high functionalization of olive leaf extract · olive leaves (containing oleuropein & compound...
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( )
OR
AC
(m
ol-T
rolo
x /
mol
) ORAC
(Oleuropein, )
( )
0 5 10
tR(min)
DPPH: 1,1-Diphenyl-2-picrylhydrazyl
DPPH ( ) �max: 517 nm
DPPH
**: Area under the curve
AUC** ( )
AUC ( )
ORAC
Phenylahistin; PLH
�PLH
( ) TEL : 086-251-8297 E-mail : [email protected]
(Oleuropein, )
( )
0 5 10 tR(min)
OR
AC
(mol
-Tro
lox
/ mol
) ORAC
DPPH: 1,1-Diphenyl-2-picrylhydrazyl
DPPH ( )�max: 517 nm
DPPH
**: Area under the curve
*AUC***( )
AUC( )
ORAC
Oleuropein
Phenylahistin; PLH �PLH
(m
ol-T
rolo
x /
mol
)
DPPH
HPLC
0
2
4
6
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10
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High functionalization of olive leaf extract
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High functionalization of olive leaf extract Hiroshi Kanzaki, Ayaka Takatsu, Teruhiko Nitoda Graduate School of Environmental and Life Science, Okayama University
Ant
ioxi
dant
act
ivity
(m
ol-tr
olox
eq.
/ m
ol)
Ant
ioxi
dant
act
ivity
O
RA
C v
alue
(m
ol-tr
olox
eq.
/ m
ol) DPPH test ORAC test
Microbial conversion of olive leaf extracts results in potent antioxidant compound, which is possibly utilized as cosmetic or food materials.
Olive leaves (containing oleuropein &
compound A)
Baker’s yeast Novel
compound (compound B)
Compound A Substrate
Compound B Product
0 5 10
Reaction with bakers’ yeasts
tR(min)
DPPH: 1,1-Diphenyl-2-picrylhydrazyl
DPPH radical(purple) lmax: 517 nm
Radical scavenging Decrease in absorbance
Antioxidant DPPH test Electron transfer
blank
Addition of Fluorescent
Probe
Addition of Radical initiator
Antioxidant **: Area under the curve
AUC** (blank)
AUC (sample)
ORAC test Hydrogen atom transfer
Enzyme from Streptmyces alblus
Compound B exhibited a pronounced antioxidant activity in both DPPH and ORAC tests.
Phenylahistin; PLH from Aspergillus ustus �PLH
Graduate School of Environmental and Life Science Hiroshi Kanzaki TEL : 086-251-8297 E-mail : [email protected]
ΔPLH prepared by microbial conversion is currently under development as an antineoplastic agent
HPLC chromatogram
Enzyme fromStreptmyces
yalblus
Phenylahistin; PLHfrom Aspergillus ustus
s�PLH
ΔPLH prepared by microbial conversion is currently under development as an antineoplastic agent
Example of high functionalization by bioconversion
Olive leaves (containing oleuropein &
compound A)
Novel compound
)(compound B)(compound B)
nd Aate
0 5 10
CompounSubstra
Compound BProduct
R ti ithReaction with Reaction withbakers’ yeasts
tR(min)
Baker’syeast
HPLC chromatogram
Microbial conversion of olive leaf constituent
Ant
ioxi
dant
act
ivity
(mol
-trol
oxeq
. / m
ol)
Ant
ioxi
dant
act
ivity
OR
AC
valu
e(m
ol-tr
olox
eq./
mol
)DPPH test ORAC test
Microbial conversion of olive leaf extracts results in potent antioxidant compound, which is possibly utilized as cosmetic or food materials.
DPPH: 1,1-Diphenyl-2-picrylhydrazyl
DPPH radical(purple)lmax: 517 nm
Radical scavengingDecrease in absorbance
AntioxidantDPPH test Electron transfer
blank
Addition of Fluorescent
Probe
Addition of Radical initiator
Antioxidant**: Area under the curve
*AUC***(blank)
AUC(sample)
ORAC test Hydrogen atom transfer
Compound B exhibited a pronounced antioxidant activity in both DPPH and ORAC tests.
Antioxidant activity of oleuropein analogs
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2
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6
8
10
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