilmMC6572
0.6527,000OtherMC6834
0.0016,000DRBC SP 22-25MC6865
0.0817,000PetrifilmMC6927
-3.081,340YGC PP 22-25MC7370
Page 109 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast & Mould
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
0.3421,000OtherMC7371
0.9233,500YGC SP 22-25MC7609
-0.0815,000DG18 SP 25MC7658
-0.1214,545DRBC SP 22-25MC7862
-0.2313,240YGC PP 22-25MC7956
-0.967,400OtherMC7992
-0.4411,200PetrifilmMC8065
0.2119,000PetrifilmMC8152
0.2820,000YGC SP 22-25MC8395
-0.5010,700PetrifilmMC8834
-0.5810,000DG18 PP 25MC8837
-0.4910,800PetrifilmMC8844
0.3421,000PetrifilmMC8846
<10PetrifilmMC8979
-0.2113,500OtherMC9035
0.0016,000OtherMC9036
-0.5310,400PetrifilmMC9064
0.0416,475DRBC PP 22-25MC9098
-0.2113,500OtherMC9112
-0.1014,800OtherMC9136
0.5024,000OtherMC9197
-0.2413,200OtherMC9201
-0.659,500OtherMC9217
-1.305,620OtherMC9248
-0.4011,600PetrifilmMC9252
0.2419,400OtherMC9289
-0.0715,100OtherMC9337
4.71712,000MEA SP 22-25MC9338
-0.0914,900PetrifilmMC9341
0.5024,000DRBC SP 22-25MC9368
0.5525,000YGC PP 22-25MC9368
0.5024,000PetrifilmMC9382
-0.1314,450MEA SP 22-25MC9401
-5.07270OtherMC9420
0.9434,000OtherMC9468
1.2845,000OtherMC9479
0.0016,000YGC SP 22-25MC9501
0.6026,000PetrifilmMC9550
-0.4011,600OtherMC9560
-0.0515,400DG18 SP 25MC9572
0.1818,500OtherMC9577
Value
Data Statistics
102Number of Results
2Number of Excluded Results
4.21 log10Mean
4.20 log10Median
0.26 log10Standard Deviation
0.14 log10Robust Standard Deviation
1340 to 712000 cfu/gResult Range
Performance Statistics
Value
16,000 cfu/gAssigned Value
0.02 log10Uncertainty of Assigned Value
0.35 log10SDPA
3,192 to 80,190 cfu/gSatisfactory Range
97.0%Satisfactory z scores
0.0%Questionable z scores
3.0%Unsatisfactory z scores
Page 110 of 123Issue: 1 09 October 2018
The sample contained the yeast Saccharomyces cerevisiae and the mould Penicillium roquefortii at an assigned value of 16,000 cfu/g.
Sample: 23D - Yeast & Mould
Analyte: Yeast & Mould
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
100.014970 to19500
0.024.193.9204OGYE SP 22-25
100.05400 to24525
0.324.184.905DG18 SP 25
100.010000 to19330
0.094.222.9403DG18 PP 25
66.714450 to712000
0.104.232.9403MEA SP 22-25
100.015000 to15000
0.004.180.9801RB SP 22-25
100.014545 to27000
0.144.267.8408DRBC SP 22-25
100.016475 to20500
0.024.302.9403DRBC PP 22-25
100.08300 to17500
0.244.081.9602OGYE PP 22-25
100.017500 to17500
0.004.240.9801RB PP 22-25
100.013400 to33500
0.084.254.905YGC SP 22-25
95.81340 to45000
0.214.1423.53024YGC PP 22-25
100.010000 to26000
0.214.1716.67117Petrifilm
96.25620 to45000
0.144.2125.49126Other
97.01340 to712000
0.144.201002102All
Comments
Page 111 of 123Issue: 1 09 October 2018
Sample: 23D - Yeast & Mould
Analyte: Yeast & Mould
Round: 269Scheme: Food Microbiology (QMS)
Fungi, including yeast and moulds, are ubiquitous throughout the environment, being found in air, water, soils, animals and foods. They cansurvive adverse conditions by the production of spores. Many fungi are saprophytes, causing spoilage of foods and other commodities.
Page 112 of 123Issue: 1 09 October 2018
Sample: 24F - Lactic Acid Bacteria
Analyte: Lactic acid bacteria
Round: 269Scheme: Food Microbiology (QMS)
Result (cfu/g)MethodLab ID
<10MRS PP 30MC0123
1,100MRS PP 30MC0247
950MRS PP 30MC0307
<100OtherMC0308
<10MRS PP 30MC0390
1,300OtherMC0456
<10AM SP 30MC0673
<10PetrifilmMC1009
1,536MRS PP 30MC1042
1,900MRS PP 30MC1065
<10PetrifilmMC1160
2,200MRS SP 30MC1181
2,200MRS SP 30MC1297
2,300MRS PP 30MC1387
3,300OtherMC1467
<10OtherMC1490
2,000MRS SP 30MC1630
8,000OtherMC1768
1,140MRS SP 30MC1926
8,900OtherMC1926
2,100MRS PP 25MC1997
<10MRS PP 30MC2000
2,000MRS PP 30MC2031
<10MRS PP 30MC2053
3,300MRS PP 30MC2107
<10MRS PP 30MC2109
1,970MRS SP 30MC2127
4,600MRS PP 30MC2255
<10OtherMC2262
<10MRS PP 30MC2294
<10MRS PP 30MC2345
1,600MRS PP 30MC2410
<100OtherMC2460
2,400MRS SP 30MC2479
650MRS PP 30MC2481
1,200MRS PP 30MC2522
2,100MRS PP 30MC2597
1,400MRS PP 30MC2988
1,600MRS SP 30MC2988
350MRS PP 30MC3000
2,130MRS PP 30MC3026
<10MRS PP 30MC3123
2,300MRS SP 30MC3180
<10MRS PP 30MC3242
<10OtherMC3288
1,730MRS PP 30MC3306
2,600MRS PP 30MC3358
2,900MRS PP 30MC3399
9,200MRS SP 30MC3465
1,400MRS PP 30MC3471
2,000MRS PP 30MC3679
2,370MRS PP 30MC3715
<10MRS PP 25MC3982
<10MRS PP 30MC4015
1,300OtherMC4023
720MRS SP 30MC4034
2,700MRS PP 30MC4079
2,200MRS PP 30MC4245
<10MRS SP 30MC4356
2,393MRS SP 30MC4424
<10PetrifilmMC4495
Page 113 of 123Issue: 1 09 October 2018
Sample: 24F - Lactic Acid Bacteria
Analyte: Lactic acid bacteria
Round: 269Scheme: Food Microbiology (QMS)
Result (cfu/g)MethodLab ID
<10MRS PP 30MC4624
<100MRS SP 30MC4633
<100OtherMC4633
1,800OtherMC4721
<10PetrifilmMC4722
1,143MRS PP 30MC4732
1,200MRS PP 30MC4736
5,800MRS PP 30MC4774
736MRS PP 30MC4791
<10MRS SP 30MC4827
2,500MRS SP 30MC4901
1,500MRS PP 30MC4918
<10MRS PP 30MC5178
1,000MRS PP 30MC5246
2,400MRS PP 30MC5531
1,100MRS PP 30MC5566
2,400MRS SP 30MC5566
<10OtherMC5615
3,950MRS SP 30MC5635
1,700MRS PP 30MC5861
1,800MRS SP 30MC5861
1,800MRS PP 30MC5944
<10PetrifilmMC5979
2,400MRS PP 30MC6234
1,900MRS PP 30MC6246
<10OtherMC6336
1,100MRS PP 30MC6404
2,000MRS SP 30MC6435
1,800MRS PP 30MC6436
<10MRS PP 30MC6446
<10MRS PP 30MC6447
<10PetrifilmMC6457
2,000MRS SP 30MC6550
2,000MRS SP 30MC6564
1,700PetrifilmMC6669
<10MRS PP 30MC6745
400MRS PP 25MC6834
2,700MRS PP 30MC6865
<10MRS PP 30MC6965
<10MRS PP 30MC7255
15OtherMC7612
1,940OtherMC7642
1,560MRS SP 30MC7775
<10PetrifilmMC7992
<10MRS PP 30MC8196
<10MRS PP 30MC8852
<10PetrifilmMC8852
4,700PetrifilmMC9043
1,300OtherMC9045
2,200OtherMC9086
<10PetrifilmMC9098
20PetrifilmMC9186
<10Rogosa PP 30MC9234
<10OtherMC9248
180Rogosa PP 30MC9268
<10PetrifilmMC9298
1,980OtherMC9382
2,500MRS PP 30MC9536
30PetrifilmMC9550
2,318MRS SP 30MC9572
Page 114 of 123Issue: 1 09 October 2018
Sample: 24F - Lactic Acid Bacteria
Analyte: Lactic acid bacteria
Round: 269Scheme: Food Microbiology (QMS)
Value
Data Statistics
121Number of Results
N/ANumber of Excluded Results
N/AMean
N/AMedian
N/AStandard Deviation
N/ARobust Standard Deviation
15 to 9,200 cfu/gResult Range
Performance Statistics
Value
AbsentAssigned Value
N/AUncertainty of Assigned Value
N/ASDPA
N/ASatisfactory Range
N/ASatisfactory z scores
N/AQuestionable z scores
N/AUnsatisfactory z scores
Comments
The sample contained the yeast Saccharomyces cerevisiae at an approximate level of 3,210 cfu/g. Lactic acid bacteria were not present in the sample therefore the assigned value is ‘absent’ using a detection level of <10 cfu/g.
Approximately 36% of participants correctly reported the absence of lactic acid bacteria from the sample. Due to the low number ofparticipants achieving the correct result internal quality control analysis was repeated on this test material. Results were consistent with theoriginal testing with the target organism being absent from all samples analysed. Enumeration results returned by participants wereconsistent with the level of yeast in the sample. Agars intended for the enumeration of lactic acid bacteria can also support the growth ofyeast; therefore participants should employ sufficient confirmatory techniques to ensure they have detected the target organism. In this casemicroscopy or a catalase test would have confirmed the absence of lactic acid bacteria. Participants gaining an incorrect result may wish toassess the significance of this internally.
Lactic acid bacteria are important in the food, brewing, and dairy industries where fermentation occurs due to their production of lactic andacetic acids. They are beneficial to health and are included in a number of probiotic foods. However, lactic acid bacteria can also causefood spoilage as high levels cause off flavours and odours and lead to a reduced shelf life. Lactic acid bacteria are often inhibitory to othermicro-organisms.
Page 115 of 123Issue: 1 09 October 2018
Analyte: Identification
Sample: 33 - Identification of unknown organism
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
Enterococcus faecalisBiochemicalMC0137
Gram-positive cocciOtherMC0199
Enterococcus faecalisGenotypicMC0440
Enterococcus faecalisProtein AnalysisMC0440
Enterococcus faecalisSpectometryMC0440
Gram-positive cocciBiochemicalMC1317
Enterococcus faecalisOtherMC1377
Enterococcus faecalisSpectometryMC1438
Enterococcus faecalisSpectometryMC1547
Enterococcus faecalisBiochemicalMC2315
Enterococcus faecalisMorphologicalMC2410
Enterococcus faecalisOtherMC3113
Enterococcus faecalisOtherMC3242
Staphylococcus sp.BiochemicalMC3574
Enterococcus faecalisOtherMC3591
Enterococcus faecalisOtherMC3840
Gram-positive cocciBiochemicalMC3882
Enterococcus faecalisSpectometryMC3923
Gram-positive cocciMorphologicalMC3923
Enterococcus faecalisBiochemicalMC3924
Enterococcus faecalisOtherMC4003
Enterococcus faecalisGenotypicMC4709
Gram-positive cocciMorphologicalMC4833
Gram-positive cocciMorphologicalMC6292
Enterococcus faecalisOtherMC6292
Enterococcus faecalisOtherMC6425
Gram-positive cocciMorphologicalMC6503
Gram-positive cocciOtherMC7660
Enterococcus faecalisGenotypicMC7832
Enterococcus faecalisOtherMC7909
Enterococcus faecalisBiochemicalMC8162
Gram-positive cocciBiochemicalMC8827
Enterococcus faecalisBiochemicalMC9578
Result
Data Statistics
Enterococcus faecalis,Gram-positivecocci,Enterococcus sp.
Assigned Value
33Number of Results
97%Satisfactory
Methodology Summary
% SatisfactoryMethod
100%Morphological
89%Biochemical
100%Protein Analysis
100%Genotypic
100%Spectometry
100%Other
Comments
The test material contained Enterococcus faecalis. The correct identifications could therefore have been any of the following; Enterococcusfaecalis, Gram-positive cocci, Enterococcus sp.
A number of methods may be employed for the final identification of an organism, ranging from a simple Gram stain and biochemicaltesting, to serological and molecular methods such as latex agglutination and PCR.
The strain of Enterococcus faecalis included in the test material is a reference strain, and is a typical Enterococci. The strain is aGram-positive cocci and catalase negative. This particular strain was originally isolated from baby faeces. The organism producescharacteristic black/grey colonies on kanamycin aesculin azide agar, with surrounding blackening of agar. The organism reacts withLancefield group D.
Issue: 1 Page 116 of 123 09 October 2018
Analyte: Salmonella Serogroup
Sample: 34 - Identification of Salmonella
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
BSerologicalMC2315
DSerologicalMC3645
BSerologicalMC3882
BSerologicalMC3923
BSerologicalMC3924
BSerologicalMC6760
BSerologicalMC6967
Result
Data Statistics
BAssigned Value
7Number of Results
86%Satisfactory
Methodology Summary
% SatisfactoryMethod
86%Serological
Comments
The sample contained Salmonella Stanley, which has the serogroup B.
The strain of Salmonella Stanley used in this test material was isolated from a case of food poisoning in 1917 and is typical of the genus. Itproduces characteristic colonies on Salmonella selective agars and typical reactions in biochemical tests. It is a contaminant of several foodproducts, including peanuts, seeds and various fruits and vegetables. The organism is a group B Salmonella and according to theKauffmann-White scheme serology is O antigen; 1,4,[5],12,27: H phase 1 antigen d: H phase 2 antigen 1,2.
Salmonella are classified into distinct groups according to the presence of various surface antigens using the Kauffman-White classificationsystem. Initial grouping is performed in accordance with the ‘O’ antigen (based on polysaccharides associated with lipopolysaccharide).Further typing is performed on the ‘H’ antigen according to flagella antigens.
Issue: 1 Page 117 of 123 09 October 2018
Analyte: Salmonella Serovar
Sample: 34 - Identification of Salmonella
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
Salmonella StanleySerologicalMC1939
Salmonella StanleySerologicalMC2410
Salmonella LivingstoneSerologicalMC3645
Salmonella StanleySerologicalMC3882
Salmonella StanleySerologicalMC3923
Salmonella StanleySerologicalMC3924
Salmonella StanleySerologicalMC6760
Salmonella StanleySerologicalMC6967
Salmonella StanleySerologicalMC9164
Salmonella EnteritidisOtherMC9605
Result
Data Statistics
Salmonella StanleyAssigned Value
10Number of Results
80%Satisfactory
Methodology Summary
% SatisfactoryMethod
89%Serological
0%Other
Comments
The correct serovar for the strain included in the sample is Salmonella Stanley.
The strain of Salmonella Stanley used in this test material was isolated from a case of food poisoning in 1917 and is typical of the genus. Itproduces characteristic colonies on Salmonella selective agars and typical reactions in biochemical tests. It is a contaminant of several foodproducts, including peanuts, seeds and various fruits and vegetables. The organism is a group B Salmonella and according to theKauffmann-White scheme serology is O antigen; 1,4,[5],12,27: H phase 1 antigen d: H phase 2 antigen 1,2.
Salmonella are classified into distinct groups according to the presence of various surface antigens using the Kauffman-White classificationsystem. Initial grouping is performed in accordance with the ‘O’ antigen (based on polysaccharides associated with lipopolysaccharide).Further typing is performed on the ‘H’ antigen according to flagella antigens.
Issue: 1 Page 118 of 123 09 October 2018
Sample: 35 - Colony count and calculation of number of microorganisms
Analyte: Enumeration of Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
z scoreResult (cfu/g)MethodLab ID
-0.14600,000Visual CountMC0063
-0.29590,000Visual CountMC0079
-0.22595,000Visual CountMC0261
-1.06540,000Visual CountMC0729
0.21625,000Visual CountMC1073
-0.29590,000Visual CountMC1538
-0.14600,000Visual CountMC2315
-0.44580,000Visual CountMC2988
0.00610,000Visual CountMC4310
-0.90550,000OtherMC4310
-0.44580,000Visual CountMC4833
-0.22595,000Visual CountMC4921
-1.06540,000Visual CountMC5566
-1.39520,000Visual CountMC6406
-0.90550,000OtherMC6496
-0.14600,000Visual CountMC6552
-0.29590,000Visual CountMC6590
-0.14600,000Visual CountMC6967
-20.7456,000Visual CountMC7332
-0.90550,000Visual CountMC7386
-0.74560,000Visual CountMC7387
-0.82555,000Visual CountMC7391
-0.90550,000Visual CountMC7392
-0.44580,000Visual CountMC7393
-0.14600,000Visual CountMC7634
0.00610,000Visual CountMC7660
-1.06540,000Visual CountMC7670
0.14620,000Visual CountMC8073
Value
Data Statistics
28Number of Results
1Number of Excluded Results
5.76 log10Mean
5.77 log10Median
0.02 log10Standard Deviation
0.02 log10Robust Standard Deviation
520000 to 625000 cfu/gResult Range
Performance Statistics
Value
610,000 cfu/gAssigned Value
0.01 log10Uncertainty of Assigned Value
0.05 log10SDPA
484,540 to 767,945 cfu/gSatisfactory Range
96.4%Satisfactory z scores
0.0%Questionable z scores
3.6%Unsatisfactory z scores
Page 119 of 123Issue: 1 09 October 2018
The paper exercise showed the images of two duplicate plates containing Clostridium perfringens colonies at a dilution of 10-4, using 1mlaliquots (see image below).
Sample: 35 - Colony count and calculation of number of microorganisms
Analyte: Enumeration of Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
Methodology Summary
%Log10TotalResultsResultsMethod
Sat.RangeRobust SDMedian% ofExcludedNumber of
96.2520000 to625000
0.015.7792.86126Visual Count
100.0550000 to550000
0.005.747.1402Other
96.4520000 to625000
0.025.77100128All
Comments
Page 120 of 123Issue: 1 09 October 2018
Sample: 35 - Colony count and calculation of number of microorganisms
Analyte: Enumeration of Clostridium perfringens
Round: 269Scheme: Food Microbiology (QMS)
The plates were prepared and photographed within the LGC laboratory. The assigned value of 610,000 cfu/g was determined internally byLGC based on counting the colonies on each plate and calculating the number of CFU in the original sample by taking the dilution andinoculum volume into account. The assigned value is therefore based on formulation.
For a result to be valid, in general it is considered that it is necessary to enumerate the CFU on at least one plate containing a minimum of15 CFU and not more than 300 CFU. The recommended counting limit may vary depending on the method employed, and participants areurged to consult the relevant standard if they are unsure. A total of 62 CFU were enumerated on the left hand plate and 60 CFU on the righthand plate.
The correct calculation in this instance would be to average the number of visible colonies on the duplicate plates and multiply by 10,000.This takes into consideration the 104 dilution.
62 + 60 = 122
122 / 2 = 61
61 x 10,000 = 610,000 cfu/g
Individual counting technique alone can have a significant effect on the final result depending on how the analyst interprets the growth. Incertain cases it may be difficult to count the colonies; small, obstructed or conjoined colonies may be interpreted differently depending onthe analyst. Spreading colonies in the form of chains should be considered as one colony. In a real life scenario the analyst would be able toconduct a more thorough examination at various angles and light conditions in order to achieve a more accurate count.
Page 121 of 123Issue: 1 09 October 2018
The sample contained Salmonella Nottingham at an approximate level of 11 cfu/375g. The assigned value is therefore Salmonella species‘detected’.
Salmonella Nottingham is characteristic of the genus on selective media and in confirmatory tests, being H2S positive, motile, lactosenegative, citrate positive and urease negative. It is a group I Salmonella according to the Kauffmann-White Scheme, with a full serologicalprofile of ‘O’ antigen: 16; ‘H’ phase 1 antigen: d and ‘H’ phase 2 antigen: e, n, z15.
Analyte: Salmonella species in 375g (P/A)
Sample: 40 - Detection of Salmonella species in skimmed milk powder vial
Round: 269Scheme: Food Microbiology (QMS)
ResultMethodLab ID
DetectedPCRMC0213
DetectedELISAMC0913
DetectedVIDASMC1010
DetectedOtherMC1509
DetectedMKTT/RV + XLD + BGA 37MC1601
DetectedVIDASMC1601
DetectedOtherMC1876
DetectedPCRMC2278
DetectedPCRMC2330
DetectedMKTT/RV + XLD + BGA 37MC2410
DetectedOtherMC2568
DetectedPCRMC2892
DetectedMKTT/RV + XLD + BGA 37MC3000
DetectedOtherMC3016
DetectedPCRMC3172
DetectedChromogenic agarMC3173
DetectedMKTT/RV + XLD + BGA 37MC3228
DetectedOtherMC3228
DetectedVIDASMC3228
Not DetectedPCRMC3574
DetectedMKTT/RV + XLD + BGA 37MC3923
DetectedPCRMC3923
DetectedELISAMC4736
Not DetectedVIDASMC4830
DetectedOtherMC4861
DetectedPCRMC5906
DetectedPCRMC5965
DetectedMKTT/RV + XLD + BGA 37MC6491
DetectedDuPont BAXMC6508
DetectedMKTT/RV + XLD + BGA 37MC6508
DetectedPCRMC6508
DetectedPCRMC6631
DetectedMKTT/RV + XLD + BGA 37MC7277
DetectedMKTT/RV + XLD + BGA 37MC7415
DetectedVIDASMC7591
DetectedELISAMC8305
DetectedOtherMC8305
DetectedMKTT/RV + XLD + BGA 37MC8395
Result
Data Statistics
DetectedAssigned Value
38Number of Results
95%Satisfactory
Methodology Summary
% SatisfactoryMethod
100%MKTT/RV + XLD + BGA 37
80%VIDAS
100%Chromogenic agar
91%PCR
100%ELISA
100%DuPont BAX
100%Other
Comments
Issue: 1 Page 122 of 123 09 October 2018
Analyte: Salmonella species in 375g (P/A)
Sample: 40 - Detection of Salmonella species in skimmed milk powder vial
Round: 269Scheme: Food Microbiology (QMS)
Salmonellae are Gram-negative non-spore forming rods that are oxidase negative and catalase positive, and are members of theEnterobacteriaceae family. Almost all Salmonella serotypes cause acute gastro-enteritis and account for a large proportion of all foodpoisoning outbreaks. Primary sources of Salmonellae are the gastrointestinal tract of animals; the organism can be transmitted via faecesinto soil, water, feeds, foods and other animals, including humans.
Issue: 1 Page 123 of 123 09 October 2018