highly specific and safe probe 1 2 0 · 2010-01-13 · 10mm mouse igg, no mmp1 (n=20) 10mm mouse...
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0
1
2
3
4
5
6
7
0 20 40 60 80 100 120
%ID
/g
Time (min)
Brain
[18F]BF-227
[18F]FACT
0
1
2
3
4
5
6
7
0 20 40 60 80 100 120
%ID
/g
Time (min)
Blood
0
1
2
3
4
5
6
7
8
9
10
0 20 40 60 80 100 120
%ID
/g
Time (min)
Bone
N
O
S
N
NCH3
CH3
O
OH
F18
[18F]FACTFluorinated Amyloid
Imaging Compound of Tohoku University, a
benzoxazole derivative
Channelrhodopsin-2(ChR2)
Channelrhodopsin-fast receiver
(ChRFR)
Channelrhodopsin-wide receiver
(ChRWR)
Channelrhodopsin-1(ChR1)
Expression Absorbance On/off kinetics,
Desensitization
400 480 560 (nm)
A: In vitro autoradiography using [18F]FACT
B: Aβ immunostaining in the same section
C: Microscopic observation of FACT staining
D: Aβ immunostaining in the adjacent section
Enhanced!
Broad!
Reduced!Rapid!
Highly Specific and Safe Probe
for Amyloid β-imaging
Biologically Optimized
Photo-electrical Transducer
More INFO: Available in PC presentation
Welcome: Licensee or Collaboration partner
Contact us: [email protected]
0
20
40
60
80
100
(%)
(a) (b) (c) (d)
3mM PAR1 ab, 2.5nM MMP1 (n=30)
10mM PAR1 ab, 2.5nM MMP1 (n=20)
10mM mouse IgG, no MMP1 (n=20)10mM mouse IgG, 2.5nM MMP1 (n=30)
(a)
(b)
(c)
(d)
PAR1 antibody decreased the invasion of cells to about 40%.
(a) (b) (c) (d)
2
46
8
10
12
14
16
18
(mm
/h)
PAR1 antibody decreased the migration of cells to about 50%.
100%
54%
KPL-4 (no MMP1)(a)
PAR1-expressing KPL-4 (10mM mouse IgG, no MMP1)(b)
PAR1-expressing KPL-4 (10mM mouse IgG, 2.5nM MMP1)(c)
(d) PAR1-expressing KPL-4 (10mM PAR1 ab, 2.5nM MMP1)
Anti-PAR1 Antibody
Promising for Cancer Therapy
More INFO: Available in PC presentation
Welcome: Licensee or Collaboration partner
Contact us: [email protected]
Negative-DEP makes
Easy and Rapid Cell Patterning
10kHz, 20Vp-p1MHz, 20Vp-p
Positive DEP Negative DEP
100mm 100mm
Cells
0 sec 0.12 sec 0.24 sec 0.36 sec
Rapid patterning
Device based on n-DEP
cultivation
template IDA electrode
reuse
culture slide
30mm
Patterning of two types of cell
remove excess cells patterning with a second cell type
patterning with a first cell typeinjection of cells in the device
New Resource ‘‘CW-CMS’’ for
Hybrid Rice BreedingHybrid Rice Technology
S
S
S
S
S
rfrf rfrf
rfrf
rfrf
RfRf
RfRf
rfRf
CMS line
CMS line
Maintainer line
No Rf genes
Pollen Pollen
CMS line Restore line
(with Rf gene)
cytoplasm
nucleusSeeds for our food
F1 HybridsHybrid Vigor
High yields
Seed Company
Farmers
Unique Feature of Restore gene ‘‘Rf17’’
Reduced
expression
A→T
Mutation in
promoter
No perception of retrograde signals
from mitochondria
Existence of restore gene is
quickly detectable by PCR-RFLP
More INFO: Available in PC presentation
Welcome: Licensee or Collaboration partner
Contact us: [email protected]
Build up Antibody with High
Affinity for ZnO Surface
Antibody molecule: Camel type
ZnO binding peptide:
EAHVMHKVAPRP
against ZnO
CoO
Al2O3
: Kd = 9 nM
: Kd = 124 nM
: Kd = 54 nM
+
Anti-ZnO
Anti-Au
+Anti-GFP
Anti-ZnO/Au
Anti-ZnO/GFP
抗体濃度 [mM]
Au
粒子
残存
率[%
]
抗体濃度 [mM]
Au
粒子
残存
率[%
]A
mo
un
t o
f A
u p
art
icle
s
in S
up
ern
ata
nt
/ [%
]
Genetic Construction of Bispecific Antibody
Antibody concentration [mM]
ZnO plate
GFP
For flow type biosensor
Property of Anti-ZnO/Au Bispecific Antibody
Application of Anti-ZnO/GFP
Bispecific Antibody
300 350 400
250
300
350
Fl. Int. for APMB-C/ATMND
Fl.
In
t. f
or
AP
MB
-G/A
TM
ND
Noncovalently Fluorolabeled
Probe for Genotyping
Hydrogen bonding
… … …… … …
………
AP site-containing molecular beacon (APMB)
Ligand for APMB
400 450 500 5500
50
100
150
200
N N NH2
AMND
Kd = 375 nM
fl = 0.38*
N N NH2
AND
Kd = 3625 nM
fl = 0.28*
N N NH2
ADMND
Kd = 171 nM
fl = 0.50*Fl. Int.
/ a
. u.
N N NH2
ATMND
Kd = 57 nM
fl = 0.54*
Analysis using APMB/ATMND
APMB + ATMND
Fluorescence:
QUANCHED
+Target DNA
(PCR product) Fluorescence:
ENHANCED
Hybridization
Ex. K-ras gene mutation
Target sequence:
5’-GTTGGAGCTGNTGGCGTA GG- 3’
GGT (wild, Gly)
GCT (mutant, Ala)
G/G
G/CC/C
More INFO: Available in PC presentation
Welcome: Licensee or Collaboration partner
Contact us: [email protected]
Semiconductor Laser Optical-
Pulse Sources for Bioimaging
Pulse Generator(~100 pico second interval)
(~100 MHz repetition rate)
Semiconductor Laser(1.55 mm of wavelength)
Optical
Filter
Nonlinear-Optic Wavelength
Converting Device
Low Noise Optical
Pre-Amplifier
Optical
Filter
Low Nonlinear effects
Optical main-Amplifier
Supercontinuum Light
Photonic Crystal
Fiber (PCF)
Promising for optical source of laser microscopy to
obtain high resolution fluorescence bioimages