KKU. Vet. J. Vol. z No. z July - December 1ee7

? "r s'fl 'r u n r r fi n ur a rifr n r r u o .r frul r nl u r.l r f uI n u ul u ff.r m isrq

55

t'l,,adilauunu gtegao Bovine tuberculin skin test

ltaunlssrtromrtda pathogenic Mycobacterium

'lurfruustuuav nasal secretion

qnlJ't

uirt:y{flnB

qilotu

ru:{n

nqryru'l

ltrmr'U'l'1'l:.1'

fiaaurri'

v{nlttu'

rrrirru:'

n:rnr:lnar'

Vtuilnfl$a

- L:1 .^ i-,.r ,, Y ,q , ;enr:frnurn:'.:fl Lrirfronr.r:^jrjrufifir^lr j:L [nurLq']uruilrn un: usiayr,'lr jlpi''raq1n# 1 nn 6a frdrurirqru

fru rfruvia.: ttn:ilruTuu aru oirl n:irurio eir tno rfio.t c"{uiol?ou uriut'.:fir nrturirlrufrr,rtrr^tril rrir ual, . 9 Y. e ;- , , Irlrjr:lnri :'r:l.rsrfronulu'run:.lli{t::.trnuilcluruhiLlnfnun;urinyylriun".:orjvir.:n:uAa ra:^jfirutusfruaurrfr

tusirlnnialeir:rry*n:lurirrnilruo.: eirrnooun:-nd 6'':r,tiorsou*riut'.reirulr,ldrfiurnuor:n::ruluri

tinn'titterdoll Bov,rne tubercuhn skin test luTnulfitfiurtuairrnotfiosdruru t:g d'rvrlitrnoirtri'zunnr:flfiaolau eirulnurtueirmoalninr-i q-.:nin?ouuriu.iruru tat d'rvw'irvrnd"rfitriplnnr:*rr*^,tiRn'l:tfllsuuntdolurirul6ruo, nurn secretion lutnulfrrdu{"lueirmorfiot.i',r.u ,r, 6'.,r^rlrluuufi-lqt6u'rluairrnolninil"iruru gz oi':r,rt'irnnfr'rlriyrlrda pathogenic Mycobactenum

:'--q,r<qrnnant:frntrrflr,ru-jrqffinr:eo{r-rul:nlur,'lriilnurtrro.ri.:r.riollou uriufrrds.:ururub-tO"ilrfuoit1.1n

nrprlr lnuufr r6u{ luq"lurTorou uriuil'rayrj norr 16 o M. bows ua: M tuber cu]osis

ritdtfrry : :'ru\r-ru irul:nturlrjllnuu

'nrni:urcafiriylur nrut t[?rmun']dni ilfirinurd'slou uriu c.rou*riu 40002

'ntnigrfnrttnmtidror:rudri nru;dnrrrvruunrani rlurinurnlrl?ouuriu c.rlou$riu 40002to':rinr:d.ttailfrqnr:lnuruvi'rrl:;rmnlmu nrnfl;iuoonrfiu{rHfio or.yirn:t a.r0u[riu 40260

56

ollltu'lr-cul: nrlnr rfl uT: n 6 n si os e o rio ;.r vr vnlri'ln

g u fl o r.r a m : r n r : ru d I fi or sl'r u a ; p.r n zu ff rr rih u l n'r n.r

(qry rd u ru, 2527 ) nli n:1e n-nuru:nr uuanfnlrjil{d4 u < - J - - J --ir d"nilh u tilu{ru [:n (tn5 u{ d'nn- unsd'rl dvto, 251 5)

n r : n : r qi fr q d'u r"rul: n1n1n s ilir1il 6'o.: or d'rr n r : n :rcBovine tuberculin skin test oeir.rtdur (tttru;uavnrur, 2525 ; :ru{ruil:;.irflroqjmnutjautnn

rlqd'n'iuvismfr, 2535) uSonr:n:':cairu Bovine

tuberculln skin test nio uri'u nt: n:eq a"nuru :i nr n

s o sI: n u n ; n r : r' : r c n u lda vr r s ri'o.: ri fr rifr n r : vt' l'i-J-^SqlnnrndFl2 (rn:u.:Flnq Ingxud?lfi, 2b1b,drJ']u

llnrnrui 2511 unr 2515)

urinr:vth Bovlne fuberculin skin test

tfi g.r o drr 16arorclri ryr rl.ni alunr:rarqriff nr:?a{irul: nluTn uu rfl :r :q ;lfi'zu nu? n 80-90 uJ oi rtu situ

lniirfluicul:n un;Tnfrrflua1ruT:ndn 1,0-20

rujoirtuaiq;1r.i'e.raaurfic (wipple er a1., 1995) uonL E -qv c Z ;u .a tnqr nu:uur{ n i{ flrIrizu a ur n rfi a vr"t 1 fi d'n'5hi r-le u rfl u

{ruT:n (Jones er a1., 1992) eirunr:n:rq6'ru Bovine

tubercr:lin skin test nia r n1u nr: n:rq ahgru ;i nr a

r o rT: n u a s n r : n : r q y{ u r da vr r.u.{ o .r rj g- rifr n r : q r n.ae) - At

t r n d'n'iq sdr ld'rfi o aTsrdlri'zu n ur n ri o n r:n n d oL B oytne

tuberculin skin test rvitiu lri atn:nn:rctu dn'ifr

tri'n L rfi q ar n nr :?r lryr?u:'r u munr:i{'s do uu n"ryru

'ir arHr:n n:rqfl uTnfr rfl ur-ruI:nlri'ruri r.r n nu rionr:

vlgrdou Bovine tuberculin skin test d']ill:flttUn

nrido M. bovisluvu.:ufrunruh (Neitt er al., 1992):: - r u q ? s i,, n,

u0natnu tlJotfu ltnaglJuoonm luu1uilfl 0.i Lnfl iltu iFl

(rlruia'au ,2527)- Y ^- - z r "n{uunil3?aua{ n.i tni{n1td1t?a0Lnn1:

so.rr-ruI:ntuv{riuTnulfr fr nr: rfi'r.rri nrulrivir'rrTu

rrnfn lunrndtnorfiar $nv eirrnoqrafnri

d'turiorrou uriu lnunr:vro dol Bovine tuberculin

skin test ir r fi'r nr: rnr ; us n ts*o i'ruI: nlu1n u l qr n

uluxJotJ RRy nasal secretion

rr:dr:#oruxrqgaraor{ ars. itfi z arTufi 2 n:ng1ail - #r.nrnru 2s4o

orlnrniuavidnrryirnr:finv{nuroru:su'ix 3-12 fllnudu

6 q t J& ,a v -:lnu tu?{'m ilfl 9l{ au tuc.l142 91fl 0u unufl .11J : -a1fl fl 'llu

lueir rno rfio.r zo v'lriurirnr:meraou Bovine

tuberculin skin test lu1n 141 nrttagrfiuriruufrunu

nasal swab ilrernln 9z n-r druludrmaquainrJ 2z

v{riuvir nr:m I d ou Bovine tuberculin skin test lu1n

t38 d'ruavtfiuirulertri'r nasal swab lrqrnln

1.21. iltriunr:vrncoulu16ou ilqflan1uu 2538

fitnluarsu 2539 TorEJdr Bovine tuberculin skin testd *q ^ -;q6dorrdrlufrt,tri.rfikitnurar.: (caudal fold) airueu

0.1 ua.t'{fiarrurfrdutaruoufrrqurvirnTu 2

ln.eirup'r au ii'.rerndn 7 2 vu. r'{ioilri'unr:vln a audcuoe:muilruilduttnv nasal swab ttn:tatv'l't;ttan

2tfiamrsuo.rrJn'tnn'r:Fr?u 0'r14'ti Lowenstein-

jensen medlum n:rc d'n:J6u;1nTafi udr rfi aTnTnfi iia{d'rlilrsioudrafi Zierrl-Neelson rirrw acid fast

naom tri'riulvrndau niacin test, nitrate test ttn;

pyrazimidase test (Runyon et al., 1980)

rio4 ar'lrillnuufr vired'a rfl uv,lriuiioi'r o qjtu

fruriraru sirr ariru ria oh m o ffi o.t {'.:niEr g ou uriu

B v{ril filnulrf.rrarun 67 d'r fid's 4-13 dh fir'lrilrflumsn:n::rarriraqj 4 v^{ril (ldaturuil::rrru

10 f,) uayrflurnsn:n::rstr,rrioqj + r'{rir (id'a.rY,-

u'rutlJrnu b u), . s ;Y ,q e

Tl1:iltnUilil9l{0U tU U',tUlul]lr0'i 9l',rUd

rirudo ohrnarfios d'.rr,riq?ourriu 7 vlrir frlnuu

vY{ulq 43 il't fld'a 3-9 n"r qnr'{rirrflutnvn:n:q ,3 ?,a

:rulHilrfiu.rururuhitfru 5 iir^lriuTnuuii6'.radlu riruTuunru rirun

rirudo eirmorfios q-{ili;ouuriu s r'{rir fiTn

uryYtulr' 28 d'r fid't.r 3-9 d'r mnv{rittflutnryn:n:, .-v:rairir (rdu{uruil:;lrru 10 "r-l)

rhirTnulfr n"todlu s{rrnurrfr siruarti

qr ;l"r:r ry un;sf'tr nfirunt air moql afnri itr,rin

KKU. Vet. J. Vol. z No. z July - December rggT

rourrriu 22 vhiufiTnulrtrrnln 1,21, ilt fiKu 2-12

ri'r 1our4riu;irulr.roifi unilA 4-s d'rvrnr,lrirrrflu

rnvn:n::rutrarj tr6ialuruhjrfru s -rj) ' -".

AGfl a n'15ilst aa { uav?{t'tt il

Rt:ytfidou Bovrne tuberculm skin testq , . ;& ,c1uy'lri lfi d'.:o d1u air m o ou ainri.iruru 1 4 1 qr"r sa:

luoirnorfror 138 d'r rnr''.l,.i,^*^, ui-n',r,*.,,ruun ir*odrtu uriTnvreir rnaqlaionj"iruru g7 ri'r un;

ail tno rfros 121 a-t 4nor-rlrinl pathogentc

Mycobacteriu- tuvil nasal secrerion un ttudluru

6uuaorrlunr:rsfi I=

i'* , f i: q -n1:Fnulp:.:ul{u? I [r]uilfl[nu{Lu"l{fi?a

rauunurilu[nulnrntluru b-10 fl *#rurizuanr:

nndau Bovlne tuberculin skin test tri'r.rnnlvrnd'r. ,f ;! g -

rr fl rl { ?'t tFl u l yt I a u { tua { fi t n s o u uriu il o u-6 n 1 :? o {!f f 3

fru[:nlnEirurnfio{osn'ir 0.4 % eiruzuanl:rnry*-trguuntfrafru[:n[udruudlua;lu nasal secretion

f 6", J10{lnuiln lil?Iuiflo pathogenic Mycobacteriumj ? ' * "' s o..-?--.-.?- - u o !tt{nl: lil?ru rto?ru tin tuuluilaudSytoufr',:nr:n- [n

u ril a o nr"ruT:n ra3 ohi rfi u frul: n : ; a s fi n ri o dru nr:Y, ! - < o

sr:rqlriilurfior"rul:n1u nasal secretion torTnuuf , * -t , :6eid3l10u [nuuilnan tt0?ru [:n artr,: [:nnu u{nr:

- &*t,fr n r*r n f'r fr lri r.r u a l-fr n r :r o.: r-rul: ntuTn u u ar qfr

d'r rfifla1n nlau ur.JlJrrro o.to:or:rnr: tfluT:n uny

"irurufrErdro.:nr:finur nf{dfr "irurur.io a rfr u1lfi 1d

(ttrrur unyn{ut, 2525 , ury$tuu .2827)

57

A-nr:llrl I nl:?lFldou skin test ttJ5uutfluuniunr:ulntto Mycobacterium lur^lrilTnuttue-snintou*rju

./v

H n R1: tlttl s u untda Mycob acte riumr'{rilrlnuuo f i-

e1u?u Ianil p,l R n']iyt n dau

skin test rfluau u1uilnu nasal secretion

o.ounind"

Q7d/141.")

o. tt.l0't"

021d/138")

hinu'

t41

tJo

lrjyrr' lrinu'

hinu"

" virnr:vrodor,r sion test luTn 44 n"rffoqlu:lu;nuor5nu:lfi1fir.ranr:nnaolnru virnr:n o d on skrn test lu1n r 7 n-rfi oqlu:l u:nu oiquttfilfi'zu nnr:vrn a alal^Y, *' Ulr{utta parhogenic Mycobactenumo

"fluruuilTnfi rfiuun:usnrdoqrn fruldl uny nasal secretion

" .iruru*:"iTnfivirnr:nrrdou Bovine tubercuhn skin test

58 ?1ret1:fro?uilyrrl$r1a{a{ il?," fl{{ z ariufi 2 n:nglail - S'rrerar.r 2s4o

frnfrnrrurkvnrnd5ilq

i'cuT:nTn rfluI:ndrd'ruiiriolri'mornr:aru rf,a nru sid'ss at ou n cu a n n:ailnu u6'.ru{orv!

nrtrrt:r3fra uarorqrfiudun:rrrioqrnrnro.r rouuriu ri'rmirnasai.rtaSilfranr:lnuu drfnsru

rJ:strn:fruiTnnul:ruyrt'rlunq'ruvr'nqnnfinud'n'irfrnd o.: rinr:eit taiufrqnr:lnul ud{rJ:s tvrfllvrEJ R1n

d'.rriuiEnr:ifiqd'rruayd'ruan:I:nfrqnd'o.r il:;nrirr n;iuoontder{tfifio urt,d'nruilvttiqvrid'tr frarltsqe * q

ttnslfi'zuauiluouitnr:n:refirrrolud'n'i irln]u nruru:tri arritru: ttaruru#muflfldau"untu aa

n'l:n:?a Bovine ruberculin skin test t'sqyrflu uoinrlno frlri'nmudrurra6avi'r1ri'':ruddrrScua:

rJ:;Tsrrjsionr:q:onr:fi3odotrsfin'i ua:rirs aeir.rlrtrfrrua

d'nr rfi o n #n'ir-h uo onqln r.l{ #n'j

n:urj o d'sri :ra.rruil:;"irfl r o.rrirr 4u ri a u rvr nrJ q d'n'i ud.rtr fi , zs3 s

rnis'rd'nd oraou unsd'aRmd rr1flrnr*il 2s1.5 n'r:finr*r{ru1:nluTnuilurau'tv,lril rrttar:frnaunnd

s(2):gs-1OZ /

nl?us uJfldrln aqnt afrnruufi Bn ffir::ai uosuaorun fiv{-td 2525 d'n:rnr:rflul:nu:rgaTafraua:

iruT:oluTnuu nr:il::suimnr:soruurivruraurnun:flranini.rii 20 artrd'neuvrnd w6'orrio

ufr:r zg

auru fifior1a 2511 n1:Fr:rqiruT:nlu1nuu #mruv{vrdar: 19(1):13-19

auru frfroqa 2515 fl4r,rrn1:vrodou4ruoifirfiutulnuu #nruvrmdor: 23(1):19-29

ururEjuu rfiu:fi'afr 2827 \za6ndo:rr,r'i'r.r#n'irnrnu fl:nurnfiriu ilr:rdn lria rtto:r uasdr:fiuarni @ r"i'a:rl

' r-ruI:n frufinnr:firuv{ nlttvrnuraluni : urr nq-na

Jone, L., Cox, J., Shepherd, J., Rothal, J., Wood, P. and Radford, A. 1992. Removal of false-positive reaction

from pasma in an enzyme immunoassay for bovine interferon garnma. J.Immnol. Methods.

1,55:233'240.

Neill, S.D", Hanna, J., Mackie, D.P. and Bryson, T.G.D., 1992. Isolation of. Mycobacterium bows from the

respiratory tract of skin test negative cattle. Vet.Rec.J.Br.Vet.Assoc. 131: 45-47.

Runyon, 8.H., Karlson, AG., Kubica" G.P. and Wayne, L.G. 1980. Mycobacterium, p.1,45-178. In Lennette,

EH. Balow, A. Hausler, Jr W. and Tlauant, JP. (ed.) Manual of clinical microbiology, 3rded.

American Society for Microbiology, Washinton, D.C.

Wipple, D.L., Bobn, C.A., Davis, AJ"" JamaEn, J.L. Johnson, D.C. , Nabors, R.S., Payeur, J.B., Saari,

D.A., Wilson, A.J. and WolJ, M.M. 1995. Comparison of the sensitivity of the caudal fold skln

test and a commercial garnrna-interferon assay for diagnosis of bovine tuberculosis. Am.J.Vet.

Res. 56:415-419.

KKU. Vet. J. Vol. z No. 2 July - December 1ee7

Servey Report : An Incident Study of Bovine Tuberculosis

Khon Kaen Province by Bovine Tuberculin skin Test and

Isolation of Pathogenic Mycobacterium in Raw Milkand Nasal Secretion

59

A

in

Thitima

Narisom

Pitak

Rhittichai

Narong

Krisana

Chaital

Na-ngam2

Noimay2

Pilachai3

Wongnen3

Tlakamthail

AbstractThs study selected viUages wluch had many dairy farms and had short distance among

each farm such as da:ry farms tn Ban Sum;ar, Ban Nemthong and Ban Knontan, Tbmbon Bankow,

Arnphour Muang, Khon Kaen Province. Those three vrllages had old and new farms. AIso selected

vtllages wluch had not many dalry farms and long distance among each farms in Tbmbon Na Kum.

Tambon Snsuksumran arid Thmbon Bandong, Amphour Llboruat, Khon Kaen Provlnce. Most of these

were new farms.

One hundred and tMty-eight of darry cattle wluch rarsed in Amphour Muang showed

negattve results to bovine tubercrrln skin test. One hundred and forty-one of dairy cattle in AmphorUbonrat, Khon Kaen Pror,rnce also showed negatrve to that test. The resu-lt from isolatton ofpathogenic lvfycobacterium in raw mtlk and nasai secretion of one hundred and twenty-one of dairycattle ln Amphour Muang and ntnty-seven of darry cattle in Amphour Ubonrat was negative.

From tlus study. the inctdence of Bonne tuberculosis in dairy farms wtuch ralsed more thanfive to ten years was rare case. The dairy farms in Khon Kaen Prolrnce mtght be free formMycobacterium bovis and M. tuberculosjs.

Key words : Servey Report, Bovine TUberculosis

lDept. of Mrcrobiologry, Faculty of MedicLne, Khon Kaen University, Khon Kaen 400022Dept. of Veterinary fublic Health, Faculty of Veterinary MedicLne, IGion Kaen University, Kron Kaen 400023The Northeasten Dairy Promotion Organizatlon, Thapra, Khon Kaen 40000