glycans are the face of cells - glycotechnica.com · analysis could be done on the led panel. tm...

GLYCANS ARE THE FACE OF CELLS

LIFE DOESN’T EXIST WITHOUT GLYCANS

The glycomics era has arrived!

[email protected] | www.glycotechnica.com

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YOKOHAMA TECHNICAL RESEARCH & PRODUCTION DIVISION

101 Hiranobiru3, 5-28-6, Utsukushigaoka, Aoba-ku, Yokohama, Kanagawa 225-0002 JAPAN TEL : +81-45-913-5803 FAX : +81-45-511-8570

HEADOFFICE


Glycotechnica

The company’s activities are focused on becoming the

global organization for glycan profiling technology to

support human well-being.

Management PrinciplesManagement Term

Harumi TakabatakeChief Executive Officer as PresidentMasao Yamada Executive Director as CSOShoji TsutsumiExecutive Director for Marketing & Sales

C O M P A N Y O U T L I N E

O F F I C E S

Description of Business

• Development, manufacture, and sales of lectin microarrays, software, scanners and necessary devices to perform glycan analysis.

• Development, manufacture, and sales of diagnostic applications

based on glycan analysis for regenerative medicine and biomarker research.

• Export and supply of scientific technology for QC solutions for pharmaceutical industries and generic products.

EstablishedJune 22nd, 2011

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At Glycotechnica, our core values are dedicated to the well-being of society. Our aim is to be a company that supports medical advances. Through a style of management that is built on mutual collaboration between our clients, shareholders and employees, we work towards developing new business opportunities with innovative and enterprising solutions. Focusing on glycan analysis and proteomics-related technology, we work with a number of researchers to develop new technologies which will add value to the pharmaceutical industry. Through this contribution, our purpose is to be a business group that broadens the benefits of pharmaceutical technology to the advancement and well-being of society at large.

Glycotechnica continues this passion and capacity to market its pharmaceutical products as a faithful, established brand which contributes to the advancement of a healthy and prosperous society. Furthermore, building on existing products and the techno-logical knowledge accumulated so far, as well as technologies to be discovered, we will nurture a business that can establish this Japanese-based company as a global leader in the industry.

Glycosylation is one of the most common post-translational modifications of proteins. Almost all proteins are glycosylated. Glycosylation influences changes to folding, targeting, ligand recognition, binding, stability and immunity. The function of the protein is adjusted by glycosylation to suit the respective purpose. Glycans play an important role in cancer, immunology, infectious disease and regenerative medicine. For instance, it is well known that glycoproteins are useful cancer markers. Approximately half of cancer markers utilized in current clinical practice are glycan markers. It is also well known that viruses target glycoproteins on cell membrane receptors.

Glycotechnica’s glycan analysis system is a very useful tool for developing biomarkers, conducting quality control of pharma-ceutical proteins and characterization of stem cells. We aim to improve the future of medical treatment by providing this unique tool to research institutes all over the world.

F I E L DEra for Glycan Research

C O N C E P TOur fellow bioscience friendsChief Executive Officer & President

Harumi Takabatake

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GlycostationTM2200 is an ultra-high speed and highly sensitive optical microarray

scanner utilizing the printciple of evanescent-field fluorescence excitation

to analyze an entire microarray slide in only two minutes.

LecChipTM is arrayed with 45 different lectins which bind to specific glycans. It is possible to measure up to 7 samples in only one chip.

Major differences between GlycoLiteTM2200 and GlycostationTM2200 are:

GlycoStation™

GlycoLite™ 2200

Lectin Microarray: LecChip™

Lectins are well known as glycan binding proteins and can be classified into several categories, for instance: fucose-binders, sialic acid-binders, asialo-form-binders, high mannose-binders and O-glycans binders. We developed a slide glass-like lectin microarray on which 45 different lectins are immobilized in triplicate (LecChipTM). These lectins were selected from a number of candidates, based on the results of the hector-by-hecto project performed at Advanced Institute of Industrial Science and Technology (AIST) in Japan.

The basic idea of our technology is to use these lectin to decipher epitope structures of glycans. However, as is well known, the binding between lectins and glycans is weak so that the washing process easily breaks the bond resulting in loss of the specific binding information. In order to overcome this issue, we developed a dedicated scanner using the principle of evanescent-field fluorescence excitation (GlycoStationTM Reader1200/2200, GlycoLiteTM2200).

In this way, our technology makes it possible to detect even the weakest interactions between lectins and glycans in buffer solutions without any washing process. Glycotechnica is pleased to introduce its novel system GlycoStationTM for quick and accurate glycan structure profiling and analyses. This is a Joint development with AIST.

O U R T E C H N O L O G Y

However, the user interface was improved greatly, so simple analysis could be done on the LED panel.

TM

GlycoStation™ Reader 2200 GlycoLite™ 2200

Fluorescence wavelength Cy3, Cy5, FITC (up to 6) Cy3 monochromatic

Scanning time 10 sec at 1 sec Exposure Time

2 min 35 sec at 1 sec Exposure Time

Lectin

Glycan Washing Process

Lose binding

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SALES / TECHNICAL SUPPORTWe are promoting sales activity not only in Japan but elsewhaere in Asia, America and Europe partic-ipating positively in conferences and exhibitions. Our technical staff support our customers along with our sales staff where installation of our system is required we provide training support for lectin microarray experiments so customers can utilize our products effectively.

A N A L Y S I SGlycan Profiling Analysis Service using Lectin Microarrays (Lectin Arrays)

At Glycotechnica, we firmly believe the era of glycomics has just begun. We also realize glycan profiling is a relatively new and complex science. Therefore it is our strategy to work closely with each and every customer to help comprehend the data being generated. Further to this strategy, we have developed a per sample fee based glycan profiling analysis service, suited to those companies and researchers wanting to begin working with glycan profiling without making a capital commitment. Using our GlycoStation™ and LecChip™ technologies, we will perform glycan profiling analysis on your samples and provide you with a comprehensive analysis and interpretation of the results.

F O R M A T I O NAs a Global Company Specialized for Glycan Analysis Technology

RESEARCH / DEVELOPMENTOur technical staff are specialized in biotechnology, optical equipment, software, and other fields. We are working closely to develop new products with innovative ideas. We are also actively involved in joint research with universities and research institutes.

MARKETINGWe provide tools that can assisit the progress of your research through our product sales and meeting customers’ needs as much as possible. Moreover we are willing to introduce you to not only our products but also other tools from inside and outside Japan.

List of the latest publications related to the company’s technology.

Dr. Hino, Hokkaido Univ. et al. Examined interaction strength between galectins and glycopeptides using GlycoStation.

FDA has published a second paper titled “The Use of Lectin Microarray for Assessing Glycosylation of Therapeutic Proteins”.

FDA has published a paper titled “Glycan Analysis of Therapeutic Proteins”.

AIST has published “Lectin Microarray-based Sero-Biomarker Verification Targeting Aberrant O-linked Glycosylation on MUC1”.

Glycosylation type in pancreatic cancer using lectin microarrays publised by Prof. Miyoshi’s group at Osaka University.

A group at AIST in Japan have published a paper on the effectiveness of Lectin Microarrays to identify phenotypes of advanced gastric cancer.

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A Short-Cut to Diagnostic and Therapeutic Developments

1. DISCOVERY OF BIOMARKERS IN CANCEROUS TISSUES.

Using very small sections of paraffin-embedded tissue, differential glycan profiling has identified potential biomarkers in colon cancer, bile duct cancer, liver cancer, esophageal cancer, breast cancer and uterine cancer.

2. CHARACTERIZATION OF STEM CELLS.

3. FDA PRESENTATION ON ASSESSMENT OF QUALITY CONTROL OF THERAPEUTIC GLYCOPROTEINS USING GLYCAN PROFILES.

4. GLYCOME PROFILING OF THE LIVING CELL SURFACE OF CHO AND LEC1 MUTANT CELLS

A difference in glycome profiles on the cell surface of CHO and Lec1 mutant cells was clearly detected. The cells had been labelled with Cell-Tracker Orange CMRA reagent.

5. GLYCAN PROFILING OF BIOSIMILAR DRUGS E.G. ERYTHROPOIETIN.

6. GLYCAN PROFILING OF INTESTINAL BACTERIA IN RELATION TO INFECTIOUS ABILITY AND IMMUNITY.

7. GLYCOME COMPARISON OF CHO AND LEC1 MUTANT CELL LINES FROM CRUDE SAMPLES.

Characteristic differences in N-glycan binding were observed between CHO and Lec1 cells.

Comparison of Cy3 fluorescence intensities between CHO and Lec1 mutant cells

A Lectin signals higher in CHO

Mal I

PHA(L)

RCA120

PHA(E)

ACG

Lect

ins

CHOLec1

0 0.5 1 1.5 2

B Lectin signals higher in Lec1

PSA

LCA

GNA

HHL

PWM

Calsepa

Lect

ins

CHOLec1

0 0.5 1 1.5 2 2.5 3

Some Examples of Applications

CMRA reagentGlycan

GlycanProtein

Phospholipid

Lectin MicroarrayCell Membrane

Cell Lectin

Living cell glycome profiling

( )

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Glycotechnica’s Glycan Profiling System Features:

Evanescent-field fluorescence excitation measurement in a liquidphase so time-consuming washing is not needed

High sensitivity• 100pg/mL Glycoprotein • 100pM Glycan• 500-1,000 Cells

Easy labelling of glycoproteins • Cy3 labelling onto

glycoproteins

High throughput • Two minutes scanning

Identification of glycan isomers

High versatility and applicability

Applicable for crude samples

Glycan profiling system uses lectin microarrays

Easy preparation

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U.S. PROFILING CENTER SUCH AS WISTER INSTITUTE

Under collaboration arrangement

HEADOFFICE


YOKOHAMA TECHNICAL RESEARCH & PRODUCTION DIVISION


[email protected] | www.glycotechnica.com

Glyco Profiling Service available at BioMedica In Vienna, AUSTRIA

Glyco Profiling Service available In Pensylevania, USA

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glycans are the face of cells - glycotechnica.com · analysis could be done on the led panel. tm...

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