food quality controlmeat produce grains spices beverages milk pet food ... spp. poultry and raw...
TRANSCRIPT
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Food Quality Control
Objectives:
Food Customer Impact
Food QC Pathogens
Regulations
Why and How QC is Performed
Certified Reference Materials
Microbiologics Products
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Increasing Customer Impact
Blue Bell – 3 deaths - ListeriaChipotle – $2 Billion and counting
Food Product Recalls
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Protecting Consumers
Salmonellosis
• Tens of millions of human cases worldwide every year (WHO)
Listeriosis
• Particularly high case-fatality rate (CDC)
• 1600 illnesses and 260 deaths yearly in the US
Enterohaemoragic E. coli
• May lead to life-threatening disease especially in young children and the elderly
Campylobacter
• Especially problematic for the young, the elderly and the immunocompromised
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Examples of Foods that are Tested
Meat
Produce
Grains
Spices
Beverages
Milk
Pet Food
Animal Feed
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Common Food Pathogens
Pathogens Food Examples
Salmonella spp. Raw eggs, poultry, beef and pork
Campylobacter spp. Poultry and raw dairy products
Listeria monocytogenes Cheeses, raw vegetables, ice cream, and deli meats
Listeria spp. Cheeses, raw vegetables, ice cream, and deli meats
E. coli O157:H7 Raw/undercooked ground beef and sprouts
Non-O157 Shiga-Toxin E. coli (STEC) Raw/undercooked ground beef
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Common Spoilage and Indicator Organisms
Organism (group) Sample Type
Aerobic plate counts (APC)Environmental samples, frozen, chilled, pre-cooked,prepared foods, and product shelf life determination
Coliforms / Generic E. coliEnvironmental samples, processing water, and equipment surfaces
Enterobacteriaceae (EB)Environmental samples, processing water, and equipment surfaces
Yeast and mold Processed foods and raw vegetables
Spores Processed and canned foods
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Food QC Testing Regulations
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Who Says You Need to Perform QC Testing?
The Consumer
Government & Standards
OrganizationsIndustry
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ISO 11133 and WDCM
ISO 11133: Microbiology of food, animal feed and water – Preparation, production, storage and performance testing of culture media
Cites the use of reference strains represented by a WDCM number
ATCC® strains have WDCM numbers; Microbiologics catalog cross-referenced to WDCM
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Food Safety Modernization Act (FSMA) became law on January 4, 2011
Seven rules have been finalized to date, addressing:
Preventive Controls for Human Food
Preventive Controls for Animal Food
Foreign Supplier Verification Program
Accredited Third-Party Certification
Produce Safety
Sanitary Transportation
Intentional Adulteration
· FSMA says:
“… facilities have a responsibility to choose testing laboratories that will produce reliable and accurate test results.” (Preventive Controls for Human Food)
Food Safety Modernization Act
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FSMA says:
“For a regulatory audit, (when) sampling and analysis is conducted, the accredited third-party certification body must use a laboratory accredited in accordance with ISO/IEC 17025:2005 or another laboratory accreditation standard that provides at least a similar level of assurance in the validity and reliability of sampling methodologies, analytical methodologies, and analytical results.” (3rd Party)
· FSMA says:
Importers must “retain documentation of each sampling and testing of a food, including … the test(s) conducted (including the analytical method(s) used)… the results of the testing… and information identifying the laboratory conducting the testing...” (FSVP)
· More rules and guidance documents anticipated, including
Laboratory Accreditation for Analyses of Foods
Model Laboratory Standards
Food Safety Modernization Act
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Regulations Driving CRM’s
Food Safety Modernization Act (FSMA) became law on January 4, 2011. FSMA says:
• “…facilities have a responsibility to choose testing laboratories that will produce reliable and accurate test results.” (Preventive Controls for Human Food)
• “For a regulator audit, (when) sampling and analysis is conducted, the accredited third-party certification body must use a laboratory accredited in accordance with ISO/IEC 17025:2005 or other laboratory accreditation standard that provides at leas a similar level of assurance in the validity and reliability of sampling methodologies , analytical methodologies, and analytical results.” (3rd Party)
• Importers must “retain documentation of each sampling and testing of a food, including the test(s) conducted…the results of the testing…and information identifying the laboratory conducting the testing…” (FSVP)
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Organizations and Standards
Organization/ Standard
What It’s About
AOACPublishes approved methods on their website –Official Methods of Analysis
ISO 17025General requirements for the competence of testing and calibration laboratories
ISO 11133Microbiology of food and animal feeding stuffs – Guidelines on preparation and production of culture media
BAM The FDA’s Bacterial Analytical Module of food and cosmetics
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No matter what regulations you face, quality control in the microbiology lab is
essential…
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Why Are Controls Important?
Numerous factors can lead to inaccurate results…
Unacceptable media
Instrument or equipment malfunction
Defective reagents
Technician error
Interfering components in the food may mask the presence of pathogens
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1. Presence/absence (qualitative) tests for detecting pathogens such as Salmonella, Campylobacter, and Listeria monocytogenes
2. Enumerated (quantitative) tests for determining the number of targeted organisms in a food sample
How is Food QC Testing Performed?
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Presence/Absence
(Qualitative) Testing
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QC for Presence/Absence Testing
QC organisms are used to ensure that the test method is able to detect pathogens, if present
• Example: KWIK-STIK and LYFO DISK
QC can be used to verify that the test method is not only able to detect pathogens, but detect pathogens that are present at very low levels.
• Epower or EZ-FPC
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Presence/Absence Test – 2 Steps
1. Enrichment Step - used to increase low levels of pathogens (that would not otherwise be detected)
• Measure food sample into suitable enrichment broth
• Incubate appropriately to allow bacteria to multiply to detectable levels
2. Detection Step – used to screen the enrichment sample for presence of the pathogen
• Detection steps range from traditional (culture) methods to molecular methods (PCR)
• Some culture methods may take several days, while PCR may only take hours
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Enumerated
(Quantitative) Testing
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Types of Enumerated (Quantitative) Testing
Bacterial and spore enumeration on samples such as:
• Milk, juice
• Carcass swabs and rinses
• Bread
• Canned goods
Indicator and food spoilage organism enumeration on samples such as:
• Carcass swabs and rinses
• Processing water
• Environmental samples
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Quantitative Tests
Range from traditional to rapid
No enrichment step required (the number of bacteria cells that are in the product at that time are counted). Examples of methods:
• Pour Plate/Spread plate
• Film-Based Media (Petrifilm)
• Autoplate® Spiral Plating System
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Example of Film-Based Test for E. coli
1. Prepare the food product as required
2. Inoculate Petrifilm with 1.0 ml food sample or food rinse
3. Incubate appropriately
4. Enumerate CFUs
Petrifilm™ test for E.coli
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Why Perform QC in a Food Lab?
1. Positive and negative controls of each test batch
2. New batches of media
3. Determine if test method is in control (SPC)
4. Validation of new test methods, instrumentation, test kits and verification of first-time test performance
5. Proficiency: test the competency of the laboratory technicians and test methods
Media Testing
Instrumentation
Lab Techs
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1. QC of Each Test Batch
Positive and Negative Controls
Verify that:
• Media and reagents are performing satisfactorily
• Equipment is functioning properly
• Analyst is performing test properly
• Components in sample do not interfere with testing system
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Example Requirements
The USDA FSIS Establishment Guidance for the Selection of a Commercial or Private Microbiological Testing Laboratory; March 2012 states:
“The testing laboratory should have routine control with each batch of samples.”
ISO 17025: General requirements for the competence of testing and calibration laboratories states:
“The laboratory shall have quality control procedures for monitoring the validity of tests.”
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2. QC of New Batches of Media
Media is tested for productivity, selectivity, and specificity.
ISO 11133 has strict requirements for media made in-house
• Enumerated tests on agar uses 80-120 CFU (outside range 50-150 CFU)
• Enrichment broth uses less than 100 CFU
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ISO 11133: Culture Media Guidelines
Who follows it?
• Europe
• Some countries in South America and Asia
• Manufacturers of media
• Labs that prepare their own media for quantitative testing must test all lots
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4. Validation and Verification of New Test Methods
Validate all methods, instrumentation, test kits, and lab techs
Requires testing with numerous target and non-targeted strains
Verification
• New test kits
Food Matrix Extensions
Known QC strains produce known reactions
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What is Certified Reference Material (CRM)?
Manufacturer must be certified to ISO Guide 34, and product names must be listed on a scope document
Must come with a certificate providing traceability and uncertainty.
1. Characterization – Verification of Identity
2. Homogeneity – Variability of values from one pellet to another
3. Transport – Variability of values before and after transport
4. Stability – Variability of values over shelf-life
• Certified Value
• Expanded Uncertainty
• Standard Deviation
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Role of CRM in Food QC Testing and ISO 17025
For Testing Laboratories, Section 5.6.3.2 of ISO 17025:2005 states
“Reference materials shall, where possible, be traceable to SI units of measurement, or to certified reference materials”
Examples CRM use in testing:
Method Validation
Method Verification
Method monitoring or process control
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Microbiologics QC Food Products
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Products for Presence/Absence (Qualitative) Testing
KWIK-STIK
KWIK-STIK Plus
LYFO DISK
Lab-Elite CRM
UV-BioTAG
Epower
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New Product – UV-BioTAG (Qualitative)
UV-BioTAG cultures fluoresce under ultra-violet light to visibly distinguish these QC strains from other contaminates.
• Available in four strains in both vial and swab format
• UV-BioTAG strains are genetically modified microorganisms
• Note: We are working on getting the required approvals for importing these products into the EU due to GMO restrictions.
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Products for Enumerated (Quantitative) Testing
Epower
Epower CRM
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Product Highlight: STECs
Shiga-toxin producing E.coli is banned by the USDA/FSIS in non-intact beef
Microbiologics offers the STECs in a prepared format sourced from the CDC and ATCC®
Prevalent in beef around the world
Found in vegetables (2011 sprouts in Germany O104:H4)FoodQualityNews.com
Microbiologics is the only accredited supplier to offer STEC controls in ready-to-use format
Be compliant with USDA/FSIS regulations
Reduce Risk
Easy-to-use
Saves time, labor and money
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Water: Life’s Link
Objectives:
Water Market Overview
Water Testing
Microbiologics Product Overview
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Our Most Precious Resource
Water costs are on the rise
1 in 7 people have no access to safe drinking water
Ground water depletion
Drought
Increased food demands
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Why Test Water?
People contaminate water (within the first 15 minutes of bathing, the average person sheds 6,000,000 colony forming units (CFU) of S. aureus)
Farm animals contaminate water
Around 700,000 children die every year from diarrhea caused by unsafe water and poor sanitation
CDC http://www.cdc.gov/healthywater/hygiene/fast_facts.html
http://www1.agric.gov.ab.ca/$department/deptdocs.nsf/all/agdex927
Child Health Epidemiology Reference Group (CHERG), 2012 and Unicef Child Mortality Report, 2012
http://www.wateraid.org/what-we-do/the-crisis/statistics
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Laboratories Can Prevent Illness
Laboratories can help keep people healthy by detecting and reporting bacterial contamination in water samples
Accurate and timely results are important
Quality control helps ensure accurate results
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CRM, ISO 17025 and Standard Methods
Many laboratories are ISO 17025 accredited, and will need Certified Reference Materials
Standard Methods, 9020 QC Lab Guidelines, section 9.2 states, “Positive and negative control cultures –Use certified reference cultures.”
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What are Labs Testing For?
Indicator organisms
– They are found in animal and human waste
– They are a warning sign that pathogens may be present
Legionella
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Common Water Pathogens
Vibrio
Legionella
Salmonella
Shigella
Vibrio
Giardia
Cryptosporidium
E.coli O157:H7
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Indicator Organisms
Coliforms
– Gram negative, non-spore forming
E. coli (not O157:H7 or STEC)
– Specific to animals and humans
Enterococci
– Found in animal & human waste
– Can survive in salt water
Clostridium perfringens
– Ubiquitous in fecal matter
– Seen in water and wastewater
– Spores resistant to wastewater treatment
Pseudomonas
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Common Testing Methods
Most common methods:
– Membrane filtration method
– Enzyme substrate method
– MPN/Fermentation Broth Method
• Multiple tubes are used to estimate the coliform and/or E. coli concentration in a water sample
– Heterotrophic Plate Method
• Estimates the number of viable bacteria in a water sample
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Microbiologics Solutions
KWIK-STIK
LYFO DISK
Lab-Elite CRM
Epower, Epower CRM
Growth Promotion Test Products