hplc, lc/msカラム inertsustain...
TRANSCRIPT
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InertSustain AQ-C18
ES 1.9 m 3 m5 m 350 m2/g 100 (10 nm) 0.85 mL/g
U S P pH
13 % L1 110
20182018201820186666
ODS
HPLC, LC/MS
InertSustain AQ-C18
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InertSustain AQ-C18
InertSustain AQ-C18InertSustainODSODSODS100%
1
100%t0ODS
InertSustain AQ-C18ODS
ConditionsColumn 5 m, 150 4.6 mm I.D.Eluent 0.1 % HCOOH in H2OFlow Rate 1.0 mL/minCol. Temp. 40 Detection UV 210 nm
Sample:1. Pyridoxamine (Vitamin B6)2. Thiamin (Vitamin B1) 3. Nicotinic acid (Vitamin B3)4. Pyridoxal (Vitamin B6)5. Nicotinamide (Vitamin B3)6. Pyridoxine (Vitamin B6)
InertSustain AQ-C18
InertSustain C18ODS
C
ODS
A
ODS
1
23
4
5 6t0
1
23+4
56
23
4
56
1
0 2 4 6 8
1
2 3+4
5 6
Time(min)
C18100%C18
HILIC
De-wetting
C18
InertSustain AQ-C18
t0
t0
t0
2
-
4
5
6
7
(min
)
2
3
4
5
(min
)
0 10 20Time (min)
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0Time (min)
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0Time (min)
InertSustain AQ-C18
ODS
InertSustain AQ-C18
InertSustain C18
Inertsil ODS-3Inertsil ODS-4
Inertsil ODS-SP
A
D
B
E
C
1 InertSustain AQ-C18
ODSInertSustain AQ-C18100 %
2
0 10 20Time (min)
InertSustain AQ-C18
0 10 20Time (min)
ODSA
ODSA
Conditions
Column
Eluent
Flow RateCol. Temp.DetectionSample
1
2
1
2
3
12
3
1
2
3
12
3
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0Time (min)
ODSC
ODSC: 5 m, 150 4.6 mm I.D.
: A) CH3CN B) 0.1 %HCOOHA/B = 40/60,v/v
: 1 mL/min: 40 : UV 310 nm1. 8-Quinolinol
2.Hinokitiol 3. Piroctone Olamine
Conditions
Column
Eluent
Flow RateCol. Temp.DetectionSample
1
2
InertSustain AQ-C18
InertSustain AQ-C18
InertSustain C18
InertSustain C18
A
A
B
B
C
C
Dopamine
Succinic acid
1
2
1
2
1
2
InertSustain AQ-C18
A
B
C
InertSustain C18InertSustain AQ-C18
2 100 %
26
3 2080
5 m, 150 4.6 mm I.D.
A) CH3CN
B) 25 mM K2HPO4 (pH 7.0, KH2PO4)
A/B = 30/70,v/v
1 mL/min
40 UV 230 nm 1.Uracil
2.Berberine chloride3.Dextromethorphan
3
-
0 2 4 6 8 10Time (min)
0 2 4 6 8 10Time (min)
InertSustain AQ-C18C18ESI-MS
InertSustain AQ-C18
2 3 45 6
1
53
4A
ConditionsColumn : 5 m ,150 4.6 mm I.D. Eluent A) 0.1 %HCOOH in H2O B) CH3CN
A/B= 80/20, v/v Flow Rate 1.0 mL/minCol. Temp.40 Detection UV 280 nm
Sample:1. Gallocatechin (GC)2. Catechin (C)3. Epicatechin (EC)4. Epigallocatechin gallate (EGCg) 5. Epicatechin gallate (ECg) 6. Catechin gallate (Cg)
62
1
ConditionsColumn Size : 50 2.1 mm I.D.Eluent : A) 0.05 % HCOOH in H2O
: B) CH3CNA/B= 70/30 - (0.5 min) - 10/90 - (0.5 min hold)
Flow Rate : 0.6 mL/minDetection : LC/MS/MS(ESI,SRM)Injection Vol. : 5 L
Sample:1. Propranolol(+)2. Carbamazepine(+) 3. Salicylic acid(-)4. Ketoprofen(-)each 100 g/L in 50 % ACN
InertSustain AQ-C181.9 m ODS1.7 m
- -
1
1
2 23
3
4 4
+Positive-Negative
UP
0.0 0.5 1.0 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
(x1,000,000)
0.0 0.5 1.0 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
(x1,000,000)
4
InertSustain AQ-C18ODSInertSustain AQ-C18
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LC/MS
PEEKInertSustain AQ-C18ATP
InertSustain AQ-C18
ConditionsColumn : InertSustain AQ-C18
(5 m, 150 4.6 mm I.D.)Eluent : 50 mM K2HPO4 in H2O (pH 7.0, H3PO4)Flow Rate : 1.0 mL/min Col.Temp. : 40 Detection : UV 260 nm Injection Vol. : 1 L
CH3CN/H2O=1/1,v/v CH3CN 100 %
Sample:1.IMP2.ATP33.ADP24.AMP15.Hyp6.Ino each 5 mg/L
0 10 20Time (min)
02
46
8m
AU
ATP
5 m, 150 4.6 mm I.D. 3 m, 150 4.6 mm I.D
ConditionsColumnEluent
Flow RateCol. Temp.DetectionInjection Vol.5 L
InertSustain AQ-C18 A) CH3CN
B) 10 mM NaH2PO4 in H2O (pH 2.5, H3PO4)1.0 mL/min40UV 210 nm
Sample1. Pyridoxamine2. Thiamin3. Nicotinic acid4. Pyridoxal 5. Nicotinamide6. Pyridoxine7. Pantothenic acid 8. Folic acid 9. Riboflavineach 20 mg/L
Time(min)
A% B%
0.0 0 10015.0 25 7515.1 0 10020.0 0 100
InertSustain AQ-C18
0.0 2.5 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
(x10,000)
0.0 2.5 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
(x10,000)
0.0 2.5 min
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
(x10,000)
InertSustain AQ-C18SUS
InertSustain C18
InertSustain AQ-C18
ConditionsColumn sizeEluent
Flow RateCol. Temp.DetectionInjection Vol.Sample
Time(min)
A% B%
0 98 20.5 98 23.0 75 25
3.01 98 27.0 98 2
: 3 m , 150 2.1 mm I.DHP: A) 5 mM Ammonium formate in H2O
B) CH3CN*: 0.3 mL/min: 40 : LC/MS/MS (ESI,SRM) , Positive: 2 L: 1.ATP 500 g/L
2.ADP 500 g/L3.AMP 500 g/L
0 2 4 6 8 10Time (min)
-0.2
0.0
0.2
0.4
0.6
0.8
mA
U1
23
4
56
3
5
42
1
1
2
3
1
2
3
1
2
3
Time(min) Time(min) Time(min)
5
0 2 4 6 8 10 12 14Time (min)
020
4060
mV
0 2 4 6 8 10 12 14Time (min)
020
4060
mV
1
34
6 7 8 92 5
1
3 4 67 8 9
25
LCNo.154
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1.9 m
/ (mm) 2.1 3.0
50 5020-89938 5020-89941 65,000
100 5020-89939 5020-89942 71,000
150 5020-89940 5020-89943 82,000
3 m
/ (mm) 1.0 1.5 30 5020-89871 5020-89877 54,00050 5020-89872 5020-89878 54,00075 5020-89873 5020-89879 63,000100 5020-89874 5020-89880 63,000150 5020-89875 5020-89881 68,000250 5020-89876 5020-89882 78,000
/ (mm) 2.1 3.0 4.0 4.6 30 5020-89831 5020-89839 5020-89847 5020-89855 46,00050 5020-89832 5020-89840 5020-89848 5020-89856 46,00075 5020-89833 5020-89841 5020-89849 5020-89857 52,000100 5020-89834 5020-89842 5020-89850 5020-89858 52,000125 5020-89835 5020-89843 5020-89851 5020-89859 56,000150 5020-89836 5020-89844 5020-89852 5020-89860 58,000250 5020-89837 5020-89845 5020-89853 5020-89861 68,000
5 m
/ (mm) 1.0 1.5 30 5020-89741 5020-89747 48,00050 5020-89742 5020-89748 48,00075 5020-89743 5020-89749 58,000100 5020-89744 5020-89750 58,000150 5020-89745 5020-89751 61,000250 5020-89746 5020-89752 69,000
/ (mm) 2.1 3.0 4.0 4.6 30 5020-89701 5020-89709 5020-89717 5020-89725 42,00050 5020-89702 5020-89710 5020-89718 5020-89726 42,00075 5020-89703 5020-89711 5020-89719 5020-89727 47,000100 5020-89704 5020-89712 5020-89720 5020-89728 47,000125 5020-89705 5020-89713 5020-89721 5020-89729 50,000150 5020-89706 5020-89714 5020-89722 5020-89730 52,000250 5020-89707 5020-89715 5020-89723 5020-89731 63,000
1/16 20MPa
UP
2.4 mm
3.3 mm
InertSustain AQ-C18
1/16 LCUPLCUP4.6 mm50MPa
6
UP80MPa
HP
3 m
/ (mm) 2.1 3.0 4.6
30 5020-89920 5020-89926 5020-89932 58,000
50 5020-89921 5020-89927 5020-89933 58,000
75 5020-89922 5020-89928 5020-89934 63,000
100 5020-89923 5020-89929 5020-89935 63,000
150 5020-89924 5020-89930 5020-89936 68,000
250 5020-89925 5020-89931 5020-89937 78,000
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(mm)(mm) (mm)
E2
EE21
3 m 5 m 3 m 5 m1.0
10
1.0 5020-89910 5020-89808 20,000 5020-89911 5020-89809 38,0001.5 2.1 1.5 5020-89912 5020-89810 20,000 5020-89913 5020-89811 38,0002.1 3.0 3.0 5020-89908 5020-89806 16,000 5020-89909 5020-89807 33,0004.0 4.6 4.0 5020-89906 5020-89804 16,000 5020-89907 5020-89805 33,0002.1 3.0
203.0 5020-89916 5020-89814 22,000 5020-89917 5020-89815 43,000
4.0 4.6 4.0 5020-89914 5020-89812 22,000 5020-89915 5020-89813 43,000
E10 mm 5020-08500 25,00020 mm 5020-08550 28,000
InertSustain AQ-C18 E
1/16 20 MPa
for UHPLC80 MPa
1/160.18 mm30 mm2.4mm1/16
https://www.gls.co.jp/
PEEK
3 m
mmmm
2.1 4.6
1.9 m50 5020-87068 5020-87065 96,000100 5020-87069 5020-87066 100,000150 5020-87070 5020-87067 102,000
3 m
50 5020-87061 5020-87057 85,000100 5020-87062 5020-87058 89,000150 5020-87063 5020-87059 91,000250 5020-87064 5020-87060 102,000
5 m
50 5020-87053 5020-87049 61,000100 5020-87054 5020-87050 78,000150 5020-87055 5020-87051 78,000250 5020-87056 5020-87052 88,000
PEEK
E
for UHPLC
PEEK5PEEKPEEK80 MPaUHPLC-PEEK20 MPaPEEK2InertSustain AQ-C18InertSustainInertsil
80 MPa
20 MPa
UP
7
(mm)
(mm)
(mm)
2 2+1
1.9 m 3 m 1.9 m 3 m
1.0
10
1.5 5020-89944 27,000 5020-89824 25,000 5020-89947 43,000 5020-89827 41,0001.52.1 2.1 5020-89945 27,000 5020-89825 25,000 5020-89948 43,000 5020-89828 41,0002.13.0 3.0 5020-89946 25,000 5020-89826 22,000 5020-89949 41,000 5020-89829 40,000
for UHPLC 5020-08630 25,000
5 m
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https://www.gls.co.jp/
AA851-20180615PDF