prepfctm fraction collector (ethernet) user’s guide

PREPFCTM Fraction Collector (Ethernet)User’s Guide

Table of Contents

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

1 Introduction

Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2Standard Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Customer Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5

Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

2 Installation

Tray Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2Tray Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3Rack Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

3-Way Valve Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6Dispense Tube and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

System Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7

Plumbing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8Plumbing the Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9Plumbing Multiple Fraction Collectors . . . . . . . . . . . . . . . . . . . 2-11Cable Clip Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15Drain Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15

Rear Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16Ethernet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17Input/Output Ports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18PREPFC Contact Interface Module . . . . . . . . . . . . . . . . . . . . . . . . . 2-21Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-23Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-23

3 Operation

Start Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Run Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2Programmable Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3Remote Inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5Collection Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

4 Maintenance

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Transporting the Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Replacing Dispense Tube and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . 4-4

Replacing the 3-Way Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Before you Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Remove the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Replace the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6Adjust the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6

Replacing a Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7

5 Troubleshooting

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Repair and Return Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Before Calling Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Non-Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Rebuilt Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Return Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6Unit End-of-Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6

A Replacement Parts and Accessories

B Racks

C System Organizer

Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1

Attaching the Shelf Stiffeners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2

Attaching the Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2

Attaching the Corner Braces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3

D Pocket PC Guard

Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1

Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 1-1

Introduction 1

The PREPFCTM Fraction Collector (Ethernet) is a small-footprint preparative fraction collector with a 3-way valve for flow rates up to 200 mL/min. The PREPFC has an adjustable tray platform that can be installed at four different heights and can accommodate vessel heights up to 230 mm. Up to four PREPFC Fraction Collectors can be connected to increase capacity.

The PREPFC Fraction Collector is controlled by Gilson PREPFCTM Control Software which is run on a handheld Pocket PC running Microsoft® Windows Mobile®.

Introduction 1

1-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

Un

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g Unpacking

Unpack the fraction collector and accessories carefully from the carton. Cross-check the contents against the Standard Equipment on page 1-3 and against your purchase order’s optional accessory list to verify that all parts are included and undamaged.

Do this now, even if the fraction collector will not be used immediately. Many carriers must receive concealed damage claims within seven days of delivery.

The fraction collector is delivered with most major components already assembled. Keep the original container and packing assembly in case the fraction collector must be returned to the factory.

To unpack the fraction collector:

1 Open the box and remove the cardboard insert from the top of the box.

2 There are two boxes inside, one contains the tray platform and insert (if ordered), and the other contains the accessories. Remove these boxes.

3 Grab the two handle cutouts on the cardboard insert and lift the fraction collector up and out of the box.

4 Lift the fraction collector off the cardboard insert.

Do not attempt to lift the instrument from the arm. You may carry the fraction collector using the large rectangular opening in the front of the unit.


Introduction 1U

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Standard Equipment

Once the fraction collector and accessories are unpacked, you should have the following:

• PREPFCTM Fraction Collector (with 3-way valve and dispense tube for 1/8" OD tubing installed)

• Tray platform

• Accessory package which includes Cat 5e ethernet cable, barbed adapter (for drain tubing), terminal block connector, fuses, fuse drawers, power cords, headless nut extender tool, dispense tube for 1/16" OD tubing, and Gilson Ethernet Utility

Documentation

The following documents are included with the fraction collector:

• PREPFCTM Fraction Collector (Ethernet) Documentation CD

• IQ Procedure PREPFCTM Fraction Collector (Ethernet)

• Unpacking the PREPFCTM Fraction Collector (Ethernet)

• Declaration of Conformity

Introduction 1


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Based upon your configuration, you’ll also receive additional accessories, ordered separately.

Tray Inserts

Plumbing Packages

Multiple PREPFC Fraction Collectors

Control

To control the fraction collector you will need Gilson PREPFC Control Software.

You will also need a router, a Pocket PC, an Ethernet Compact Flash Card for Pocket PC*, and a Cat 5e ethernet cable*. They are not available for purchase from Gilson, Inc.

Optionally, purchase a Pocket PC Guard for HP iPAQ hx2100/2400-Series (part number 21052609).

* Not required if using a wireless router.

Part Number Description

17007112 Insert for four Code 20-series

17007113 Insert for two Code 200-series racks

17041950 Code 91 rack for eleven 500 mL bottles

17041951 Code 92 rack for eight 1000 mL bottles


1747995 PREPFC Plumbing Package 1/8" OD

1747996 PREPFC Plumbing Package 1/16" OD


17110101 PREPFC Fraction Collector (Ethernet)

17017103 PREPFC Contact Interface Module to connect up to four fraction collectors

17027114 Cable to connect from PREPFC Contact Interface Module to each fraction collector (one cable required for each fraction collector)

210526 System Organizer, PREPFC


Introduction 1C

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Customer Service

Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following types of assistance: sales, technical support, applications, and instrument repair.

If you need assistance, please contact your Gilson-authorized representative. Specific contact information can be found on the Gilson website at www.gilson.com. To help us serve you quickly and efficiently, please refer to Before Calling Us on page 5-5.

http://www.gilson.com

Introduction 1


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Please be aware of the following before operating the fraction collector.

Warning! Changes or modifications to the fraction collector not expressly approved by Gilson could void the factory-authorized warranty.

The fraction collector has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC commercial environment. The fraction collector generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. Operation of the fraction collector in a residential area is likely to cause harmful interference; in which case, the user will be required to correct the interference at the user’s own expense.

Shielded cables must be used with the fraction collector to ensure compliance with the Class A FCC limits.


Introduction 1Tech

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Technical Specification Definition

3-way valve Three-port, PTFE, 114 μL internal volume, 10 μL dead volume, up to 200 mL/min

Operating pressure: 30 psi on the NO and NC port, 60 psi on the COMM port

Valve switching speed < 20 msec

Analog input (detector) ±1000 mV

Contact control Contact closure inputs for remote START, STOP, ADVANCE, and DIVERT

Two programmable outputs (which include an event mark output)

Data acquisition An input channel for analog to digital conversion

Environmental conditions Indoor use

Altitude: up to 2000 m

Temperature range: 5–40°C

Air pressure: 75–105 kPa

Pollution degree: 1 or 2, in accordance with IEC 66

Humidity: Maximum relative humidity 80% for temperatures up to 31° C, decreasing linearly to 50% relative humidity at 40°C

Manufacturing standards Meets applicable Safety and EMC certification standards; UL, CSA, and CE certified

Maximum collection volume/tube

1000 mL (Kimax or Pyrex bottles with Code 92 rack)

Multi-cycle operation With PREPFCTM Control Software: Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes

Number of racks Up to four Code 20-, Code 33X/34X-, or Code 30-series racks

Up to two Code 200-series racks

Or one Code 90-series rack

Operating modes Use PREPFCTM Control Software to collect by Time, Volume, Slope, or Level

continued (1 of 2)

Introduction 1


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Maximum fractions Qty Capacity Gilson rack (qty)

320 13 x 100 mm (9 mL) test tubes Code 343 rack (4)

192 16 x 100 mm tubes Code 223 rack (2)

176 18 x 150 mm test tubes Code 22 rack (4)

80 25 x 200 mm test tubes Code 239 rack (2)

54 28 x 57 mm (25 mL) scintillation vials

Code 204 rack (2)

54 30 x 115 (50 mL)conical bottom tubes

Code 222 rack (2)

18 48 x 113 mm (125 mL) bottles Code 211 rack (2)

11 500 mL bottles Code 91 rack (1)

8 1 liter bottles Code 92 rack (1)

For a full list of available racks refer to Appendix B, Racks

Peak detection Use PREPFCTM Control Software to collect by Slope or Level

Physical space requirement (W x D x H)

50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in)

Power requirements Frequency: 50/60 Hz

Voltage: 100–240V, mains voltage fluctuations not to exceed 10% of the nominal voltage

Current rating: 0.50A for 100–120V

0.25A for 220–240V

Software control Stand-alone control via Ethernet using a Pocket PC running Microsoft® Windows Mobile® and PREPFCTM Control Software

Tube change time 100–250 msec, center-to-center, depending on rack type

Weight 8.6 kg (19 lbs)*

*Weight includes the tray platform

Technical Specification Definition

continued (2 of 2)


Installation 2

This section takes you through the steps for setting up your fraction collector.

tray platform

tray insertracks

3-way valve

drain

barbed

inlet tubing

outlet tubing

tubing

adapter

spiral wrap

(from detector)

(to waste)

Installation 2


Tray

Set

up Tray Setup

The fraction collector is equipped with an adjustable tray platform to hold the racks. All of the racks require the use of the tray platform.

To install the tray platform:

1 Determine the height of the rack with the installed vessels to be used to collect fractions.

2 Refer to the table below and the diagram on the left to determine the position of the tray platform.

3 Align the tray platform with the two screws on each side of the fraction collector. The notches on the top sides of the tray platform should rest below the screw on the top and the notches on bottom should rest on top of the bottom screw.

CAUTION! Before changing the height of the tray platform, remove any inserts or racks that are present.

Position Total height(rack + vessels)

A up to 80 mm

B up to 130 mm

C up to 180 mm

D up to 230 mm

A

B

C

D

right side view offraction collector

tray platform notches


Installation 2Tray Setu

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Tray Inserts

A tray insert is used to hold racks in place on the fraction collector. There are two inserts available (ordered separately) depending on the type of racks used.

• Insert for two Code 200-series racks (part number 17007113)

• Insert for four Code 20-series racks (part number 17007112)

The Code 20-series tray insert is used for Code 20-series, Code 33X/34X-series, or Code 30-series racks

The Code 90-series racks do not use a separate tray insert, they sit directly on the tray platform.

To install a tray insert or Code 90-series rack, insert the drain fitting on the right side of the tray insert into the hole on the right support of the fraction collector.

Note: When using multiple fraction collectors, the same tray insert (or Code 90-series rack) must be used on all fraction collectors.

Installation 2


Tray

Set

up Rack Installation

The fraction collector can be configured with a variety of rack types and sizes. Refer to Appendix B, Racks for information on the available racks.

Code 20-series, Code 33X/34X-series, or Code 30-series racks

The Code 20-series, Code 33X/34X-series or Code 30-series racks are placed perpendicular to the back of the fraction collector.

1 Orient the rack so that the code number is facing forward.

2 Locate the middle slot on the back of the rack. Slide this over the pin on the tray insert.

3 Fit the middle slot on the front of the rack over the pin in the front of the tray insert.

Note: If Code 30-series racks will be used with the thermostating cuvette, a spacer for the thermal rack (part number 17007115, ordered separately) is required to raise the height of the rack and thermostating cuvette.

Code 20-series tray insert


Installation 2Tray Setu

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Code 200-series racks

The Code 200-series racks are placed parallel to the back of the fraction collector.

1 Orient the rack so that the code number is facing the right side.

2 Fit the rack on the tray insert so that the slots and holes on the underside of the rack align with the pins on the tray insert.

Code 200-series tray insert

Code 90-series racks

The Code 90-series racks sit directly on the tray platform. To install a Code 90-series rack, insert the drain fitting on the right side of the tray insert into the hole on the right support of the fraction collector.

Code 91 rack

Installation 2


3-W

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The fraction collector is shipped with the valve already installed. Ensure that the adapter end of the valve cable is connected to the fraction collector.

Dispense Tube and Fittings

The fraction collector is shipped with the dispense tube for 1/8" OD tubing installed. If you will be using the 1/16" OD plumbing package (part number 1747996) it is recommended to use the dispense tube for 1/16" OD tubing, included in the accessory package.

To switch dispense tube and/or dispense tube fittings:

1 Slide the 3-way valve assembly to the front of the X/Y arm.

2 Locate the headless nut extender tool (part number 49041032) included in the accessory package.

3 Place this tool over the dispense tube and fittings and turn clockwise to loosen.

4 Remove the old dispense tube and fittings and replace with the new ones. Use the headless nut extender tool and tighten until snug.

dispense

fittings

tube

IN

DIVERT


Installation 2System

Setup

System Setup

Refer to the following diagram for information on setting up multiple fraction collectors using the PREPFCTM Fraction Collector System Organizer (ordered separately, part number 210526). For instructions on assembling the system organizer refer to Appendix C, System Organizer.

If you will be using more than one fraction collector you will need the PREPFC Contact Interface Module. For information on making these connections refer to PREPFC Contact Interface Module on page 2-21.

FC 1FC 2

FC 3 FC 4

Installation 2


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s Plumbing Connections

The tables and diagrams in the following sections provide detailed information on making plumbing connections.

• For information on plumbing one fraction collector refer to page 2-9.

• For information on plumbing multiple fraction collectors refer to page 2-11.

Before making the connections, locate one of the following plumbing packages (ordered separately). You will need one plumbing package for each fraction collector.

Part number Qty. Description

The PREPFC Plumbing Package 1/8" OD (part number 1747995). This package includes:

49010106 2 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing

49010107 2 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing

49010108 4 Ferrule, Tefzel, 1/4–28 for 1/8" OD tubing

541180372 3 ft Spiral wrap, 3/8" diameter, black

49040681 1 Upchurch adapter (P-681), 5/16–24 female to 1/4–28 male, Kel-F (PTCFE)

470512503 15 ft PTFE tubing, 1/8" ID x 3/16" OD

49041038 1 Upchurch flangeless nut (P-138), 3/16"/4 mm, 5/16–24, Delrin (Acetal)

49041039 1 Upchurch flangeless ferrule (P-133), 3/16", Tefzel (ETFE), blue

490032 15 ft PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot

54083751 3 Retainer, 0.375" diameter, adhesive

54096037 1 Cable clip, 0.375" diameter

470350006 10 ft Tygon drain tubing, 3/8 x 1/2

Or, the PREPFC Plumbing Package 1/16" OD (part number 1747996). This package includes:


49010111 2 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing


541180372 3 ft Spiral wrap, 3/8" diameter, black

490032 15 ft PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot



470504001 15 ft PTFE tubing, 0.04" ID x 1/16" OD, natural

54083751 3 Retainer, 0.375" diameter, adhesive

54096037 1 Cable clip, 0.375" diameter

470350006 10 ft Tygon drain tubing, 3/8 x 1/2


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Plumbing the Fraction Collector

Using the PREPFC Plumbing Package 1/8"

Refer to the following diagram and table for instructions on making the plumbing connections for one fraction collector using the PREPFC Plumbing Package 1/8" (part number 1747995).

Use this package for flow rates between 30 and 200 mL/min.

Plumbing Connections—1/8"

Valve Connection

Tubing Fitting(s)

INLET

COMM (Common) port from detector

PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)

On one end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.

On the other end of the tubing use the appropriate fitting to connect to the outlet of the detector.

DIVERT position

NO (Normally Open) port to waste

PTFE tubing, 1/8" ID x 3/16" OD (part number 470512503)

On one end of the tubing, use an Upchurch P-138 flangeless nut (3/16", 5/16-24, part number 49041038) and an Upchurch P-133 flangeless ferrule (3/16", part number 49041039). Connect this end to an Upchurch P-681 adapter, 5/16–24 female to 1/4–28 male (part number 49040681) then connect this to the DIVERT port.

COLLECT position

NC (Normally Closed) port

Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)

Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).

Installation 2


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s Using the PREPFC Plumbing Package 1/16"

Refer to the following diagram and table for instructions on making the plumbing connections for one fraction collector using the PREPFC Plumbing Package 1/16" (part number 1747996).

Use this package for flow rates up to 30 mL/min.


Valve Connection

Tubing Fitting(s)

INLET

COMM (Common) port to detector

PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)



DIVERT position



On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 490410106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.

COLLECT position


Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)

Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)


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Plumbing Multiple Fraction Collectors


The following diagram shows the connections if four fraction collectors are connected using the PREPFC Plumbing Package 1/8" (part number 1747995). Refer to the table on page 2-12 for more detailed instructions on making these connections. The maximum flow rate when plumbing multiple fraction collectors with this package is 170 mL/min.

Installation 2


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Valve Connection

Tubing Fitting(s)

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INLET





DIVERT position

NO (Normally Open) port to COMM (Common) port on FC 2



On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port on FC 2.

COLLECT position




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INLET

COMM (Common) port to NO (Normally Open) port on FC 1


On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port on FC 1.

On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.

DIVERT position





COLLECT position




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INLET





DIVERT position





COLLECT position




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INLET





DIVERT position


PTFE tubing, 1/8" ID x 3/16" OD (part number 470512503)

On one end of the tubing, use an Upchurch P-138 flangeless nut (part number 49041038) and an Upchurch P-133 flangeless ferrule (part number 49041039). Connect this end to an Upchurch P-681 adapter, 5/16–24 female to 1/4–28 male (part number 49040681) then connect this to the DIVERT port.

COLLECT position





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The following diagram shows the connections if four fraction collectors are connected using the PREPFC Plumbing Package 1/16" (part number 1747996). Refer to the table on page 2-14 for more detailed instructions on making these connections. The maximum flow rate when plumbing multiple fraction collectors with this package is 25 mL/min.

Installation 2


Plu

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s


Valve Connection

Tubing Fitting(s)

Fra

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ect

or

1

INLET





DIVERT position



On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port.

On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port on FC 2.

COLLECT position




Fra

ctio

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ect

or

2

INLET



On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port on FC 1.

On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.

DIVERT position





COLLECT position




Fra

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oll

ect

or

3

INLET





DIVERT position





COLLECT position




Fra

ctio

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oll

ect

or

4

INLET





DIVERT position




COLLECT position





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Cable Clip Installation

To keep the tubing from sliding around with arm movement you may want to use the cable clip and guides (adhesive retainers) that were included in the plumbing package. A sample configuration with four fraction collectors on the PREPFC Fraction Collector System Organizer (ordered separately, part number 210526) is shown below.

rear view—System Organizer with four PREPFC Fraction Collectors

Drain Connections

To make the drain connections:

1 Connect the barbed adapter (part number 49081701, included in the accessory package) to the outlet on the right support of the fraction collector.

2 Connect one end of the drain tubing (part number 470350006, included in the plumbing package) to the barbed adapter and place the other end into a drain or container.

Note: Position the drain/drain tubing to allow for continuous flow.

to

from

FC 1 FC 2

FC 3FC 4

detector

waste

Installation 2


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s Rear Panel Connections

Rear Panel

Please read this entire section and refer to the rear panel diagram below before making any electrical connections. Do not turn on power to the fraction collector until all connections have been made.

1 Ethernet

2 Input/Output (I/O) ports

3 Power receptacle

4 Power switch

5 Fuse drawer

1

2

3 4 5


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Ethernet

To make the Ethernet connection to the instrument, you will need a router and Cat 5e ethernet cables.

Note: The router is not available from Gilson, Inc.

1 Connect the router to your company's network (optional)

Connect one end of a Cat 5e ethernet cable to the company network and the other end of the cable to the WAN port on the back of the router. Please consult with your System Administrator or IT Professional.

2 Connect the power supply to the router

Connect the power supply to the router. At this time, do not connect to the power source.

3 Connect the instrument to the router

Warning! Before connecting, ensure that the instrument is powered OFF.

Locate the Cat 5e ethernet cable that was included in the accessory package. Plug one end of the cable into the Ethernet port on the instrument and the other end to one of the numbered ports on the back of the router.

Installation 2


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s Input/Output Ports

You can take advantage of the powerful built-in features of your new fraction collector when you connect other equipment to the multi-purpose Input/Output (I/O) port.

The port has 14 contacts, numbered 1–14. All of the connections are paired contact closures (no voltage) except for the ANALOG IN input. The voltage range for the ANALOG IN input is from –1000mV to +1000mV.

TTL signals can be used as long as the voltage is no greater than 5V.

Some uses for the I/O contact pairs are listed below.

• When you connect a remote switch contact to the START (1–2 pair) or the STOP input (3–4 pair), other equipment can signal the fraction collector to start or stop a run.

• When you connect a remote switch contact to the ADVANCE input (5–6 pair), other equipment can signal the fraction collector to advance during a run.

• When you connect a remote switch contact to the DIVERT input (7–8 pair), other equipment can signal the fraction collector to divert (or collect) during a run.

• When you connect other equipment to the PROG 1 (9–10 pair) or the PROG 2 (11–12 pair) programmable outputs, the fraction collector can give instructions to that device based on options (e.g., Event Mark) available in PREPFC Control Software.

• When you connect a detector to the ANALOG IN input (13–14 pair), the fraction collector can collect peak-containing fractions. Refer to Making connections to a detector on page 2-20 for more information.

The I/O connection diagram shows the location of the multipurpose I/O contacts. Make the I/O connections to the terminal block connector that attaches directly to the fraction collector.


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Making connections

To make connections, you’ll need the following:

• terminal block connector

• 2-conductor cable (22–30 gauge for each wire)

• wire insulation stripper

• small-blade screwdriver

You can purchase a 6-foot piece of suitable cable (part number 709910206) or a package of five cables with identification markers (part number 36078155) from Gilson.

To make connections with the 2-conductor cable:

1 Cut the cable into pieces of appropriate length.

2 Strip about 3 mm of insulation from each end of the cable.

3 Remove the terminal block connector from the fraction collector. Insert each wire into the appropriate slot on the terminal block connector.

Note: When making connections, be sure to maintain the correct orientation of the connector relative to the port.

Push the wire all the way in; then tighten its corresponding pin screw.

4 Reconnect the terminal block connector to the fraction collector. The wires will be facing down and the pin screws facing away from the fraction collector. Push the connector in as far as it will go. It is designed to fit snugly into its receptacle.

5 Connect the opposite ends of the wires to the other equipment. Be sure to match ground connections.

Remote inputs - When making the connections, the source must provide a signal between +5V and ground or a correctly polarized TTL (0,5V) compatible signal (0 activates the input).

Programmable outputs - To use the programmable outputs, connect the leads from PROG 1 (9–10 pair) or PROG 2 (11–12 pair) to a contact-activated input of another device. Connect either lead to either input pin on the peripheral device.

ANALOG IN input - If you are connecting the ANALOG IN input to a detector, refer to Making connections to a detector on page 2-20 for more information.

6 Label each cable to identify the purpose of the connection.

terminal blockconnector

2-conductorcable

to peripheralequipment

Installation 2


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s Making connections to a detector

Detectors generally have one of two types of output connectors. Using the accompanying figures, determine which output connectors are used on your detector.

One type of detector has screwable binding posts built into the rear panel of the detector. To attach the fraction collector to these outputs, connect bare wire lead 14 to the “+” post on the detector and lead 13 to the GND (ground) post. Tighten the posts securely.

The other type of connector, like the one on most Gilson detectors, has fixed binding receptacles. To attach this type, you’ll need a Pomona cable adapter (part number 6374022611).

Make the connections to your fraction collector as shown below. Be sure to connect lead 14 to “+” and lead 13 to GND.

(+) (–)

to fraction collector’sANALOG IN input

(13-14 pair)

detector

Pomona cable adapter

(–)

(+)

black

red

to fraction collector’s

to recorder

to fraction collector’s

detector

to fraction collector’sANALOG IN input

(13-14 pair)

ANALOG INinput 14 (+)

ANALOG INinput 13 (–)


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PREPFC Contact Interface Module

To connect multiple fraction collectors you will need a PREPFC Contact Interface Module (part number 17017103, ordered separately). For each fraction collector you will need a Contact Interface Module cable (part number 17027114, ordered separately). Up to four fraction collectors can be connected using the PREPFC Contact Interface Module.

1 TO FC

2 Input/output ports

1 1

11

2

Installation 2


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s To make connections to each fraction collector:

1 Attach the end of the cable with the terminal block connector to the input/output ports on the fraction collector. The cable should be coming out on the right side of the connector.

2 Attach the other end of the cable to one of the TO FC ports on the contact interface module. Tighten the retaining screws using a flat-blade screwdriver.

3 Repeat steps 1 and 2 for each fraction collector.

Input/Output ports

The input/output ports on the contact interface module have the same functions as the input/output ports on each fraction collector. For more information refer to Input/Output Ports on page 2-18.

PREPFC Contact Interface Module (top view)

FC 4 rear panel

FC 1 rear panel

FC 3 rear panel

FC 2 rear panel

input/output ports


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Fuses

You received the fraction collector without any fuses installed. To install the fuses:

1 Locate the accessory package containing the fuse drawer appropriate for your line voltage. Discard the other fuse drawer.

2 Locate the 0.50A “T” Slo-Blo fuses (5 x 20 mm size), also included in the accessory package.

3 Install the fuse(s) into the fuse drawer. The fuse drawer for 100–120V accepts one fuse. The fuse drawer for 220–240V accepts two fuses.

4 Insert the fuse drawer into its receptacle in the fraction collector. See rear panel diagram on page 2-16.

Power Connection

Locate the appropriate power cord for your line voltage. Discard the other power cord.

Use the power cord to connect the fraction collector to an AC power source.

fuse installation for 100–120V fuse installation for 220–240V


Operation 3

The PREPFCTM Fraction Collector is controlled by PREPFC Control Software which is run on a handheld Pocket PC running Microsoft® Windows Mobile®. The Pocket PC is the dedicated fraction collector controller.

If you have not already done so, install the software. Refer to the PREPFC Control Software Installation Overview and Instructions (part number LT350021) supplied with the PREPFC Control Software and also found on the PREPFC Control Software Documentation CD (part number LT2106270CD).

Operation 3


Star

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The following steps must be completed in order to operate the fraction collector:

1 Connect the router to a power source and wait 30 seconds.

2 Power ON the fraction collector and wait 1 minute.

If the status light on the front of the fraction collector begins blinking, there is a problem with the fraction collector. See page 5-4.

3 Connect the Pocket PC to the router (not required with a wireless router).*

4 Launch PREPFC Control Software on the Pocket PC.

Run Setup

The PREPFC must be set up using PREPFC Control Software. Refer to the PREPFC Control Software User’s Guide included on the PREPFC Control Software Documentation CD (part number LT2106270CD) for this information.

The remote inputs and programmable outputs are available when the fraction collector is used with other equipment.

• Using the programmable outputs, the fraction collector sends instructions to other equipment. Refer to Programmable Outputs on page 3-3 for more information.

• Using the remote inputs, the fraction collector receives instructions from other equipment. Refer to Remote Inputs on page 3-5 for more information.


Operation 3Start U

p

Programmable Outputs

The fraction collector has several jobs to accomplish during normal operation. It may be waiting, diverting/draining, or collecting. The PROG 1 (9–10 pair) or the PROG 2 (11–12 pair) programmable outputs can be used to direct other equipment (a chart recorder or pump, for example) to be active only when the fraction collector is doing one of those jobs. The programmable outputs are controlled through PREPFC Control Software.

The following diagram illustrates each of the options available for the programmable outputs and assumes the initial contact state is opened (OFF). Refer to the text after the diagram for an explanation of each of these options.

A On After Initial Wait - The state of the contact is closed (ON) at the beginning of the first window and stays closed (ON) until the end of the run or until the run is stopped.

B On During Initial Wait - The state of the contact is closed (ON) at the beginning of the run and then is opened (OFF) when the first collection window starts.

Operation 3


Star

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p C On During Collection Window - The state of the contact is closed (ON) at the beginning of each collection window and then is opened (OFF) at the end of the collection window.

D Pulse at End of Run - Pulses the contact at the end of the run.

E Pulse at Peak Start - Pulses the contact at the start of each peak. The contact is only pulsed when the peak is detected inside a collection window (the other equipment will be idle when outside a collection window). This option applies only to collection by Level or Slope.

F On During Peak - The state of the contact is closed (ON) during the peak and then is opened (OFF) at the end of the peak. The contact is only closed (ON) when the peak is detected inside a collection window (the other equipment will be idle when outside a collection window). This option applies only to collection by Level or Slope.

G Pulse at Valve Switch (Event Mark) - Pulses the contact every time the valve is switched from DIVERT to COLLECT or from COLLECT to DIVERT. The contact is only pulsed when inside a collection window (the contact will be idle when outside a collection window).


Operation 3Start U

p

Remote Inputs

The START, STOP, ADVANCE, and DIVERT inputs can perform the functions listed below.

Note: The run screen in PREPFC Control Software must be open in order for the PREPFC to be controlled using the remote inputs.

START (1–2 pair)

• Remote start of the run.

STOP (3–4 pair)

• Remote stop of the run. The fraction collector will not home.

ADVANCE (5–6 pair)

• Advances to the next tube only during the run.

If the valve is in the DIVERT position, it will remain in the DIVERT position.

If valve is in the COLLECT position, the valve will switch to DIVERT, move to the next tube, and then switch back to COLLECT.

DIVERT (7–8 pair)

• Changing the contact state toggles the valve between the DIVERT and COLLECT positions.

• Switching the valve to DIVERT will prevent any sample from being collected.

• Will advance to the next tube if the current tube has sample.

• Switching the valve to COLLECT will allow sample to be collected, when appropriate.

• Valve will not start collecting when in peak mode unless in a peak.

• Valve will start collecting when in a non-peak mode.

Collection Modes

PREPFC Control Software allows collection by Slope, Level, Time, and Volume. Refer to the PREPFC Control Software User’s Guide included on the PREPFC Control Software Documentation CD (part number LT2106270CD) for information on setting collection mode parameters.


Maintenance 4

To obtain optimum performance and maximum life from the PREPFCTM Fraction Collector, it is important to keep the instrument well-maintained.

This section contains the following information to help you maintain your fraction collector.

• Cleaning

• Transporting the Fraction Collector

• Replacing Dispense Tube and Fittings

• Replacing the 3-Way Valve

• Replacing a Fuse

Maintenance 4


Cle

anin

g Cleaning

The fraction collector should be cleaned occasionally using a dry, clean cloth. Or, if necessary, use a cloth dipped in soapy water. If liquid is accidentally spilled on the fraction collector, wipe the instrument using a dry, clean cloth.


Maintenance 4Tran

spo

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the Fractio

nC

ollecto

r

Transporting the Fraction Collector

When moving the fraction collector to another location, do not use the arm as a handle. Doing so may damage the unit or cause misalignment of the X/Y positioning. You may carry the fraction collector using the large rectangular opening in the front of the unit.

Maintenance 4


Rep

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ng

s Replacing Dispense Tube and Fittings

To replace dispense tube and/or dispense tube fittings:


2 Locate the headless nut extender tool (part number 49041032) included in the accessory package.

3 Place this tool over the dispense tube and fittings and turn clockwise to loosen.

4 Remove the old dispense tube and fittings and replace with the new ones. Use the headless nut extender tool and tighten until snug.

dispense

fittings

tube

IN

DIVERT


Maintenance 4R

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e 3-Way V

alve

Replacing the 3-Way Valve

To replace the 3-way valve, follow the procedures in this section.

Before you Begin

1 Switch power OFF to the fraction collector.


3 Unplug the valve cable from the fraction collector.

4 Remove all fittings and tubing (including the dispense tube) from the valve. For more information on removing the dispense tube refer to Replacing Dispense Tube and Fittings on page 4-4.

Remove the Valve

1 Find the location where the IGUS cable is attached to the track.

2 Grip the IGUS cable on the last link (next to the mounting bracket) and rotate to detach.

3 Remove the valve cable from the IGUS cable and from the guide on the valve bracket.

4 Remove the two screws holding the valve on the valve bracket.

track

overhead view—IGUS cable on track

back of fraction collector

mounting

last link

bracket

of IGUScable

valvecable

screws

valve bracket

Maintenance 4


Rep

laci

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the

3-W

ay V

alve Replace the Valve

1 Reattach the valve to the valve bracket making sure that the DIVERT port is located at the top.

2 Place the valve cable in the guide on the valve bracket. Route the wiring behind the dispense tube (between the dispense tube and the valve bracket).

3 Place the valve cable in the IGUS cable.

4 Place the adapter end of the valve cable on top of the mounting bracket on the IGUS cable.

5 Reassemble the IGUS cable.

6 Plug the adapter end of the valve cable into the fraction collector.

7 Reattach all tubing and fittings (including the dispense tube).

Adjust the Valve

The position of the valve may need to be adjusted slightly after all the tubing and fittings have been replaced.

If the dispense tube is not centered in the opening on the track do the following:

1 Slightly loosen each of the screws on the back of the valve bracket.

2 Adjust the valve so the dispense tube is centered in the opening of the track.

3 While holding the valve in position, tighten the screws on the back of the valve bracket.

DIVERT

IN

dispense tube

valve cable

guide

to IGUS cable


Maintenance 4R

eplacin

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se

Replacing a Fuse

A blown fuse may indicate the existence of another problem in the instrument. If the replacement fuses blow, don’t try others. Contact your local distributor. See Before Calling Us on page 5-5.

To change a fuse, follow these steps.

1 Disconnect the power cord from the power outlet and from the rear panel receptacle.

2 Locate the fuse drawer on the rear panel. See page 2-16 if necessary.

3 Insert a small screwdriver into the notch located to the left of the fuse drawer.

4 Twist the screwdriver to open and remove the fuse drawer.

• The fuse drawer for a 100–120 voltage selection contains one 0.50A “T” Slo-Blo fuse (5 x 20 mm size).

• The fuse drawer for a 220–240 voltage selection contains two 0.50A “T” Slo-Blo fuses (5 x 20 mm size).

5 Remove the old fuse(s) and insert the new fuse(s)

6 Insert the fuse drawer into its receptacle in the fraction collector.


Troubleshooting 5

If you encounter a problem while operating the fraction collector, refer to the following pages. If you cannot solve or isolate the problem, contact your Gilson-authorized representative. See Before Calling Us on page 5-5.

This chapter provides information on the following topics:

• Error Messages

• Troubleshooting

• Repair and Return Policies

Troubleshooting 5


Erro

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essa

ges Error Messages

Error # Error Text Description

0 Normal

10 Bad command An illegal command was received.

11 Range error An out-of-range parameter was received.

30 X Homing The unit was not able to home itself.

31 Y Homing The unit was not able to home itself.

32 X Unhomed A motion was requested when the unit was not homed state.

33 Y Unhomed A motion was requested when the unit was not homed state.

40 Tube Queue error Queue already full.


Troubleshooting 5Tro

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Troubleshooting

Ethernet connections

For communication to occur, the PREPFCTM Fraction Collector must be connected via an Ethernet connection to a router. If there are communication problems between the fraction collector and the Pocket PC, verify that the procedure below has been followed.

1 Connect the router to a power source and then wait 30 seconds for the router to initialize.

2 With the fraction collector powered OFF, connect one end of an Ethernet cable to a numbered port on the back of the router and the other end to the Ethernet port on the rear panel of the fraction collector.

3 Power the fraction collector ON and then wait 1 minute for it to initialize. The fraction collector will be assigned an IP address via DHCP from the router at this time.

4 This step is not necessary with a wireless router.Using a Cat 5e ethernet cable, connect one end of the cable to the connector on the Ethernet CF (Compact Flash) Card for Pocket PC and the other end to a numbered port on the back of the router.

Use the Gilson Ethernet Utility (part number 21067530, included in the accessory package) to further check communication or contact your Gilson-authorized representative.

Troubleshooting 5


Tro

ub

lesh

oo

tin

g Fraction fluid is missing the vessel

• Check to be sure the template and the rack code in PREPFCTM Control Software matches the racks you are using.

• Check location of racks and tray insert; the racks may be out of position on the tray insert or the tray insert may not be seated properly in the tray platform.

• Check mechanical X/Y arm assembly for alignment. Turn the unit off and then restart it. If it is still out of alignment, contact your Gilson-authorized representative.

3-way valve not functioning or functioning incorrectly

• Check connection to the fraction collector. Plugging or unplugging valve with the power on is not suggested as this may cause the machine to reset.

• Check the fluid connections to the valve for proper orientation.

• Contact your Gilson-authorized representative; the valve may be defective.

Unit not operational

• Make sure the power is turned on.

• Check AC power cord connections

• Try a different AC outlet.

• Check fuse(s); replace if necessary.

• Contact your Gilson-authorized representative.

Status light blinking

• Check for obstructions.

• Home the fraction collector.

• Switch the power to the fraction collector OFF then ON.

• Restart controlling program.


Unit blows fuses



Troubleshooting 5R

epair an

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eturn

Policies

Repair and Return Policies

Before Calling Us

Gilson-authorized representatives will be able to serve you more efficiently if you have the following information:

• the serial number and model number of the instruments involved. The serial number is located on the inside of the fraction collector, on the right support. Refer to the diagram.

• the installation procedure you used

• list of concise symptoms

• list of operating procedures and conditions you were using when the problem arose

• list of other devices connected to the fraction collector and a description of those connections

• list of other electrical connections in the room

Warranty Repair

Units covered under warranty will be repaired and returned to you at no charge. If you have any questions about applicability, please contact your local distributor.

Non-Warranty Repair

For out-of-warranty repairs, contact your local distributor. A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment, if necessary.

Rebuilt Exchange

For some units, rebuilt exchange components are available. Contact your local distributor for details.

serial number

Troubleshooting 5


Rep

air

and

Ret

urn

Po

licie

s Return Procedure

Contact your local distributor’s Customer Service Department to obtain authorization before returning any Gilson equipment. To return a piece of equipment:

• Carefully pack the unit to prevent damage in transit. Check with your distributor regarding proper method of shipment. No responsibility is assumed by Gilson or your distributor for damage caused by improperly packaged instruments. Indicate the authorization on the carton and on the packing slip.

• Always insure for the replacement value of the unit.

• Include a description of symptoms, your name, address, phone number, and purchase order to cover repair costs, return and shipping charges, if your institution requires it.

Unit End-of-Life

When a unit reaches the end of its useful life, refer to www.gilson.com for directions and information on the end-of-life policy. This is in accordance with the European Union Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE).

http://www.gilson.com

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide A-1

Replacement Parts and Accessories A

Rack Accessories


17007111 Tray platform

17007112 Tray insert for four Code 20-series racks

17007113 Tray insert for two Code 200-series racks

Valve


17037251 3-way valve, PREPFC

Tubing and Fittings


49010106 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing

49010107 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing

49010108 Ferrule, Tefzel, 1/4–28 for 1/8" OD tubing

49010109 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing

49010111 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing

49010112 Ferrule, Tefzel, 1/4–28 for 1/16" OD tubing

49041038 Upchurch flangeless nut (P-138), 3/16"/4 mm, 5/16-24, Delrin (Acetal), natural

49041039 Upchurch flangeless ferrule (P-133), 3/16", Tefzel (ETFE), blue

Appendix A

A-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

Rep

lace

men

t Par

ts a

nd

Acc

esso

ries

49040681 Upchurch adapter (P-681), 5/16–24 female to 1/4–28 male, 0.125 in thru hole, Kel-F (PTCFE)

49041054 Upchurch P-386 flangeless, headless nut, 1/8", 1/4-28

49041015 Upchurch P-300 flangeless ferrule, 1/8"

49041011 Upchurch P-286 flangeless, headless nut 1/16", 1/4–28

49041021 Upchurch P-200R flangeless ferrule, 1/16"

470504001 PTFE tubing, 0.04" ID x 1/16" OD, natural

470512503 PTFE tubing, 1/8" ID x 3/16" OD

490032 PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot

541180372 Spiral wrap, 3/8" diameter, black

54083751 Retainer, 0.375" diameter, adhesive

54096037 Cable clip, 0.375" diameter

17037116 Stainless steel dispense tube, 2.1" x 0.120", for 1/8" OD tubing

17037117 Stainless steel dispense tube, 2.1" x 0.063", for 1/16" OD tubing

470350006 Tygon drain tubing, 3/8 x 1/2

49081701 Elbow, 1/8 NPT to 3/8 barb

49041032 Upchurch P-297 headless nut extender tool

Tubing and Fittings (Continued)


Cables and I/O Accessories


6730054007 0.5 amp T-0.5 replacement fuse, Slo-Blo

6770100411 Fuse drawer, 220V

6770100412 Fuse drawer, 110V

36078142 Cat 5e ethernet cable

7080316106 Power cord, detachable, 220V

7080318107 Power cord, 18/3, 110V

638314512 Terminal block connector, 14-pin

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide A-3

Appendix AR

eplacem

ent Parts an

d A

ccessories

Miscellaneous


17017103 PREPFC Contact Interface Module to connect up to four fraction collectors

17027114 Cable to connect from PREPFC Contact Interface Module to each fraction collector (one cable required for each fraction collector)

210526 System Organizer, PREPFC

21052609 Pocket PC Guard for HP iPAQ hx2100- or hx2400-Series

2106270Rxx PREPFCTM Control Software

21067530 Gilson Ethernet Utility

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-1

Racks B

The fraction collector can be configured with a variety of rack types and sizes. The following pages describe the racks that can be purchased for use on the fraction collector. Refer to Chapter 2, Installation for rack installation procedures.

Appendix B

B-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

Rac

ks Code 20 rackFor 108 tubes

Material: polypropylene

Vessels and maximum capacity: 10 x 100 mm (4.5 mL)

Part number: 150425

Requires tray insert (part number 17007112)

Code 21 rackFor 60 tubes


Vessels and maximum capacity: 13 x 100 mm (9 mL)

Part number: 150422





18 x 150 mm (32 mL)

Part number: 150424



Appendix BR

acks

Code 22U rackFor 44 tubes


Vessels and maximum capacity: 10–18 mm x 100–180 mm

Part number: 150498


Code 23 rackFor 44 scintillation vials



17 x 65 mm (8 mL)

Part number: 150426


Code 23W rackFor 44 Waters WISP vials



Part number: 270433


Appendix B


Rac

ks Code 24 rackFor 14 scintillation vials



28 x 60 mm (20 mL)

Part number: 150427





10 x 75 mm (3.5 mL)

Part number: 150420





13 x 75 mm (5 mL)

Part number: 150429



Appendix BR

acks

Code 30 rackFor 60 vials

Material: aluminum


Part number: 2704430

Shown installed in thermostating cuvette



Material: aluminum





Material: aluminum


13 x 100 mm (9 mL)


Shown installed in thermostating cuvette


Appendix B


Rac

ks Code 33 rackFor 14 scintillation vials

Material: aluminum




Code 34 rackFor 36 Waters WISP vials

Material: aluminum




Code 37 rackFor 56 vials and one scintillation vial

Material: aluminum

Vessels and maximum capacity: 56 12 x 32 mm (2 mL)

1 28 x 60 mm (20 mL)




Appendix BR

acks

Code 38 rackFor 96 vials and one scintillation vial

Material: aluminum

Vessels and maximum capacity: 96 7 x 41 mm (0.7 mL)

1 28 x 60 mm (20 mL)



Appendix B


Rac


Material: stainless steel








Part number: Requires tray insert (part number 17007112)



Vessels and maximum capacity: 16 x 150 mm tubes


Part number: Requires tray insert (part number 17007112)


Appendix BR

acks

Code 60 rackFor four bottles


Vessels and maximum capacity: 180 mL

250 mL



Code 61 rackFor four bottles



250 mL



Appendix B


Rac

ks Code 200 rackFor 96 Vacutainer tubes

Material: aluminum




Code 202 rackFor 96 Vacutainer tubes

Material: aluminum

Vessels and maximum capacity: 10.25 x 47 mm (2.5 mL)




Material: aluminum

Vessels and maximum capacity: 10.25 x 64 mm (3 mL)




Appendix BR

acks

Code 204 rackFor 27 scintillation vials

Material: aluminum





Material: aluminum





Material: aluminum




Appendix B


Rac


Material: aluminum





Material: aluminum





Material: aluminum





Appendix BR

acks

Code 211 rackFor 9 Boston round screw-cap bottles

Material: aluminum





Material: aluminum


48 13 x 75 mm (4 mL)



Note: Place the shorter tubes into the notched tube locations.

Appendix B


Rac

ks Code 213 rackFor 74 Vacutainer tubes

Material: aluminum


37 16 x 75 mm (10 mL)





Material: aluminum

Vessels and maximum capacity: 48 10.25 x 47 mm (2.5 mL)

48 10.25 x 64 mm (3 mL)





Appendix BR

acks


Material: aluminum





Material: aluminum





Material: aluminum




Appendix B


Rac

ks Code 220 rackFor 70 vials

Material: aluminum




Code 222 rackFor 27 conical bottom tubes

Material: aluminum




Code 223 rackFor 96 tubes with screw caps

Material: aluminum





Appendix BR

acks


Material: aluminum

Vessels and maximum capacity: 8.5 x 41 mm




Material: aluminum





Material: aluminum




Appendix B


Rac


Material: aluminum

Vessels and maximum capacity: 25 x 200 mm




Appendix BR

acks

Code 330For 60 vials

Material: aluminum




Code 333For 14 scintillation vials

Material: aluminum




Code 341For 108 tubes

Material: aluminum




Appendix B


Rac

ks Code 343For 80 tubes

Material: aluminum




Code 345For 44 tubes

Material: aluminum





Appendix BR

acks

Code 91 rackFor 11 Kimax or Pyrex bottles

Material: aluminum



Code 92 rackFor eight Kimax or Pyrex bottles

Material: aluminum



Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide C-1

System Organizer C

Use the instructions on the following pages to assemble the PREPFCTM Fraction Collector System Organizer.

Parts

The PREPFC Fraction Collector System Organizer (part number 210526, ordered separately) includes the following:

Part number Description

21052650 Right side assembly

21052651 Left side assembly

21052601 42" wide shelf, qty 2

21052303T 37" wide shelf stiffener, qty 2

21052602 Corner brace, qty 4

4013192109 10–32 x 5/8" socket cap screws, qty 20

4011141045 6–32 x 1/4" Phillips screws, qty 20

43115621 5/32" ball point Allen wrench

Appendix C

C-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide

Syst

em O

rgan

izer Attaching the Shelf Stiffeners

1 Align the holes on one of the shelf stiffeners with the holes on one of the shelves.

2 Using a Phillips screwdriver and the Phillips screws, attach the shelf stiffener to the shelf.

3 Repeat the process for the second shelf and shelf stiffener.

Attaching the Shelves1 Lay the left side assembly with the beveled (slanted) edge down and the feet

facing you. The color strip faces the left. (If the color strip faces the right in this position, you have the right side assembly.)

2 In the first and sixth hole from the top, insert and slightly finger tighten a socket cap screw on the left nut strip. Then insert and slightly finger tighten a socket cap screw in the same position on the right nut strip.

3 Position the first shelf by sliding its notches between the side assembly and the socket cap screws. Tighten the socket cap screws using the wrench. The shelf will be at a 90° angle to the side assembly.

4 Attach another shelf using the procedure outlined in the previous step.

5 Turn the left side assembly and shelves so the color strip is facing up.

6 To the right of the left side assembly and attached shelves, lay the right side assembly with the beveled edge down and the feet facing you. The color strip faces the right.

7 Insert and slightly finger tighten the socket cap screws in the same locations you attached the shelves to the left side assembly.

8 Turn the right side assembly so the color strip is facing up.

9 Align the socket cap screws on the right side assembly to the shelves attached to the left side assembly. Move the right side assembly until the notches of the shelves slide between the socket cap screws and the right side assembly. Tighten the socket cap screws with the wrench.

10 Lift the system organizer and stand it in its upright position. The front of the system organizer has the two color strips.

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide C-3

Appendix CSystem

Org

anizer

Attaching the Corner Braces1 On the rear of the system organizer, locate the sliding nut strips within the

grooves of the right and left side assemblies and one of the shelves.

2 Insert socket cap screws into the three holes in one of the corner braces.

3 Align the socket cap screws and corner brace with nuts in the sliding nut strips. Slightly finger tighten the socket cap screws.

4 After the corner brace is in place, tighten all three socket cap screws with the wrench.

5 Repeat the process for the other three corner braces.

sliding nut strips

cornerbrace

Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide D-1

Pocket PC Guard D

The Pocket PC Guard for HP iPAQ hx2100- or hx2400-Series is used in conjunction with a laptop computer locking cable to secure the Pocket PC. The laptop computer locking cable must be purchased separately and is not available from Gilson, Inc.

Parts

The Pocket PC Guard for HP iPAQ hx2100/2400-Series (part number 21052609) includes the following:

• Holder

• Cover

• Screws, 4

• 5/64" Allen wrench

Assembly1 Place the Pocket PC in the Pocket PC Guard

holder.

2 Place the Pocket PC Guard cover over the holder.

3 Locate the four screws included with the Pocket PC Guard.

4 Insert the screws in the locations shown in the diagram and tighten using the Allen wrench included with the Pocket PC Guard.

5 Insert the lock end of the laptop computer locking cable into one of the slots near the bottom of the Pocket PC Guard. The cable can be inserted in either slot.

exploded view of Pocket PC Guard

cover

holder

slot

slot

prepfctm fraction collector (ethernet) user’s guide

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