GREEN SYNTHESIS OF SILVER NANOPARTICLES USING PLANT EXTRACT OF Curcuma longa AND ITS ANTIBACTERIAL &

ANTIFUNGAL ACTIVITY

A PROJECT SUBMITTED BY

Mr. SAMUEL KENYANYA.

M. Sc. BT 2nd Year.

UNDER THE SUPERVISION OF

Dr. SURESH KAMBLE.

INTRODUCTION

• Nanoparticles are particles between 1 and 100 nanometers in size. In nanotechnology, a particle is a small object that behaves as a whole unit with respect to it transport and properties.

• Green synthesis is the use of plants, bacteria, and fungi to synthesize nanoparticles. This is done when the metal ions are reduced to corresponding metal Nps.

AIM AND OBJECTIVES

• To synthesize silver nanoparticles by using Curcuma longa and evaluation of its Antibacterial and antifungal activity.

MATERIALS AND METHODS

a. Materials

• A fresh tuber of Curcuma longa.

• Instruments like Autoclave, UV-Visible spectrophotometer, petri plates, and Nichrome wire loop.

• Chemicals like Nutrient agar, ethanol and silver nitrate.

b. methods

1) preparation of plant extract

The sun dried and powdered 0.1 g C. longa tubers is mixed with 20 ml distilled water and kept for 4hrs in room temperature. The C. longa is then mixed with aq. solution of 1mM silver nitrate solution in ratio 1:3 under aseptic condition for 24 hrs at 37oC.

2) characterization of silver nanoparticles:

a. UV-visible spectrophotometer analysis

b. Transmission electron microscopy (TEM)

ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY.

1) Antibacterial activity.

The antimicrobial activity of Ag-NPs was evaluated against a gram-positive and a gram-negative pathogenic bacterium by agar diffusion method. The pathogenic bacteria under test were S. areus and E. coli

2) Antifungal activity.

The antifungal activity of Ag-NPs was also evaluated against fungi C.albicans and A.flavus pathogens as per the agar diffusion method.

RESULTS AND DISCUSSIONS:-

a) Synthesis of silver nanoparticles

The reduction of Ag+ into Silver Nanoparticles during exposure to water extract of C. longa tuber powder was able to be followed by the color change as shown in figure 1.

b) Uv-vis spectrophotometer analysis:

Figure 2 shows the absorbance spectra of reaction mixture containing aqueous solution of 1 mM silver nitrate and extract of C. longa tubers as monitored by using UV-visible spectrophotometry.

Figure 1. A color change to indicate synthesis of Nanoparticles. (a) Clear solution of silver nitrate (b) Initial reaction mixture containing extract of C. longa and 1 mM silver nitrate in 1:3 ratio. (c) Dark brown color change in the reaction mixture containing C. longa extract and 1 mM silver nitrate after 24 hours

c) TEM analysis:

TEM provides a further insight into the morphology and size details of the silver nanoparticles. Figure 5 shows that the AgNPs in the reaction mixture were spherical in shape. The synthesized Ag-NPs using extract of C. longa were found to be in the range of 20-40nm in diameter.

Figure 3. TEM micrograph of AgNPs synthesized using Curcuma longa extract.

Figure2. UV-visible spectrophotometer analysis

of silver nanoparticles synthesized using extract

of C. longa tubers.

ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY:-

When the antibacterial and antifungal activities of silver nanoparticles is checked against two isolates of E.coli & S.aureus, and C.albicans & A.flavus , they show varying clear zone of inhibition.

A clear zone of inhibition against all tested microorganisms was found to be in the range of 09 - 11 mm for the tested bacteria and 10 - 12 mm for tested fungi as showed in figure 4 and 5.

Figure 4. showing S. aureus and E.coli culture plates showing antimicrobial activity of (a)silver nanoparticles, (b) silver nitrate salt, (c) turmeric plant extract and (d) gentamycin

Figure5. showing zones of inhibition

with (a) AgNPs, (b) silver nitrate

solution (c) plant extract and (d)

fluconazole in Candidas albicans and

Aspergillus niger

CONCLUSION

The present study emphasizes the use of plant materials for the green synthesis of silver nanoparticles since this method is clean, nontoxic and ecofriendly. Furthermore, the synthesized nanoparticles have the antibacterial activity against E.coli, and S.aureus as well as antifungal activity against C.albicans and A.flavus.

REFERENCESS:

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