Hybrid Multi-Mode Microplate Reader

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3 Multi-Volume Multi-Sample Plate with 2 µL

microspots Specifications and Summary

Synergy™ H4: Hybrid Technology

Filter-based optics: Use of dichroic mirrors increases

sensitivity about 5x compared to filter-only optics

Peak transmission for filters and mirrors is around 80%. Very efficient optical system

Because of high amount of energy delivered to samples, high sensitivity can be obtained with short read times

In summary, a fast and very sensitive optical system

Synergy™ H4: Hybrid Technology

Monochromator-based optics: Use of two gratings per

monochromator provides smooth performance from 250 to 900 nm

Very large range of applications, including spectral scanning

Variable bandpass system adds flexibility

In summary, an extremely flexible optical system

%CV Assay Window Z'

Filter

Monochromator

Synergy™ H4: Hybrid Technology

Patent-pending design: Flexible monochromator system High-performance filter system Each with its strengths and

weaknesses Extremely versatile instrument

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Filter wheels: Easy front access No tool required Easy load/unload software function

Dichroic mirrors: Easy side access Easy load/unload

software function

High-performance, easy-to-use filter system

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

300 µl200 µl 100 µl

300 µl200 µl 100 µl

Top fiber

Automated Z-Focusing

If no Z-focusing available, performance issues in low volumes, or use of adapters required.

With Z-focusing, reading height can be precisely adjusted (100 µm resolution) for best performance on all plate types, and with all volumes.

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Time (minutes)F

luo

resc

en

ce (

RF

U)

0:00:00 0:01:00 0:02:00 0:03:00 0:04:00 0:05:00 0:06:00 0:07:00 0:08:005000

10000

15000

20000

25000

30000

35000

Baseline Response

Trigger Reagent Injection

Dual Reagent Dispenser Option

Two reagent dispensers Volume range 5 µL - 1000 µL Enables dispense/read processes Ion channel assays, flash

luminescent assays, time-sensitive kinetic assays

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

34

12

Advanced 4-ZoneTM Temperature Control System

4 independent heaters with 4 monitoring sensors

Precision: +/- 0.5°C at 37°C Range: RT to 65°C Enables applications with strict

temperature uniformity requirements (e.g. ORAC assays)

Enables high-temperature assays (e.g. Invader assays at 62°C)

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Sixteen 2 µL microspots Enables low volume A260 nucleic

acid quantification dsDNA detection limit 2 ng/ µL Can also be used for A280

protein quantification, as well as fluorescence or luminescence detection

Take3™ Multi-Volume Multi-Sample Plate with 2 µL microspots

Contents

Synergy H4 – Hybrid Technology Key Features and Benefits

Quadruple Grating ArchitectureVariable Band-Pass SelectionHigh-Performance, Easy-to-Use Filter SystemAutomated Z-FocusingDual Reagent Dispenser OptionTemperature Control System to 65°CTake3™ Multi-Volume Plate with 2 µL microspots

Specifications and Summary

Summary: Versatility Redefined

Hybrid Technology: Monochromators and Filters Quadruple Grating Optical Architecture Variable Bandpass Selection Dichroic Mirrors and Filters for High Performance Dual Reagent Dispenser Option Temperature Control System to 65°C Take 3™ Multi-Volume Plate with 2 µL microspots

Visit www.biotek.com for more information