house fly research...

House Fly Research Journal May 6, 2010 (C.M.)

-Fly Larvae (100) arrive ~ 4:30pm

-Ordered house flies from Carolina Biological

May 7, 2010 (C.M.)

-Took 12 glass vials from media kitchen

- Into each vial: – put strips (~1” x ~3”) of paper towel into the bottom ~1” deep

– put ~3ml of reconstituted powdered skim milk

–put another ½” deep of paper towel strips on top of it

-Summary: Each vial had some milk, saturated paper towels, with some dry paper towels above.

-Placed each vial in a jar and placed jars in the greenhouse

May 10, 2010; 4:06 pm (B.M., J.M., C.M.)

-Measured larvae and replaced vials in jars

-Replaced foam stopper in vials with larvae; did not replace foam stopper in those without larvae

-Poked holes in jar lids

May 11, 2010; 4:00pm (J.M., B.M., S.H.)

-Counted larvae in vials

-Did not go through vials that contained all pupae yesterday

-Went through all vials with missing/dead larvae from yesterday and replaced/found them

May 12, 2010

-Did not count/measure anything since all were pupae

May 13, 2010

-Did not count/measure anything since all were still pupae

May 14, 2010

9:00am (C.M.)

-Noticed adults in three jars

-Prepared food (1:1 sugar: powdered milk)

-Put ~1” mixture in the bottom of tubes

-Prepared water and paper towels

-Put ~1” of material in the bottom of tubes

4:00pm (B.M., J.M., C.M.)

-Counted adults

-Put food & water tubes in place

-Jar 2: -Found one emerging fly in larval food tube (may or may not be alive)

May 15, 2010; 1:45pm (C.M.)

-Put remaining pupa from Carolina in container with food and water to breed extra adults for colony

-Jar 2: -Found one dead fly

May 16, 2010; 1:45pm (C.M.)

-~18 flies in “extra” jar/colony emerged

May 17, 2010; 4:30pm (B.M., S.H., C.M.)

-Counted number of flies and checked for eggs

-No eggs spotted

May 18, 2010; 2:40pm (B.M., S.H.)


-No eggs spotted

-Jar 8: -Discovered one dead fly

May 19, 2010; 4:30pm (B.M., S.H., H.B.)


-No eggs spotted

May 20, 2010; 3:00pm (B.M., S.H.)


-No eggs spotted

-Jar 8: -Observed one fly in tube possibly laying eggs

May 21, 2010; 2:30pm (B.M., S.H.)


-No eggs spotted

-Jar 6: -Could not locate third fly (possibly dead?)

May 24, 2010; 1:00pm


-Jar 10: -Observed one fly in tube (possibly dead or laying eggs?)

-Found eggs

-Know that breeding and laying eggs is possible with current setup

-Jar 8: -Found another dead fly


-Jar 5: -Larvae found

May 25, 2010; 11:00am

-Put soaked dehydrated liver in bucket and pickle jar for adult flies to lay their eggs on

-Several adults escaped into the greenhouse in the process

May 26, 2010; 11:15am (C.M. & H.B.)

-Counted flies


-Jar 10: -Couldn’t find one fly

May 27, 2010; 11:00am (C.M., N.M.)

-Counted flies

-Jar 10: - eggs still not hatched


-Discovered more larvae

May 28, 2010; 11:45am (C.M.)

-Jar 10: -Found one larva (looks dead)

-Jar 11: -Found one larva

-Could not find any visible eggs

June 1, 2010; 12:35pm (N.M.)

-Jar 3: -Found ~10 larvae and ~12 pupae

-Jar 6: -Found one larvae and three pupae


-Jar 10: - Took eggs out to look at and lost some flies in the process

-Jar 11: -Found two dead flies

-Counted ~33 larvae

(N.M., B.M., H.B.)

-Started 1st generation of incubator jars

-New Brunswick Excella E25 Incubator Shaker Series

-33.5 o C

-Setup:

-Jar 1: -12 eggs with deflated eggs and woodchips

-Jar 2: -16 eggs with woodchips

June 2, 2010; 8:30am (B.M.)

-Checked jars in incubator for larvae

-Recorded incubator temperature at 30.9o C

-Did not find any larvae

-Checked for eggs on liver

-Observed several eggs to use in next jars

-Set up new jars:

-Jar 3: -16 eggs

-Put in first incubator (31.7o C)

-Removed lamp and placed on lid over glass

-Hoping to stay around 30o C

-Jar4: -16 eggs

-Left out at room temperature with lamp

-Jar 5: -19 eggs


-Jar 6: -15 eggs




-Jar 9: -14 eggs and some deflated eggs with woodchips

3:30pm (B.M., H.B., N.M.)

-Went to Virginia Croskery’s farm and collected first set of fly traps (placed May 26)

-Each of three fields has three flytraps and one temperature logger

-Placed traps from each field in a freezer bag and into the freezer to remain frozen until collection

-Final setup:

-Jars 1, 2, 3: -Placed in New Brunswick Scientific Excella E25

-Temperature: 30oC

-Light source: -Placed outside on lid

-Set to timer (7:00am-8:30pm)

-Jars 4, 5, 6: -Placed on incubator

-Temperature: Room temperature ~22oC

-Light Source: -Used lamp set to same timer

-Jars 7, 8, 9: -Placed in Revco Variable Temperature Incubator (Room 139)

-Temperature: 15oC

June 3, 2010; 10:00 am (B.M., H.B.)

-Jar 2: Measured three larvae

Spilled liquid from the jar along with one egg

Replaced the egg in the jar

-Jar 3: Found three larvae (too small to extract and measure)

Killed one larva while trying to extract it

-Observed that jars at higher temperatures had more liquid

Might be a potential problem for drowning eggs and larvae

2:30pm (N.M.)

-Jar 2: Found three larvae

-Jar 3: Didn’t see any larvae

-Jar 1, 7, 8, 9: No activity spotted

June 4, 2010; 9:00am (B.M.)

-Jar 1: No larvae spotted

Observed mold starting to form

-Jar 2: No larvae visible due to formation of mold

-Jar 3: No larvae

No mold (perhaps due to tipped over vial and higher milk level in vial)

-Jar 4-9: No activity visible

11:30am (N.M., H.B.)

-No larvae spotted in any jars

Possibly drowned from milk mixture (Need to put less in)

-Jar 3’: New jar placed into 30oC incubator

Contained 18 eggs

Tried new set up

Used small Petri dish containing moist woodchips and powdered milk

Tried to alleviate drowning and improve accessibility to larvae/pupae

June 5, 2010; 10:45am (B.M.)

-Jar 3: Measured one larva

-Jar 3’: No visible activity spotted


May have killed one egg in the process of measuring

June 6, 2010; 7:30pm (N.M.)


-Jar 3’: No activity seen

Observed Petri dish to be very dry (needs more liquid food)

-Observed mold in all other jars

June 7, 2010; 1:15pm (J.M., B.M., S.H., H.B.)

-Jar 2: Two pupae measured

-Jar 3: Three larvae measured

June 8, 2010; 8:50am (J.M., B.M., S.H., H.B.)

-Jar 2: Same as yesterday

-Jar 3: Two pupae and four larvae measured

One of larvae appeared to be dead

1:30pm (J.M., B.M., S.H.)

-Changed water and adult food in big bucket in greenhouse

-Punched more holes in the jar lids

-Added adult food and paper towels with water to the jars

-Jar2: Same as yesterday

-Jar 3: confirmed one larva to be dead

Three larvae measured

Two pupae (same as yesterday)

June 9, 2010; 8:45am (J.M., S.H.)


-Jar3: Measured four pupae

Measured one larva (possibly dead)

1:46pm (J.M. & S.H.)

-Jar 2: Same as morning

-Jar 3: Same number of pupae as morning

Confirmed death of one larva

-Went to Irish Runs with Heidi

Collected/Replaced sticky traps

Stored collected traps in Room 323 freezer

June 10, 2010; 9:00am (J.M. & S.H.)


-Jar 3: Measured five pupae (larva was not dead yesterday)

-Emptied 12 old jars into big bucket in greenhouse

Cleaned out the vials and jars

Found several larvae and pupae which were put in the big bucket as well

-Replaced wood chips soaked with milk solution in big bucket

-Put rehydrated liver with woodchips into the big bucket to collect eggs

2:40pm (J.M. & S.H.)

-All jars same as morning

-Dug through room temperature jars

-Jar 5: One larva measured

June 11, 2010; 9:00am (J.M. & S.H.)

-Jar 2: Found one fly

One pupa remains



1:00pm (J.M. & S.H.)

-Jar 2: Found two adult flies

-Jar 3: Saw four pupae

-Jar 5: Same as earlier

1:40pm (J.M. & S.H.)

-Retrieved eggs from woodchip dish

-New setup:

Put paper towels with milk solution in small Petri dish with woodchips containing eggs

Left Petri dish lids on

Placed Petri dishes in jelly jars with multiple holes in jar lids

-New jars at 30oC:

Jar 10: Approximately 33 eggs


-New jars at 15oC:



-New jars at room temperature:




-Took jars 3’ and 1 out of incubator (moved temperature logger to jar 11)

June 12, 2010; 3:36 pm (J.M. & B.M.)

-Jar 2: Found two adult flies

-Jar 3: Contained one adult fly and three pupae

-Jar 5: Measured one pupa

-Observed no changes in new jars

June 13, 2010; 2:14pm (J.M. & B.M.)


-Jar 3: Contained four adult flies


-Jar 16: Observed that eggs look discolored (brown in color)

9:28pm (J.M. & B.M.)

-Jar 2: Same as afternoon



-Jar 10: Observed that some of eggs were discolored

-Jar 11: Measured two larvae

-Jar 16: Eggs are discolored

-Took lids off of all Petri dishes

10:00pm (J.M., B.M., S.H.)

-Found more eggs in the big bucket

-Set up new jars and placed at room temperature:




June 14, 2010; 8:45am (N.M.)

-Jar 2: Contained two flies still

-Jar 3: Contained four flies still


-Jar 5: Contained one pupa still

1:15pm (J.M. & S.H.)

-Jar 2, 3, 5: Same as morning

-Jar 11: Three larvae measured

One of larvae is dead

-Added more milk to Petri dishes

-Searched through 15oC jars with forceps and didn’t find anything

June 15, 2010; 9:00am (J.M. & S.H.)


-Jar 3: Found one dead fly and three living flies


-Jar 11: Measured four larvae, one of which was dead

1:00pm (N.M.)




-Jar 10: Eggs appeared dried out, added more milk


June 16, 2010; 9:00am (J.M. & B.M.)





-Jar 16: Observed a small amount of mold starting to form

-Jar 17: Noticed ~15 larvae (too small to measure)

-Jar 18: Observed a small amount of mold starting to form

-Added more milk to Petri dishes to keep them moist

June 17, 2010; 9:00 am (H.B., J.M., B.M.)

-Added more milk to 15oC Petri dishes

-Jar 2: Same

-Jar 3: Same

-Jar 5: Same

-Jar 11: Measured one larva (appeared to be very dry)

Couldn’t find other larvae

-Jar 16: Observed more mold

-Jar 17: Measured 67 larvae

Mold observed

-Jar 18: More mold growth observed


1:10pm (J.M. & B.M.)

-Checked 30oC jars for changes and added more milk

-Jar 2 & 3: Same


6:30pm (N.M.)

-Jar 5: Removed some mold

-Jar 14: Observed that not many eggs remained

-Jar 16: Noticed very few eggs

June 18, 2010; 9:00am (J.M., B.M., H.B.)

-Jar 2: Same

-Jar 3: Found one dead fly, two living

-Jar 5: Same

-Jar 11: Measured two pupae

Found one dead larva




-Jar 19: Measured five larvae


2:20pm (N.M.)

-Jar 2: Contained two flies, two pupae, and many larvae

Removed adults, larvae, and pupae and placed in big bucket to regenerate colony

-Jar 3: Same

-Jar 11: Same

-Added milk to Jars 14, 15, 18, 19

June 19, 2010; 1:30pm (N.M.)

-Added milk to 15oC jars

-Jar 5: Same

-Jar 11: Same




-Jar 19: Couldn’t find anything

Noticed a lot of mold

-Added milk to big bucket in greenhouse

June 20, 2010; 4:00pm (H.B.)

-Counted in 15oC room, found nothing

-Mixed new milk

-Jar 5: Same

-Jar 6: No activity spotted

-Jar 10: Removed some mold and added milk

-Jar 11: Same two pupae

-Jar Blank: Two flies


Removed some mold


-Jar 16: Removed some mold

-Jar 17: Measured 60 larvae (two of which may be dead)

Washed out 12 dead larvae that were dried to side of jar

-Jar 18: Measured 25 larvae (one of which may be dead)

-Jar 19: Measured six larvae (one of which may be dead)

Removed some mold and added milk

June 21, 2010; 10:55am (J.M., B.M., H.B.)


Found two dead larvae


Found six dead larvae

-Jar 19: Measured six larvae

-Cleaned out ALL jars except jars 17, 18, and 19 (room temperature jars)

-Autoclaved jars

-Placed new woodchips with rehydrated liver and milk in big bucket to collect new eggs

3:15pm (J.M. & B.M.)

-Set up new jars (left Petri dish lids on)

-Jars at 30oC:

Jar 20: Between 32 and 35 eggs




-Jars at 15oC:



Jar 26: Approximately 50-60 eggs (some possibly lost during transfer)

-Moved jars to far side of 30oC incubator to avoid direct air circulation and drying out

-Added 600ml of water in a beaker to 30oC incubator for humidity purposes

-Added pan of water to bottom of 15oC incubator for humidity purposes

-Confirmed one fly retrieved on June 9 from field three sticky trap

-Confirmed four flies retrieved on June 16 from field three sticky trap

8:05pm (N.M.)

-Observed all jars in 15oC jars to be well hydrated

-Added drops of water to jars 20, 21, 22, 23 in 30oC incubator

June 22, 2010; 9:00am (J.M. & B.M.)

-Saw some small larvae in new 30oC jars (too small to measure)

10:50am (N.M.)


Found four dead larvae

Cleaned out jar with water and added some milk



-Added water where needed

-Didn’t see any larvae in new jars (didn’t want to poke around and endanger them)

3:00pm (J.M. & B.M.)

-Jar 17: Returned larvae to Petri dish

-Jar 18: Returned all larvae at top of jar to bottom of jar

-Jar 19: Found one larvae sticking out of lid hole

Returned it to inside of jar

-Jar 20: No activity spotted (very wet, perhaps drowned)

-Jar 21: Two small larvae visible

-Jar 22: Found one small larvae on bottom of jar (too small to get back into Petri dish)

-Jar 23: -Saw one small larva (too small to measure)

-Noticed two pupae or pupae casings in food vial in old jar

-Determined that too much water was being added to bottom of jars

June 23, 2010; 9:20am (H.B., J.M., B.M.)

-Jar 17: Measured 35 larvae (one of which is possibly dead)




-Jar 20: Spotted two live larvae

Found three dead larvae

-Jar 21: Spotted ~two larvae (too small to measure)

-Noticed eggs in jars 24 and 25 were drying out (added more water)

-Found pupa on counter, searched for more, put pupa in Jar 7 with adult food and water

3:00pm (H.B., B.M., J.M.)

-Looked at 15oC and 30

oC Petri dishes using microscope

-Jar 20: Noticed some bad eggs and some good eggs

-Jar 21: Observed eggs look good

Noticed at least one larvae (very small)

-Jar 22: Observed that eggs looked rather dead

-Jar 23: Noticed some bad eggs and some good eggs

-Jar 24: Spotted ~nine larvae with microscope

-Jar 25: Spotted ~three larvae

Eggs appeared deflated or hatched?

-Jar 26: Spotted no activity

-Rehydrated jars 17, 18, & 19

-Searched for more larvae in lab, found three more pupae and three larvae in pipette box

June 24, 2010; 9:00am (J.M., B.M., S.H.)



-Jar 18: Measured six larvae (two of which appear to be dead or pupating)

-Jar 19: Measured five larvae

Measured one pupa

-Jar 20: Nothing spotted, no mold growth

-Jar 21: Spotted two small larvae, no mold growth

-Jar 22: Nothing spotted, no mold growth

-Jar 23: Nothing spotted, a lot of mold

-Jar 24: Observed that eggs looks hatched using microscope, no larvae spotted

-Jar 25: Eggs looked good, no larvae spotted

-Jar 26: Eggs looked fine, no larvae spotted

-Took lids off of Petri dishes in all environments

4:00pm (N.M.)

-Jar 17: Appeared dry, added water

-Jar 18: Confirmed two dead larvae

-Added water to all 30oC jars

June 25, 2010; 10:00am (J.M., S.H., B.M.)

-Found one pupa on counter, put in jar with others (five total pupae)

12:35pm (N.M.)


Measured three pupae

Found five dead larvae

Rehydrated with milk and water

-Jar 18: Measured two larvae (one of which was possibly dead)

Measured two pupae

-Jar 19: Measured four larvae

Measured two pupae

-Jar 21: Covered eggs to give moisture

-Noticed that all jars are still moist

June 26, 2010; 11:30pm (B.M.)

-Jar 23: Noticed a lot of new (black) mold on paper towels

June 27, 2010; 4:00pm (N.M.)

-Jar 17: Found eight pupae


Measured 24 larvae

-Jar 18: Found three pupae

Measured one larva

-Jar 19: Found two pupae


Measured two larvae

-Came to the conclusion that the eggs are most likely dead since they haven’t hatched yet.

June 28, 2010; 9:00am (B.M., J.M., S.H., H.B.)

-Jar 7: Contained five (found) pupae

-Jar 17: Found one dead larva

Measured 11 pupae

Measured 12 larvae

-Jar 18: Found three pupae

Measured one larva

-Jar 19: Measured three pupae

Measured one larva



-Jar 22: Searched through with forceps and found nothing



-Jar 25: Found one larva

-Jar 26: Looked under microscope and noticed that eggs still look healthy

-Scraped mold out of 15oC and 30

oC jars

-Decided to only count the larvae and pupae ( In hopes of improving their survival rate by not measuring and

handling them as much)

4:00pm (B.M., S.H.)

-Rehydrated each jar

-Noticed no changes since morning

June 29, 2010; 9:15am (S.H., J.M., B.M., H.B.)

-Jar 17: Found nine larvae, one dead larvae, and 16 pupae

-Jar 18: Found four pupae

-Jar 19: Found four pupae

-Jar 20: Found one pupa

-Jar 21: Found one larva and one pupa

-Jar 23: Found one larva and one dead larva

-Jar 25: Found one small larva

Scraped out mold

-Decided to begin adding 2ml of milk to every jar each morning and 2ml of water to every jar each afternoon

12:45pm (N.M.)

-Rehydrated each jar with 2ml of water and added water to pan in 15oC incubator

-Jar 17: Noticed no changes since morning

-Jar 18: Noticed no changes since morning

-Jar 19: Noticed four pupae and removed one dead larva

June 30, 2010; 9:00am (S.H., J.M., B.M., H.B.)

-Jar 17: Found 15 pupae, two pupating larvae, and two larvae that might be pupating or dead

Found six dead larvae and one living larva




-Jar 21: Found two pupae

-Jar 23: Found one dead larva

-Jar 24: Searched through jar with forceps and found nothing

-Jar 25: Searched through with forceps and found the same small larva which might be dead


-Blank jar: Contained two flies

-Added adult food (approx. 10-20ml) and water to jars containing pupae

-Took off mesh netting in jars containing only pupae

-Decided to add 3ml milk in the mornings and 3ml water in the afternoon since jars were still drying out

3:50pm (N.M.)

-Hydrated all jars

July 1, 2010; 9:20am (J.M., S.H., B.M., H.B.)

-Hydrated all jars

-Jar 17: Found one dead larva, 15 pupae, two pupating larvae

Confirmed two dead pupae





-Jar 24: Found nothing

-Jar 25: Found one small larva, might be dead

-Jar 26: Found nothing

2:30pm (N.M.)

-Hydrated all jars

-Only added 1ml to jar 19, which looked over-hydrated

July 2, 2010; 10:30am (N.M.)

-Jar 17: Found 17 pupae

-Jar 18: Same

-Jar 19: Same

-Jar 20: Same

-Jar 21: Same

-Jar 24: Didn’t see anything



-Blank Jar: Contained two flies

-Added water to colony in greenhouse

-Hydrated other jars with 3ml of milk

2:30pm (N.M.)

-Added 3ml water to each jar

July 3, 2010; 12:15pm (B.M.)

-Jar 7: Contained three flies and two pupae

-Jar 17: Appeared to be over-hydrated, did not add water



-All other jars appeared to be the same as yesterday

-Hydrated all other jars with 3ml water

8:00pm (B.M.)

-Jar 21: Noticed one fly and one pupa

-Hydrated all jars with 3ml water

July 4, 2010; 10:30am (B.M.)

-Blank jar: Contained two flies, one dead fly in water tube

-Jar 7: Contained three flies and two pupae

-Jar 17: Contained 17 pupae

Added 3ml water

-Jar 18: Contained four pupae

Appeared to be over-hydrated


Appeared to be over-hydrated

-Jar 20: Contained one fly

-Jar 21: Contained two flies

-Jar 24: Did not see anything

Added 3ml of milk


Added 3ml of milk


Added 3ml of milk

-Recorded 15oC humidity measurement of 34%

-Recorded 30oC humidity measurement of 43%

7:00pm (B.M.)

-Added 3ml of water to jars 24, 25, & 26

-Noticed that jars 17, 18, & 19 appeared to be over-hydrated

July 5, 2010; 11:00am (H.B.)

-Jar 17: Noticed that the jar appeared to be wet, but not as bad as jars 18 & 19

-Jar 18: Noticed that the jar was very wet and emptied some water from the bottom of the jar

-Jar 19: Noticed that the jar was very wet and emptied some water from the bottom of the jar

-All other jars appeared to be the same as yesterday

-Emptied jars 24, 25, & 26

-Noticed one loose fly in lab 119 and two flies in the netting around the box of pipettes

July 6, 2010; 9:00am (H.B., S.H.)

-Changed out feminine pad in greenhouse colony

-Put wheat bran/calf manna mixture in place of woodchips in greenhouse colony

-Placed soaked liver on top of wheat bran/calf manna mixture to collect eggs

2:30pm (B.M., J.M., S.H.)

-Received fly cages today in mail

-All jars appeared to be the same as yesterday

-Emptied pipette net into fly cage (Cage1)

-Also added jar 7 into Cage 1 (now contains 7 flies)

4:45pm (B.M. & J.M.)

-Collected eggs from the wheat bran/ calf manna mixture

-Made 12 new jars

-Each jar contained a Dixie cup with 60-65ml of wheat bran/calf manna mixture and 35-40ml of water

-Jars at 35oC (changed 15

oC incubator to 35

oC) :

Jar 28: Contained approximately 38 eggs






-Jars at Room temperature:







July 7, 2010; 9:00am (H.B., J.M., S.H., B.M.)

-Noticed all jars to contain the same amount of pupae/flies as yesterday

Added 5ml water to each Dixie cups at room temperature

Noticed some of the eggs at room temperature to be hatched or deflated

-Cage 1: Contained six live flies and two dead flies (accidentally killed through transfer)

Moved cage 1 to greenhouse for observation

-Discovered temperature failure in 35oC incubator: incubator read 47.4

oC, surpassing the 38

oC threshold

Used independent thermometer to confirm high temperature

Emptied jars 28-33 into one jar to be kept in the greenhouse for observation

-Emptied greenhouse colony into a new fly cage (Cage 2)

Added more rehydrated liver to pad to collect eggs

1:30pm (H.B., J.M., S.H., B.M.)

-Noticed jars 34-39 to be moist, did not add more water

-Added wheat bran/calf manna mixture to cage 1 in greenhouse

July 8, 2010; 10:00am (J.M., B.M., S.H.)

-Did not find any eggs in greenhouse cages

-Noted that the incubator in room 139 read 35.2oC

-All jars were the same

-Added 3ml of water to each of the new room temperature jars

-Found small amounts of mold in jars 34, 38, & 39 (located primarily around calf manna)

4:30pm (J.M.)

-Cage 1: Contained six flies

-Jar 17: Contained one fly and 16 pupae

-Noticed new jars looked moist, did not add additional water

July 9, 2010; 9:20am (B.M., J.M., S.H.)

-Noticed all jars were the same as yesterday

-Cage 1: Contained seven flies

-Added water for humidity purposes to the 30oC incubator

-Added 3ml of water to room temperature jars (jars 34-39)

-Dug through jar 37 with forceps in search of larvae, found none

-Noticed more mold in room temperature jars

2:20pm (J.M.)

-All jars appeared moist, did not add additional water


July 10, 2010; 12:10pm (B.M., J.M.)


-Added 3ml of water to jars 34-39

7:30pm (J.M., B.M.)

-Jar 17: Contained two flies and 15 pupae



-Jar 20: Contained one dead fly


-Blank jar: Contained two flies

-Did not add additional water to jars 34-39

July 11, 2010; 11:02pm (B.M., J.M.)

-All jars were same as yesterday

-Added 3ml of water to jars 34-39

7:00pm (J.M., B.M.)


-Jar 18: Contained one fly and three pupae

-Jars appeared moist, did not add additional water

July 12, 2010; 10:00am (B.M., J.M.)

-Jar 17: Contained two flies and 15 pupae

Transferred jar into its own cage for easier counting




-Blank: Contained two flies


10:08pm (J.M., B.M., S.H.)

-Jar 17: Contained three flies and 14 pupae

-All other jars were the same as in the morning

-Searched through jar 38 with forceps for larvae; found nothing

-Added 3 ml of water to jars 34-39.

July 13, 2010; 10:50am (B.M., J.M., S.H.)

-Added 3 ml of water to jars 34-39

-Noticed light bulb in room 119 was burnt out; replaced light bulb

-Jar 35: Observed one larva on the side of the jar

Cage 1: Contained seven flies

-All other jars were same as yesterday

3:00pm (B.M., J.M., S.H.)

-Noticed cage 1 and all jars to be the same as morning count

July 14, 2010; 9:20am (B.M., S.H.)







-Added 3 ml of water to jars 34-39

3:00pm (B.M., S.H., J.M.)

-Noticed cage 1 and all jars to be the same as morning count

-Jars appeared moist; did not add additional water

July 15, 2010; 9:15am (J.M., B.M., S.H.)







-All jars appeared moist; did not add additional water

11:30am (J.M., S.H., B.M., H.B.)

-Cleaned out jars 34-39 since no larvae had been sighted

-Decided to try exponential cages next

-Added rehydrated liver to Cage 2 to collect eggs

2:50pm (S.H., J.M., B.M.)

-Collected/Counted eggs and placed in new setup

-New setup: Filled clear Hy-Vee salad container with calf manna/wheat bran/water mixture

Placed eggs on top of substrate

Placed lid on top (not sealed)

Put Hy-Vee contained inside of fly rearing cage (Cage 3)

Placed cage 3 inside of 35oC incubator in room 119 (changed 30

oC incubator to 35

oC)

-Noticed jars 17, 18, 19, 21, and blank to be the same as morning count

July 16, 2010; 9:05am (B.M., J.M., S.H.)






-Cage 3: No larvae spotted at 35oC

10:05am (J.M., S.H., B.M.)

-Counted 362 eggs and created a new cage (Cage 4) with same setup as 35oC incubator

-Put Cage 4 in 20oC incubator (Room 139) and added light source

1:34pm (J.M., S.H., B.M.)

-Noticed all jars and cages to be the same as morning count

-Noticed all jars and cages to be well hydrated; did not add additional moisture

July 17, 2010; 10:30am (J.M.)






-Cage 3: Spotted many larvae (mostly on lid trying to escape)

Put larvae found on bottom of cage back into plastic container

Stirred substrate

Added 5ml distilled water to substrate

-Cage 4: Noticed substrate appeared hydrates; did not add additional moisture

Saw one very small larva on underside of lid

5:15pm (P.S.)

-Noticed jars appeared hydrated

-All jars were same as morning count

-Cage 3: Noticed many larvae; all inside of container

Put larvae on underside of lid back onto substrate itself

Noticed substrate appeared moist

-Cage 4: Did not see any larvae

Noticed substrate appeared moist

July 18, 2010; 12:00pm (P.S.)

-Noticed all jars were the same as yesterday and all appeared to be well hydrated

-Cage 3: Saw many larvae (all within plastic container)

Added 3 ml of water to substrate

Noticed a small amount of mold developing

-Cage 4: Observed no larvae

Noticed a small amount of mold

Substrate appeared hydrated

10:15pm (J.M., S.H., B.M.)

-Noticed all jars to be the same as yesterday

-Cage 3: Noticed many larger larvae

Stirred substrate and added 30ml of distilled water to substrate

Noticed quite a bit of mold and took off lid to plastic container

-Cage 4: Did not observe any larvae

Added 15ml of distilled water

Observed mold growing around liver and took off lid to plastic container

July 19, 2010; 9:05am (J.M., S.H., B.M.)

-Observed all jars to be the same as yesterday

-Cage 3: Noticed top layer of substrate to be dried out


Put lid back on container (unsealed)

-Cage 4: Noticed top layer of substrate to be somewhat dried out


Put lid back on container (unsealed)

1:15pm (J.M., S.H., B.M., H.B., C.M., N.M.)

-Cage 3: Stirred substrate

-Had weekly meeting to discuss new/old business

-Made emergence trap in Cage 3 to help count adult flies when they hatch

-Funnel connecting water bottle and plastic container to allow flies to move upward into the bottle for

counting

July 20, 2010; 9:25am (J.M., H.B., B.M., S.H.)


-Cage 3: Stirred substrate and unattached lid for air flow


2:00pm (N.M.)



July 21, 2010; 11:10am (B.M., J.M., S.H.)


-Cage 1: Contained four living flies

Removed three dead flies and placed them in freezer for later gender classification

-Cage 3: Observed many larvae

Stirred substrate


Stirred substrate

2:00pm (N.M.)

-Cage 3: Observed many larvae

Substrate appeared very moist and hydrated

Stirred substrate


Stirred substrate and added 1ml of water to substrate

July 22, 2010; 9:30am (S.H., B.M., J.M.)

-Cage 1: Contained four flies

-Cage 3: Observed pupae and larvae

-Cage 4: No larvae observed

Added 20ml water to hydrate substrate

-Noticed jars 17, 18, 19, & 21 to be the same

2:00pm (N.M.)

-Cage 3: Observed many larvae and a few pupae

Added 20ml of water

-Cage 4: Added 10ml of water to substrate

July 23, 2010; 9:30am (J.M., S.H., B.M.)

-Cage 1: Contained four flies


Appeared very moist (did not add any water)


Appeared very moist (did not add any water)

4:30pm (B.M., S.H.)

-Cage 3: Added air holes to water bottle for ventilation


July 24, 2010; 2:00pm (B.M., S.H.)



July 25, 2010; 1:30am (B.M., S.H.)



9:00am (B.M., S.H.)


Added 15ml water to substrate


July 26, 2010; 10:10am (N.M.)


Noticed substrate appeared to be very moist (did not add any water)

Observed larvae and pupae


5:00pm (J.M., S.H.)

-Cage 3: Stirred substrate (very moist)

July 27, 2010; 10:00am (J.M., S.H., B.M.)

-Cage 1: Added liver to start possible exponential growth

Observed one dead fly (two living)


-Cage 4: Cleaned out and threw away

3:00pm (N.M.)


Appeared moist

July 28, 2010; 10:35am (J.M., S.H., B.M.)

-Cage 1: Observed one additional dead fly (one living)


-Jar 21: Observed one dead fly (1 living)

1:30pm (N.M.)


Added 10ml of water to substrate

Observed that pupae floated when placed in water

-Cage 2: Inserted 3x5 index card hanging from string (contained one yellow side and one white side)

July 29, 2010; 9:30am (J.M., S.H., B.M.)

-Jar 21: Observed one dead fly (0 living)

-Cage 1: Contained one fly


4:30pm (N.M.)


July 30, 2010; 9:00am (J.M., S.H., B.M.)

-Jar 17: Observed one dead fly (two living)


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