genetics and fruit flies!. i.fruit fly scientific name- drosophila melanogaster a. drosophila-“dew...
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I. Fruit fly scientific name-Drosophila melanogaster
A. Drosophila-“dew lover” (dew-sugar)
B. Melanogaster -“black belly”
C. “Black bellied dew lover”
II. Diptera - 87,000 species of flies, gnats, & mosquitoes
A. Phylum -Arthropoda
B. Class - Insecta
C. Order - Diptera (two winged)
III. Adult fly body has 3 distinct regions
A. Head-sight & smell
B. Thorax attachment w/ 6 legs & 2 wings
C. Abdomen (largest)-digestion, reproduction, & respiration
IV. Can find them …A. Summer time
B. Swarming garbage pails
C. Swarming decaying
fruit
D. Mistaken for
gnats!
B. “Sponging” mouth parts 1. Food must be in liquid form
2. Regurgitate digestive enzymes breaks down food-sponge it up!
C. Ocelli- scores of tiny, 6-sided facets in eyes
1. Can’t see sharp images but goodat detecting movement!
a.Humans see ~
1000 x’s better
E. Antennae - 2 “feelers” head
1. Movable segmented organs-touch, smell, & taste
2. Locate decaying fruit by “smell”a. Alcohol produced
by fermentation
H. Chitin - external skeleton 1. Muscles attached to skeletons on inside2. Do not fatigue easily3. Can fly continuously
=6 hours!4. Can fly ~ 2.5 mph!5. Wings beat
~ 250 times/second!
I. Haltere - club-shaped organ below ea. wing
1. Balancing instruments2. Wing moves up & haltere moves
down
3. If removed, “flies”
in circles!
VI. Food in vials mainly for larvae but adults eat it too!
A. Wheat, oat, soyflour, vitamins, salt,iron, sugar,preservatives, &yeast
B. Food coloring: easierto see larvae/ eggs
VII. Female fly can’t mateuntil ~ 10 - 12 hourspupa form
A. Pick females < 10 hrs old-virgins
B. Once mated female
retains sperm & lays fertile eggs
C. Drosophila egg
1. 0.2 mm long, shiny, white, translucent
2. Outer membrane pattern of hexagonal markings under a microscope
3. Eggs hatch
Breathing apparatus For larva
D. Small, white worm-like larva1. Three/four days eating food2. Stage lasts up to 5 days3. Shed outer covering (cuticle) twice 4. Instar - stage in btwn shedding
E. Larva-ready pupa crawls outof food
1. Outer layer darkens: hard-puparium
2. Worm-like larva changes into adult
a. 3-5 days-process metamorphosis: “change in form”
F. Young fly 1st emerges frompuparium
1. Almost white, abdomen very long
2. Head elongated,wings are wet & crumpled
3. Slowly forms adult features-few hours
4. Small flies do NOT grow into large flies
5. Size = size when emerge from puparium
6. Different sized flies= different species
G. Life cycle
1. Short, lasting 10-15 days, depends on temp
2. 30 generations of flies –studied in 1yr!
VIII. Drosophila -ideal animal forgenetics research
A. Easy to care for B. Small jars /vials containing special
food
C. Easy to tell males /femalesFEMALE MALE
IX. 100’s of genetic variationsin Drosophila
A. Affect body color, shape, pattern of body hairs, eye color & shape, & wing structure
B. Used in genetic studies since 1909
1. Thomas Hunt Morgan made genetic history a. Mated male fly w/white eyes withred eyed femaleb. New flies red eyes (showed
red eyes dominance)
c. F2 gen - 1/4 flies white-eye/malesd.1st genetic trait to be “sex-linked”
C. Discovery - 1 pair chromosomes determines sex 1. Fruit flies 8 chromosomes/body cell2. Morgan found males w/ bent
chromosome3. Called bent chromosome “Y” and the
other one, “X”
4. Therefore, males-XY & females-XX
X. Important commerciallyA. Gov. spent millions to keep pest under
control in CA & FL
B. Mediterranean fruit fly - Med Fly
C. Looks like house fly w/orange/black markings
D. If females are found - destroy crop to keep insect from establishing breeding population
XI. Our Experiment
A. Eye color is sex-linked in fruit flies
1. Red (wild type) dominant over
white (mutant)
B. Carolina Supply company provided P generation
1. White eyed females crossed with red eyed males
2. What is their genotype?
a. R = red eye r = white eye
b. XrXr ♀ XRY ♂3. What flies were
transferred to your vials (the F1 generation?)
Xr
Xr
XR Y
a.1 red eye female: 1 white eye male
XRXr XrY
XRXr XrY
C. Cross two F1 flies to predict your F2 generation
1. XRXr ♀ x XrY ♂
XR
Xr
Xr Y
XRXr XRY
XrXr XrY
2.Phenotypic ratio?
1 Red ♀ (25%) :1 Red ♂ (25%) :1 white ♀ (25%) :1 white ♂ (25%)