from materials to ice - 4tu.federation · supramolecular polymers particle composition and density...
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Nanoscopy at the interfaceFrom Materials to Ice
Roderick Tas
4TU-workshopUtrecht, October 28th 2019
Voets LabSelf-Organizing Soft Matter
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Nanoscale assemblies in chemistry and soft matter
A) Q. Chen et al., Nature, 2011.B) M.E. Leunissen et al., Nature, 2005.
E) C.M.A. Leenders et al., Chem. Comm., 2013.F) M. Beuwer et al., Pol. Chem., 2016.
Assembly of colloids and colloidal crystals Assembly of supramolecular molecules
A B
C D
E
F
C) W.T.M. Irvine et al., Nat. Mater., 2012.D) A. Yethiraj et al., Nature, 2003.
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Soft Matter microscopy
Zeiss
Soft Materials
In Situ imagingLabeling specificityDynamic information
Super resolution microscopy
20 nm
Electron Microscopy
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Flavours of super-resolution microscopy
Ni et al., 2017 JBioPhotonics
~100nm ~50nm ~10nmLateral resolution
~s ~s ~minTemporal resolution
Single Molecule Localization Microscopy
(SMLM)
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Single molecule localization microscopy
widefield image acquisition reconstructionlocalizations
midpoints200nm
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Lessons from biology
Periodic rings in neuronal neuritesXu et al., 2015 Science
Microtubule orientations dictate selective transport in neuronsTas et al., 2018 Neuron
Spatially resolved single-cell RNA profilingMoffit et al., 2018 Science
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Nanoscopy in material sciences
Albertazzi et al., 2014 Science Conley et al., 2016 CSA
2D micelles characterizationsupramolecular polymers Particle composition and density
Qiu et al., 2016 Science
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• iPAINT: a versatile SMLM tool to study soft matter• Colloidal dispersion in fluid-fluid interfaces• Complex supramolecular polymers
• Resolving antifreeze proteins at the ice-water interface to study their mechanisms
Outline: Applications of SMLM to understand soft matter
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iPAINT
Aloi et al., 2016, Nanoscale
Transient non-covalent adsorption of fluorophores at interfaces
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iPAINT: Colloidal dispersions and interfaces
Aloi, IKV, RSC Nanoscale 2016
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iPAINT: Colloidal dispersions and interfaces
oil
water
iPAINT of a liquid-liquid interface
Aloi, IKV, RSC Nanoscale 2016
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iPAINT: Colloidal dispersions in a fluid interface
Lowers interfacial tension
Reversibly adsorbed
Lowers interfacial tension Forms steric barrier
Irreversibly adsorbed
Surfactants Particles
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iPAINT: Colloidal dispersions in a fluid interface
oil
water
water
oil
Oil phase
Water phase
γso
γow
γsw
θow
Θ > 90°
Θ < 90°
E. C. Giakoumatos et al., In preparation.
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iPAINT: Colloidal dispersions in a fluid interface
Scale bar 500 nmE. C. Giakoumatos et al., In preparation.
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iPAINT: In situ polymer assembly (in organic solvent)
B. Adelizzi,* A. Aloi* et al., ACS Nano, 2018.
WaterProbe exchange
Organic SolventNo exchange
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iPAINT: In situ polymer assembly (in organic solvent)
B. Adelizzi,* A. Aloi* et al., ACS Nano, 2018.
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iPAINTing soft matter
Interfaces Supramolecular systemsColloidal dispersions
1 mm
Aloi, IKV, RSC Nanoscale 2016
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Summary: SMLM to understand soft matter
Complex supramolecular polymersColloidal dispersions in fluid-fluid interfaces
Ice growth inhibition by Antifreeze proteins
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Acknowledgements
Prof. Dr. Ilja VoetsDr. Antonio Aloi iPAINTEmma Giakoumatos Colloidal dispersionsRomà Suris VallsChristian Sproncken
VoetslabLaboratory of Self-Organizing
Soft Matter