•Fusion cell gene expression profile

•Molecular mechanism of target gene regulation by Dys::Tgo

•Identify enhancer of dys targets: CG13196, CG15252 and mbo

•Identify dys fusion cell enhancer

dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP

dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys

•Identify dys target gene

•Co-activator or repressor of Dys::Tgo?

LP05454

Exon1 2 3 4 5 6 7 8 9 10

dys 2 (1.7kb)

dys1 (2.7kb)

dys un (3.8kb)

dys intron 2a (3.8kb)

dys intron2b (3.8kb)

Identify dys enhancer

dys1a (1.7kb)

dys1b (1.0kb)

dys intron1 (1.4kb)

dys1b-A (500bp) dys1b-B (500bp)

Enhancer for brain, hindgut and anal pad

dys intron-1 dys intron-2b

dys intron-2a

dys1 fragment contains dys fusion cell enhancer

dys un (S15-17)

dys1 (S12-17)

dys2 ( S15-17)

DT

DT

LT

DB

Leading edge?

DB

DT

LT

S14

S17S16

S14

dys1b (S12-17)

S14

S16

dys1a (S14-17)

S15

S14

S12 S12

dys target gene: CG15252

Exon1

CG15252 con (3.2kb)

9.0 kB 5’ upstream

CG15252 TCGTG (0.8 kb)

TCGTG TCGTG TCGTG

CG15252 enhancer

CG15252 con

CG15252 TCGTGDT

S16

CG15252 enhancer

TCGTG-a 185 bp

TCGTG-b 360 ssbp

TCGTG a+b (270bp) TCGTG c+d (100bp)

TCGTG-c 774 bp

TCGTG-d 792 bp

818bp

TCGTG to GATCCmutation: (waiting for result)

( NO?) (Yes!)

CG15252 enhancer: 100bp with 2 TCGTG sites

DT

DT

S16

S17

CG13196

500bp

350bp

100bp?

CG13196-500 CG13196-350

490bp

Ungoing.....

CG15252 DT expression depends on Sal?

•CG15252-GFP expression in Sal mutant?•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-sal; UAS-dys•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-kni; UAS-dys

Co-activator or repressor of Dys::Tgo?

•Compare enhancer of CG13196 and CG15252, identify potential binding sites (drifter?)