fraction collector f9-c and f9-r operating instructions

Fraction collectorF9-C and F9-ROperating instructionsOriginal instructions

Table of Contents41 Introduction ..........................................................................................................51.1 About this manual ................................................................................................................................61.2 Important user information .............................................................................................................81.3 Regulatory information ......................................................................................................................91.3.1 EU Directives ....................................................................................................................................

101.3.2 Eurasian Customs Union ............................................................................................................111.3.3 Regulations for USA and Canada ...........................................................................................121.3.4 Other regulations and standards ............................................................................................

132 Safety instructions ...............................................................................................142.1 Safety precautions ...............................................................................................................................212.2 Labels .........................................................................................................................................................242.3 Emergency procedures ......................................................................................................................262.4 Recycling information .........................................................................................................................272.5 Declaration of Hazardous Substances (DoHS) ........................................................................

293 Fraction collector overview ...............................................................................303.1 Fraction collector F9-C ......................................................................................................................313.1.1 Function .............................................................................................................................................323.1.2 Fraction collector F9-C illustrations .......................................................................................363.1.3 Cassettes, Cassette tray and racks ........................................................................................403.1.4 Connect tubing to the ÄKTA pure instrumentFraction collector F9-C ....................413.2 Fraction collector F9-R ......................................................................................................................423.2.1 Function .............................................................................................................................................433.2.2 Fraction collector F9-R illustrations .......................................................................................463.2.3 Tubes ...................................................................................................................................................483.2.4 Connect tubing to ÄKTA pure or ÄKTA avant .....................................................................

494 Installation ............................................................................................................504.1 Room and site requirements ...........................................................................................................514.1.1 Delivery and storage ....................................................................................................................524.1.2 Placement and space requirements ......................................................................................554.1.3 Site requirements ...........................................................................................................................564.2 Connecting two fraction collectors to ÄKTA pure ..................................................................574.2.1 Power and communication .......................................................................................................634.2.2 Connect tubing ................................................................................................................................654.3 Prepare waste tubing for Fraction collector F9-C .................................................................674.4 Set the delay volume ..........................................................................................................................694.5 Performance test ..................................................................................................................................

705 Operation ..............................................................................................................715.1 Prepare Fraction collector F9-C .....................................................................................................785.2 Fraction collector F9-R .......................................................................................................................

2 Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29025606 AC

Table of Contents

856 Maintenance .........................................................................................................866.1 Clean Fraction collector F9-C ..........................................................................................................886.2 Fraction collector F9-R .......................................................................................................................

897 Reference information ........................................................................................

90Index .......................................................................................................................

Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29025606 AC 3

Table of Contents

1 Introduction

About this chapterThis chapter contains important user information, description of safety notices, regula-tory information and a general description of the intended use of Fraction collector F9-Cand Fraction collector F9-R.

In this chapterThis chapter contains the following sections:

See pageSection

51.1 About this manual

61.2 Important user information

81.3 Regulatory information


1 Introduction

1.1 About this manual

Purpose of this manualThe Operating Instructions provide you with the information needed to install, operateand maintain the product in a safe way.

Typographical conventionsSoftware items are identified in the text by bold italic text. A colon separates menu levels,thus File:Open refers to the Open command in the File menu.

Hardware items are identified in the text by bold text (for example, Power).


1 Introduction1.1 About this manual

1.2 Important user information

Read this before operating theproduct

All users must read the entire Operating Instructions before installing, operating ormaintaining the product.

Always keep the Operating Instructions at hand when operating the product.

Do not operate the product in any other way than described in the user documentation.If you do, you may be exposed to hazards that can lead to personal injury and you maycause damage to the equipment.

Intended useFraction collector F9-C and Fraction collector F9-R are automated fraction collectorsintended for the collection of fractions from purification runs. Fraction collector F9-Cand Fraction collector F9-R are intended for research use only, and shall not be used inclinical procedures, or for diagnostic purposes.

PrerequisitesIn order to operate Fraction collector F9-C and Fraction collector F9-R safely and accord-ing to the intended purpose the following prerequisites must be met:

• You should understand the concepts of liquid chromatography.

• You should be familiar with the purification instrument and system and have readthe Operating Instructions.

• You must read and understand the Safety chapter of these Operating Instructions.

• The system must be installed according to the instructions in Chapter 4 Installation,on page 49.


1 Introduction1.2 Important user information

Safety noticesThis user documentation contains safety notices (WARNING, CAUTION, and NOTICE)concerning the safe use of the product. See definitions below.

WARNINGWARNING indicates a hazardous situation which, if not avoided,could result in death or serious injury. It is important not to proceeduntil all stated conditions are met and clearly understood.

CAUTIONCAUTION indicates a hazardous situation which, if not avoided,could result in minor or moderate injury. It is important not to pro-ceed until all stated conditions are met and clearly understood.

NOTICENOTICE indicates instructions that must be followed to avoiddamage to the product or other equipment.

Notes and tipsA note is used to indicate information that is important for trouble-free andoptimal use of the product.

Note:

A tip contains useful information that can improve or optimize your procedures.Tip:


1 Introduction1.2 Important user information

1.3 Regulatory information

IntroductionThis section lists the directives and standards that are fulfilled by Fraction collector F9-Cand Fraction collector F9-R.

Manufacturing informationThe table below summarizes the required manufacturing information.

InformationRequirement

GE Healthcare Bio-Sciences AB,Name and address of manufacturer

Björkgatan 30, SE 751 84 Uppsala, Sweden

In this section

See pageSection

91.3.1 EU Directives

101.3.2 Eurasian Customs Union

111.3.3 Regulations for USA and Canada

121.3.4 Other regulations and standards


1 Introduction1.3 Regulatory information

1.3.1 EU Directives

Conformity with EU DirectivesThis product fulfills the European Directives listed below. See the EU Declaration ofConformity for the directives and regulations that apply for the CE marking.

If not included with the product, a copy of the EU Declaration of Conformity is availableon request.

TitleDirective

Machinery Directive (MD)2006/42/EC

Electromagnetic Compatibility (EMC) Directive2014/30/EU

Low Voltage Directive (LVD)2014/35/EU

Restriction of Hazardous Substances (RoHS) Directive2011/65/EU

CE marking

The CE marking and the corresponding EU Declaration of Conformity is valid for the in-strument when it is:

• used according to the Operating Instructions or user manuals, and

• used in the same state as it was delivered from GE, except for alterations describedin the Operating Instructions or user manuals.



1.3.1 EU Directives

1.3.2 Eurasian Customs Union

IntroductionThis section contains additional regulatory information to comply with the EurasianCustoms Union technical regulations.

Manufacturer and importerinformation

The table below summarizes the manufacturer and importer information required bythe Eurasian Customs Union.

InformationRequirement

See Manufacturing informationName and address of manufacturer

Telephone: + 46 771 400 600Telephone number of manufacturer

GE Healthcare LLCImporter and/or company for obtain-ing information about importer GE Healthcare Life Sciences

Presnenskaya nab., 10C, 12th floor

RU-123 317 Moscow, Russian Federation

Telephone 1: + 7 495 411 9714

Fax nr: + 7 495 739 6932

Email: [email protected]


1 Introduction1.3 Regulatory information1.3.2 Eurasian Customs Union


FCC complianceThis device complies with part 15 of the FCC Rules. Operation is subject to the followingtwo conditions: (1) This device may not cause harmful interference, and (2) this devicemust accept any interference received, including interference that may cause undesiredoperation.

The user is cautioned that any changes ormodifications not expressly approvedby GE could void the user’s authority to operate the equipment.

Note:

This equipment has been tested and found to comply with the limits for a Class A digitaldevice, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-sonable protection against harmful interference when the equipment is operated in acommercial environment. This equipment generates, uses, and can radiate radio frequen-cy energy and, if not installed and used in accordance with the instruction manual, maycause harmful interference to radio communications. Operation of this equipment in aresidential area is likely to cause harmful interference in which case the user will be re-quired to correct the interference at his own expense.

CAN ICES/NMB complianceThis product complies with the Canadian standard ICES-001/NMB-001 concerningelectromagnetic compatibility.




1.3.4 Other regulations and standards

IntroductionThis section describes the standards that apply to Fraction collector F9-C and Fractioncollector F9-R.

Environmental conformityThis product conforms to the following environmental requirements.

TitleRequirement

Management Methods for the Restriction of the Use of Haz-ardous Substances in Electrical and Electronic Products.

China RoHS

Standards, machinery andelectrical equipment

Standard requirements fulfilled by this product are summarized in the table below.

DescriptionStandard

Safety of machinery. General principles for design. Riskassessment and risk reduction.

EN ISO 12100

Safety requirements for electrical equipment for mea-surement, control, and laboratory use - Part 1: Generalrequirements.

EN 61010-1, IEC 61010-1,UL 61010-1, CAN/CSA-C22.2 No. 61010-1

Electrical Equipment for Measurement, Control, andLaboratory Use-EMC requirements-Part 1: General re-quirements

EN 61326-1

Emission according to CISPR 11, Group 1, class A 1

Industrial, Scientific and Medical (ISM) Radio FrequencyGenerators (Canada)

ICES-001/NMB-001

1

NOTICEThis equipment is not intended for use in residential environ-ments and may not provide adequate protection to radio re-ception in such environments.


1 Introduction1.3 Regulatory information1.3.4 Other regulations and standards

2 Safety instructions

About this chapterThis chapter describes safety precautions and emergency shutdown procedures forFraction collector F9-C and Fraction collector F9-R. The labels on the fraction collectorsand information regarding recycling are also described.


See pageSection

142.1 Safety precautions

212.2 Labels

242.3 Emergency procedures

262.4 Recycling information

272.5 Declaration of Hazardous Substances (DoHS)


2 Safety instructions

2.1 Safety precautions

About this sectionThe safety precautions in this section are grouped in the following categories:

• General precautions, on page 14

• Flammable liquids, on page 15

• Personal protection, on page 16

• Installing and moving the fraction collector, on page 17

• Operation, on page 17

• Maintenance, on page 18

Unless stated otherwise, the precautions apply to both Fraction collector F9-C andFraction collector F9-R.

General precautions

WARNINGDo not operate Fraction collector F9-C and Fraction collector F9-Rin any other way than described in the Operating Instructions.

WARNINGOperation and user maintenance of Fraction collector F9-C andFraction collector F9-R should be performed by properly trainedpersonnel only.

WARNINGDo not use any accessories not supplied or recommended by GE.


2 Safety instructions2.1 Safety precautions

WARNINGDo not use Fraction collector F9-C and Fraction collector F9-R if itis not working properly, nor if it has suffered any damage, for ex-ample:

• damage to the power cord or its plug

• damage caused by dropping the equipment

• damage caused by splashing liquid onto it

Flammable liquids

CAUTIONExplosion hazard during fractionation of flammable liquids. Donot use a covered fraction collector (Fraction collector F9-C) tofractionate flammable liquids. When using an open fraction collec-tor (Fraction collector F9-R), do not place the fraction collector ina closed compartment and make sure that the room ventilationmeets the local requirements.

Fraction collector F9-C

CAUTIONFire Hazard. Do not fractionate flammable liquids using Fractioncollector F9-C. When running RPC methods, or other proceduresusing organic solvents, collect fractions through the outlet valveor Fraction collector F9-R.

CAUTIONExplosion hazard if flammable liquid leaks during cleaning ofthe flow path. When cleaning the flow path of Fraction collectorF9-C with a flammable liquid like ethanol, carefully inspect the flowpath, including the waste tubing, to make sure there will be noleakage.



Fraction collector F9-R

CAUTIONFractionation with flammable liquids using Fraction collectorF9-R. Before you start the run, make sure that the tubes in thefraction collector are of correct size according to fraction size set-tings.

CAUTIONMoving tubes with flammable liquids. Move tubes filled withflammable liquids carefully, to prevent spillages that may come incontact with an ignition source.

Personal protection

CAUTIONAlways use appropriate personal protective equipment during op-eration and maintenance of Fraction collector F9-C and Fractioncollector F9-R.

CAUTIONHazardous substances.When using hazardous chemical and bio-logical agents, take all suitable protective measures, such aswearing protective glasses and gloves resistant to the substancesused. Follow local and/or national regulations for safe operation,maintenance and decommissioning of the equipment.



Installing and moving thefraction collector

WARNINGUniNet cable. Only use UniNet cables delivered or approved by GE.

CAUTIONHeavy object. Use proper lifting equipment, or use two or morepersons when moving Fraction collector F9-C. All lifting and movingmust be performed in accordance with local regulations.

NOTICEVents on Fraction collector F9-C. To ensure adequate ventilation,keep papers and other objects away from the vents of the fractioncollector.

Operation

CAUTIONRisk of breaking test vials. Do not use excessive force to pressvials with incorrect dimensions into the fraction collector. Glassvials may break and cause injuries.

CAUTIONAvoid spillage and overflow. Make sure that the equipment isprepared according to the settings in the method to be run.



Fraction collector F9-C

WARNINGMoving parts. Do not open the door of Fraction collector F9-Cwhile the fraction collector is active. If you need to access thefraction collector, press Pause on the chromatography instrument,and make sure that no parts are moving before opening the door.

CAUTIONMaximum weight on the top of Fraction collector F9-C. Do notplace more than 30 kg on top of Fraction collector F9-C.

Fraction collector F9-R

CAUTIONReplacement of the fraction collector bowl in Fraction collectorF9-R. Do not replace the bowl during a run.

CAUTIONReplacing tubes. Do not replace the tubes in the fraction collectorduring a run.

Maintenance

CAUTIONElectrical shock hazard. All repairs should be done by servicepersonnel authorized by GE Healthcare. Do not open any coversor replace parts unless specifically stated in the user documenta-tion.



CAUTIONDisconnect power. Always switch off power to Fraction collectorF9-C and Fraction collector F9-R, before cleaning any of its compo-nents, unless stated otherwise in the user documentation. This isdone by switching off the chromatographic instrument that pro-vides Fraction collector F9-C and Fraction collector F9-R withpower.

CAUTIONHazardous chemicals andbiological agents.Before maintenance,service and decommissioning, wash Fraction collector F9-C andFraction collector F9-R with a neutral solution to make sure thatany hazardous solvents and biological agents have been flushedout from the fraction collector.

CAUTIONAlways use appropriate personal protective equipment when de-commissioning the equipment.

CAUTIONCleaning Fraction collector F9-C and Fraction collector F9-Rbefore decommissioning.

• Wipe Fraction collector F9-C and Fraction collector F9-R witha damp tissue using a cleaning agent so that no hazardoussolvents or biological agents remain on the surface. Do notforget to clean the interior of Fraction collector F9-C.

• Perform a system CIP using a neutral solution. Make sure thatany hazardous solvents or biological agents are flushed outfrom the fraction collector.



NOTICECleaning. Keep Fraction collector F9-C and Fraction collector F9-Rdry and clean. Wipe regularly with a soft damp tissue and, if neces-sary, a mild cleaning agent. Let Fraction collector F9-C and Fractioncollector F9-R dry completely before use.




2.2 Labels

About this sectionThis section describes the safety labels that are attached to Fraction collector F9-C andFraction collector F9-R.

Labels on Fraction collector F9-CThe label below is located both on the back of the fraction collector and on the top wallof its interior.

Fraction collector F9-Csafety labelIt is not allowed to fractionate flammable liquids using Fraction collector F9-C due tothe risk of build-up of flammable and explosive gases. When running RPC methods, orother procedures using organic solvents, collect fractions through the Outlet valve orwith Fraction collector F9-R.

The safety label below is located on the inside of the door of Fraction collector F9-C.

Do NOT fractionateflammable liquids.


2 Safety instructions2.2 Labels

Labels on Fraction collector F9-RThe label is located on the back of the fraction collector.

Label informationThe label contains information on the serial number, regulatory compliance symbols,recycling symbols, and specifications.

The label information is explained in the table below.

MeaningLabel text

Fraction collector code numberCode no

Fraction collector serial numberSerial no

Manufacturing year and monthMfg Year

Supply voltageVoltage

Max power consumptionMax Power

Protection class, ingress protection according to IEC60529

Protection class

Warning! Read the Operating Instruction before usingthe module. Do not open any covers or replace partsunless specifically stated in theOperating Instructions.



MeaningLabel text

This symbol indicates that electrical and electronicequipment must not be disposed of as unsorted mu-nicipal waste and must be collected separately. Pleasecontact an authorized representative of the manufac-turer for information concerning the decommissioningof equipment.

This symbol indicates that the product might containhazardous materials in excess of the limits establishedby the Chinese standardSJ/T11363-2006Requirementsfor Concentration Limits for Certain Hazardous Sub-stances in Electronic Information Products.

The fraction collector complies with applicable Euro-pean directives

The equipment complies with the applicable require-ments for Australia and New Zealand

Eurasian Conformity mark: the single conformity markindicates that the product is approved for circulationon the markets of the member states of the EurasianCustoms Union.

CAN ICES-1/NMB-1 indicates that this product complieswith the Canadian standard ICES-001 concerningtechnical requirements relative to radiated noiseemissions from Industrial, Scientific and Medical radiofrequency generators.

CAN ICES-1/NMB-1



2.3 Emergency procedures

About this sectionThe ÄKTA™ instrument supplies the Fraction collector F9-C and Fraction collector F9-Rwith power. This section describes how to perform an emergency shutdown of theFraction collector F9-C and Fraction collector F9-R by shutting down the ÄKTA instrument.This section also describes the results in the event of power failure or network interruption.

Emergency shutdownIn an emergency situation, stop the run by either pausing the run or switching off theinstrument as described below:

then...If you wantto...

• press the Pause button on the Instrument control panel:

Note:

The buttons on the Instrument control panel may be locked. Thisis an option available in the System settings.

or

• click the Pause icon in UNICORN™:

Result: All pumps in the instrument are stopped.

pause the run


2 Safety instructions2.3 Emergency procedures

then...If you wantto...

• press the Power switch to the 0 position, or

• disconnect the power cord from the wall socket.

Result: The run is interrupted immediately.

Note:

The sample and data may be lost as a result of switching off thepower.

switch off theinstrument

Power failureIn case of a power failure or if communication is lost, the run is interrupted immediatelyand all moving parts in the fraction collector are stopped.


2 Safety instructions2.3 Emergency procedures

2.4 Recycling information

IntroductionThis section contains information about the decommisioning of Fraction collector F9-Cand Fraction collector F9-R.

DecontaminationThe product must be decontaminated before decommissioning. All local regulationsmust be followed with regard to scrapping of the equipment.

Disposal of the productWhen taking the product out of service, the different materials must be separated andrecycled according to national and local environmental regulations.

CAUTIONAlways use appropriate personal protective equipment when de-commissioning the equipment.

Disposal of electricalcomponents

Waste comprising electrical and electronic equipment must not be disposed of as un-sorted municipal waste and must be collected separately. Please contact an authorizedrepresentative of the manufacturer for information concerning the decommissioning ofequipment.


2 Safety instructions2.4 Recycling information

2.5 Declaration of Hazardous Substances (DoHS)

根据SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的信息。The following product pollution control information is provided according to SJ/T11364-2014 Markingfor Restriction of Hazardous Substances caused by electrical and electronic products.

电子信息产品污染控制标志说明Explanation of Pollution Control Label

该标志表明本产品含有超过中国标准GB/T 26572 《电子电气产品中限用物质的限量要求》中限量的有害物质。标志中的数字为本产品的环保使用期，表明本产品在正常使用的条件下，有毒有害物质不会发生外泄或突变，用户使用本产品不会对环境造成严重污染或对其人身、财产造成严重损害的期限。单位为年。为保证所申明的环保使用期限，应按产品手册中所规定的环境条件和方法进行正常使用，并严格遵守产品维修手册中规定的定期维修和保养要求。产品中的消耗件和某些零部件可能有其单独的环保使用期限标志，并且其环保使用期限有可能比整个产品本身的环保使用期限短。应到期按产品维修程序更换那些消耗件和零部件，以保证所申明的整个产品的环保使用期限。本产品在使用寿命结束时不可作为普通生活垃圾处理，应被单独收集妥善处理。This symbol indicates the product contains hazardous materials in excess of the limitsestablished by the Chinese standard GB/T 26572 Requirements of concentrationlimits for certain restricted substances in electrical and electronic products. Thenumber in the symbol is the Environment-friendly Use Period (EFUP), which indicatesthe period during which the hazardous substances contained in electrical and elec-tronic products will not leak or mutate under normal operating conditions so that theuse of such electrical and electronic products will not result in any severe environmen-tal pollution, any bodily injury or damage to any assets. The unit of the period is “Year”.

In order to maintain the declared EFUP, the product shall be operated normally ac-cording to the instructions and environmental conditions as defined in the productmanual, and periodic maintenance schedules specified in Product Maintenance Pro-cedures shall be followed strictly.

Consumables or certain parts may have their own label with an EFUP value less thanthe product. Periodic replacement of those consumables or parts to maintain thedeclared EFUP shall be done in accordance with the Product Maintenance Procedures.

This product must not be disposed of as unsorted municipal waste, and must becollected separately and handled properly after decommissioning.


2 Safety instructions2.5 Declaration of Hazardous Substances (DoHS)

有害物质的名称及含量Name and Concentration of Hazardous Substances

产品中有害物质的名称及含量Table of Hazardous Substances’ Name and Concentration

有害物质Hazardous substance

部件名称Component name

多溴二苯醚PBDE

多溴联苯PBB

六价铬(Cr(VI))

镉Cd

汞Hg

铅Pb

0

0

0

0

0

0

0

0

0

0

X

X

29027743

29011362

本表格依据SJ/T 11364的规定编制。This table is prepared according to SJ/T 11364.

0: 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572规定的限量要求以下。

X: 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572规定的限量要求。

• 此表所列数据为发布时所能获得的最佳信息.

0: Indicates that this hazardous substance contained in all of the homogeneous materials forthis part is below the limit requirement in GB/T 26572.

X: Indicates that this hazardous substance contained in at least one of the homogeneousmaterials used for this part is above the limit requirement in GB/T 26572

• Data listed in the table represents best information available at the time of publication.


2 Safety instructions2.5 Declaration of Hazardous Substances (DoHS)

3 Fraction collector overview

About this chapterThis chapter gives overviews of Fraction collector F9-C and Fraction collector F9-R.


See pageSection

303.1 Fraction collector F9-C

413.2 Fraction collector F9-R


3 Fraction collector overview

3.1 Fraction collector F9-C

About this sectionThis section shows an overview of Fraction collector F9-C.

Technical details are found in the ÄKTA pure User manual.

In this sectionThis section contains the following subsections:

See pageSection

313.1.1 Function

323.1.2 Fraction collector F9-C illustrations

363.1.3 Cassettes, Cassette tray and racks

403.1.4 Connect tubing to the ÄKTA pure instrumentFraction collectorF9-C


3 Fraction collector overview3.1 Fraction collector F9-C

3.1.1 FunctionThe fraction collector collects fractions from ÄKTA pure purification runs.

The fraction collector can be used for:

• Fixed volume fractionation

• Peak fractionation

• Combined fixed volume fractionation and peak fractionation

Fraction collector F9-C has the following functions for reducing sample spill duringfractionation:

• Drop Sync

• Accumulator



3.1.1 Function


IntroductionThis section provides illustrations of Fraction collector F9-C. The main features andcomponents are indicated.

Front viewThe illustration below shows the main parts of the exterior of Fraction collector F9-C.

1 2 3 5 64

DescriptionPart

Fractionation indicator1

Symbol indicating that fractionation is ongoing. Do not open the door whilethe indicator is lit.

Door2

Window3

Door handle4

Tubing connector for outlet valve tubing5


3 Fraction collector overview3.1 Fraction collector F9-C3.1.2 Fraction collector F9-C illustrations

DescriptionPart

Vents6

Rear viewThe illustration below shows the rear view of Fraction collector F9-C.

1

1

23

DescriptionPart

Vents1

UniNet-9 D-type connector (for communication and power supply)2

Waste tube3




InteriorThe illustration below shows the main parts of the interior of Fraction collector F9-C.

1

2

3 4 5 6 7 8

9

10

11

12

1314

DescriptionPart

Fractionation arm guide rail1

Fractionation arm main rail2

Lamp3

Tubing guide4

Tubing connection5

Dispenser head6

Fractionation arm7

Tubing guide8

Height exclusion bar9

Waste funnel10

Waste tube11

Tray catch12


3 Fraction collector overview3.1 Fraction collector F9-C3.1.2 Fraction collector F9-C illustrations

DescriptionPart

Waste groove, in case of overflow13

Tray guides14

Dispenser headThe illustration below shows the Dispenser head of Fraction collector F9-C.

1 2 3

4

56

DescriptionPart

Dispenser head1

Nozzle2

Dispenser head cover3

Accumulator (back part of Dispenser head)4

Drop sync sensor5

Type code reader6





IntroductionFractions can be collected in deep well plates and in tubes of different sizes. A numberof cassettes, trays and racks for different tubes and deep well plates are available. Thecassettes are placed on a tray with six cassette positions.

The cassette type codes, tray type code and rack type codes are scanned by the typecode reader to determine the type of cassettes, trays and racks used. For more informa-tion on requirements that need to be fulfilled by the tubes and deep well plates to beused in Fraction collector F9-C, see the ÄKTA pure User manual.

Available cassettes, trays andracks

The following Cassettes and racks are available:

• Cassette 3 ml tubes (for 40 tubes)





• Cassette for deep well plate (24, 48, 96 wells)

• Cassette tray (for six cassettes)

• Rack for 50 ml tubes (for 55 tubes)

• Rack for 250 ml bottles (for 18 bottles)

For information on dimension requirements for tubes and deep well plates to be usedin the fraction collector, see Fraction collector tubes and bottles, on page 38 and Deepwell plates, on page 38 respectively.

Illustrations of Fraction collectorF9-C tray and racks

The illustrations below show the Cassette tray, the Rack for 50 ml tubes and the Rackfor 250 ml bottles.

The fronts of the tray and the racks are marked with the GE-logotype.

In the Cassette tray, the cassette positions are marked 1 to 6.


3 Fraction collector overview3.1 Fraction collector F9-C3.1.3 Cassettes, Cassette tray and racks

Rack for 250 ml bottlesRack for 50 ml tubesCassette tray

The tray and racks are inserted into the fraction collectorwith theGE-logotypefacing outwards.

Note:

Do not use the Cassette tray when a rack for tubes or bottles is placed in thefraction collector.

Note:

Illustration of Cassettes on theCassette tray

The illustration below shows Cassettes placed on the Cassette tray.

DescriptionPart

Cassette tray1

Cassette position number2

Cassette3

Cassette type code4

Tubes or deep well plates placed in a Cassette5




Fraction collector tubes andbottles

The tubes and bottles used in Fraction collector F9-C must fulfill the requirements listedin the table below. Examples of manufacturers are also listed in the table.

Examples ofmanu-facturers

Height (mm)Diameter (mm)Tubeorbottlesize (ml)

Max.Min.Max.Min.

NUNC™565011.510.53

VWR™76701210.55

BD™ Biosciences,VWR

1029613.3128

BD Biosciences120114171615

BD Biosciences116110302850

Nalgene™, Kautex™121-L: 64.5W: 641

L: 55W: 55 1

250 ml bottle

1 Length and width of the rectangular bottle base

Deep well platesRequirementsThe deep well plates used in Fraction collector F9-C must fulfill the requirements listedin the table below.

SpecificationProperty

24, 48, or 96No. of wells

Square, not cylindricalShape of wells

10, 5, or 2 mlWell volume

Approved deep well platesThe plates listed in the table below are tested and approved by GE to be used withFraction collector F9-C.


3 Fraction collector overview3.1 Fraction collector F9-C3.1.3 Cassettes, Cassette tray and racks

Part no.ManufacturerPlate type

7701-5200 (Whatman™)GE96 deep well plate

353966BD Biosciences

780270Greiner Bio-One

219009Porvair Sciences

S30009Seahorse Bioscience™

951033405/ 0030 501.306Eppendorf™

7701-5500 (Whatman)GE48 deep well plate

S30004Seahorse Bioscience

7701-5102 (Whatman)GE24 deep well plate

S30024Seahorse Bioscience




3.1.4 Connect tubing to the ÄKTA pure instrument

3.1.4 Fraction collector F9-CFraction collector F9-C is delivered with all internal tubing in place. The tubing betweenthe fraction collector and purification instrument need to be installed.

Follow the instructions in the table below to connect the tubing from the ÄKTA pure in-strument to the fraction collector.

ActionStep

Connect the tubing Frac to the Frac port on the outlet valve on the ÄKTApure instrument.

1

Connect the other end of the Frac tubing to the inlet port on the fractioncollector.

2

Adjust the delay volume setting in UNICORN, see Section 4.4 Set the delayvolume, on page 67 for more details.

3


3 Fraction collector overview3.1 Fraction collector F9-C3.1.4 Connect tubing to the ÄKTA pure instrumentFraction collector F9-C


About this sectionThis section shows an overview of Fraction collector F9-R.

Technical details are found in the ÄKTA pure User manual and ÄKTA avant User manual.

In this sectionThis section contains the following subsections:

See pageSection

423.2.1 Function

433.2.2 Fraction collector F9-R illustrations

463.2.3 Tubes

483.2.4 Connect tubing to ÄKTA pure or ÄKTA avant


3 Fraction collector overview3.2 Fraction collector F9-R

3.2.1 FunctionThe fraction collector collects fractions from purification runs on ÄKTA pure and ÄKTAavant instruments.

The fraction collector can be used for:

• Fixed volume fractionation

• Peak fractionation

• Combined fixed volume fractionation and peak fractionation

• Collecting fractions in reversed phase separations using organic solvents

Fraction collector F9-R has the following function for reducing sample spill during frac-tionation:

• Drop Sync


3 Fraction collector overview3.2 Fraction collector F9-R3.2.1 Function


IntroductionThis subsection provides illustrations of Fraction collector F9-R. The main features andcomponents are indicated.




Front viewThe illustration below shows the main parts of the Fraction collector.

1

3 4

5

7

6

8

2

FunctionPart

Lock knob1

Stationary part of delivery arm2

Delivery arm3

Tubing connector4

Tube sensor5

Collection tubes6

Tube rack7

Base unit8


3 Fraction collector overview3.2 Fraction collector F9-R3.2.2 Fraction collector F9-R illustrations

Connector panelThe illustration below shows the main parts of the connector panel on the fraction col-lector.

1 22

FunctionPart

Node ID switch1

UniNet-9 F-type connector (for communication and power supply)2




3.2.3 Tubes

IntroductionFor Fraction collector F9-R the fractions are collected in tubes of different sizes. For moreinformation on requirements that need to be fulfilled by the tubes to be used in Fractioncollector F9-R, see the ÄKTA pure User manual or the ÄKTA avant User manual.

Available tubesTubes with the following diameter can be used with Fraction collector F9-R:

• 12 mm

• 18 mm

• 30 mm

The tubes can have a tube length between 50-180 mm.


3 Fraction collector overview3.2 Fraction collector F9-R3.2.3 Tubes

Illustration of the Fractioncollector F9-R Tube rack

Each tube rack is made up of a combination of a Bowl, Tube support, Tube guide andTube holder. For more information on the assembly of the tube rack, see Assemble theTube rack, on page 79. For information on which Tube rack to use, see the ÄKTA pureUser manual or the ÄKTA avant User manual.

1

2

3456

FunctionPart

Single cutout1

L-shaped cutout2

Bowl3

Tube support4

Tube guide5

Tube holder6

Note that the tube guide has both single and L-shaped cutouts, while the tubeholder only has single cutouts. See Prepare the fraction collector, on page 78for more information.

Note:



3.2.3 Tubes

3.2.4 Connect tubing to ÄKTA pure or ÄKTA avant

Follow the instructions in the table below to connect the tubing from the fraction collectorto ÄKTA pure. For connecting the tubing to ÄKTA avant, see the ÄKTA avant Usermanual.

ActionStep

Lift out the Tubing holder (4) from the Delivery arm (1).1

3

4

5

6

Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut(5) from the Tubing holder.

2

Insert the tubing through the Tubing holder.3

Place the Tubing holder with the tubing over the Tube adjustment cavity (2)of the Delivery arm. Push the tubing down against the bottom of the Tubeadjustment cavity, and then fingertighten the Tubing holder nut. This ensuresthe correct length of the exposed tubing end (6).

4

Re-install the tubing holder in the Delivery arm.5

For Fraction collector F9-R: Connect the tubing from the fraction collectorto the port Frac on the outlet valve.

6

For Fraction collector F9-R, 2nd: Connect the tubing from the second fractioncollector to:

• port Out 10 on Outlet valve V9-O or V9H-O

• port Out 1 on Outlet valve V9-Os or V9H-Os

Adjust the delay volume setting in UNICORN to the volume of the tubing, seeSection 4.4 Set the delay volume, on page 67 for more details.

7


3 Fraction collector overview3.2 Fraction collector F9-R3.2.4 Connect tubing to ÄKTA pure or ÄKTA avant

4 Installation

About this chapterThis chapter provides required information to enable users and service personnel to install,move and transport Fraction collector F9-C and Fraction collector F9-R.

In this chapter

See pageSection

504.1 Room and site requirements

564.2 Connecting two fraction collectors to ÄKTA pure

654.3 Prepare waste tubing for Fraction collector F9-C

674.4 Set the delay volume

694.5 Performance test


4 Installation

4.1 Room and site requirements

About this sectionThis section describes the site planning and the preparations necessary for the installationof Fraction collector F9-C and Fraction collector F9-R. The purpose is to provide plannersand technical staff with the data needed to prepare the laboratory for the installation.

In this section

See pageSection

514.1.1 Delivery and storage

524.1.2 Placement and space requirements

554.1.3 Site requirements


4 Installation4.1 Room and site requirements


About this sectionThis section describes the requirements for receiving the delivery box and storing Fractioncollector F9-C and Fraction collector F9-R before installation.

When you receive the delivery• Record on the receiving documents if there is any apparent damage on the delivery

box. Inform your GE representative of such damage.

• Move the delivery box to a protected location indoors.

Storage requirementsThe delivery box should be stored in a protected place indoors. The following storagerequirements must be fulfilled for the unopened box:

Allowed rangeParameter

-25°C to 60°CAmbient temperature, storage

up to 90% atmospheric humidity at 40°C for 48hrs

Relative humidity





About this sectionThis section describes different options of where to place Fraction collector F9-C andFraction collector F9-R and the space required.

LocationPlace the fraction collector on a clean, flat and stable area that is able to support theweight of the fraction collector.

The following locations are recommended for the Fraction collector F9-C and the Fractioncollector F9-R:

• on the bench to the left of the purification instrument

• on a shelf below the bench

Longer tubing length increases back pressure and band broadening in thechromatographic process. Place the fraction collector so that the total tubinglength is minimized.

Note:

Space requirementsThe illustration below shows the space recommended for the system including Fractioncollector F9-C when used with ÄKTA pure.

39 cm 60 cm

63 cm

54 cm Min 10 cm 80 cm


4 Installation4.1 Room and site requirements4.1.2 Placement and space requirements

The illustration below shows the space recommended for the system including Fractioncollector F9-R when used with ÄKTA pure.

63 cm

80 cmMin 10 cm54 cm32 cm

60 cm

Fraction collector F9-R requires 10 cm free space above the fractionation armfor handling of tubes.

Note:

Fraction collector weight

WeightItem

21 kgFraction collector F9-C

5 kgFraction collector F9-R




Fraction collector dimensionsThe outer dimensions of Fraction collector F9-C are shown in the illustration below.

585 mm

390 mm

320 mm

The outer dimensions of Fraction collector F9-R are shown in the illustration below.

400 mm

320 mm

250 mm


4 Installation4.1 Room and site requirements4.1.2 Placement and space requirements


About this sectionThis section describes the site requirements for installation of Fraction collector F9-Cand Fraction collector F9-R.




4.2 Connecting two fraction collectors to ÄKTA pure

About this sectionThis section contains information on how to set up tubing and a connection for powerand communication between Fraction collector F9-C or Fraction collector F9-R and theÄKTA pure instrument.

This information can apply to other ÄKTA purification instruments. Pleasecheck the purification instrument documentation for more information.

Note:

It is possible to install up to two fraction collectors in the following combinations whenusing ÄKTA pure:

• one Fraction collector F9-C and one Fraction collector F9-R, or

• two Fraction collector F9-R.

If two fraction collectors are to be used, the following applies to the second one:

• it must be a Fraction collector F9-R,

• it has to be configured as Fraction collector F9-R, 2nd,

• the node ID has to be changed, as described in Check/change Node ID, on page 61.

In this section

See pageSection

574.2.1 Power and communication

634.2.2 Connect tubing


4 Installation4.2 Connecting two fraction collectors to ÄKTA pure


IntroductionThis subsection describes how to set up the power and communication connection be-tween Fraction collector F9-C or Fraction collector F9-R and the ÄKTA pure instrument.

Connect fraction collectorThe following cables are used to connect the fraction collectors to the ÄKTA pure instru-ment. Use the specified cable and follow the instructions below.

CableFraction collector

UniNet-9 cable, D-typeFraction collector F9-C

UniNet-9 cable, F-typeFraction collector F9-R

WARNINGUniNet cable. Only use UniNet cables delivered or approved by GE.




ActionStep

Switch off the power to the ÄKTA pure instrument.1

Remove the jumper from the UniNet-9 port to be used on the rear of theÄKTA pure instrument (port 7 or 8 for Fraction collector F9-C, port 1 to 6 forFraction collector F9-R).

2


4 Installation4.2 Connecting two fraction collectors to ÄKTA pure4.2.1 Power and communication

ActionStep

Connect the UniNet-9 cable between the UniNet-9 ports on the back of thefraction collector and on the back of the ÄKTA pure instrument.

3

NOTICEDo not use the Test port on the ÄKTA pure instrument.

2

Fraction collector F9-RFraction collector F9-C

Make sure that all unused UniNet-9 ports on the ÄKTA pure instrument areplugged with jumpers.

4

Software configurationWhen the fraction collector has been installed, the System properties for the systemhas to be updated in UNICORN. The system will restart automatically when the configu-ration has been changed and the system can be reconnected.

From hereon, UNICORN refers to UNICORN 6.3 or other compatible versions of the soft-ware. The examples given in these Operating Instructions refer to UNICORN 6.3.

Follow the instructions below to update the system in UNICORN.




ActionStep

• In the Administration module, choose Tools:System Properties or clickthe System Properties icon to open the dialog.

Result: The System Properties dialog is displayed.

• Select a system in the System Properties dialog.

Note:

Only active systems can be edited.

• Click the Edit button.

Result: The Edit dialog is displayed.

1



ActionStep

In the Edit dialog:

• Select Fraction collectors from the Component types list.

Result: All available fraction collectors are shown in the Component se-lection list.

Note:

Instrument modules are referred to as Components in UNICORN.

Note:

An outlet valve has to be selected in order to select a fraction collector.

• Select the Fraction collector check box.

• Choose what kind of fraction collector to add from the drop down listbox.

• Note:

Fraction collector 2 is only available in the Component selection list ifFraction collector is already selected.

2

Click the OK button to apply the changes.3

Check/change Node IDThe Node ID for Fraction collector F9-C cannot be changed.

The Node ID for Fraction collector F9-R needs to be changed if

• the fraction collector will be used as secondary fraction collector, fraction collector,2nd.




• the fraction collector is to be used as primary fraction collector after it has been usedas secondary.

The Node ID for Fraction collector F9-R is set by positioning the arrow of the rotatingswitch at the back of the Fraction collector. Use a screwdriver to set the arrow of theswitch to the desired number.

Node IDOrderFraction collector

01stFraction collector F9-C

01stFraction collector F9-R

12nd




IntroductionThis subsection contains information on how to connect tubing from ÄKTA pure toFraction collector F9-C and Fraction collector F9-R.

Fraction collector F9-CFraction collector F9-C is delivered with all internal tubing in place. The tubing betweenthe fraction collector and purification instrument need to be installed.

Follow the instructions in the table below to connect the tubing from the ÄKTA pure in-strument to the fraction collector.

ActionStep

Connect the tubing Frac to the Frac port on the outlet valve on the ÄKTApure instrument.

1

Connect the other end of the Frac tubing to the inlet port on the fractioncollector.

2

Adjust the delay volume setting in UNICORN, see Section 4.4 Set the delayvolume, on page 67 for more details.

3




Fraction collector F9-RFollow the instructions in the table below to connect the tubing from the fraction collectorto the ÄKTA pure instrument.

ActionStep

Lift out the Tubing holder (4) from the Delivery arm (1).1

3

4

5

6

Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut(5) from the Tubing holder.

2

Insert the tubing through the Tubing holder.3

Place the Tubing holder with the tubing over the Tube adjustment cavity (2)of the Delivery arm. Push the tubing down against the bottom of the Tubeadjustment cavity, and then fingertighten the Tubing holder nut. This ensuresthe correct length of the exposed tubing end (6).

4

Re-install the tubing holder in the Delivery arm.5

For Fraction collector F9-R: Connect the tubing from the fraction collectorto the port Frac on the outlet valve.

6

For Fraction collector F9-R, 2nd: Connect the tubing from the second fractioncollector to:

• port Out 10 on Outlet valve V9-O

• port Out 1 on Outlet valve V9-Os

Adjust the delay volume setting in UNICORN to the volume of the tubing, see, on page 67 for more details.

7


4 Installation4.2 Connecting two fraction collectors to ÄKTA pure4.2.2 Connect tubing

4.3 Prepare waste tubing for Fraction collector F9-CThe waste outlet is located on the rear of the fraction collector. See instructions belowfor how to prepare the waste tubing.

InstructionsFollow the instructions below to prepare the waste tubing.

ActionStep

Insert the waste tubing in a vessel.1

NOTICEThe maximum level of the vessel for the waste tubingmust be lower than the fraction collector.


4 Installation4.3 Prepare waste tubing for Fraction collector F9-C

ActionStep

Cut the waste tubing to appropriate length. It is important that the tubingis not bent and will not be submerged in liquid during the run.

2

Note:

If the tubing is too short, replace it with new tubing. Do not lengthen the tubingas this might cause obstruction of the tubing.

Securely fasten the tubing to the waste vessel.3

CAUTIONMake sure that the waste vessel will hold all the produced volumeof the run.


4 Installation4.3 Prepare waste tubing for Fraction collector F9-C

4.4 Set the delay volume

IntroductionThe delay volume settings are used to make sure that the fractions collected duringfractionation, using the outlet valve or the fraction collector, correspond to the fractionsindicated in the chromatogram.

Set the delay volumeFollow the instructions below to set the delay volume between the UV monitor and theoutlet valve and between the UV monitor and the fraction collector. Refer to the purifca-tion instrument User manual for more information about delay volumes.

ActionStep

Select System:Settings in the System Control module.1

Result: The System Settings dialog opens.

• Select Tubing and Delay Volumes and selectDelay volume: Monitor tooutlet valve.

• Type in the volume in the Volume field and click OK.

2


4 Installation4.4 Set the delay volume

ActionStep

In the System Settings dialog:3

• SelectDelay volume:Monitor to outlet valve orMonitor to frac accord-ing to the configuration you are using.

• Type in the volume in the Volume field and click OK.

Note:

The system will use the delay volume appropriate to the configuration usedand ignore other settings (e.g. the value forMonitor to outlet valve will beignored if you are using a fraction collector). It is however recommended toset all delay volumes so that the volumes remain correct if you change frac-tionation method.


4 Installation4.4 Set the delay volume

4.5 Performance testBefore taking the Fraction collector F9-C and Fraction collector F9-R instrument intouse, run a performance test to check the function of the equipment. See the purifcationinstrument User manual for further instructions.


4 Installation4.5 Performance test

5 Operation

About this chapterThis chapter describes how to prepare and assemble the fraction collector before a run.

In this chapter

See pageSection

715.1 Prepare Fraction collector F9-C



5 Operation

5.1 Prepare Fraction collector F9-C

About this sectionThis section describes how to prepare and assemble Fraction collector F9-C before arun.

The fraction collector is connected to ÄKTA pure and controlled by UNICORN. Control ofthe fraction collector can be achieved automatically in a method run, or manually.

CAUTIONFire Hazard. Do not fractionate flammable liquids using Fractioncollector F9-C. When running RPC methods, or other proceduresusing organic solvents, collect fractions through the outlet valveor Fraction collector F9-R.

The tray and racks can tilt slightly when not fully inserted into the fractioncollector and may harm the fractionation arm. The tilt is due to the height dif-ference between the door and the floor of the fraction collector. The tendencyto tilt is affected by the placement andweight of the cassettes, tubes or bottles

Note:

Prepare the fraction collectorBefore starting to prepare Fraction collector F9-C, check the fractionation settings in themethod to be run. Perform the steps described below according to the settings in themethod.

• Insert the Cassette tray or a rack for tubes or bottles.

• Change the System Settings in UNICORN to set the fractionation mode and othersettings for fraction collection.

How to insert a tray or a rack is shown below.

For information on how to change the System Settings before a run, see[[ManualNameSystemControlShort]]. The available SystemSettings are described in theÄKTA pure User manual.


5 Operation5.1 Prepare Fraction collector F9-C

Prepare and insert the Cassettetray

Follow the instructions below to add cassettes to the Cassette tray and insert the trayinto the fraction collector.

ActionStep

If you are to use cassettes with the QuickRelease function, open the cassettes.For more information on the QuickRelease function see the ÄKTA pure Usermanual.

1

Place the tubes and deep well plates in the cassettes. Make sure that thedeep well plates are rotated so that the well marked A1 is positioned abovethe A1 marking on the cassette.

2



ActionStep

Close the cassettes that have the QuickRelease function.3

Place the cassettes on the Cassette tray. Make sure that the cassette typecode (see illustration below) faces the front of the tray marked with the GElogo.

4

Open the door of the fraction collector using the handle.5



ActionStep

Insert the tray into the fraction collector:6

• make sure that the front of the tray (marked with the GE logo) facesoutwards

• position the tray and slide it into the fractionation collector until itreaches the end

• make sure that the Tray catch snaps into closed position, as shown be-low.

Note:• The tray can tilt slightly when not fully inserted into the fraction collector

andmay harm the fractionation arm. The tilt is due to the height differencebetween the door and the floor of the fraction collector. The tendency totilt is affected by the placement and weight of the cassettes.

• A height exclusion bar ensures that the tubes or deep well plates arecorrectly positioned and cannot damage the Dispenser head.



ActionStep

Close the door. Make sure that it closes properly.7

Result: After the door has been closed, the fractionation arm scans the cas-sette type code of each cassette to identify the cassette types. If deep wellplates are used, the instrument also identifies the types of deep well plates.

Note:

If the tray is inserted with the front of the tray facing the wrong way it willnot be possible to close the door.

Prepare and insert a rack fortubes or bottles

Follow the instructions below to insert a rack for tubes or bottles into the fraction collector.

ActionStep

Place the tubes or bottles in the rack.1

Open the door of the fraction collector using the handle.2



ActionStep

Insert the rack into the fraction collector:3

• make sure that the front of the rack (marked with the GE logo) facesoutwards

• position the rack and slide it into the fractionation collector until itreaches the end

• make sure that the Tray catch snaps into closed position, as shown be-low.

Note:• The rack can tilt slightly when not fully inserted into the fraction collector

andmay harm the fractionation arm. The tilt is due to the height differencebetween the door and the floor of the fraction collector. The tendency totilt is affected by the placement and weight of the tubes or bottles.

• A height exclusion bar ensures that the tubes or bottles are correctly po-sitioned and cannot damage the Dispenser head.

• Do not use the Cassette tray when the Rack for 50 ml tubes or the Rackfor 250 ml bottles is placed in the fraction collector.



ActionStep

Close the door. Make sure that it closes properly.

Note:

If the rack is inserted with the front of the rack facing the wrong way it willnot be possible to close the door.

4




About this sectionThis section describes how to prepare and assemble Fraction collector F9-R before arun.

The Fraction collector F9-R is connected to either ÄKTA pure or ÄKTA avant and controlledby UNICORN. Control of the fraction collector can be achieved automatically in a methodrun, or manually.

Prepare the fraction collectorBefore starting to prepare Fraction collector F9-R, check the fractionation settings in themethod to be run. Perform the steps described below according to the settings in themethod.

• Assemble the tube rack

• Insert collection tubes

• Adjust the Delivery arm

• Change the System Settings in UNICORN to set the fractionation mode and othersettings for fraction collection.

It is shown how to assemble the tube rack, insert collection tubes and adjust the deliveryarm below. For more information on how to change the System Settings before a run,see either the ÄKTA pure User manual or the ÄKTA avant User manual.


5 Operation5.2 Fraction collector F9-R

Assemble the Tube rackFollow the instructions below to assemble the Tube rack. See either the ÄKTA pure Usermanual or the ÄKTA avant User manual for information about which Tube rack parts touse.

ActionStep

Insert the Tube support (4), if required, into the bowl (3). The circular markson the Tube support should face down.

1

1

2

3456

Note:

When assembling a Tube rack, Single cutouts (1) and L-shaped cutouts (2),are used for various inserts depending on the length of the collection tubes.See the either the ÄKTA pure User manual or the ÄKTA avant User manualfor detailed information.

Insert the Tube guide (5) with the tube position numbers upwards. The Tubeguide should rest about 1 cm above the Tube support.

2



ActionStep

Insert the Tube holder (6) with the tube position numbers upwards:3

• Check that tube position 1 (7) is directly above tube position 1 (7) of theTube guide.

7

• Push the flexible bowl out at each rib and snap the Tube holder underthe top lip of the rib (8).

8

Note:

Do not force the tube holder into place as this may damage the lip.

• Check that the surface of the Tube holder is level.

Gently move the Delivery arm (10) out to the outer stop.4



ActionStep

Place the Tube rack (9) over the Central spindle (11) and pull the springloaded Drive sleeve (12) out so the Tube rack comes to rest.

5

9

1012

11

Insert collection tubesInsert sufficient collection tubes in to the Tube rack, starting at position 1, pushing eachone down as far as they will go. All the tubes must be of the same length and diameterand there should be no empty positions in the sequence.



Adjust the Delivery armFollow the instructions in the table below to adjust the Delivery arm.

ActionStep

Gently lift and then lower the Delivery arm (1), and allow it to move in so theTube sensor (2) touches the collection tubes of the outer track.

1

1

2



ActionStep

Adjust the height of the Delivery arm as follows:2

• Loosen the lock knob (3)

3

• Adjust the height so that the horizontal mark (5) on the Tube sensor (6)is at the same level as the top of the flat collection tubes and approxi-mately 2 mm over the top of the flanged collection tubes (4).

4

5

6

• Lock the Delivery arm at this height with the lock knob.



ActionStep

Check that the Tube sensor (9) is in the correct position for the used tubes(10). The eluent tubing (7) should be above the center of the collection tube.Use the Sensor control (8) to position the Tube holder.

3

7

8

9

10



6 Maintenance

About this chapterThis chapter describes maintenance and provides instructions for how to clean Fractioncollector F9-C and Fraction collector F9-R. For further information, see the purifcationinstrument User manual.


In this chapter

See pageSection

866.1 Clean Fraction collector F9-C



6 Maintenance

6.1 Clean Fraction collector F9-C

Maintenance intervalClean the Fraction collector when required, for example if liquid has been spilled in theFraction collector chamber. The internal tubing of the fraction collector may need to bereplaced for maintenance or for process purposes. Information on how and when toreplace the internal tubing can be found in the ÄKTA pure User manual.

Required materialThe following materials are required:

• Wash bottle

• Water or 20% ethanol

• Cloth

InstructionFollow the instruction below to clean the interior of the Fraction collector. The locationsof the components of the fraction collector are shown in Section 3.1.2 Fraction collectorF9-C illustrations, on page 32.

ActionStep

Perform a fraction collector wash:1

In System Control, select Manual:Execute Manual Instructions:Fractioncollection:Fraction collector wash. Click Execute.

NOTICE

• If no column valve is used, make sure to replaceany columns in the flow path with tubing before afraction collector wash is performed.

• Lower the flow for the fraction collector wash inthe instruction Fraction collector wash settingsif the system back pressure is elevated during thewash.

• Make sure not to exceed the pressure limits for anyof the modules that are part of the flow path.


6 Maintenance6.1 Clean Fraction collector F9-C

ActionStep

In System Control, select Manual:Execute Manual Instructions:Fractioncollection:Frac cleaning position . Click Execute.

2

Result: The Dispenser head moves to cleaning position, and the Instrumentdisplay states System pause.

Open the door of the fraction collector and remove the rack3

Wash the Cassette tray or rack and the Cassettes (if applicable), with waterand a mild cleaning agent.

4

Lift off the Waste funnel and wash it with water and a mild cleaning agent.5

Refit the Waste funnel.

Wipe off the interior of the fraction collector using a damp cloth. Wipe offstains using a mild cleaning agent or 20% ethanol.

6

Wipe off the Dispenser head and its diode windows (the Drop sync sensorand the Type code reader) using a wash bottle with water or 20% ethanoland a cloth.

7

Let the fraction collector dry completely before starting a run.8

Close the door of the fraction collector.9

Result: Automatic scanning is performed.

In the System Control module, press the End icon in the toolbar.10

Result: The Dispenser head moves to home position.


6 Maintenance6.1 Clean Fraction collector F9-C


Maintenance intervalClean the Fraction collector when required, for example in case of liquid spill.

Required materialThe following material are required:

• Water or 20% ethanol

• Cloth

InstructionFollow the instructions below to clean the instrument externally.

ActionStep

Check that no run is in progress.1

Switch off the instrument.2

Wipe the surface with a damp cloth. Wipe off stains using a mild cleaningagent or 20% ethanol. Wipe off any excess.

3

Let the Fraction collector F9-R dry completely before restart.4


6 Maintenance6.2 Fraction collector F9-R

7 Reference information

See the relevant purification instrument Product information and User manual for infor-mation on the following:

• Technical specifications

• Chemical resistance

For information on operating conditions, see Section 4.1.3 Site requirements, on page55.

Environmental ranges

DataParameter

-25°C to +60°CStorage and transport temperature range

See the relevant purification instrumentUser manual.

Chemical environment

Equipment noise level

Acoustic noiselevel

Equipment

< 60 dB AFraction collector F9-C

< 60 dB AFraction collector F9-R

Heat output

Heat outputEquipment

Typically 6 WFraction collector F9-C

Maximum 18 W

2 WFraction collector F9-R


7 Reference information

IndexCCassettes, 36CE

conformity, 9marking, 9

DDelay volume

set, 67Dimensions

instrument, 54

EEmergency procedures

emergency shutdown, 24

FFCC compliance, 11Fractionation

assemble tube rack, 47Fraction collector

cleaning, 86Fraction collector F9-C

Cassettes and racks, 36connect tubing, 40, 63requirements on deep wellplates, 38requirements on tubes, 38Tubes, 46

Fraction collector F9-Rcleaning, 88connect tubing, 48, 64Delivery arm, 82Insert collection tubes, 81Tube rack, 79

IImportant user information, 6Installation

performance test, 69Instrument overview

Fraction collector F9-R, 44

LLabels, 21

Fraction collector F9-C in-strument label, 22

MManufacturing information, 8

NNotes and tips, 7

PPrepare the system

waste tubing, 65Prerequisites, 6Purpose of this manual, 5

RRacks, 36Recycling information

decontamination, 26

SSafety notices, 7Safety precautions, 14

emergency procedures, 24labels, 21

Standards, 12Storage, 51

TTube rack

assembly, 47Typographical conventions, 5

WWaste tubing

prepare, 65Weight

instrument, 53


Index

GE, the GE Monogram, ÄKTA, UNICORN and Whatman are trademarks of GeneralElectric Company.

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fraction collector f9-c and f9-r operating instructions

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