from a laboratory to a factory - ipbo |...
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![Page 1: From a Laboratory to a Factory - IPBO | VIB-UGentipbo.vib-ugent.be/wp-content/uploads/2017/05/... · •Dengue, chikungunya and Zika have overlapping symptoms •Dengue haemorrhagic](https://reader034.vdocuments.net/reader034/viewer/2022042410/5f285133e15c1964ba12f0be/html5/thumbnails/1.jpg)
Page 1© 2017 Oxitec Limited
IIBN Forum, 10th May 2017
Biotechnology: From Innovation to Industrial Production
Amandine, R&D Team Leader
From a Laboratory to a Factory
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Page 2© 2017 Oxitec Limited
What does Oxitec do?
We combat insect-borne diseases and improve crop yields
through the reduction of the insect populations
with a genetic approach that is safe, sustainable, economic and applicable to many insect species worldwide
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Page 3© 2017 Oxitec Limited
Oxitec’s portfolio
Target Crop
MedflyCitrus/pome/
stone fruit
Olivefly Olive
Diamondback Moth
Brassica
Pink Bollworm
Cotton
Target Attacks
Drosophilasuzukii
Soft fruit
Stable flyHuman &livestock
Agriculture
In development
Target Vector of
Aedes aegypti
Yellow fever, Dengue, Zika
& Chikungunya
Aedesalbopictus
Dengue, Zika, &
Chikungunya
Public Health
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Page 4© 2017 Oxitec Limited
Oxitec Ae. aegypti development
2002OX513A created
2009 -Grand Cayman
trial: 96% suppression
2010 -Brazil
Three trials: All >90%
suppression
2014Panama trial +
No Ae. albopictus niche replacement.
No persistence in the environment
2014-2016Brazil: CTNBio Approval, ANVISA
announced they will issue a temporary registration
US: FDA Publish FONSI & EA
Global: WHO PAHO/CARPHA recommend pilot deployment under operational conditions
Piracicaba project (65,000 people)
Cayman project
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Page 5© 2017 Oxitec Limited
Oxitec OX513A Friendly™ mosquitoes
Offspring die before they can reproduce and transmit disease
Oxitec male mosquitoes are produced for release and mate
with wild females
Self-limiting Gene Fluorescent Marker Gene
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Page 6© 2017 Oxitec Limited
Oxitec’s technology
Self-limiting Male-selecting
• Population suppression through ‘genetic sterility’
• Male-only releases• Population suppression via
death of female progeny
Transgenic male insects are released to mate with wild females. Death of progeny leads to population suppression.
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Page 7© 2017 Oxitec Limited
tTAV tTAV works in a positive feedback loop to make more tTAV
Transcriptionalmachinery
tTAV stops the mosquito from expressing essential genes
Pest mosquito offspring die before they can reproduce
In a production environment Tetracycline is added through the diet to block tTAV action and this allows large scale production
Self limiting technology
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Page 8© 2017 Oxitec Limited
tTAV tTAV works in a positive feedback loop to make more tTAV
Transcriptionalmachinery
tTAV stops the mosquito from expressing essential genes
Pest mosquito offspring die before they can reproduce
In a production environment Tetracycline is added through the diet to block tTAV action and this allows large scale production
Self limiting technology
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Page 9© 2017 Oxitec Limited
tTAV tTAV works in a positive feedback loop to make more tTAV
Transcriptionalmachinery
tTAV stops the mosquito from expressing essential genes
Pest mosquito offspring die before they can reproduce
In a production environment Tetracycline is added through the diet to block tTAV action and this allows large scale production
Self limiting technology
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Page 10© 2017 Oxitec Limited
Oxitec’s insects reviewed by independent regulators
Regulatory precedents across all Oxitec GM insects
Oxitec Aedes aegyptireceived approval for outdoor releases in Brazil April 2014
Import and contained trials approved
• Australia• Austria• France• Greece• Guatemala• Mexico
• Israel• Singapore• Thailand• Vietnam• UK
Current applications for outdoor release
• India• Caribbean• USA
Outdoor release approved
• Brazil• Cayman• Malaysia• USA• Panama
FDA Publishes Findings of
No Significant Impact on Oxitec’s Self-limiting
Mosquito
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Page 11© 2017 Oxitec Limited
The growing global threat from Ae. aegypti
• Aedes aegypti vectors dengue, chikungunya and emerging Zikaviruses
• Dengue, chikungunya and Zikahave overlapping symptoms
• Dengue haemorrhagic fever can result in mortality
• Zika virus has been linked to microcephaly and Guillain-Barrésyndrome
• Yellow fever increase with 200,000 cases and 30,000 deaths worldwide annually*
US$39 bn global economic impact
231 cases of Dengue in La reunion in 2016
* Sources: http://www.who.int/ith/updates/20170404/en/
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Page 12© 2017 Oxitec Limited
Invasive mosquitoes in Europe
Ae. aegypti
Ae. albopictusin Europe
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Page 13© 2017 Oxitec Limited
Current vector control
Challenges• Inadequate control• Insecticide resistance• Public concerns about health effects and impacts on the environment and other species
Example of Mosquito Eradication in a School Room
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Page 14© 2017 Oxitec Limited
Oxitec’s OX513A self-limiting strain
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Page 15© 2017 Oxitec Limited
Oxitec’s OX513A self-limiting strain
Site Date
Number of
OX513A males
released (millions)
Grand Cayman 2010 3.3
Itaberaba (Brazil) 2010-2011 17.64
Mandacaru (Brazil) 2011-2012 10.33
Jacobina (Brazil) 2013-2015 70.68
Nuevo Chorrillo
(Panama)2014 4.25
Piracicaba (Brazil) 2015 – ongoing 123.1
Total 229.3
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Page 16© 2017 Oxitec Limited
Beyond a trial: Scaling up production
• Industrialized production
– Efficient, clean processes
– Quality controlledoutputs
• Capacity:
– Space >5,000m2
– 60 million malemosquitoes/week
Scaling up production - Brazil
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Page 17© 2017 Oxitec Limited
Inside our Brazil factoryOpened large-scale facility in Brazil
Capacity of new factory in Piracicaba, Brazil is 60 million Oxitec mosquitoes per week
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Page 18© 2017 Oxitec Limited
Release is planned and adaptive to needsRelease is planned and adaptive
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Page 19© 2017 Oxitec Limited
Oxitec Mosquito Trial Results Summary
Cayman: Mosquito Research Control UnitBrazil: University of São Paulo and MoscamedPanama: The Gorgas Institute
96%
92%
99%
93%
93%
>90%1 suppression of Aedes aegypti in field
trials and projects across Cayman Islands,
Brazil, and Panama
1 Relative to control sites
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Page 20© 2017 Oxitec Limited
Oxitec added strengths
Targeted, cost-effective approach• Males actively seek females• No radiation: No irradiators, lower costs• Demonstrated field effectiveness
Safety• Approved by regulators• Male focused releases • Fluorescent marker: track & trace• Non toxic, non allergenic
Environmentally-friendly strategy • No chemical residues• No adverse impact on non-target species • Not persistent in the environment• Built-in biosecurity
http://beecontrolnw.com/bumble-bees/3307689
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Page 21© 2017 Oxitec Limited
Mosquito Vectors
Ae. aegypti:
Dengue
Chikungunya
Zika
Anopheles species:Malaria
Ae. albopictus:
Dengue
Chikungunya
Zika?
Culex species:
West Nile
Avian malaria
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Page 22© 2017 Oxitec Limited
GM approaches
GM crops Oxitec insects GM -Gene Drive
• Genetic change to food
and feed product
• Genetic change to pest
only
• Genetic change to pest
only
• Unlikely to spread
• Self-limiting • Not self-limiting
• Genes do not pass to
other species• Designed to spread
within a species
• Commercial scale
monitoring and
widespread regulatory
approval
• Simplified monitoring
(fluorescent marker)
• Regulatory approvals
• Need to monitor
carefully
• Uncertain regulatory
path
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Page 23© 2017 Oxitec Limited
Photo credit: Alexandre Carvalho/Oxitec Photo credit: Alexandre Carvalho/Oxitec
Cayman Islands
Support Neutral Oppose
Piracicaba, Brazil
Support Neutral Oppose
United States
Support Oppose
Surveys Show Strong Consumer Support
Monroe County referendum 2016 : 31 of 33 precincts voted in favour of trialling Oxitec’s solution
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Page 24© 2017 Oxitec Limited
Any Questions?
Thank you!